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Infection and Immunity, July 2009, p. 2635-2642, Vol. 77, No. 7
0019-9567/09/$08.00+0     doi:10.1128/IAI.00077-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Salmonella enterica Serovar Typhimurium Pathogenicity Island 1-Encoded Type III Secretion System Translocases Mediate Intimate Attachment to Nonphagocytic Cells{triangledown} ,{dagger}

María Lara-Tejero and Jorge E. Galán*

Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Ave., New Haven, Connecticut 06536

Received 20 January 2009/ Returned for modification 28 February 2009/ Accepted 3 April 2009

Delivery of bacterial proteins into mammalian cells by type III secretion systems (TTSS) is thought to require the intimate association of bacteria with target cells. The molecular bases of this intimate association appear to be different in different bacteria involving TTSS components, as well as surface determinants not associated with TTSS. We show here that the protein translocases SipB, SipC, and SipD of the Salmonella enterica serovar Typhimurium pathogenicity island 1 (SPI-1)-encoded TTSS are required for the intimate association of these bacteria with mammalian cells. S. Typhimurium mutant strains lacking any of the translocases were defective for intimate attachment. Immunofluorescence microscopy showed that SipD is present on the bacterial surface prior to bacterial contact with host cells. In contrast, SipB and SipC were detected on the bacterial surface only subsequent to bacterial contact with the target cell. We conclude that the coordinated deployment and interaction between the protein translocases mediate the SPI-1 TTSS-dependent intimate association of S. Typhimurium with host cells.

* Corresponding author. Mailing address: Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Ave., New Haven, CT 06536. Phone: (203) 737-2404. Fax: (203) 737-2630. E-mail: jorge.galan{at}yale.edu

{triangledown} Published ahead of print on 13 April 2009.

{dagger} Supplemental material for this article may be found at http://iai.asm.org/.

Editor: A. Camilli

Infection and Immunity, July 2009, p. 2635-2642, Vol. 77, No. 7
0019-9567/09/$08.00+0     doi:10.1128/IAI.00077-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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