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Infection and Immunity, August 2009, p. 3312-3319, Vol. 77, No. 8
0019-9567/09/$08.00+0     doi:10.1128/IAI.00013-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Uropathogenic Escherichia coli-Induced Inflammation Alters Mouse Urinary Bladder Contraction via an Interleukin-6-Activated Inducible Nitric Oxide Synthase-Related Pathway{triangledown}

Te I. Weng,1,3 Hsiao Yi Wu,1,{dagger} Pei Ying Lin,2,{dagger} and Shing Hwa Liu2,4*

Department of Forensic Medicine,1 Institute of Toxicology, College of Medicine, National Taiwan University,2 Departments of Emergency Medicine,3 Surgery, National Taiwan University Hospital, Taipei, Taiwan4

Received 5 January 2009/ Returned for modification 17 February 2009/ Accepted 14 May 2009

Escherichia coli is the most common cause of urinary tract infection. Elevated blood and urine interleukin-6 (IL-6) levels have been shown in inflammatory urinary tract diseases. The role of IL-6 in mediating the urodynamic dysfunction in response to E. coli-induced urinary tract infection has not yet been fully elucidated. In this study, we investigated the role of IL-6 in the nitric oxide (NO)-triggered alteration of contractile responses in the urinary bladder under an E. coli-induced inflammatory condition. The electrical field stimulation (EFS)-evoked contractions of the isolated detrusor strips, and immunoblotting for detecting protein expression in the bladders was measured short term (1 h) or long term (6 or 24 h) after intraperitoneal injection of E. coli endotoxin (lipopolysaccharide [LPS]) or intravesical instillation of human pyelonephritogenic E. coli-J96 (O4:K6) strain or LPS into mice. IL-6 and NO productions were increased in the urinary bladders of mice 1 to 24 h after LPS or E. coli-J96 treatment. Inducible NO synthase (iNOS) expression and protein kinase C (PKC) activation and EFS-evoked detrusor contractions were increased in the bladders at 6 h after LPS or E. coli-J96 treatment, which could be reversed by anti-IL-6 antibody and iNOS inhibitor aminoguanidine. At 1 h after LPS administration, bladder NO generation, endothelial NOS expression, and EFS-evoked detrusor contractions were effectively increased, whereas anti-IL-6 antibody could not reverse these LPS-induced responses. These results indicate that IL-6 may play an important role in the iNOS/NO-triggered PKC-activated contractile response in urinary bladder during E. coli or LPS-induced inflammation.

* Corresponding author. Mailing address: Institute of Toxicology, College of Medicine, National Taiwan University, No. 1, Section 1, Jen-Ai Road, Taipei 10051, Taiwan. Phone: (886)-2-23123456, ext. 88605. Fax: (886)-2-23410217. E-mail: shinghwaliu{at}ntu.edu.tw

{triangledown} Published ahead of print on 26 May 2009.

Editor: R. P. Morrison

{dagger} H.Y.W. and P.Y.L. contributed equally to this study.

Infection and Immunity, August 2009, p. 3312-3319, Vol. 77, No. 8
0019-9567/09/$08.00+0     doi:10.1128/IAI.00013-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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