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Infect Immun. 1974 May; 9(5): 934-938
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Deoxyribonucleic Acid Base Composition and Hybridization Studies on the Human Pathogen Sporothrix schenckii and Ceratocystis Species

Leda C. Mendonça-Hagler, L. R. Travassos, K. O. Lloyd and H. J. Phaff

Department of Food Science and Technology, University of California, Davis, California 95616
Departments of Dermatology and Biochemistry, College of Physicians and Surgeons, Columbia University, New York, New York 10032

ABSTRACT

Deoxyribonucleic acid (DNA) base composition, as measured by guanine plus cytosine (G + C) content, was determined in 17 strains of Sporothrix schenckii and Ceratocystis species. The average G + C content for S. schenckii was 54.7 ± 0.2 mol%. No strain of Ceratocystis, with the exception of C. minor, gave values for G + C content similar to those obtained for S. schenckii. All DNA samples from strains of S. schenckii cross-hybridized with high percentages of relative binding. DNA samples from C. stenoceras and C. pilifera showed low degrees of homology with S. schenckii DNA. The DNA from C. minor hybridized with 75% binding with that from a strain of S. schenckii, as compared with the homologous reaction.

Infect Immun. 1974 May; 9(5): 934-938
Copyright © 1974 American Society for Microbiology. All Rights Reserved.



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