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Infect Immun. 1974 May; 9(5): 974-976
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Scanning Density Gradient Isoelectric Focusing of Staphylococcus aureus Enterotoxins B and C₁

Department of Microbiology, Loyola University Medical Center, Maywood, Illinois 60153
Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Food Research Institute, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

Purified Staphylococcus aureus enterotoxins B and C₁, the causative agents of staphylococcal food poisoning, were resolved into several components by scanning isoelectric focusing in sucrose density gradients in the absence and presence of 6 M urea and 5 mM dithiothreitol. The observed heterogeneity persisted in the denaturing medium, which suggests that the primary structure of protein enterotoxins B and C₁ may be partly responsible for the isoelectric point differences of the various forms. To our knowledge, the scanning isoelectric focusing patterns of staphylococcal enterotoxins B and C₁ are reported for the first time.