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Infect Immun. 1974 June; 9(6): 1051-1056
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Inhibition of Phagocytosis-Associated Chemiluminescence by Superoxide Dismutase

Departments of Pediatrics and Microbiology, Institute of Dental Research, and Cancer Research and Training Center, The University of Alabama in Birmingham, Birmingham, Alabama 35294

ABSTRACT

During the process of phagocytosis, human leukocytes emit a burst of luminescence which can be measured in a liquid scintillation spectrometer. The enzyme superoxide dismutase, which removes superoxide anions (O_·), inhibited this chemiluminescence by 70% at a concentration of 100 µg/ml. The enzyme did not inhibit phagocytosis. These results support other studies indicating that O_· is elaborated by phagocytizing leukocytes. They also indicate that O_· plays a major role in phagocytosis-associated chemiluminescence, though not necessarily as the luminescing agent. Catalase and benzoate inhibited the chemiluminescence of phagocytosis to a slight extent, suggesting that hydrogen peroxide and hydroxyl radical, respectively, might also be involved in this phenomenon. The relationship between the mediators of chemiluminescence and those responsible for phagocytic bactericidal activity remains to be defined.

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