This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Sharma, J. M. Articles by Purchase, H. G. Search for Related Content PubMed PubMed Citation Articles by Sharma, J. M. Articles by Purchase, H. G.

 Previous Article  |  Next Article 

Infect Immun. 1974 June; 9(6): 1092-1097
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Replication of Marek's Disease Virus in Cell Cultures Derived from Genetically Resistant Chickens

Regional Poultry Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, East Lansing, Michigan 48823

ABSTRACT

Various parameters of replication of Marek's disease virus (MDV) were studied in cells derived from genetically resistant (line 6) and susceptible (line 7) chickens. Cell-free and cell-associated preparations of three strains of pathogenic MDV (JM, GA, and Id-1) replicated equally well in cells of resistant and susceptible chickens. There were no demonstrable differences between the two genetic sources of cells for the chronological appearance and type of cytopathic effects and for the type of virus-induced antigens detectable by immunofluorescent and agar-gel precipitin tests. MDV propagated for about 4 weeks in cells of resistant line 6 chickens remained nonvirulent for line 6 and fully virulent for line 7 chickens. The lack of expression of genetic resistance to Marek's disease at cellular level contrasts the infection mechanisms of MDV from those of viruses associated with lymphoid leukosis in chickens.