Immunogenicity and protective efficacy of recombinant Campylobacter flagella-secreted proteins in mice

Enteric Diseases Department, Naval Medical Research Center, Silver Spring, Maryland

^* To whom correspondence should be addressed. Email: shahida.baqar{at}med.navy.mil.

	Abstract

Immunogenicity and protective efficacy of three Campylobacter jejuni flagella-secreted proteins FlaC, FspA1 and FspA2 were compared in a mouse model. Mice were immunized intranasally with each protein with or without LTR192G as adjuvant, and challenged intranasally with C. jejuni 81-176 or CG8486. All three proteins were immunogenic, although FspA1 induced the highest levels of serum IgG and fecal IgA. Although immunogenic, FlaC provided only 18% protection against disease from C. jejuni 81-176. Immunization with FspA1 resulted in 57.8% protection without adjuvant or 63.8% protection with adjuvant against homologous challenge with 81-176. Alternatively, immunization with FspA2 provided 38.4% (without adjuvant) or 47.2% (with adjuvant) protection against disease from homologous challenge with CG8486. In contrast to FspA2, FspA1 provided some heterologous protection against C. jejuni CG8486 when delivered with (31.2%) or without (44.8%) LTR192G. These results suggest FspA1 may be a good subunit vaccine candidate against C. jejuni disease.