INTRODUCTION

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Nontyphoidal serovars of Salmonella spp. which possess related virulence plasmids have the potential to cause systemic disease, particularly in immunocompromised humans (65). In a mouse model, these virulence plasmids are essential for systemic infection within a week after oral inoculation (29). By genetic analysis of virulence genes on the plasmids, five spv genes, spvRABCD, were identified (29) and were sufficient to express the virulence phenotype of systemic infection for Salmonella typhimurium (26). We determined that the spv genes of S. typhimurium primarily enabled more rapid growth rate in mice but did not significantly affect killing or movement through tissues, by using a temperature-sensitive genetic marker to measure the relative number of bacterial cell divisions in vivo (30). In the natural infection, the bacteria enter the host by the oral route and invade the intestinal epithelial cells and/or M cells (3) in a plasmid-independent manner (28). Salmonellae then invade and proliferate in Peyer's patches and mesenteric lymph nodes (3). The bacteria reach the liver and spleen through the lymphatics and blood. The virulence plasmid is not necessary for infection of the intestines, resistance to complement-mediated bacteriolysis of serum, resistance to phagocytosis and killing by macrophages, or adherence to, invasion into, and growth within certain cell lines in vitro (28, 29).

Since the spv genes affect the virulence of salmonellae primarily in lymphoid tissues, many investigators have proposed that the Spv phenotype is manifested in phagocytes, primarily macrophages. However, until recently (53), direct proof of this hypothesis has been lacking. In fact, irrespective of the role of the spv genes in salmonella virulence, the cellular location of salmonellae in the host has been controversial. Most reports support infection of macrophages as essential for salmonella virulence (15, 23, 68). However, others propose that salmonellae either are extracellular (35, 41) or infect nonphagocytic cells (6, 8) or polymorphonuclear leukocytes (PMNs) (11). Ultimately, a comprehensive histological analysis of infected tissues from mice that were inoculated in a relevant manner with a relevant inoculum will be required to settle these controversies.

We pursued a biological approach to examine the interaction of Spv⁺ and Spv S. typhimurium with different populations of host cells. We used mice genetically deficient for lymphocytes, mice depleted of phagocytes by different drugs, and mutant S. typhimurium strains that were rendered defective for infecting nonphagocytic cells. Our results presented here indicate that invasion of nonphagocytes is irrelevant for virulence of either Spv⁺ or Spv salmonellae during infection beyond the intestines, and that T cells and B cells have no detectable role in suppressing or enabling systemic infection by S. typhimurium within 5 days after oral inoculation. PMNs had a variable role in suppressing overall salmonella infection but did not differentially suppress Spv salmonellae. However, quantitative depletion of macrophages from mice by using drugs rendered Spv⁺ and Spv S. typhimurium equal for systemic infection. Together, these data indicate that within a week after oral inoculation of BALB/c mice the spv genes increase the growth rate of salmonellae within macrophages and suggest that macrophages are the only relevant host cells for replication of salmonellae beyond the intestines.

	MATERIALS AND METHODS

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Bacterial strains and culture. The S. typhimurium strains used in this study are described in Table 1. Spv⁺ virulence plasmid-containing S. typhimurium SR-11, 3456 (tetracycline resistant), 3306 (nalidixic acid resistant), and isogenic Spv virulence plasmid-cured 3337 have been described elsewhere (28). A more isogenic Spv strain was constructed in which the 6.3-kb ClaI fragment encoding spvRABCD' was deleted from the virulence plasmid and replaced with the tet gene of pBR322. Construction of this strain, described elsewhere (43), involved construction of the mutation in a cloned spv sequence and recombining the mutation into 3181 by using the suicide allelic exchange vector pCVD442 (10). The invA::aphT mutation originally constructed in S. typhimurium SB147 (21) was transduced into 3456 and 3337 by using generalized transduction with P22HTint (56) grown on SB136, yielding UF102 and UF103, respectively.

Infection of mice. All of these studies used specific-pathogen-free BALB/c mice, which are sensitive to infection by S. typhimurium because of the Ity^s mutation (57). Mice were orally inoculated with S. typhimurium as described previously (27). Approximately 10⁸ CFU of S. typhimurium was fed to 7- to 11-week-old female BALB/c mice (Charles River, Wilmington, Mass., and University of Florida Department of Pathology, Immunology, and Laboratory Medicine Mouse Facility) after food and water deprivation and feeding of bicarbonate. Unless noted otherwise, 4 or 5 days later, spleens and livers were removed, homogenized in glass tissue homogenizers with phosphate-buffered saline (PBS) containing gelatin (BSG) (9), and plated to enumerate CFU. Nude BALB/c mice (Charles River) and SCID BALB/c mice (Jackson Laboratory, Bar Harbor, Maine; maintained at the University of Florida Department of Pathology, Immunology, and Laboratory Medicine Mouse Facility) were also used with oral inoculation. Alternatively, mice were inoculated subcutaneously (s.c.) into both hind footpads as described previously (30) with 10⁵ CFU of salmonellae suspended in 0.02 ml of BSG per footpad. Three or four days later, spleens were removed, homogenized in BSG, and plated to enumerate CFU. Mice were either inoculated with a single salmonella strain or equal mixtures of two strains as described (30).

Treatment of mice with cyclophosphamide. To examine the effects of PMNs, mice were treated with cyclophosphamide as described previously (45). Cyclophosphamide (Sigma Chemical Co., St. Louis, Mo.) was dissolved in sterile, pyrogen-free distilled water or saline and injected intraperitoneally (i.p.) at 3 days preinfection and on the day of infection at a dose of 150 mg/kg of body weight. Peripheral blood leukocytes (WBC) were enumerated by hemocytometer counts of EDTA-anticoagulated whole blood diluted in 3% (vol/vol) acetic acid. Differential WBC counts were performed on Wright-Giemsa (Camco Quik Stain II; Baxter, McGaw Park, Ill.)-stained smears. Absolute neutrophil counts were calculated at the time of necropsy by multiplying the total WBC by the percentage of neutrophils obtained from differential counts (45). We routinely observed at least 90% reduction of peripheral blood PMNs after cyclophosphamide treatment.

Treatment of mice with liposomes containing dCMdP. To examine the effects of functional depletion of macrophages, mice were treated with liposomes containing dichloromethylene diphosphate (dCMdP) as described by van Rooijen (61). Briefly, liposomes consisted of 87% (wt/wt) phosphatidylcholine and 13% (wt/wt) cholesterol (Sigma). dCMdP (a gift of Boehringer Mannheim) was dissolved in PBS at a concentration of 18.9% (wt/vol) and was incorporated into liposomes by sonication. Liposomes were sized to 150 to 200 nm and washed in PBS before use in mice. Mice were injected intravenously (i.v.) in the lateral tail vein with approximately 4 mg of dCMdP contained in 0.2 ml of liposome suspension. For initial experiments using liposome-dCMdP alone, injection was on the day before inoculation with salmonellae. For subsequent experiments with liposome-dCMdP and experiments involving combined cyclophosphamide-liposome-dCMdP treatment, injection of liposomes was on the day of oral inoculation at the time of preinoculation food and water deprivation. Liposomes containing only PBS and occasionally PBS without liposomes were used as negative controls.

Immunohistochemical analysis of mouse tissues. To confirm the effects of depleting mice of PMNs by using cyclophosphamide or depleting macrophages with liposome encapsulated dCMdP, a portion of infected mouse spleens and livers was quick frozen in OCT embedding medium (TisTek; Sakura Finetek, Torrance, Calif.), while the remainder was homogenized and plated as described above. Tissues were sectioned in 5-µm sections and mounted at the University of Florida Department of Pathology, Immunology, and Laboratory Medicine Diagnostic Referral Laboratory and stored at 70°C. On the day of analysis, sections were thawed and fixed in absolute methanol containing 1% (vol/vol) hydrogen peroxide to block endogenous peroxidase activity. Endogenous biotin was blocked with Biotin Blocking System (Dako Corp., Carpenteria, Calif.). Fc activity was blocked with 10% (vol/vol) normal porcine serum. Primary antibodies consisted of rabbit anti-mouse macrophage serum (Inter-Cell Technologies, Hopewell, N.J.) followed by porcine anti-rabbit immunoglobulin G (IgG) conjugated with peroxidase (Dako) and then rabbit peroxidase-antiperoxidase (Harlan Bioproducts, Indianapolis, Ind.); rat monoclonal antibody FA/11 directed against the CD68 pan-macrophage, macrophage-specific antigen (22, 51) followed by donkey anti-rat IgG conjugated with peroxidase (Jackson ImmunoResearch); or rat monoclonal antibody RB6-8C5 against granulocytes (Gr-1 antigen) (17) conjugated with biotin (CalTag) followed by avidin conjugated to peroxidase (Biostain Super ABC Basic Detector; Biomeda, Foster City, Calif.). Peroxidase-conjugated complexes were developed with diaminobenzidine (Metal Enhanced DAB Substrate kit; Pierce, Rockford, Ill.), and slides were counterstained with hematoxylin. Negative control antibodies were rat antidinitrophenol monoclonal antibody conjugated with biotin (CalTag) for RB6-8C5 and rat IgG1 monoclonal antibody R187 directed against the murine leukemia virus p30^gag protein (5).

Statistical analysis. Analysis of bacterial numbers as mean log₁₀ ± standard deviation CFU was performed as described previously (2). In experiments in which individual mice were inoculated with single strains, the difference between the mean log₁₀ CFU of different groups was determined by using the Student t test. For analysis of mixed infections, the Student t test was used to examine the mean paired difference in log₁₀ CFU of the strains being different from 0. In all cases, two or three experiments were performed, and results were pooled for analysis when indicated.

	RESULTS

Top Abstract Introduction Materials & Methods Results Discussion References

Our previous results established that the spv genes of the S. typhimurium virulence plasmid primarily increase the growth rate of salmonellae during infection of host cells in infected mice (30). The central question was then which cells acted as the primary hosts for Spv-mediated increased growth and which cells, if any, acted as secondary effectors which suppressed the replication of the Spv salmonellae. To gain insight into the function of the spv genes, we examined how the mouse host could be altered so that Spv salmonellae would no longer be suppressed relative to Spv⁺ salmonellae, that is, so that the suppressive environment to which the Spv bacteria were susceptible would be eliminated. The objective of most of the experiments described below, therefore, consisted of infecting mice depleted of certain cells, either pharmacologically or by genetic mutation, with Spv⁺ and/or Spv S. typhimurium. If the primary or secondary host cells responsible for suppression of Spv salmonellae were eliminated, the Spv strain would be recovered from deep tissues such as the spleen and liver in the same or nearly the same numbers as the Spv⁺ parent.

Lymphocytes do not have a role in restricting the growth of Spv⁺ or Spv S. typhimurium within 5 days after oral inoculation. We considered the possibility that T cells and/or B cells were involved in suppressing the replication of Spv S. typhimurium; i.e., the spv genes are involved either in increased replication within these lymphocytes or in overcoming lymphocyte-mediated suppression of salmonella replication within another host cell. To examine the role of T cells in differentially suppressing plasmid-cured S. typhimurium, nude BALB/c and normal BALB/c mice were inoculated orally with 10⁸ CFU of both wild-type 3456 and plasmid-cured 3337 in mixed infections. Five days later, spleens were examined for total CFU of each strain (Fig. 1A). There was no effect of the nude BALB/c background on recovery of either Spv⁺ or Spv S. typhimurium compared with normal BALB/c mice. Furthermore, the mean paired differences between plasmid-containing and plasmid-cured salmonellae were unchanged between normal and nude BALB/c mice. Therefore, T cells do not significantly affect splenic infection within 5 days of oral inoculation and have no role in the Spv phenotype.

View larger version (37K): [in this window] [in a new window]   FIG. 1.   Lack of effect of the nude and SCID mutations on the splenic infection of BALB/c mice inoculated orally with Spv+ and Spv S. typhimurium. (A) Normal and nude BALB/c mice were orally inoculated with mixtures of wild-type, Spv+ 3456 and virulence plasmid-cured, Spv 3337. (B) Normal and SCID BALB/c mice were orally inoculated with mixtures of wild-type 3306 and spv::tet, Spv UF110. Five days later, CFU of each strain in spleens were enumerated, and the mean paired difference (Diff.) between the Spv+ and Spv strains was calculated. Standard deviations are shown as vertical lines, and n for each infection is shown above the data for the mean paired difference. These are the combined results of at least two experiments each for nude and SCID BALB/c mice. The log10 CFU recoveries for either salmonella strain between either nude and SCID BALB/c mice and the matched normal BALB/c mice were not significant (P > 0.2). The mean paired differences between Spv+ and Spv strains were always significantly greater than 0 (P < 0.005 to P < 0.001).

Neutrophils have a variable role in suppressing Spv⁺ and Spv S. typhimurium but do not affect the Spv phenotype. It has been proposed that PMNs are the relevant site of intracellular residence for wild-type S. typhimurium in the spleens of mice shortly after i.v. infection (11) and are involved in suppressing infection of hepatocytes after i.v. inoculation (6, 8). To examine the role of PMNs in suppressing intracellular growth of plasmid-cured salmonellae relative to wild-type salmonellae, mice were treated i.p. with the chemotherapeutic agent cyclophosphamide to induce granulocytopenia (45). At necropsy, we routinely observed at least a 90% decrease in peripheral blood granulocytes after cyclophosphamide treatment compared to mice treated i.p. with PBS. Furthermore, no PMNs were detectable in spleens and livers of cyclophosphamide-treated mice, using monoclonal antibody RB6-8C5 directed against the granulocyte-specific antigen Gr-1 (Fig. 2E). Mice were orally inoculated with single or mixed strains of wild-type (3456 or 3306) and/or Spv (3337 or UF110) S. typhimurium and were examined for splenic infection 4 to 5 days after inoculation.

View larger version (117K): [in this window] [in a new window]   FIG. 2.   Immunochemical analysis of PMNs and macrophages in spleens of normal and cyclophosphamide-treated mice infected with a mixture of Spv+ and Spv S. typhimurium. Mice were treated with cyclophosphamide or PBS and orally inoculated with S. typhimurium as described in the text. Four days later, spleens and livers (not shown) were frozen in OCT and sectioned for immunochemical analysis of PMNs and macrophages as described in the text. Hamster anti-mouse granulocyte monoclonal antibody was RB6-8C5 (A, E, and G), and rat anti-mouse macrophage monoclonal antibody was FA/11 (B, F, and H). RB6-8C5 was conjugated with biotin and detected with avidin-horseradish peroxidase. FA/11 was detected using donkey anti-rat IgG conjugated with peroxidase. Reactions were developed with diaminobenzidine. Negative control antibodies were rat anti-murine leukemia virus p30gag protein (5) (C) and rat antidinitrophenol (D) and consistently produced clean reactions. Sections were counterstained with hematoxylin. All images are at a magnification of ×200. (A to D) Normal mouse spleen demonstrating specificity of the primary antibodies and lack of reactivity for the negative control antibodies; (E and F) spleen from infected mouse treated with cyclophosphamide for depletion of PMNs; (G and H) spleen from infected mouse injected with PBS in place of cyclophosphamide. Note the absence of PMNs in panel E and the continued presence of macrophages in panel F.

View larger version (33K): [in this window] [in a new window]   FIG. 3.   Depletion of PMNs by cyclophosphamide treatment does not affect the Spv virulence phenotype of S. typhimurium after oral inoculation of mice. Cyclophosphamide (Cyclo.) at a dose of 150 mg/kg of body weight or PBS (Control) was injected i.p. at 3 days preinfection and on the day of infection. Mice were orally inoculated with Spv+ 3306 and spv::tet Spv UF110 at a dose of 108 CFU. Four days later, spleens and livers were examined for salmonella CFU, with the exception of two cyclophosphamide-treated mice in panel B, which were harvested 18 h earlier. Data shown are for total CFU/spleen or CFU/gram of liver. The mean paired difference (Diff.) was calculated between the salmonella strains for each mouse. Each bar represents the mean log10 CFU with standard deviation shown. The number of mice is indicated above the mean paired difference. The mean paired differences between Spv+ and Spv salmonellae were significantly greater than 0 in every case (P < 0.05 to P < 0.001). (A) Representative data from a series of three experiments in which treatment with cyclophosphamide did not result in a significant change in CFU of either Spv+ or Spv salmonellae or the mean paired difference between the strains (P > 0.1 to P > 0.5). (B) Representative data from a series of three experiments in which treatment with cyclophosphamide resulted in significant increases in CFU for both Spv+ and Spv salmonellae (P < 0.01 to P < 0.001), but the mean paired difference between the strains was not significantly changed (P  0.2).

Macrophages are essential for suppression of Spv S. typhimurium. To examine the role of macrophages in suppressing the growth of Spv S. typhimurium, mice were treated in a variety of ways which have been used by several investigators to physically or functionally deplete mice of macrophages. These treatments included i.v. injection of silica (1, 50) and i.v. injection of liposomes containing dCMdP (59, 61). After treatment of mice to deplete macrophages, mice were orally inoculated with Spv⁺ and/or Spv S. typhimurium, and splenic CFU were measured 4 to 5 days later. With these treatments, we observed that both Spv⁺ and Spv strains were increased for splenic infection 10- to 100-fold. However, the recoveries of the two strains relative to each other either remained the same or the difference increasedapproximately 100-fold-higher numbers of Spv⁺ than Spv salmonellae (data are provided for liposome-dCMdP treatment in Fig. 4). Injection of liposome-PBS resulted in splenic infection no different than injection of PBS alone (Fig. 4). The mean paired difference between Spv⁺ 3456 and Spv 3337 was unchanged as a result of liposome-dCMdP treatment (Fig. 4). Our initial interpretation was that macrophages were essential for suppression of systemic disease by S. typhimurium but that macrophages were not involved in differential suppression of replication of Spv S. typhimurium. However, when we stained macrophages in frozen sections of infected mouse tissues with rabbit anti-murine macrophage antiserum and later with monoclonal antibody FA/11 (22, 51), we observed that treatment with silica or liposome-dCMdP failed to completely deplete infected spleens of macrophages (data are shown for liposome-dCMdP in Fig. 5A and B). We further reasoned that although the treatments may have initially depleted resident macrophages, the increased systemic infection with salmonellae may have elicited monocytes and macrophages from the bone marrow. In fact, Samsom et al. showed that liposome-dCMdP treatment does not inhibit the elicitation of macrophages as part of an inflammatory response (55). We therefore treated mice with liposome-dCMdP on the day of inoculation and 2 days later to deplete any elicited macrophages. However, even with this double treatment, spleens still contained readily detectable macrophages, and the differential growth yields persisted between Spv⁺ and Spv salmonellae (data not shown).

View larger version (22K): [in this window] [in a new window]   FIG. 4.   Treatment of mice with liposome-dCMdP alone increases splenic infection by Spv+ and Spv S. typhimurium but does not relieve differential suppression of Spv S. typhimurium. Mice were injected i.v. with PBS, liposome (Lip.)-PBS, or liposome-dCMdP 1 day before oral inoculation with mixtures of Spv+ 3456 and Spv 3337 as described in the text. Four days later, spleens were removed, and CFU were enumerated. Data are mean log10 CFU with standard deviations shown, and the mean paired difference between 3456 and 3337 (Diff.) is shown (n = 4 for all groups). In all three sets, the mean paired difference is significantly greater than 0 (P < 0.05 to P < 0.005). Liposome-dCMdP treatment resulted in significantly increased splenic infection for both 3456 and 3337 compared with PBS treatment or liposome-PBS treatment (P < 0.05), except for 3337, which was not significantly increased compared with liposome-PBS. Note that as shown in Fig. 5, liposome-dCMdP treatment did not completely eliminate splenic macrophages.

View larger version (145K): [in this window] [in a new window]   FIG. 5.   Immunochemical analysis of PMNs and macrophages in spleens of liposome-dCMdP and control treated mice infected with wild-type S. typhimurium. Spleens and livers (not shown) were prepared as described for Fig. 2 with rat anti-mouse granulocyte monoclonal antibody RB6-8C5 (A, C, and E) and rat anti-mouse macrophage monoclonal antibody FA/11 (B, D, and F). All images are at a magnification of ×200. (A and B) Spleen from infected mouse treated with PBS; (C and D) spleen from infected mouse treated with liposome-PBS; (E and F) spleen from infected mouse treated with liposome-dCMdP for depletion of macrophages. Note the continued presence of PMNs and macrophages with all treatments. Reactions from the negative control antibodies were clean (data not shown).

View larger version (110K): [in this window] [in a new window]   View larger version (93K): [in this window] [in a new window]   FIG. 6.   Immunochemical analysis of PMNs and macrophages in spleens and livers of cyclophosphamide-liposome-dCMdP and control treated mice. Mice were treated with cyclophosphamide-liposome-dCMdP or PBS-liposome-PBS and orally infected with Spv+ 3456 or Spv UF110 (not shown) as described in the text. Three days later, spleens and livers were prepared as described for Fig. 2 with rat anti-mouse granulocyte monoclonal antibody RB6-8C5 (A, B, E, and F) and rat anti-mouse macrophage monoclonal antibody FA/11 (C, D, and G to K). Magnifications: A to H, are ×200; I to K, ×1,000. Images show spleens (A, C, and J) and livers (B and D) from infected mice treated with PBS/liposome-PBS, spleens (E, G, and K) and livers (F and H) from infected mice treated with cyclophosphamide-liposome-dCMdP for depletion of macrophages, and spleen from a normal, uninfected mouse (I). Note the absence of PMNs in panels E and F and the lack of macrophages in panel H. The FA/11-staining material in panel G is debris, not intact macrophages, as determined by examination at higher power in panel K. Note the lack of nuclei associated with the FA/11-staining debris in panel K compared with the associated nuclei and cellular staining in panels I and J.

View larger version (22K): [in this window] [in a new window]   FIG. 7.   Combined treatment with cyclophosphamide-liposome-dCMdP results in equivalent splenic infection by Spv+ and Spv S. typhimurium. Mice were treated with cyclophosphamide-liposome-dCMdP (Cyc./Lip.-dCMdP) or PBS-liposome-PBS (Control) as described in the text and infected with Spv+ and/or Spv S. typhimurium. (A) Single-strain inoculation. Mice were infected with either Spv+ 3456 or Spv UF110. Three days later, splenic CFU were examined. Mean with standard deviation of log10 splenic CFU for 3456 or UF110 are shown; n for each group is shown above the bars. In control-treated mice, 3456 was significantly higher than UF110 for splenic CFU (P < 0.05); however, in cyclophosphamide-liposome-dCMdP-treated mice, recovery of the Spv strain was insignificantly higher than the Spv+ strain (P > 0.05). Similar results were obtained for livers (data not shown). (B) Mixed-strain inoculation. Mice were infected with a mixture of Spv+ 3306 and Spv UF110. Two to three days later, CFU of each strain in spleens were examined. Mean log10 CFU with standard deviation for 3306, UF110, and the mean paired difference (Diff.) are shown. In control-treated mice, the mean paired difference between 3306 and UF110 was significantly greater than 0 (P < 0.05); however, in cyclophosphamide-liposome-dCMdP-treated mice the mean paired difference between 3306 and UF110 was not significantly greater than 0 (P > 0.5). Similar results were obtained for livers (data not shown). Numbers of mice in each group are shown above the bar for the mean paired difference.

Invasion of nonphagocytic host cells is not necessary for the functional expression of the Spv phenotype or systemic infection by S. typhimurium after s.c. inoculation. The experiments described above left open the possibility that Spv-mediated growth occurred within a nonphagocytic host cell, e.g., hepatocyte. In fact, Conlan and North (8) reported that S. typhimurium proliferates within hepatocytes of i.v.-inoculated mice. To examine if infection of nonphagocytic host cells was important in the Spv phenotype of increased intracellular growth rate, salmonellae were mutated so as to be defective for intracellular infection of nonphagocytes. This was accomplished by transduction with the invA::aphT mutation, which inhibits invasion into, but not adherence to, nonphagocytic cells (21). We confirmed that InvA Spv⁺ UF102 and InvA Spv UF103 were approximately 1,000-fold inhibited for invasion into Henle-407 cells and that the invasion defective genotype could be complemented in trans by plasmid pYA2217 encoding the S. typhimurium inv region (20) (data not shown). To examine if InvA salmonellae could still be phagocytosed by macrophages after opsonization with complement, which would most closely mimic the in vivo condition, J774.1 cells or peritoneal macrophages were infected with InvA⁺ and InvA S. typhimurium. We detected a slight difference in the abilities of the bacteria to adhere to and be taken up by macrophages in the absence of complement. However, opsonization with 10% normal rat serum more than compensated for any deficiency of InvA salmonellae to enter macrophages (data not shown).

View larger version (28K): [in this window] [in a new window]   FIG. 8.   Preventing invasion of nonphagocytes by the invA::aphT mutation does not significantly affect systemic infection by either Spv+ or Spv S. typhimurium after s.c. inoculation of mice. Mice were inoculated s.c. with Spv+/InvA+ 3306, Spv+/InvA UF102, Spv/InvA+ 3337, or Spv/InvA UF103. Five days later, splenic and hepatic CFU were enumerated. Data are shown as mean with standard deviation for log10 CFU/spleen or CFU/gram of liver. All groups contain four mice. Differences between 3306 and 3337 or UF102 and UF103 were significant (P < 0.02 to P < 0.005); however, differences between 3306 and UF102 or 3337 and UF103 were not significant (P > 0.1 to P > 0.5).

	DISCUSSION

Top Abstract Introduction Materials & Methods Results Discussion References

Molecular genetic analysis of the spv genes of the salmonella virulence plasmid has proceeded at a rapid pace (reviewed in references 25 and 29). Although a region of the virulence plasmid encoding five genes, spvRABCD, has been identified through cloning, mutagenesis, and DNA sequencing, the exact roles of most of these genes in virulence are not known (29). The SpvR protein is a positive regulator of spvABCD (29). Regulation of the spv genes is dependent on the alternative sigma factor RpoS (14, 48, 66), and the genes are expressed within host cells (16, 52, 66). We have determined that the spv genes do not require a cessation of bacterial growth for induction but instead can be greatly induced in minimal medium mimicking the intracellular environment of animal cells (66, 67).

The elucidation of the virulence plasmid phenotype has undergone significant evolution since the first description of the plasmid as being associated with virulence (36). Initial studies suggested that the plasmid of S. typhimurium was involved with adherence and invasion into tissue culture cells, serum resistance, and lipopolysaccharide biosynthesis; however, each of these hypotheses has not proven reproducible (29). Our own data demonstrated that plasmid-cured S. typhimurium was deficient in recovery from lymphoid tissues after oral inoculation of BALB/c mice but was capable of surviving in the lymph nodes and spleen for extended periods (28). These results were not consistent with the plasmid affecting resistance to complement or survival within phagocytes. Indeed, short-term survival rates of virulence plasmid-containing and cured salmonellae were equivalent in murine macrophages (28). Others have confirmed this result and extended in vitro infection of macrophages to as long as 24 h without demonstrable differences between wild-type and cured strains (54). In only a single study by Libby et al. (40) was a moderate divergence achieved in recoveries of plasmid-containing and cured S. dublin in cultured bovine macrophages. However, the difficulty in reproducing the Spv phenotype in vitro does not definitively disprove a role for the spv genes in these phenotypes in vivo. In vitro models may not sufficiently mimic the complexities of the host-pathogen interaction which occurs in vivo. We therefore concentrated our studies of the Spv phenotype by using the mouse model. By using genetic markers (temperature-sensitive antibiotic resistance plasmid pHSG422) and genetic manipulation (aroA), we previously demonstrated that the spv genes primarily increase the growth rate of S. typhimurium in BALB/c mice without significantly affecting either the killing or movement of the bacteria in the mice (30). The resistance to in vivo-administered gentamicin by both virulence plasmid-containing and cured S. typhimurium strongly suggested that both strains were located intracellularly within host cells. The equal yields of both strains from the extracellular environment of peritoneal chamber implants supported this conclusion. However, the relevant host cell(s) for either permitting the intracellular proliferation of Spv⁺ S. typhimurium or conversely suppressing the intracellular growth of the Spv derivative was not identified. Our study described here was executed to probe the roles of different host cells in the salmonella Spv-mediated phenotype of increased intracellular growth rate.

Lymphocytes are not involved with Spv-mediated virulence. Since T cells can affect the abilities of macrophages and other cells to control intracellular pathogens such as S. typhimurium (37) and since S. typhimurium has been shown to infect lymphocyte cell lines in vitro (62), we examined the effect of lymphocytes on the Spv phenotype in BALB/c mice by using homozygous nude BALB/c mice and SCID BALB/c mice. By infection of normal and lymphocyte-deficient mice with mixtures of Spv⁺ and Spv S. typhimurium, we observed no significant effect on recoveries of either bacterial strain from spleens of orally inoculated mice within 5 days of inoculation (Fig. 1). We also demonstrated that T cells and B cells do not have a significant role in the Spv phenotype of increased intracellular growth rate. This is not surprising since the Spv phenotype is exerted as soon as 3 days after oral inoculation (28), and T cells probably do not have time to be stimulated and have any effect at this early time of the disease process. Our results are most consistent with those of Weintraub et al. (64) and Guilloteau et al. (24). Weintraub et al. performed a comprehensive analysis of the roles of and T cells in both Ity^r and Ity^s mice which were genetically deficient in T-cell subsets (64). In Ity^s C57BL/6 mice, infection with wild-type S. dublin was unaffected within 4 days of oral inoculation even when - and -T cells were lacking. Guilloteau et al. found that splenic and hepatic infection of SCID CB-17/IcrCru mice after i.p. inoculation was unaffected compared with normal BALB/c mice until 11 days postinoculation. Others have used a variety of congenic mouse strains, reconstitution, and depletion procedures to show in Ity^r mice that T cells are not important in suppressing the initial net replication of S. typhimurium after i.v. inoculation (34, 42, 49). In contrast, Emoto et al. (13) reported that mice genetically deficient for T cells were more resistant to i.p. infection with S. choleraesuis. Mixter et al. (46) depleted BALB/c mice of T-cell subsets by using monoclonal antibodies to the and T-cell receptors and reported that depletion of T cells decreased the oral 50% lethal dose (LD₅₀) of S. enteritidis 10⁴-fold, whereas depletion of T cells decreased the LD₅₀ 300-fold. The oral LD₅₀ determinations using S. enteritidis involved infection for as long as 14 days. A protective role for T cells within a week of i.p. inoculation of BALB/c mice with S. choleraesuis was reported by Matsumoto et al. (44), who depleted mice by using a monoclonal antibody. We feel that most emphasis should be placed on the use of genetically deficient mice and that care should be taken in interpreting results obtained by depleting mice of cell populations with antibodies. Phagocytes such as macrophages could become occupied clearing lysed host cells in tissues and thereby be less available for inhibiting the salmonella infection.

Macrophages are essential for Spv-mediated virulence. We next examined the roles of phagocytes in selecting against the growth of Spv S. typhimurium. We envisioned that either phagocytes were the permissive cell type for Spv-mediated intracellular growth or these cells suppressed Spv salmonellae within nonphagocytic cells. The potential roles of phagocytes in Spv-mediated virulence were examined by depletion of phagocyte populations from mice. At necropsy, cyclophosphamide treatment depleted circulating PMNs by more than 90%, depleted spleens and livers of PMNs, but did not decrease tissue macrophages. However, PMN depletion had no significant effect on the differential recovery of Spv⁺ S. typhimurium compared with Spv S. typhimurium from the spleens and livers of orally inoculated mice within 5 days of inoculation (Fig. 3). Between two sets of experiments performed months apart, we obtained different results on the effects of granulocytopenia on recovery of salmonellae from spleens and livers of orally inoculated mice. In the first set of experiments, there was no effect of granulocytopenia on either Spv⁺ or Spv salmonellae; however, in the second set of experiments, there was a significant increase in CFU recovered from systemic sites for both strains. These results suggest that PMNs may have a role in suppressing systemic infection by S. typhimurium but are not involved with or necessary for the suppression of Spv salmonellae. At the present, we cannot explain the differences between the two sets of experiments. In addition to PMNs, cyclophosphamide treatment would be expected to affect other rapidly dividing cell populations, including elicited monocytes that are generated from bone marrow precursors. Additionally, it is possible that the intestinal mucosa of the mice reacted differently between the two sets of experiments and that in the latter set, salmonellae were able to invade into deeper tissues more easily. We did not examine for effects on other cell populations, other than demonstrating that macrophages were still present in spleens and livers of cyclophosphamide-treated mice. Alternatively, the time course of functional PMN depletion may have been different between the experiments, and in those experiments in which recovery of salmonellae was increased by granulocytopenia, the PMN depletion may have occurred more rapidly than in those in which no significant effects on systemic infection were observed. The results from the first set of granulocytopenic mice (Fig. 3A) differ from, while those from the second set (Fig. 3B) agree with, results of Conlan (6) and Conlan and North (7), who used the same monoclonal antibody that we used for immunocytochemistry, RB6-8C5, to eliminate PMNs from mice. In these studies, which involved i.v. inoculation of mice with salmonellae, significant increases in systemic infection by S. typhimurium were noted in PMN-depleted mice. As discussed below, results from others examining effects on systemic salmonella infection with total body irradiation of mice, which induced granulocytopenia (24, 33), were as mixed as our own data shown in Fig. 3. A more definitive analysis of the role of PMNs in salmonella infection to reconcile all of these observations will require more detailed analysis utilizing granulocytopenia induced by both chemical (e.g., cyclophosphamide) and other (e.g., antigranulocyte monoclonal antibody and irradiation) treatments.

Invasion of nonphagocytes is not involved with Spv-mediated virulence and systemic infection beyond the intestines. Since we could not deplete mice of nonphagocytic cells, as we did for phagocytes and lymphocytes, we denied the bacteria access to the intracellular location of nonphagocytes with a mutation known to inhibit invasion, invA::aphT (21). invA is the 5' gene of the invABC operon located in pathogenicity island I at centisome 63 on the S. typhimurium chromosome (19). A polar mutation in invA, which abolished the expression of downstream inv genes, severely impeded the invasive phenotype of virulent strains of S. typhimurium and caused a significant increase in the LD₅₀ when administered orally to BALB/c mice (20). Our intended use of an invA mutation required that InvA salmonellae not invade any nonphagocytes in vivo and that InvA salmonellae could still be phagocytosed by phagocytes. Inv S. typhimurium is unable to invade hepatocytes in vitro (19a). We (data not shown) and others (4, 47) have confirmed that Inv salmonellae are phagocytosed by macrophages in vitro in nearly normal levels. We therefore assume that InvA S. typhimurium used in our studies have access to the intracellular environment of murine macrophages in vivo after s.c. inoculation. In fact, we have confirmed this hypothesis by using immunofluorescence analysis of tissues from infected mice (31).