INTRODUCTION

Top Abstract Introduction Materials and Methods Results Discussion References

African trypanosomes are extracellular parasitic protozoa that can be transmitted by the bite of the tsetse fly. They are the causative agent of human sleeping sickness and the related cattle disease Nagana. To complete their life cycle in a mammalian host and to interact with the host immune system, they have developed a number of specific adaptations. The main parasitic mechanism involved in host immune system evasion is based on a continuous antigenic variation of the glycosylphosphatidylinositol-linked major surface protein variant surface glycoprotein (VSG). A dense layer of 10⁷ copies of identical VSG molecules forms a protective coat for the trypanosome, and a regular switch in the expression of the VSG variants prevents efficient antibody-mediated parasite elimination (6, 23, 38, 40).

Despite the existence of the VSG, other trypanosome components of a conserved nature are part of a pronounced interaction between the host and the parasite. In the present study, we demonstrate that during the course of infection, the presence of trypanosome heat shock protein 60 (HSP60) is able to cause a significant host humoral immune response with an autoimmune character.

HSPs are highly conserved molecules produced by both prokaryotic and eukaryotic cells. Their main role is to preserve cellular functions under a variety of stress conditions. In particular, for a number of parasites it has been demonstrated that induction of HSP60 could be linked to the changing environmental conditions during passage from the mammalian host to the insect vector (20). Members of the HSP60 family function as molecular chaperones. They form a group of proteins that play a major role in folding, unfolding, and translocation of polypeptides as well as the assembly and disassembly of protein complexes (15, 16). During several infectious diseases such as with Mycobacterium tuberculosis, Chlamydia trachomatis, Toxoplasma gondii, Trypanosoma cruzi, and Leishmania major, HSP60 molecules are involved in the induction of host immune responses (13, 15, 16, 26). Due to the conserved nature of the amino acid sequence, infection-induced responses to pathogen HSPs may result in a final host response with an anti-self character. Although in mammalian cells HSP molecules are usually located within the intracellular compartment, an increasing body of evidence shows that during a progressing infection HSP60-derived epitopes can be recognized by the host immune system when expressed on the surface of mammalian cells (13, 39). In general, since both host cells and pathogens respond to stress by producing elevated HSP levels, an immune system recognition of the surface-expressed HSP molecules may be one part of a more complex interplay between the host and the pathogen.

In Trypanosoma brucei, HSP60 is a 63.7-kDa protein expressed by both bloodstream and procyclic forms. It has 51.3 and 94.5% amino acid identity to the mammalian homologue P1 protein and Trypanosoma cruzi HSP60, respectively (5).

Apart from the VSG and HSP molecules, another distinct group of antigens present on the trypanosome surface consists of several members of invariant surface glycoproteins (ISGs) (14, 44). Their invariant nature makes them an interesting tool for serological analyses of the samples from the infected host. ISG70 is much less abundant than the VSG (5.1 × 10⁴ copies/cell) but is also distributed over the entire plasma membrane (14). In contrast to the VSG, ISG70 is not attached to the surface by a glycosylphosphatidylinositol anchor, so that the release of ISG70 is related to the elimination of trypanosomes during the infection (14).

In the present study, we analyzed a recognition of both trypanosome- and host-specific HSP60 peptides. This study showed that during the course of experimental T. brucei infections the induction of an anti-self humoral response takes place. Together with a recent report about the existence of autoreactive anti-VSG antibodies, these results pointed to the fact that autoimmune responses may play an important role in the interplay between the host and the parasite (21). Moreover, the profiles of immunoglobulin (Ig) isotype switching produced against HSP60, ISG70, and VSG were found to depend on both the antigen type and the stage of the infection.

	MATERIALS AND METHODS

Top Abstract Introduction Materials and Methods Results Discussion References

Mice and trypanosomes. Both the pleomorphic AnTat 1.1E clone from the EATRO 1125 stock of T. brucei and a derived monomorphic AnTat 1.1 clone were kindly provided by N. Van Meirvenne (Institute of Tropical Medicine, Antwerp, Belgium). Parasite stabilates were stored in liquid nitrogen. To obtain parasites for infection studies, a mouse was infected intraperitoneally with a stabilate volume containing 50,000 living parasites. On day 3 of the infection, blood was taken, supplemented with heparin (15 U/ml), and used for infection of experimental groups of mice. To monitor the course of the parasitemia, 6- to 8-week old female BALB/c mice and athymic BALB/c^nu/nu mice (Harlan) received an intraperitoneal injection of fresh blood, containing 5,000 parasites. At time intervals of 2 or 3 days, the number of parasites present in the blood was counted under a light microscope and an infectious serum sample was collected for the antibody titer analyses.

Preparation of trypanosome lysates and soluble VSG. Trypanosomes were harvested from infected blood by DE52 chromatography with sterile phosphate-buffered saline (PBS) (pH 8.0) supplemented with 1% glucose for equilibration and elution. After separation, the parasites were washed and resuspended in sterile PBS. Crude parasite lysate was obtained by three freeze-thaw cycles in the presence of 1 mM Pefablock protease inhibitor (Boehringer, Mannheim, Germany) and 0.01 mM E64 (Sigma Chemical Co., St. Louis, Mo.). Soluble VSG was prepared from DE52-purified parasites by osmotic lysis for 5 min at 37°C at 10⁹ cells/ml in 10 mM sodium phosphate (pH 8.0) containing 0.1 mM N-p-tosyl-L-lysine chloromethylketone (TLCK) and 0.1 mM phenylmethylsulfonyl fluoride (both from Boehringer). The supernatant was passed through a DE52 column equilibrated in 10 mM sodium phosphate (pH 8.0). Soluble VSG was further purified on a Sephacryl-S200 column (Pharmacia Biotech, Uppsala, Sweden), dialyzed against water overnight at 4°C, and freeze-dried. The protein concentration of the trypanosome lysates and VSG was estimated by using a detergent-compatible protein assay kit (Bio-Rad Laboratories, Hercules, Calif.) with bovine serum albumin (BSA) as a standard.

HSP60 synthesis. HSP60 peptides were synthesized with an Applied Biosystems model 431 A peptide synthesizer by Merrifield's solid-phase Fmoc/TBTU-HOBT method. The reaction was performed on a 0.25 mmol scale. The initial Cys amino acid was esterified (0.5 mmol/g) on a 4-hydroxymethylphenoxy polystyrene-1% DVB resin (Wang resin). The following side chain protecting groups were used: Asp, Glu, and Thr, t-butyl; Asn and Gln, trityl; Arg, 2,2,5,7,8-pentamethyl-chroman-6-sulfonyl (Pmc); Lys, t-butyloxycarbonyl. After synthesis, dried resin-bound peptides were dissolved in a solution of 40:2:2:1:3 (by vol) TAE-water-thioanisole-ethanedithiol-phenol (10 ml) and incubated with continuous shaking for 2 h at room temperature. The peptides were filtered, washed, and precipitated from solutions by diethyl ether addition and were dissolved in 10% acetic acid and lyophilized. They were purified by reverse-phase chromatography on a Vydac C₁₈ silica gel column (300-Å porosity,17-µm particle size; 25 by 25 mm) with a linear gradient solvent system. At the end of the procedure, peptides were additionally checked by both amino acid sequence analyses and by mass spectrometry. Their homogeneity was assessed by analytical high-pressure liquid chromatography (Pharmacia Biotech, Uppsala, Sweden). The peptides were found to be more than 98% pure.

Cloning and purification of T. brucei HSP60. The HSP60 cDNA was isolated from a gt11 library screening by using serum of infected mice, and the entire HSP60 open reading frame was subcloned into expression vector p-CAL-nk-EK (Stratagene). T. brucei HSP60 was expressed as a fusion protein with a calmodulin binding peptide, in accordance with the specifications supplied by the Stratagene protocol. After an enzymatic cleavage, an additional purification step was performed with a Sephacryl-S200 column (Pharmacia Biotech). The purity of the recombinant trypanosome HSP60 was verified by Western blot analysis and Coomassie blue staining. The protein concentration was measured by using the protein assay kit (Bio-Rad Laboratories) with BSA as a standard.

Quantification of HSP60 in the trypanosome lysate. A serial dilution (50 µl/well) of the purified standard HSP60 (30 down to 2.5 µg/ml) and 50 µl of a 1:10 dilution of trypanosome lysate (5 mg/ml) were blotted onto nitrocellulose filters by using a dot blot transfer apparatus. The filters were blocked for 1 h with a 1% skim milk-100 mM Tris-NaCl (pH 8.0) solution at room temperature. After multiple washes, the filters were incubated overnight at 4°C with rabbit anti-trypanosome HSP60 polyclonal antibodies (dilution, 1:500). The washed filters were incubated for 1 h at room temperature with a secondary anti-rabbit IgG antibody coupled to alkaline phosphatase (Promega) (dilution, 1:7,500). After several washes, the filters were stained for 15 min in a developing alkaline phosphatase solution (100 mM Tris-NaCl, 5 mM MgCl₂ [pH 9.5]) containing two substrates: nitroblue tetrazolium (NBT) (Promega) and 5-bromo-4-chloro-3-indolylphosphate (BCIP) (Promega). The trypanosome HSP60 was quantified with a GS 690 imaging densitometer apparatus (Bio-Rad Laboratories).

Detection of trypanosome HSP60 with infectious serum by using a dot blot technique. Purified trypanosome HSP60 (1 µg) was spotted onto nitrocellulose filters, which were blocked overnight in 1% skim milk-100mM Tris-NaCl (pH8.0) solution at 4°C. After multiple washes, a 1:100 dilution of serum samples from different time points during the infection was added in triplicate to the nitrocellulose filters and incubated overnight at 4°C. The filters were washed and incubated for 1 h at room temperature with a mixture of the secondary goat anti-mouse IgG (Promega) (dilution, 1:7,500) and IgM (Sigma Chemical Co.) (dilution, 1:10,000) polyclonal antibodies, both coupled to alkaline phosphatase. Finally, the filters were washed and developed for 15 min by addition of NBT and BCIP substrates in alkaline phosphatase developing buffer (pH9.5). The images were analyzed with a Bio-Rad GS690 densitometer.

Immunodetection of anti-HSP60, anti-VSG, and anti-ISG70 responses in ELISA. The titers and isotype pattern of the specific antibody response against total HSP60, HSP60-derived peptides, VSG, and ISG70 were determined by specific enzyme-linked immunosorbent assays (ELISAs). ELISA plates (Nunc) were coated overnight at 4°C with 5 µg of the trypanosome-derived antigens per ml. The plates were washed three times with PBS supplemented with 0.05% Tween 20 (Polylab Biochemicals) and further blocked for 1 h at room temperature with 1% skim milk solution in PBS. After three washes, serial dilutions of the individual infectious serum samples were added in triplicate to the plates and incubated for 1 h at room temperature. The washed plates were incubated for another 1 h at room temperature with a series of secondary antibodies: either anti-mouse IgG2a or IgG1 (Serotec) or anti-mouse IgM (Sigma Chemical Co.) coupled to alkaline phosphatase or anti-mouse IgG3 or IgG2b (Serotec) coupled to horseradish peroxidase. After 1 h of incubation, the plates were washed and incubated with the substrates, respectively 4-nitrophenylphosphate, disodium salt hexahydrate (NBT) (Acros Organics) for alkaline phosphatase and 3,3',5,5-tetramethylbenzidine (TMB) (Sigma Chemical Co.) for horseradish peroxidase. The alkaline phosphatase-containing plates were left to develop for 30 min, and the optical density was measured at 405 and 690 nm to subtract nonspecific absorption. The horseradish peroxidase-containing plates were left to develop for 15 min, and the reaction was stopped by addition of 1 M H₂SO₄. The optical density was measured at 450 and 690 nm.

Immunodetection of trypanosome antigens by infectious serum in Western blotting. The purified trypanosome HSP60 and VSG as well as total parasite lysates, were run under reducing conditions on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels (10% polyacrylamide) and blotted onto the nitrocellulose filters by electrotransfer for 1 h at 100 V. The filters were blocked overnight in 1% skim milk-100 mM Tris-NaCl (pH 8) solution at 4°C. The washed filters were incubated overnight at 4°C with a 1:200 dilution of serum collected during the chronic phase of infection. After the washings, secondary anti-mouse IgG (Promega) and anti-mouse IgM (Sigma Chemical Co.) polyclonal antibodies, both coupled to alkaline phosphatase, were added for a 1-h incubation at room temperature. After the last wash, the filters were developed by the addition of NBT and BCIP substrates in alkaline phosphatase buffer.

Electron microscopy. AnTat 1.1E bloodstream parasites were harvested from the blood of infected mice at the first peak of parasitemia. The parasites were fixed for 20 min in 3.5% paraformaldehyde-0.5% glutaraldehyde in PBS. After methanol dehydration, the parasites were embedded in Lowicryl polymerized under a UV light at 20°C. Ultrathin sections were collected on carbon-Formvar-coated copper grids and blocked in 10% normal goat serum (Sigma Chemical Co.) diluted in PBS solution containing 1% BSA. Rabbit anti-HSP60 polyclonal antibody (1:40 dilution in 1% BSA) was added for 1 h of incubation at room temperature. After three washes in PBS-BSA solution containing 0.05% Tween 20 (Polylab Biochemicals), secondary goat anti-rabbit IgG coupled to 15-nm gold particles (GAR15; British BioCell) (1:100 dilution in PBS-1% BSA) was added for another 1 h of incubation at room temperature. After three washes, sections were stained with uranyl acetate and lead citrate and images were observed in a AEI 6B electron microscope at 60 kV.

Immunofluorescence microscopy. The trypanosomes were resuspended at a density of 10⁶ cells/ml in PBS buffer and fixed by addition of 3.5% paraformaldehyde and 0.5% glutaraldehyde for 15 min at room temperature. They were permeabilized in 0.1% Triton X-100 (Sigma Chemical Co.) for another 15 min at room temperature. After two washes with PBS buffer, neutralizing 0.1 M glycine solution was added and the mixture was incubated for 15 min at room temperature. After three additional washes, 20 µl of parasite suspension was placed onto poly-L-lysine (1 mg/ml) (Sigma Chemical Co.)-coated coverslips and left to adhere for 30 min at room temperature. After several washes with PBS, the parasite-coated coverslips were blocked by the addition of 1% BSA-PBS solution for 1 h at room temperature. After repetitive washes, mouse anti-HSP60 antibody or rabbit anti-ATP synthase antibody, or both antibodies together, were added for an additional 1 h of incubation at room temperature. Anti-mitochondrial ATP synthase rabbit antibody from Crithidia fasciculata, which showed cross-reactivity with the ATP synthase  subunit of T. brucei, was a kind gift from R. Benne (Amsterdam) (24). After washes, the presence of ATP synthase was revealed with a secondary anti-rabbit Texas red-coupled antibody (Amersham) (1:100 dilution), while the HSP60 distribution was demonstrated by using an anti-mouse fluorescein isothiocyanate (FITC) (Amersham) (1:100 dilution)-coupled antibody for a 1-h incubation at room temperature. After a last wash, Vectashield mounting medium (Vector Laboratories) containing 100 ng of 4',6-diamino-2-phenylindole (DAPI) (Sigma Chemical Co.) was added to the parasite-containing coverslips. The images were recorded with a charge-coupled device camera connected to a Zeiss Axioscope microscope and were processed with ISIS 3 software.

Intracellular flow cytometry analysis. The trypanosomes were resuspended at a density of 10⁶ cells/ml in PBS buffer and fixed by addition of 3.5% paraformaldehyde and 0.5% glutaraldehyde for 15 min at room temperature. They were permeabilized in 0.1% Triton X-100 for another 15 min at room temperature. After two washes with PBS buffer, neutralizing 0.1 M glycine solution was added and the mixture was incubated for 15 min at room temperature. After two washes with PBS, parasite aliquots were incubated for 30 min on ice with rabbit polyclonal anti-HSP60. The samples were washed twice in PBS and further incubated for 30 min on ice with a secondary sheep anti-rabbit FITC-coupled antibody (ICN). The samples were washed twice in PBS and analyzed on a Ventage SI apparatus (Beckton Dickinson).

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

Quantification and cellular distribution of the HSP60 in T. brucei. HSPs are responsible for maintaining cellular functions under a variety of stress conditions related to a progressing infection. Besides their function as chaperones, they seem to be involved in induction of host immune response (15, 16). Here, HSP60 expression and anti-trypanosome HSP60 responses were analyzed during experimental trypanosome infections.

View larger version (18K): [in this window] [in a new window]   FIG. 1.   Kinetics of anti-HSP60 antibody responses during experimental pleomorphic infection of BALB/c mice with T. brucei AnTat 1.1E. Serum samples were isolated from infected mice on the days indicated, and a densitometric dot blot analysis was performed with 1 µg of purified trypanosome HSP60 per datum point. Density quantification is expressed as absorbense units per square millimeter. Values are presented as mean optical density (O.D.) of three measurements and standard deviation.

View larger version (35K): [in this window] [in a new window]   FIG. 2.   (a) Western blot analysis of T. brucei AnTat 1.1E soluble extracts immunostained with the infectious serum isolated from BALB/c mice during the chronic phase of pleomorphic infection. Lane 1 shows the molecular weight (MW) markers (in thousands), and lane 2 shows the background detection of a total trypanosome extract by the serum from non-infected mice. Lanes 3, 4, and 5 respectively contain total trypanosome extract, purified VSG, and purified trypanosome HSP60, stained with serum isolated from infected mice. (b) Quantification of the trypanosome HSP60 by the dot blot technique. A serial dilution of purified HSP60 (50 µl) was used to obtain a standard for densitometric analysis; 25 µg of total trypanosome extract was spotted per dot.

View larger version (23K): [in this window] [in a new window]   FIG. 3.   Intracellular flow cytometry analyses of HSP60 expression in pleomorphic and monomorphic trypanosomes. (a to e) Expression of HSP60 in pleomorphic AnTat 1.1E trypanosomes was measured on days 3 (a), 4 (b), 5 (c), 12 (d), and 35 (e) of an experimental T. brucei infection in BALB/c mice. (f) HSP60 expression in monomorphic AnTat 1.1 trypanosomes was analyzed on day 5 of infection.

View larger version (73K): [in this window] [in a new window]   FIG. 4.   Transmission electron microscopy and immunofluorescence microscopy analysis of the cellular localization of trypanosome HSP60. (a and b) Indirect immunogold labeling reveals the presence of HSP60 in the mitochondrion (m) and around the kinetoplast (k) (ma, maculae adherentes of the junction complex between the body and the flagellum). Bar, 0.5 µm. (c to e) Immunofluorescence shows the colocalization of HSP60 (FITC staining) (c and e) with the mitochondrial marker ATP synthase (Texas red staining) (d and e) in the mitochondrion. The DAPI blue fluorescence was used to indicate the nucleus (solid arrows) and the kinetoplast (arrowheads). Bar, 10 µm.

Humoral responses to the trypanosome HSP60. To determine specific titers and patterns of antibody isotypes recognizing the trypanosomal HSP60, serum was collected on days 4, 5, 6, 7, 10, 20, and 30 of pleomorphic infection and analyzed in an trypanosome HSP60-specific ELISA. As shown in Fig. 5a, trypanosome HSP60 was recognized as early as 4 days after infection. IgG2a antibody titers were predominant and reached peak values on days 5, 6, and 7, encompassing the whole first peak of parasitemia. The induction of IgG2a was accompanied by an IgG2b antibody response, however at lower magnitude. Therefore, both IgG2a and IgG2b antibodies induction was restricted to the first line of host immune response.

View larger version (17K): [in this window] [in a new window]   FIG. 5.   ELISA detection of anti-HSP60 antibody isotype responses during experimental pleomorphic infection of BALB/c mice with T. brucei AnTat 1.1E parasites. Serial dilutions of serum collected at various time points of infection were added to trypanosome HSP60-coated ELISA plates (5 µg/ml), with the starting dilution being 1/25. (a) Detection of IgG2a and IgG2b anti-HSP60 antibodies. (b) Detection of IgG3, IgG1, and IgM anti-HSP60 antibodies. The measurements were made with sera from five individual mice, and the values represent the means of specific end-point serum titers and standard deviation, with noninfected serum as a background detection limit.

View larger version (12K): [in this window] [in a new window]   FIG. 6.   ELISA detection of anti-HSP60 antibody isotype responses during experimental monomorphic infection of BALB/c mice with T. brucei AnTat 1.1E parasites. Serial dilutions of serum collected on day 5 postinfection were added to trypanosome HSP60-coated ELISA plates (5 µg/ml), with the starting dilution being 1/25. Detection of IgG2a, IgG2b, IgG3, IgG1, and IgM anti-HSP60 antibodies was performed. The measurements were made with sera from five individual mice, and the values represent the means of specific end-point serum titers and standard deviation, with noninfected serum as a background detection limit.

Anti-HSP60 responses in an athymic BALB/c^nu/nu mice. To be able to estimate the relative importance of the T-cell mediated response during experimental trypanosome infection, the T. brucei AnTat 1.1E parasitemia pattern in athymic BALB/c^nu/nu mice was analyzed. First, the results in Table 1 showed no major differences between the parasitemia in these mice and that in wild-type BALB/c mice. Both the levels of parasitemia and survival time were similar. As in the wild-type BALB/c-infected mice, HSP60 recognition was characterized by an early appearance of high IgG2a and IgG2b antibody titers (Fig. 7a). Although IgG2a antibody induction was predominant, both isotypes were induced equally fast and reached their maximum level at the first peak of the parasitemia. Thus, the early induction of IgG2a and IgG2b anti-HSP60 response does not require T-cell help and probably results from a short stumpy form-induced polyclonal B-cell activation. On the other hand, the results also suggest that the late-stage anti-HSP60 responses (IgG3, IgG1, and IgM) require T-cell help, since they were strongly reduced in athymic BALB/c^nu/nu mice (Fig. 7b).

View larger version (15K): [in this window] [in a new window]   FIG. 7.   ELISA detection of anti-HSP60 antibody isotype responses during experimental pleomorphic infection of athymic BALB/cnu/nu mice with T. brucei AnTat 1.1E parasites. The conditions are identical to those used in the experiment in Fig. 5.

Analyses of the autoimmune character of anti-HSP60 humoral responses. Trypanosome and host HSP60 have high sequence identity, suggesting the possible induction of self-recognition and autoantibody formation. This hypothesis was evaluated by monitoring the antibody response to trypanosome-derived and mouse-derived HSP60 peptides. By sequence alignment, two distinct regions with minimal homology (amino acids 152 to 171 and 368 to 387) were identified (Table 2). These trypanosome-specific peptides were used for the detection of an anti-HSP60 antibody response during the course of a T. brucei infection. The infection-related recognition of the trypanosome-specific peptides was compared to the recognition of host-specific HSP60 peptides from the same region of minimal homology. End-point antibody titers against two trypanosome-derived peptides and two host-derived peptides were determined at the level of both early-induced IgG2a response and late-induced IgG3 antibody response. IgG2a antibodies recognizing both trypanosome- and host-derived HSP60 peptides were induced with similar kinetics (Fig. 8). Moreover, the kinetics of peptide-specific IgG2a antibody induction were similar to the antibody induction pattern measured for the whole trypanosome HSP60 molecule (Fig. 5a). Further, elevated IgG3 isotype responses were also recorded by using both trypanosome- and host-specific HSP60 peptides. However, antibody titers against host-specific peptides were slightly reduced compared to titers detected against the specific trypanosome peptides (Fig. 9). Again, the kinetics of peptide-specific IgG3 antibody induction were similar to those of the antibody induction pattern recorded for the whole-trypanosome HSP60 molecule (Fig. 5b). In both cases, a significant increase in antibody titers in serum started to occur around day 10. 

View larger version (28K): [in this window] [in a new window]   FIG. 8.   ELISA detection of IgG2a anti-HSP60 antibody isotype responses during experimental pleomorphic infection of BALB/c mice with T. brucei AnTat 1.1E parasites. The screening was performed on synthetic trypanosome HSP60 peptides (pep 1, pep 2) (a) and synthetic mouse HSP60 peptides (pep 1, pep 2) (5 µg/ml) (b) with serial dilutions of serum collected at various time points of infection; the starting serum dilution was 1/25. Measurements were made with the sera collected from 5 individual mice, and the values represents the means of specific end-point serum titers and standard deviation, calculated with noninfected serum as a background detection limit.

View larger version (23K): [in this window] [in a new window]   FIG. 9.   ELISA detection of IgG3 anti-HSP60 antibody isotype responses during experimental pleomorphic infection of BALB/c mice with T. brucei AnTat 1.1E parasites. The conditions are identical to those used in the experiment in Fig. 8.

View larger version (20K): [in this window] [in a new window]   FIG. 10.   ELISA detection of IgG2a (a) and IgG3 (b) anti-HSP60 antibody isotype responses during experimental pleomorphic infection of athymic BALB/cnu/nu mice with T. brucei AnTat 1.1E parasites. The screening was performed with synthetic trypanosome HSP60 peptide (Tryp-pep1) and synthetic mouse HSP60 peptide (Mouse-pep1). Serial dilutions of serum collected at various time points of infection were tested; the starting serum dilution was 1/25. Measurements were made with the sera collected from five individual mice, and the values represent the means of specific end-point serum titers and standard deviation, calculated with noninfected serum as a background detection limit.

Comparison between the anti-HSP60 response and the responses to ISG70 and VSG. To compare the antibody isotype pattern observed against HSP60 with the isotype pattern of antibodies directed against an ISG, the anti-ISG70 response was analyzed during the T. brucei infection. Only IgG2a and IgM antibodies were induced, and both were maximal during the late phase of the infection. IgG2b, IgG3, and IgG1 anti-ISG70 antibody titers were detectable but remained low during the whole course of infection (Fig. 11). In athymic BALB/c^nu/nu mice, anti-ISG70 antibody titers remained minimal during the whole course of infection (results not shown). Therefore, in contrast to the anti-HSP60 humoral response, anti-ISG70 antibody induction requires the involvement of a specific T-cell compartment.

View larger version (14K): [in this window] [in a new window]   FIG. 11.   ELISA detection of anti-ISG70 antibody isotype responses during experimental pleomorphic infection of BALB/c mice with T. brucei AnTat 1.1E parasites. The screening was performed on trypanosome ISG70 (5 µg/ml). The conditions are identical to those used in the experiment in Fig. 5.

View larger version (18K): [in this window] [in a new window]   FIG. 12.   ELISA detection of anti-VSG antibody isotype responses during experimental pleomorphic infection of BALB/c mice with T. brucei AnTat 1.1E parasites. The screening was performed on purified trypanosome VSG (5 µg/ml). The conditions are identical to those used in the experiment in Fig. 5.

View larger version (12K): [in this window] [in a new window]   FIG. 13.   ELISA detection of anti-VSG antibody isotype responses during experimental pleomorphic infection of athymic BALB/cnu/nu mice with T. brucei AnTat 1.1E parasites. The screening was performed on purified trypanosome VSG (5 µg/ml). The conditions are identical to those used in the experiment in Fig. 5.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

Experimental T. brucei infections in BALB/c mice are characterized by the appearance of several peaks of parasitemia followed by repeated elimination of parasites in the liver and lymphoid tissue of the host (1, 41). It has been proposed that antibody-mediated killing of parasites by macrophages (3, 9, 17), spontaneous short stumpy form trypanosome degradation (4, 31), cytokine-induced toxicity (18), and direct NO action (12) are the main effector mechanisms that finally lead to the typical repeating pattern of infection. Continuous antigenic variation of the VSG is obviously a major mechanism involved in this process, since it allows an efficient evasion from host immune responses. However, the VSG cannot be considered the unique parasite component crucial for the interplay with the host, since other trypanosome molecules appear to be involved in the induction of host cytokines and the development of immunosuppression (8, 37). Despite a general downregulation of both interleukin-2 (IL-2) and its receptor expression, it would nevertheless appear that the host maintains a specific T-cell-dependent antibody response throughout the whole course of infection (8, 29, 30, 36). In this study we characterized the induction of a humoral response against the trypanosome HSP60, a molecule with a high degree of sequence identity to that of the host, and compared this response with those induced against two other parasite antigens, ISG70 and variant VSG. We concluded that the three antigens lead to specific and independent profiles of immunoglobulin isotype switching including both T-cell-dependent and T-cell-independent mechanisms.

During the course of a T. brucei infection, the host immune system is confronted with the presence of parasite-derived HSP60, due to the regular trypanosome elimination during the progressing disease. The results presented here have shown that HSP60 expression is significantly upregulated in the spontaneously degenerating short stumpy form trypanosomes. This event might be an evolutionary parasite adaptation to be able to cope with sudden changes in environmental stress during the passage from the mammalian host to the insect vector.

During pleomorphic trypanosome infections in BALB/c mice, a high induction of antibodies recognizing trypanosome HSP60 occurred as rapidly as the induction of antibodies against the VSG surface coat. In contrast, anti-HSP60 antibody responses were minimal in monomorphic infections, where no parasite differentiation from long slender forms to short stumpy forms takes place. In wild-type BALB/c mice, the antibody response against HSP60 occurred in two distinct phases. During the first days of infection, HSP60 recognition was marked by the appearance of a high IgG2a antibody titer, accompanied by moderate IgG2b levels. In contrast, during the late phase of infection, it was limited to a clear induction of IgG3 and IgG1 isotypes. IgM response occurred throughout the whole infection, although its maximal level was recorded just before the end of the parasitemia. It is important to mention here that, as demonstrated before (22), serum from uninfected mice contains significant levels of IgM antibodies that recognize trypanosome components. In the context of trypanosome HSP60 recognition, our results indicate that at the beginning of a trypanosome infection, the difference between the levels of these IgM antibodies and infection-induced IgM antibodies is very low. At the end of the infection, however, a clear increase in IgM antibody titers was observed. In athymic BALB/c^nu/nu mice, the humoral response to HSP60 during the first peak of infection consisted mainly of IgG2a and IgG2b. Only low levels of IgM, IgG1, and IgG3 antibodies were observed in the late phase. Therefore, a T-cell-independent recognition of HSP60 was dominant during the early phase of parasitemia and a T-cell-dependent response was mounted afterward.

Since trypanosome HSP60 shows homology to its host counterpart, HSP60 recognition was analyzed in the context of a possible anti-host HSP60 autoimmune recognition. The use of both trypanosome- and host-specific HSP60 peptides allowed us to confirm this hypothesis, since a clear infection-induced production of both IgG2a and IgG3 antibodies against these molecules could be demonstrated. Similar to the responses to the complete trypanosome HSP60, IgG2a anti-trypanosome and anti-host peptide recognition was associated with the early stage of the infection and was generally T-cell independent. The presence of the spontaneously degenerating short stumpy population is crucial for this response, since monomorphic parasite infections failed to induce it. Moreover, the response was shown to be antigen specific, since the kinetics of anti-HSP60 antibody induction was clearly distinct from the kinetics of induction of antibodies against the other parasite molecules tested. In contrast to the observed IgG2a response, the IgG3 anti-HSP60 response was associated with the late stage of infection and was T-cell dependent. This was observed at the level of the responses generated to the whole trypanosome molecule, as well as at the level of responses to both trypanosome-derived and host-derived HSP60 peptides. As such, the results indicate a mixed nature of both anti-trypanosome and anti-host antibody responses.

Although HSP60 molecules are usually not present on the cellular surface, an increasing body of evidence shows that during parasitic infection, host macrophages are able to express HSP60-derived epitopes on their surface when stressed by high gamma interferon (IFN-) concentrations (13, 35). In this case, host cells expressing HSP60-derived epitopes can become a target for CD8⁺ subsets of T cells, leading to autolysis (28, 35). Since a rapid induction of IFN- production is one of the features accompanying T. brucei infection (8), the appearance of self-reactive anti-HSP60 antibodies could possibly lead to the recognition and lysis of HSP60-expressing macrophages during the progressing infection.

Given that in other parasitic infections members of the HSP70 and HSP90 families are also able to induce a significant autoimmune response, we do not exclude the possibility that T. brucei-related anti-HSP60 antibody induction is part of a larger array of an anti-HSP recognition. In other parasitic infections, the induction of anti-HSP antibodies has been associated with the occurrence of autoimmune disorders accompanied by HSP-related pathology (15, 16, 43). In this context, it has even been shown that the induction of anti-VSG antibody responses originates partly from a self-reactive compartment (21, 23). Moreover, trypanosomiasis-related induction of autoantibodies was noted for other host-derived components such as DNA (7), cytoskeleton (22), neurofilament-associated proteins (2), calmodulin (27), and thyroglobulin (7).

In contrast to the high anti-HSP60 antibody levels, humoral responses to the specific ISG70 were weaker. Only low levels of IgG2a and IgM were detected, and the levels reached a maximum toward the end of pleomorphic infection. The low anti-ISG70 antibody titers could be explained by the marked predominance of antibody response against the VSG and HSP60 rather than by the limited immunogenicity of the ISG70 protein itself, since mice or rabbits immunized with ISG70 alone were able to generate specific antibodies (14). The minimal anti-ISG70 antibody induction obtained in athymic BALB/c^nu/nu mice suggests that the immune response to this antigen requires the involvement of T cells. It is possible that the mechanisms involved in the differential induction of high polyclonal antibody induction against homologous determinants and the low, T-cell-restricted antibody response to trypanosome-specific determinants evolved as a part of the trypanosome immune escape mechanism.

To gain a more general insight into the pattern of trypanosome-induced antibody isotypes, the anti-VSG immunoglobulin isotypes were analyzed as well. The experimental pleomorphic infection of BALB/c mice with T. brucei AnTat 1.1E gave rise to the simultaneous appearance of IgG2a, IgG2b, IgG3, IgG1, and IgM antibodies, which reached maximal titers when the parasite load fell below the detection limit just after the first peak of infection. IgG2a, IgG3, and IgG2b antibody production was predominant, while IgG1 and IgM responses were induced at lower levels. These patterns were similar in general to the previously described anti-VSG responses in T. rhodesiense-infected C57BL/6 mice (30). However, a marked difference occurred in the magnitudes of anti-VSG responses, which appeared to be significantly higher in the T. brucei BALB/c infection model (30). This difference may be due to an enhanced capability of BALB/c mice to maintain a polyclonal B-cell antibody response. Another difference was observed in athymic mice. While infection of BALB/c^nu/nu mice gave rise to a pronounced IgG2a response and a low IgG3 response, infection in C57BL/6^nu/nu mice led to elevated IgG3 and IgM antibody titers (30). Thus, VSG triggers both T-cell-dependent and T-cell-independent antibody responses, with isotype patterns that may depend upon the mouse strain used and the parasite model studied (25).

In in vitro B-cell activation assays, as well as during a number of parasitic infections, the local cytokine environment appears to determine the nature of immunoglobulin isotypes (10, 11, 33, 42). While IFN- seems to be responsible for a combined induction of the IgG2a and IgG3 antibodies, IL-4 may trigger the switching IgE and IgG1, whereas transforming growth factor  may be involved in the induction of IgA and IgG2b (33). Since T cells are considered to play a crucial role in isotype switching, a link is often made between Th1/Th2 cytokine patterns and the appearance of isotype profiles. However, cytokines themselves are insufficient to trigger an Ig switch in resting B cells. An additional stimulation is required by a B-cell activator, an antigen receptor cross-linker, or an activated T cell. In the absence of T cells, immunoglobulin isotype switching events can occur as well. For instance in athymic mice, NK cells can be the source of IFN-, while IL-4 can be secreted by the activated mast cells and transforming growth factor  production can be linked to macrophage and B-cell stimulation (32-34). During experimental T. brucei infections in BALB/c mice, the observed antibody isotype pattern was complex and could not be easily related to a distinct Th1 or Th2 T-cell response, especially when circulating antibody titers against different trypanosomal antigens were determined. For example, when trypanosome-induced anti-VSG responses were analyzed, both IgG2a and IgG3 isotype antibodies were detected throughout the course of the infection. However, when anti-HSP60 responses were studied, IgG2a isotype induction was observed in the absence of an IgG3 response, although both isotypes are considered to occur due to a IFN--driven response. Moreover, the anti-ISG70 responses consisted mainly of IgG2a isotypes, and IgG3 was not detectable. Along these lines, it is relevant to add that although an early IgG2b isotype response to HSP60 was found to be mainly T-cell independent, the early IgG2b anti-VSG response was induced in a T-cell-dependent manner. Finally, although most isotype patterns observed would suggest an overall Th1-like antibody response, the induction of early-stage anti-VSG IgG1 antibodies as well as late-stage anti-HSP60 IgG1 antibodies should be considered to be IL-4 mediated, confirming the complexity of the humoral anti-trypanosome response. Therefore, it would appear that during the course of a T. brucei infection, several switch factors and B-cell activation pathways can determine the humoral response. One of the possible explanations for this observation is that these responses result from mixed anti-parasite and anti-host immune reactions.