INTRODUCTION

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Haemophilus ducreyi is a gram-negative bacterium which causes chancroid, a sexually transmitted disease (STD). Although this infection is uncommon in the United States, it is a major cause of genital ulcer disease in developing countries worldwide (40). Recently, it was reported that ulcerative sexually transmitted diseases, such as chancroid, serve as cofactors for human immunodeficiency virus (HIV) transmission, inreasing the risk of acquiring HIV infection two- to fivefold (11). Histological analyses of genital ulcers resulting from H. ducreyi infection have increased numbers of CD4⁺ lymphocytes and therefore may increase a person's susceptibility to HIV infection (39). Another disturbing fact regarding H. ducreyi infections is the emergence of antibiotic-resistant strains in areas where this disease is more prevalent (22, 40). These factors have stimulated increasing research efforts designed to understand the mechanisms and virulence factors involved in the pathogenesis of chancroid.

Although little is known about the bacterial components of H. ducreyi that contribute to colonization and subsequent ulcer formation, several putative virulence factors have been described. Two cytotoxins, a hemolysin with cytotoxic activity and a diffusible cytotoxin, were shown to be toxic to human foreskin fibroblasts and epithelial cells in vitro (2, 12, 32-34). Also, unique pili have been described which may function in attachment (38). Another putative virulence factor is the lipooligosaccharide (LOS) molecule, which has been a primary focus of our research (29). Structural analysis has demonstrated that the principal LOS glycoform of H. ducreyi shares common epitopes with the LOS of other mucosal pathogens, such as Neisseria gonorrhoeae, Neisseria meningitidis, and Haemophilus influenzae (9, 29). More importantly, these LOS epitopes have been implicated as important virulence factors for these latter human pathogens.

There are several lines of evidence which suggest that H. ducreyi LOS plays a role in the pathogenesis of chancroid. Our previous data demonstrated that injection of purified LOS causes intradermal inflammation in experimental animal models (10). Also, H. ducreyi mutants expressing truncated LOS molecules exhibit reduced virulence in the temperature-dependent rabbit model of infection (4, 5). Zaretzky and Kawula reported that purified LOS induced interleukin-8 expression from HaCaT cells in vitro, which could stimulate an inflammatory response that may indirectly lead to lesion formation (45). Alfa and DeGagne showed that a high concentration of pure LOS could inhibit H. ducreyi adherence to human foreskin fibroblasts in vitro (1). In addition, a Tn916 H. ducreyi mutant, with a disruption in a D-glycero-D-manno-heptose heptosyltransferase gene, exhibited reduced adherence and invasion of human keratinocytes in vitro (18).

In this study, we used pyocin lysis to initially identify LOS mutants of H. ducreyi 35000. We have previously shown that this method can select organisms that express truncated LOS molecules (8), suggesting that these bacteria contain defects in the biosynthetic pathway or assembly of this principal glycolipid. One LOS mutant, termed HD35000R, was isolated and used to clone and sequence two separate genes involved in LOS biosynthesis. The first gene codes for a protein which has homology to heptosyltransferase III of Escherichia coli (20, 44), while the second gene codes for a protein with homology to 1,4-glucosyltransferase of N. meningitidis (24). Individual isogenic mutants with disruptions in the glucosyltransferase, the heptosyltransferase, and both glycosyltransferase genes were constructed, and structures of the resulting LOS glycoforms were determined. Restoration of the wild-type LOS was accomplished by providing the wild-type H. ducreyi genes in trans in each isogenic mutant. These mutants will be important tools in providing a better understanding of the regulation and assembly of H. ducreyi LOS, which may lead to insight into the role of this molecule in pathogenesis.

	MATERIALS AND METHODS

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Bacterial strains and culture conditions. The bacterial strains used in this study are listed in Table 1. E. coli strains were grown at 37°C on Luria-Bertani (LB) agar plates or in LB broth. When needed, LB medium was supplemented with kanamycin, ampicillin, or chloramphenicol at a final concentration of 20, 50, or 30 µg/ml, respectively. H. ducreyi strains were cultured at 35°C in 5% CO₂ on chocolate agar plates or in brain heart infusion broth as previously described (10). When needed, chocolate agar plates were supplemented with kanamycin (20 µg/ml), chloramphenicol (1 µg/ml), and/or 5-bromo-4-chloro-3-indolyl--D-galactopyranoside (X-Gal; 40 µg/ml). Pseudomonas aeruginosa strain C was grown in Pseudomonas broth (14).

Pyocin isolation. Pyocin was isolated from cultures of P. aeruginosa strain C by the method described by Morse et al. (31).

Pyocin lysis assay. The pyocin selection assay was performed as described previously (8, 14). Clones resistant to lysis were tested for loss of reactivity with monoclonal antibody (MAb) 3F11. This antibody reacts to the terminal Gal-GlcNAc moiety conserved on the LOS of most gonococcal strains and also reacts with the LOS of most H. ducreyi strains (9, 28).

Complementation of HD35000R. A plasmid library of H. ducreyi chromosomal DNA was previously constructed in the pLS88 shuttle vector which had been modified by the addition of NotI restriction sites (39a). This plasmid library was electroporated into HD35000R by a previously described procedure (6). Transformants were selected on chocolate agar containing kanamycin (20 µg/ml) and immunoscreened by probing nitrocellulose lifts with MAb 3F11.

Recombinant DNA techniques. Plasmid isolations were performed using Qiagen purification kits (Qiagen, Chatsworth, Calif.). Restriction enzymes were purchased from New England Biolabs Inc., Beverly, Mass. T4 DNA ligase was purchased from Promega (Madison, Wis.). Restriction enzyme digests, ligations, transformations, and electroporation of E. coli were performed using standard methods (37). Electroporation of H. ducreyi was performed as previously described (6). PCR was used to produce double-stranded DNA probes and evaluate isogenic mutants. Approximately 100 ng of chromosomal DNA was used for the template. Annealing temperatures varied with primers used. Taq DNA polymerase was purchased from Fisher Scientific (Pittsburgh, Pa.).

Nucleotide sequence analysis. Both strands of the 3.2-kb NotI insert of pLS88.8 were sequenced. Sequence analysis of the cloned genes was performed using MacVector 6.0 and the Wisconsin sequence analysis packages (Genetics Computer Group, Madison, Wis.).

Construction of isogenic mutants. To mutate the H. ducreyi waaQ gene located on pLS88.8, pUCECAT was digested with EcoRI to remove the cat cartridge, treated with Klenow fragment, and ligated with pLS88.8, which had been digested with XbaI, and treated with Klenow fragment. To mutate the lgtF gene on pLS88.8, the cat cartridge from pUCCAT was isolated as a BamHI fragment and ligated to pLS88.8 which had been digested with BglII. For construction of mutations in both the waaQ and lgtF genes, pLS88.8 was digested with XbaI and BglII, liberating a 1.3-kb fragment containing portions of the waaQ and lgtF genes. The digested plasmid was then treated with Klenow fragment and ligated to the cat cartridge, which had also been blunt ended. Ligation mixtures were used to electroporate E. coli XL1-Blue, and transformants containing the cat cartridge in derivatives of pLS88.8 were selected on chloramphenicol and kanamycin. Plasmids with the correct restriction map were saved as pLS88.8CAThep, pLS88.8CATglu, and pLS88.8CAThepglu. Isogenic mutants of H. ducreyi 35000 were constructed as previously described by Bozue et al. (6). The NotI fragments from pLS88.8CAThep, pLS88.8CATglu, and pLS88.8CAThepglu were individually cloned into pRSM2072 (a derivative of pRSM1791 with an improved cloning site (L. Taratino and R. S. Munson, Jr., unpublished data) which had been digested with NotI. After transformation into E. coli, plasmids with the correct restriction map were saved as pMJFhep, pMJFglu, and pMJFhepglu. pMJFhep, pMJFglu, and pMJFhepglu were electroporated into H. ducreyi 35000, and cointegrates were selected on chocolate agar containing chloramphenicol. Isogenic mutants were selected by their ability to grow normally (large white colonies) on chocolate agar containing chloramphenicol and X-Gal. The isogenic mutants were designated 35000hep, 35000glu, and 35000hepglu.

Southern blot analysis. Chromosomal DNA was isolated from H. ducreyi strains using a modification of a previously described procedure (36). H. ducreyi strains were grown overnight on chocolate agar. Bacteria were harvested and resuspended in brain heart infusion broth, and chromosomal DNA was isolated. Chromosomal DNA was then digested to completion with HindIII or NcoI, electrophoresed on a 0.8% agarose gel, and then transferred to Immobilon-Ny+ membranes (Millipore, Bedford, Mass.) by capillary blotting overnight. Probes for the waaQ, lgtF, and cat genes were generated by PCR with the following primers. Primers P1 (5'-GATGCCTGTTGAGCCTCAGATTC-3') and P2 (5'-TTGTTTACCGCTAGGGGGACAG-3') were used to amplify a 505-bp internal probe to the H. ducreyi waaQ gene. A 571-bp internal probe to the lgtF gene was generated using primers P3 (5'-ACTCCGTGTACGATCCCATAAGTC-3') and P4 (5'-TGGTCCACAGAGACAATTTGCTC-3'). To analyze 35000hep-glu, a 320-bp probe to a region upstream of waaQ was prepared by PCR using primers P5 (5'-TCCAACGATAATGAAAAAACTGCTC-3') and P6 (5'-GATAGCGAATACCACTTTGCCAAG-3'). A 550-bp probe to the cat gene was also used in the Southern blot analysis. The templates for generation of the probes were pLS88.8 and p1710. DNA probes were biotinylated using a NEBlot Phototope labeling kit (New England Biolabs) as recommended by the manufacturer. Hybridizations were performed overnight at 64°C in 50 ng of denatured biotinylated probe per ml of 6× SSC-5× Denhardt's reagent, 0.5% sodium dodecyl sulfate (SDS) (20× SSC is 3 M NaCl plus 0.3 M sodium citrate [pH 7.0]). Washes were performed according to the NEBlot Phototope kit instruction manual. Detection was performed with a Phototope-Star detection kit (New England Biolabs).

Complementation of H. ducreyi waaQ and lgtF mutants. pLS88.8 was digested with both MunI and EcoRI to remove the entire waaQ gene. This digestion was subjected to agarose gel electrophoresis followed by purification with a GeneClean II kit (Bio 101 Inc., La Jolla, Calif.). The 1.2-kb DNA fragment containing the waaQ gene was ligated to the pLS88 vector which had been previously digested with EcoRI. To construct a plasmid containing the lgtF gene, pLS88.8 was used as a template in a PCR to amplify a DNA fragment containing the lgtF gene, using primers P7 (5'-TAAAGGTGAACGGGAACGAGCG-3') and P8 (5'-AATAGCACAAAAGGGGCGG-3'). The PCR product (1.2 kb) was then cloned into the pCR2.1 vector (Invitrogen). This fragment was excised by digestion with EcoRI, subjected to agarose gel electrophoresis followed by purification with a GeneClean II kit, and then ligated to EcoRI-digested pLS88. To construct a plasmid containing the waaQ and lgtF genes, pLS88.8 was digested with both MlsI and Eco1471 to remove open reading frames 3 and 4 (ORF3 and ORF4). This digestion was subjected to agarose gel electrophoresis followed by purification with a GeneClean II kit. The 6.7-kb DNA fragment containing the pLS88 vector and the two transferase genes was religated. Ligation mixtures were used to transform E. coli XL1-Blue. Plasmids were purified (Qiagen), and restriction analyses were performed to verify the constructs. The plasmids, pHEP, pGLU, and pHEPGLU, were used to electroporate 35000hep, 35000glu, and 35000hepglu, respectively. Selection of complemented H. ducreyi mutants was accomplished using chocolate agar containing kanamycin.

Preparation and analysis of H. ducreyi LOS. LOS from proteinase K-treated whole-cell lysates was resolved by SDS-polyacrylamide gel electrophoresis (PAGE) on a 14% acrylamide gel and visualized by silver staining (10, 41). Western blot analysis was performed by transferring LOS to a polyvinylidene difluoride membrane (Millipore, Bedford, Mass.) using a previously described procedure (26). LOS for structural analysis was extracted by the modified hot phenol-water procedure from bacteria that were grown overnight in 1600 ml of broth and dried (3, 23, 43).

Analysis of outer membranes proteins. Outer membrane proteins of H. ducreyi 35000 and the isogenic mutants were prepared by a previously described method (15, 25). Proteins were resolved by SDS-PAGE and stained with Coomassie blue (25).

Mass spectrometric analysis of H. ducreyi LOS. LOS structures from H. ducreyi strains 35000 and the three knockout mutants 35000hep, 35000glu, and 35000hepglu, along with the corresponding complemented strains, were analyzed by mass spectrometry. In each case, approximately 0.1 to 1.0 mg of LOS was converted to the corresponding water-soluble O-deacylated LOS glycoforms by treatment with hydrazine (37°C, 30 min) (21). Samples were then analyzed by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) using a PE Biosystems (Framingham, Mass.) Voyager DE time-of-flight mass spectrometer or a Voyager DESTR time-of-flight mass spectrometer as previously described (17). Both instruments were operated with a nitrogen laser (337 nm) in the negative-ion mode under delayed extraction conditions (42); delay time was 100 to 175 ns, and grid voltage was 92 to 94% of full acceleration voltage (20 to 30 kV). Samples were purified and desalted by drop dialysis using a 0.025-µm-diameter nitrocellulose membrane and/or by anion-exchange Zip Tips_AX (Millipore). Approximately 0.1 to 0.2 µg of O-deacylated LOS was mixed with 1 µl of a 320 mM 2,5-dihydroxybenzoic acid in 4:1 acetone-water (vol/vol) containing 175 mM 1-hydroxyisoquinoline (30), desalted with cation-exchange resin beads (DOWEX, 50×, NH₄⁺) and then air dried on a stainless steel target. Spectra were acquired and averaged (typically 20 to 50 laser shots), and mass was calibrated with an external calibrant consisting of an equimolar mixture of angiotensin II, bradykinin, luteinizing hormone-releasing hormone, bombesin, -melanocyte-stimulating hormone (CZE mixture; Bio-Rad) and ACTH 1-24 (Sigma, St. Louis, Mo.).

Nucleotide sequence accession number. The nucleotide sequence reported in this paper was deposited with GenBank and assigned accession no. AF215936.

	RESULTS

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Identification of a LOS mutant using pyocin selection. To identify genes involved in LOS biosynthesis, pyocin C was used to select for LOS mutants of H. ducreyi 35000 as described previously (8, 14). A single resistant colony was isolated and named HD35000R. Colony lift assay showed that this isolate did not react to MAb 3F11 (data not shown). MAb 3F11, developed to the LOS of N. gonorrhoeae strain 1291, reacts to an LOS epitope containing a terminal N-acetyllactosamine moiety conserved on over 90% of the H. ducreyi strains tested (9, 28). LOS from HD35000R was compared to the wild-type LOS by SDS-PAGE and Western blot analysis. The LOS from HD35000R (Fig. 1A, lane 2) migrated as a single band with a more rapid mobility than the LOS glycoforms produced by the parental strain (Fig. 1A, lane 1), which is indicative of a truncated LOS molecule. Western blot analysis demonstrated that the LOS of HD35000R lost reactivity to MAb 3F11 (Fig. 1B, lane 2), suggesting this structure lacks all or part of the terminal N-acetyllactosamine.

View larger version (47K): [in this window] [in a new window]   FIG. 1.   Composite of a silver-stained SDS-polyacrylamide gel (A) and the corresponding Western blot (B). The gel contains LOS from H. ducreyi 35000 (lane 1), HD35000R (lane 2), and the complemented pyocin mutant, HD35000R(pLS88.8) (lane 3). The blot was probed with MAb 3F11, demonstrating reactivity to wild-type LOS (lane 1) and with two glycoforms expressed by the complemented mutant HD35000R(pLS88.8) (lane 3). The LOS glycoforms that react with the MAb are marked with arrows.

Complementation of H. ducreyi 35000R. A plasmid library of H. ducreyi 35000 DNA, constructed in a derivative of the shuttle vector pLS88, was electroporated into HD35000R (Sun et al., submitted). Kanamycin-resistant transformants were screened for reactivity to MAb 3F11. Plasmid DNA was isolated from a positive transformant and electroporated back into HD35000R to confirm that this plasmid was responsible for the phenotype. The LOS from the complemented mutant, HD35000R(pLS88.8), was analyzed by SDS-PAGE and Western blotting. Figure 1A shows that the LOS isolated from HD35000R(pLS88.8) (Fig. 1A, lane 3) had an SDS-PAGE profile similar to that of the LOS of H. ducreyi 35000 (lane 1). However, Fig. 1A also shows that the complemented strain synthesized other larger glycoforms (lane 3) that were not readily apparent in the wild-type LOS (lane 1). Figure 1B shows that the LOS of HD35000R (pLS88.8) (lane 3) has reacquired the epitope recognized by MAb 3F11, indicating that the gene(s) present in the insert of plasmid pLS88.8 is sufficient to complement the LOS defect. Unexpectedly, MAb 3F11 also reacted with a slower-migrating LOS glycoform (Fig. 1B, lane 3) which appeared to contain di-N-acetyllactosamine, based on subsequent MALDI-MS analysis presented below.

Nucleotide sequence analysis of pLS88.8. The 3.2-kb DNA insert in pLS88.8 was sequenced and found to contain four complete ORFs (Fig. 2). ORF1 encodes a polypeptide of 344 amino acids, with a predicted molecular mass of 38.5 kDa, and shares 20% identity and 37% similarity to the waaQ gene product of E. coli (20, 44). The E. coli waaQ gene encodes heptosyltransferase III. On the basis of sequence homologies and LOS structural analysis (see below), we conclude that ORF1 encodes a heptosyltransferase III which adds the third heptose of the triheptose core of H. ducreyi 35000 LOS.

View larger version (6K): [in this window] [in a new window]   FIG. 2.   ORF map of the sequenced portion of pLS88.8. The 3.2-kb DNA insert was found to contain four ORFs. ORF1 is the putative heptosyltransferase III (waaQ); ORF2 is the putative glucosyltransferase (lgtF); ORF3 has homology to hypothetical protein HI1333 of H. influenzae; ORF 4 shares homology with a phosphatidylglycerophosphate phosphatase B (pgpB) of E. coli. Arrows represent the direction of transcription.

Construction of isogenic mutants. Isogenic mutants were constructed to confirm that the genes we identified encode the proteins responsible for each of the proposed functions. The putative H. ducreyi waaQ and lgtF homologues were independently inactivated by insertion of a cat cartridge into a unique restriction site within each of the genes. In addition, both genes were simultaneously inactivated by removal of a 1.3-kb DNA fragment containing portions of the heptosyl- and glucosyltransferase genes followed by insertion of the cat cartridge into this region. The resultant plasmids bearing insertions into waaQ (pLS88.8CAThep), lgtF (pLS88.8CATglu), and both genes (pLS88.8CAThepglu) were each digested with NotI, and the 4.4-kb (4.3 kb for the double mutant) DNA fragments containing the inactivated genes were then ligated into NotI-digested pRSM2072. Each construct was individually electroporated into H. ducreyi 35000, and clones were selected on chocolate agar supplemented with chloramphenicol. The isogenic strains were designated 35000hep, 35000glu, and 35000hepglu.

Characterization of the isogenic mutants. Outer membrane protein profiles and in vitro growth characteristics for all mutants did not reveal any significant differences in comparison to the wild type (data not shown). The LOS from the isogenic mutants, along with the wild-type strain 35000 and HD35000R, were analyzed by SDS-PAGE and Western blotting. Figure 3A shows that 35000hep synthesized multiple LOS glycoforms (lane 3), with a migration pattern similar to that of the wild-type LOS (lane 1). The LOS of 35000glu (lane 5) migrated more rapidly than the wild-type LOS (lane 1) but slower than HD35000R (lane 2). This observation suggests that 35000glu LOS contains the complete triheptose core of the wild-type LOS. 35000hepglu synthesized a highly truncated LOS molecule which migrated identically to HD35000R (lane 7). The corresponding Western blot probed with MAb 3F11 (Fig. 3B) revealed prominent reactivity to a LOS band which is consistent with the principal LOS glycoform synthesized by strain 35000 (lane 1). In addition, the antibody also reacted to a larger glycoform which appeared to represent a minor component of the wild-type LOS. In comparison, MAb 3F11 reacted with similar intensity to two prominent bands in the heptosyltransferase mutant (lane 3). Closer inspection reveals a slight downward shift in the migration pattern of these two glycoforms (lane 3) compared to the bands detected in the wild-type LOS (lane 1). This minor shift was thought to be the result of the absence of the third heptose of the triheptose core in both LOS species detected. Subsequent MALDI-MS analysis confirmed this hypothesis and provided detailed structures for each LOS species synthesized by all the mutants (see below). MAb 3F11 did not react to LOS from 35000glu and 35000hepglu (Fig. 3B, lanes 5 and 7).

View larger version (118K): [in this window] [in a new window]   FIG. 3.   Composite of a silver-stained SDS-polyacrylamide gel (A) and Western blot (B) probed with MAb 3F11. Shown are LOS from 35000 (lane 1), HD35000R (lane 2), 35000hep (lane 3), 35000hep(pHEP) (lane 4), 35000glu (lane 5), 35000glu(pGLU) (lane 6), 35000hepglu (lane 7), and 35000hepglu(pHEPGLU) (lane 8). The LOS glycoforms that react with the MAb are marked with arrows.

Structural analysis. Mass spectrometric analysis of the O-deacylated LOS prepared from the three isogenic mutants confirmed the expected structures (Fig. 4B to D) and were clearly shifted in mass compared to the parental strain 35000 (Fig. 4A). The O-deacylated LOS obtained from wild-type strain 35000 gave a series of peaks, the four most abundant corresponding to (M  H) ions for LOS glycoforms terminating in N-acetyllactosamine (m/z 2,710.5), previously referred to as A₅ (7), and sialyl-N-acetyllactostamine (m/z 3,001.9, A₅ + 291 Da), both of which were additionally substituted with a single PEA group (m/z 2,833.9 and 3,124.8, respectively). An additional molecular ion at m/z 2,548.2 appears to arise from a loss of galactose from the terminal N-acetyllactosamine unit (A₅  162 Da). These masses and their assignments were previously reported for the human-passaged wild-type strain (7).

View larger version (26K): [in this window] [in a new window]   FIG. 4.   Negative-ion MALDI-MS spectra of O-deacylated LOS from wild-type strain 35000 (A) 35000glu (B), 35000hep (C), and 35000hepglu (and HD35000R) (D). MS spectra for HD35000R and 35000hepglu were essentially identical.

Complementation of isogenic mutants. To confirm that the LOS phenotypes observed for the isogenic mutants were not the result of secondary mutations, each of the mutants was complemented with the corresponding wild-type genes. Plasmids containing the individual genes (pHEP and pGLU) or both genes (pHEPGLU) were electroporated into each of the respective H. ducreyi mutants and immunoscreened using MAb 3F11. As a control, the pLS88 vector was used to electroporate each of the mutants. The presence of vector alone in the mutants had no effect on reactivity to MAb 3F11 or migration of LOS by SDS-PAGE (data not shown). All kanamycin-resistant transformants tested from the 35000glu and 35000hepglu complementations reacquired reactivity with MAb 3F11, indicating that expression of the principal LOS glycoform had been regained at the bacterial surface (data not shown). As shown in Fig. 3A, there was a major LOS glycoform, from the complemented mutant strains 35000glu(pGLU) (lane 6) and 35000hepglu(pHEPGLU) (lane 8), with an apparent mass equivalent to the principal LOS glycoform expressed by the wild type (lane 1). In addition, there is a larger glycoform observed in the LOS of 35000hepglu(pHEPGLU) (lane 8). The Western blot, probed with MAb 3F11 (Fig. 3B), demonstrated reactivity to a 4.5-kDa band in the complemented mutant LOS (lanes 6 and 8) and parental LOS (lane 1), as predicted. There is also reactivity to a larger glycoform detected in both the wild-type LOS (lane 1) and the LOS of 35000hepglu(pHEPGLU) (lane 8). This reactivity was consistent with the reactivity observed in the original complementation of HD35000R (lane 3), suggesting that this larger LOS glycoform contains di-N-acetyllactosamine.

Structural analysis of the LOS from complemented mutants. MALDI-MS analysis of the O-deacylated LOS prepared from three complemented strains revealed partial to complete complementation of the corresponding glycosyltransferase genes (Fig. 5B to D). The MALDI-MS spectrum of the O-deacylated LOS from the complemented isogenic mutant 35000glu(pGLU) (Fig. 5B) showed peaks at m/z 2,548.0, 2,710.2, 2,833.4, 3,001.5, and 3,124.4, identical to molecular ion masses observed in the parental LOS phenotype (Fig. 5A, wild-type strain 35000). The complemented isogenic mutant 35000hep(pHEP) also regained expression of several parental LOS glycoforms with (M  H) peaks at m/z 2,548.6, 2,710.5, and 2,833.6 (Fig. 5C). However, the sialylated glycoforms containing terminal NeuAc23Gal14GlcNAc were not observed.

View larger version (26K): [in this window] [in a new window]   FIG. 5.   Negative-ion MALDI-MS spectra of the molecular ion region of O-deacylated LOS from wild-type strain 35000 (A), complemented mutant strain 35000glu(pGLU) (B), complemented mutant strain 35000hep(pHEP) (C), and double-complemented mutant strain 35000hepglu(pHEPGLU) (D) containing masses indicative of complete complementation (bold) and incomplete (italics). See text for details.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

Pyocin lysis has previously been used as a strategy to identify LOS mutants of H. ducreyi and N. gonorrhoeae (8, 14). Although the actual mechanism of lysis is poorly understood, a recent report by Lee et al. suggests that these particles contain nucleic acid (27). In this study, we used pyocin to select for LOS mutants of H. ducreyi strain 35000. One such mutant, HD35000R, produced a LOS molecule that lacked the MAb 3F11 epitope and migrated with an increased mobility on SDS-PAGE. Complementation of this mutant with a plasmid library containing H. ducreyi 35000 chromosomal DNA resulted in the identification of a clone expressing wild-type LOS. Western blot analysis confirmed that this transformant, HD35000R(pLS88.8), expressed the LOS epitope reactive with MAb 3F11. The sequence analysis of the complementing plasmid revealed a 3.2-kb DNA insert containing four complete ORFs. The putative protein product of ORF1 shared similarity (37%) with the E. coli WaaQ, the heptosyltransferase responsible for addition of the third heptose residue to the inner core of E. coli lipopolysaccharide (LPS) (44). The H. ducreyi WaaQ homologue also exhibited similarity to the WaaF homologue of H. ducreyi, which may function to attach the HepII via an 1,3 linkage to the HepI of the LOS core (5). However, MALDI-MS analysis confirmed that the core of HD35000R lacked the third heptose; therefore, we concluded that the waaQ encodes the H. ducreyi heptosyltransferase III.

The predicted amino acid sequence of ORF2 was similar (49%) to the sequence of LgtF of N. meningitidis, a 1,4-glucosyltransferase (24). This is the first carbohydrate added to the heptose core on the main oligosaccharide branch of the principal glycoform synthesized by wild-type H. ducreyi 35000. Again, based on this sequence homology and subsequent structural analysis of the LOS of HD35000R, we designated ORF2 as the lgtF homologue in H. ducreyi. To confirm that the cloned genes were responsible for the proposed functions, isogenic mutants were constructed and then complemented with the corresponding wild-type genes.

Inactivation of the H. ducreyi lgtF gene resulted in the expression of a truncated LOS molecule lacking the first glucose of the oligosaccharide branch and all subsequent sugars distal to this carbohydrate. However, this disruption did not affect the assembly of the triheptose inner core. This result confirms that the H. ducreyi lgtF is the homologue of the 1,4 glucosyltransferase of N. meningitidis (24). Furthermore, our data confirm that the expression of this protein is required for chain elongation extending from the HepI of the core of H. ducreyi LOS.

Recently it was reported that a mutation in E. coli waaQ (heptosyltransferase III) resulted in synthesis of a LPS molecule which migrated similarly to wild-type LPS, lacking only the third heptose of the inner core (44). Inactivation of H. ducreyi waaQ also resulted in the expression of LOS molecules with electrophoretic mobility patterns that were similar, but not identical, to those of the LOS glycoforms produced by the wild-type strain. Structural analysis confirmed that these LOS molecules lacked only the third heptose of the core. The full-length LOS structure produced by the waaQ mutant demonstrates that the glucosyltransferase (LgtF) functions in the absence of a complete triheptose core. In addition, the reactivity to MAb 3F11 provided evidence that the LOS glycoforms of the waaQ mutant, lacking the third heptose of the inner core, retained the proper conformation of the terminal lactosamine epitope recognized by this antibody.

Interestingly, the H. ducreyi waaQ mutant also synthesized additional LOS glycoforms, albeit in minor abundance, that were not detected in the LOS of the wild-type strain. MALDI-MS analysis determined that these larger glycoforms were primarily the result of the addition of di-, tri-, and polylactosamines to the terminal portion of the main LOS branch. Previously Melaugh et al. detected that the dilactosamine glycoform existed in LOS isolated from H. ducreyi 35000, and these investigators speculated that this structure may have arisen from an alternative, as yet undefined, biosynthetic pathway (29). Complementation of 35000hep mutant resulted in production of wild-type LOS and the disappearance of the slower-migrating LOS glycoforms. This result suggests that the synthesis and addition of terminal lactosamine may be directly or indirectly linked to the amount of WaaQ expressed during the synthesis of H. ducreyi LOS. This hypothesis is further supported by the fact that complementation of the 35000hepglu mutant, with the plasmid containing only the lgtF gene, resulted in appearance of LOS structures consistent with the multiple glycoforms detected in the 35000hep strain (data not shown). While it is intriguing to speculate as to possible regulatory mechanisms involved in the biosynthetic pathway of H. ducreyi LOS, more detailed studies are clearly needed before any conclusions can be made.

The mutant disrupted in both waaQ and lgtF (35000hepglu) produced a LOS molecule that migrated with a mobility identical to the mobility of the principal glycoform produced by HD35000R. This result suggests that HD35000R contains disruptions in both LOS biosynthesis genes, and we are currently attempting to amplify the waaQ and lgtF genes from HD35000R to determine if both of these genes are disrupted by insertions, deletions, or point mutations. This information could provide more insight into possible regulation of these LOS genes. We also considered the possibility that HD35000R has a mutation in a regulatory gene that controls expression of both transferase genes. However, this scenario is highly unlikely, because these genes are found to be adjacent to one another and are transcribed convergently. While genes involved in LPS biosynthesis have been shown to be in contiguous clusters, LOS genes for many gram-negative human pathogens have been shown to be scattered throughout the chromosome (35). In addition to the downstream sequence, the sequence 5' of the waaQ gene was determined to contain a homologue to the argininosuccinate synthase of H. influenzae (data not shown) (16). Therefore, our sequence analysis upstream and downstream of the waaQ and lgtF confirmed that these genes were not arranged in a contiguous cluster with other LOS synthesis genes, which is consistent with findings for other gram-negative bacteria such as N. meningitidis, N. gonorrhoeae, and H. influenzae (35).

It is also interesting that sialic acid is absent among LOS glycoforms terminating in N-acetyllactosamine in the 35000hep mutant and in some of the complemented strains (Fig. 6). Although this phenomenon is unexplained, there are multiple factors that could be involved in the synthesis and addition of sialic acid. The gene which encodes for the H. ducreyi sialyltransferase has been cloned and sequenced (7). This enzyme has been reported to be unique in comparison to other known sialyltransferases, and there are currently no data describing the regulation of sialic acid addition to H. ducreyi LOS. It is possible that factors such as growth conditions and media may effect this mechanism. The presence of a complete LOS core or the presence of the proper accepting terminal region of the LOS molecule could also be critical. Most likely a combination of multiple factors is involved in this complex process. Although the mechanism of sialylation of H. ducreyi LOS is beyond the scope of this study, we now have the constructs that are essential to further investigations designed to understand the sialylation of H. ducreyi LOS.

View larger version (20K): [in this window] [in a new window]   FIG. 6.   Structure of LOS from isogenic strains 35000 (wild type [WT]) (A), 35000hepglu (identical to strain 35000R) (B), 35000glu (C), and 35000hep (D). Expected masses for deprotonated molecular ions, (M  H), for O-deacylated LOS are indicated. All core heptoses are L-glycero-D-manno-heptose with the exception of the branch heptose (labeled with an asterisk) which is D-glycero-manno-heptose.

In conclusion, we have identified, cloned, and sequenced two genes involved in expression and biosynthesis of the principal LOS glycoform of H. ducreyi 35000. Isogenic mutants deficient in expression of the heptosyltransferase III, the 1,4-glucosyltransferase and both glycosyltransferase genes were constructed and characterized. Future studies will be aimed at comparing these LOS mutants with the parental strain in biological assays such as adherence and invasion of human keratinocytes. In addition, these mutants can be compared with the wild type in the human challenge model of infection. Such studies may increase our knowledge of the mechanisms that control the regulation of expression of these glycosyltransferases and the role of LOS in pathogenesis of this organism.