TEXT

Top Abstract Text References

Streptococcus pneumoniae is the major cause of otitis media, pneumonia, and meningitis. Recent studies have presented new insights into the pathogenesis of pneumococcal infection (17). In addition to the antiphagocytic polysaccharide capsule, several proteins have been described as virulence factors for S. pneumoniae, most of which are located on the pneumococcal cell surface and contribute to colonization, adherence, and invasion during infection of various animal models (12, 13). We constructed a genomic expression library of an S. pneumoniae strain (strain 3.B, serotype 1; collection of the Department of Medical Microbiology and Virology, University of Duesseldorf) which was isolated from a patient suffering from disseminated pneumococcal infection. Here we describe the screening of this library with a serum which was taken from the patient during the convalescent phase, 26 days after diagnosis.

A number of pneumococcal proteins were highly reactive with immunoglobulin G (IgG) antibodies of the patient serum. Immunoblot analysis of pneumococcal cell lysates showed immunodominant bands from 60 up to 130 kDa (data not shown). To identify these immunoreactive proteins, an expression library of the pneumococcal genome was created in Escherichia coli. Pneumococcal DNA was digested partially by Sau3A, and fragments of 500 to 1,600 bp were ligated with the expression vector pET15b (Novagen) and used for transformation of E. coli. About 61,000 recombinants were screened for expression of proteins which were reactive with IgG antibodies of the convalescent-phase serum by colony immunoblot analysis. Seventy-eight recombinant proteins were detected. Western blot analysis of whole-cell lysates confirmed expression of immunoreactive proteins in 56 clones, from which plasmid DNA was isolated and the pneumococcal DNA was sequenced. The DNA sequences were used for a similarity search. The nucleotide sequence of the identified gene was completed by the sequence data obtained from the unfinished pneumococcal genome (available on the website of The Institute for Genomic Research [TIGR] [http://www.tigr.org]).

Identification of known pneumococcal proteins. In total, 20 clones contained pneumococcal DNA of previously identified genes. They contained eight different DNA inserts from six genes (Fig. 1). Five of the deduced proteins were surface located and one (DnaK) was presumably of cytoplasmic origin. Parts of the pneumococcal surface protein A (PspA) were expressed in three recombinants. PspA, a choline-binding protein, has been characterized as a lactoferrin-binding protein and was shown to be protective in immunization studies (6, 10). The protein is composed of a variable N-terminal domain followed by an 82-amino-acid proline-rich region and a C-terminal choline-binding domain composed of 10 highly conserved 20-amino-acid repeats (18). All three clones contained DNA coding for the proline-rich domain of PspA. The clone SP-52 expressed the N-terminal part of the protein, including the first 58 amino acids of the proline-rich domain. SP-11 and SP-30 encoded the C-terminal third of the proline-rich domain and parts of the flanking choline-binding domain. IgG antibodies of the convalescent-phase serum showed high reactivity with the overexpressed protein of clone SP-52 and showed low binding with the recombinant proteins SP-11 and SP-30. This is in agreement with previous results suggesting that the proline-rich domain has an important function in immunogenicity (3). Three recombinants contained DNA coding for the C-terminal part of the proline-rich and repetitive domain of the S. pneumoniae surface protein A (SpsA), another choline-binding protein. It has been shown that SpsA binds the secretory component of IgA and plays a role in pneumococcal adhesion to eukaryotic cells (7, 15). Another seven recombinants contained the 5' part of the neuraminidase A (nanA) gene. NanA has previously been identified as a surface protein on the basis of its C-terminal LPXTG motif. It has been assumed that the enzyme cleaves the terminal sialic acids on cell surface glycolipids, thereby exposing the carbohydrate ligand by which S. pneumoniae attaches to host cells (for review see reference 11). The recombinant SP-14 expressed the central part of -N-acetylhexosaminidase (StrH), an enzyme predicted to be anchored in the bacterial membrane by its C-terminal LPXTG motif. The protein is one of six pneumococcal glycosidases. It has been assumed that StrH plays a role in pathogenesis since GlcNAc1-linked residues are common components of several cell surface molecules on host tissue cells (4). Five clones expressed parts of the AliB protein, a membrane-bound lipoprotein and part of a multicomponent oligopeptide transporter. The protein has been suggested to play a role in signal transduction and genetic competence (1, 2). The pneumococcal DNA cloned into SP-50 coded for the central region of AliB, and the four recombinants SP-18, -59, -67, and -68 expressed the N-terminal region of the protein. The N-terminal region of the heat shock protein DnaK (hsp70 family) was identified in SP-39. DnaK is one of three pneumococcal heat shock proteins. Immunoblot analysis with mouse antipneumococcal sera has revealed that DnaK is a major immunogen (5, 9). According to the PSORT analysis of the deduced amino acid sequence, DnaK appears to be a cytoplasmic protein, in contrast to the other proteins described above.

View larger version (21K): [in this window] [in a new window]   FIG. 1.   Immunogenic pneumococcal proteins of known function. The parts of the proteins which were encoded by the cloned DNA inserts of the expression library are hatched. The intensity of IgG binding to the expressed proteins on the Western blot using whole-cell lysates of E. coli recombinants is indicated. x, no reactivity; +, ++, and +++, weak, medium, and strong reactivities, respectively; N, native conditions; D, denaturing conditions.

Identification of unknown pneumococcal proteins. Twenty distinct inserts corresponding to 17 genes not previously described were identified in 36 recombinant plasmids, all of which expressed IgG-reactive proteins. The entire nucleotide sequence and the corresponding amino acid sequence for 15 of the 17 detected unknown pneumococcal genes could be deduced by comparison with the preliminary sequence data of the pneumococcal genome (TIGR website). PSORT analysis of the proteins was used for the prediction of their cellular localization. In addition, these amino acid sequences were subjected to a similarity search through the National Center for Biotechnology Information database, using the BLAST programs (Table 1). Four of these proteins showed similarity with enzymes involved in carbohydrate metabolism, all of which appeared to be membrane bound. Three proteins, which were homologs of -galactosidase, endo--N-acetylglucosaminidase, and amylopullulanase, showed strong IgG binding using the convalescent-phase serum. Four deduced proteins were also homologs of metabolic enzymes and probably located in the cytoplasm. Another three were homologs to ATP-binding cassette (ABC) transporters, whereby the two membrane-localized proteins were more immunogenic than the cytoplasmic SP-69 homolog. Among the remaining proteins, a homolog to a cell wall-associated serine proteinase was detected. Also, a putative RNA helicase (a member of the DEAD protein family) revealed strong binding to IgG under nondenaturing conditions. Remarkably, this protein showed 61% amino acid similarity to the autoaggregation protein AggH of Lactobacillus reuteri (14). In addition, one deduced protein was highly related to DtxR, an iron-dependent regulatory DNA binding protein of Corynebacterium diphtheriae (16). Finally, four proteins were identified in this screen which showed no homology to other known bacterial proteins.