INTRODUCTION

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Nitric oxide (NO) plays important roles in many different aspects of mammalian biology (25). In phagocytes, NO is produced in large quantities by the action of the enzyme inducible NO synthase (iNOS), also known as type II NOS (20, 33). This isoform is produced following stimulation of cells with agents such as inflammatory cytokines and lipopolysaccharide (LPS). Studies using NOS inhibitors and mice with targeted disruption of the iNOS gene have demonstrated an important role of NO release from this enzyme in host defense against a number of intracellular pathogens, including Mycobacterium tuberculosis, Leishmania major, Listeria monocytogenes, and Salmonella enterica serovar Typhimurium (26, 32).

NO is a highly reactive free radical with antimicrobial activity on its own, but it also can form a number of oxidation products such as NO₂, NO₂, N₂O₃, and S-nitrosothiols. In addition, it reacts with superoxide to yield the extremely reactive and microbicidal peroxynitrite anion, ONOO (2, 41). Collectively, these reactive nitrogen intermediates have a spectrum of antimicrobial activity mediated by their ability to react with key protein and lipid molecules in microbes (18, 29). These cytotoxic effects are not restricted to microbes, as reactive nitrogen intermediates can act on host cells to produce cell necrosis or apoptosis, thus potentially exacerbating tissue injury where produced (5, 19).

This balance between microbicidal activity and potential cytotoxicity applies to many of the antimicrobial products produced by phagocytes, such as lysosomal enzyme products and superoxide. How do these cells avoid the damaging effects of these chemicals, while still efficiently killing invading pathogens? One mechanism is by compartmentalizing these toxic compounds within the cell so that they are released in close proximity to the pathogen, for example, by fusion of lysosomes with phagosomes or targeting of the superoxide-generating cytochrome b to neutrophil-specific granules (4, 8, 23). Pathogens also have strategies to avoid these compartmentalized killing compounds. For example, S. enterica serovar Typhimurium disrupts intracellular trafficking and avoids fusion with lysosomal contents (7). It also prevents movement of active NADPH oxidase to the Salmonella vacuole (38). This effect on the movement of NADPH oxidase is mediated by a type III secretion system encoded within Salmonella pathogenicity island 2 (SPI-2) (38). However, NO still has an important antimicrobial effect against serovar Typhimurium, mainly by exerting a relatively delayed bacteriostatic effect within macrophages (37).

In neutrophils, we have shown that iNOS is localized to primary granules, where it is able to mediate nitration of ingested bacteria, most likely through the generation of peroxynitrite (13). In macrophages, although the enzyme is frequently described as cytoplasmic, some reports have shown that a proportion of the enzyme is localized to a particulate fraction within the cell (17, 30). One study has addressed the subcellular localization of iNOS within primary macrophages, using immunoelectron microscopy (39). This found that a proportion of iNOS was present in a population of vesicles, most notably in the trans-Golgi network. The authors reported that iNOS was not associated with phagosomes containing Mycobacterium avium or Leishmania mexicana, although a preliminary association of iNOS vesicles with phagosomes containing immunoglobulin G (IgG)-coated beads was mentioned. However, no images of the distribution of iNOS following phagocytosis were presented.

Once iNOS has been induced within the macrophage, there appear to be few further controls over its activity (27), although the availability of tetrahydrobiopterin and L-arginine may be important (31, 34). Given the high reactivity of NO, we postulated that one way in which the cell could control the delivery of NO to its targets would be by subcellular compartmentalization, specifically to phagosomes. Using high-resolution laser confocal immunofluorescence microscopy and biochemical techniques, we found in both primary murine macrophages and the macrophage cell line RAW264 stimulated with LPS and gamma interferon (IFN-) that a proportion of iNOS was associated with the cortical submembranous actin cytoskeleton, as well as in intracytoplasmic vesicles and in free cytoplasm. Following phagocytosis of a variety of particles or the pathogen S. enterica serovar Typhimurium, membrane-associated iNOS did not relocalize around phagosomes, nor was there recruitment of cytoplasmic iNOS to phagosomes. However, NO mediated a significant killing effect against serovar Typhimurium within 4 h of infection in LPS- and IFN--treated cells that may be mediated by membrane-associated iNOS.

	MATERIALS AND METHODS

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Reagents and antibodies. Rabbit polyclonal (N32030) and murine monoclonal (N39120) antibodies to iNOS were from Transduction Laboratories, Lexington, Ky., as was mouse monoclonal antibody to GM130. Goat polyclonal anti-LAMP1 was from Santa Cruz Biotechnology. Secondary antibodies conjugated to Alexafluors were from Molecular Probes (Eugene, Oreg.). Biotinylated secondary antibodies to mouse, rabbit, or goat immunoglobulin were from Jackson ImmunoResearch Laboratories, West Grove, Pa., or Vector Laboratories, Peterborough, United Kingdom. Fluorescein isothiocyanate or Texas red avidin was bought from Vector Laboratories. Latex beads and zymosan particles were from Sigma-Aldrich, Poole, United Kingdom. Wild-type Salmonella enterica serovar Typhimurium 12023, its corresponding SPI-2 mutant, and the green fluorescence protein-labeled wild-type strain were as previously described (3).

Cell culture. To obtain peritoneal macrophages, CD1 mice were primed with thioglycolate broth or BioGel P100 beads (Bio-Rad, Hercules, Calif.). Three days later, peritoneal fluid was collected and cells were washed in Hanks balanced salt solution (Life Technologies Ltd., Paisley, United Kingdom) and plated out for 2 h at 37°C. Adherent macrophages were then washed free of other cells. Cells were seeded onto glass coverslips (Merck Ltd., Poole, United Kingdom) in 24-well dishes (Corning Costar, High Wycombe, United Kingdom) at 10⁶ cells ml¹. Both RAW 264 cells and primary macrophages were cultured in Dulbecco's modified Eagle's medium (Life Technologies Ltd.) with 10% heat-inactivated fetal calf serum (Labtech International, Ringmer, United Kingdom) and 1 mM glutamine (Life Technologies Ltd.). Where indicated, cells were stimulated for 18 h with 10 ng of IFN- (Peprotech EC Ltd., London, United Kingdom) ml¹ and 2 µg of LPS from Escherichia coli serotype 0111:B4 (Sigma-Aldrich) ml¹. If used, cytochalasin B (Sigma-Aldrich) was added to cells at 10 µM for 30 min. Particles were centrifuged onto the macrophages at 450 × g for 2 min, normally at a ratio of 10 to 1. Zymosan was prepared by being boiled in phosphate-buffered saline (PBS; 0.01 M sodium phosphate buffer [pH 7.3], 0.15 M NaCl) for 30 min. Bacteria were used at stationary phase or logarithmic phase, opsonized in 10% mouse serum (Serotec Ltd., Oxford, United Kingdom) for 15 min at 37°C, and then washed three times with PBS before being added to macrophages.

Immunostaining. iNOS was detected by immunocytochemistry using either a murine monoclonal iNOS antibody or a rabbit polyclonal anti-iNOS (both from Transduction Laboratories). The monoclonal antibody recognizes a single 135-kDa protein, the expected weight of iNOS, in cytokine-treated human and murine cells (28, 40). After incubation, cells were washed gently three times with PBS and left to dry. Cells were fixed in 1% paraformaldehyde for 30 min, washed in PBS, and then blocked in 0.2 M glycine in PBS for 15 min. Cells were washed three times in PBS and then permeabilized in 0.1% Triton X-100 in PBS for 15 min. After further washes in PBS, cells were blocked in 10% serum (Serotec Ltd.), using animal serum corresponding to the host in which the secondary antibody was derived. After at least 30 min of incubation, the block was removed and primary antibody to iNOS was added. Cells were incubated for 18 h at 4°C. After three 5-min washes in PBS, secondary antibody to mouse or rabbit was added either using Alexafluor conjugates (Molecular Probes Europe BV, Leiden, The Netherlands) or adding the appropriate biotinylated secondary antibody (Vector Laboratories or Jackson ImmunoResearch Laboratories) and then adding fluorescein isothiocyanate or Texas red-conjugated avidin (Vector Laboratories). Tetramethyl rhodamine isocyanate-phalloidin (Sigma-Aldrich) was used to detect filamentous actin. After washing, DAPI (4',6'-diamidino-2-phenylindole) (Sigma-Aldrich) was added to stain nuclei. Cells were mounted in Vectashield (Vector Laboratories). Immunofluorescent images were acquired by confocal microscopy, using a Zeiss Axiovert microscope and the Bio-Rad MRC 1024 Confocal system, running with LaserSharp software.

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. To extract sufficient protein for Western analysis, 12 175-cm² flasks of RAW cells were used for each fractionation, using a published method (35). Briefly, cells were stimulated with 10 ng (200 U) of IFN- per ml and 2 µg of LPS ml¹ for 18 h and then scraped into 1 ml of lysis buffer (0.25 M sucrose, 3 mM imidazole, 0.5 mM EDTA [pH 7.3]) containing 1 µg each of leupeptin, aprotonin, chymotrypsin inhibitor, and antipain ml¹. Cells were centrifuged at 750 × g for 7 min, and the cell pellet was resuspended in 100 µl of lysis buffer. This suspension was passed through a 21-gauge needle until cell breakage occurred, and then it was centrifuged at 750 × g for 7 min to remove nuclei. The supernatant was then centrifuged at 100,000 × g for 2 h to separate soluble and particulate fractions. The pellet, containing particulate material, was resuspended in 1 ml of lysis buffer, vortexed gently, and left on ice for 30 min. It was then layered onto 9 ml of 17% Percoll, which was above a cushion of 2 ml of 64% sucrose, both made up in lysis buffer. Tubes were centrifuged at 56,000 × g for 1 h (without the brake) and 1-ml fractions were recovered by puncturing the tube. Fractions were run on an 8% acrylamide sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel and blotted onto a polyvinylidene difluoride membrane (Amersham Pharmacia Biotech UK, Little Chalfont, United Kingdom). iNOS was detected with a monoclonal iNOS antibody (Transduction Laboratories) and biotinylated secondary anti-mouse antibody (Vector Laboratories and Jackson ImmunoResearch Laboratories), horseradish peroxidase-streptavidin reagent (Serotec Ltd.), and ECL plus detection (Amersham Pharmacia Biotech UK). To identify cell surface proteins, cells were treated with biotinylation reagent EZ-Link Sulfo-NHS-LC-Biotin (Pierce Chemical Company, Rockford, Ill.) before being scraped into lysis buffer. To identify fractions containing transferrin (early endosomes), 20 nM biotin-transferrin (Molecular Probes) was added to unstimulated RAW cells and incubated for 20 min at 37°C before being washed with PBS, and cells were scraped from the flask in 1 ml of lysis buffer.

iNOS activity assay. Fractions and totals were assayed for iNOS activity by following the conversion of [³H]arginine to citrulline (NOSdetect Assay Kit; Stratagene, Amsterdam, The Netherlands). To duplicate reaction mixtures, we added 2 mM L-NIL hydrochloride (Calbiochem-Novabiochem [UK] Ltd., Nottingham, United Kingdom) to identify iNOS-dependent conversion to L-citrulline. Reactions were carried out according to the kit instructions, and L-citrulline was recovered by adding the reaction mixture to columns containing DOWEX 50WX8-400 ion-exchange resin (Sigma-Aldrich) and washing it three times with water to elute citrulline. The quantity of isotope was measured by liquid scintillation counting.

-Glucuronidase assay. To identify fractions containing lysosomes, we assayed the presence of -glucuronidase activity by using a diagnostic kit (Sigma-Aldrich). Briefly, 20 µl of each fraction was mixed with 20 µl of phenolphthalein glucuronic acid solution and 60 µl of acetate buffer solution and incubated at 56°C for 1 h. Five hundred microliters of 2-amino-2-methyl-1-propanol buffer was added, and absorbance was read at 550 nm.

Assay of intracellular Salmonella viability. Monolayers of RAW macrophages in 24-well plates (10⁶ cells per well) treated as described above were infected with opsonized Salmonella enterica serovar Typhimurium 12023 in the stationary phase of growth at a multiplicity of infection of 10. To ensure a synchronous infection, after the addition of bacteria plates were centrifuged at 200 × g for 4 min and then incubated at 37°C for 30 min. Gentamicin (12 µg ml¹) was then added to kill extracellular bacteria, and incubation was continued as required. Viable intracellular bacteria were assayed by washing the well twice with PBS, lysing the cells in 100 µl of 1% Triton X-100 for 10 min at room temperature, and plating serial dilutions onto prewarmed LB agar plates. After overnight culture at 37°C, CFU were enumerated. Each experimental point was determined in triplicate.

Statistical analysis. Results are given as the means plus or minus standard deviations. To calculate the percentage of cells expressing iNOS in the plasma membrane, a total of >50 iNOS-positive cells were scored in three to four separate experiments.

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

Immunofluorescent microscopic localization of iNOS within mouse macrophages. We used a mouse monoclonal anti-iNOS and a polyclonal rabbit anti-iNOS. The pattern of staining was identical with both antibodies used; isotype-matched control sera gave no immunostaining using both anti-mouse and anti-rabbit secondary reagents. Unstimulated cells showed only 0 to 1% iNOS-positive cells.

View larger version (82K): [in this window] [in a new window]   FIG. 1.   Localization of iNOS within macrophages. Primary mouse peritoneal macrophages (A to C) or RAW 264 cells (D) were stimulated with IFN- alone (A) or with IFN- and LPS (B to D) and immunostained for iNOS, using the mouse monoclonal antibody. All images were obtained using laser confocal microscopy. Magnification, ×2,000 (A), ×600 (B to C), or ×6,000 (D). Arrows indicate peripheral plasma membrane staining of iNOS. The solid arrowhead in panel B indicates iNOS staining in the perinuclear area.

View larger version (10K): [in this window] [in a new window]   FIG. 2.   iNOS can be found in the Golgi apparatus. RAW 264 cells were stimulated with LPS and IFN- and dually stained for iNOS using a rabbit polyclonal antibody, with red staining (A), and the intrinsic Golgi protein GM130, with green staining (B). (C) Merged images of panels A and B, with areas of colocalization in yellow. Magnification, ×1,000 for all panels.

iNOS associates with actin at the cell periphery. We examined whether there was an association between iNOS and the cortical actin cytoskeleton that might account for its localization at the cell periphery. Using fluorescent phalloidin to visualize polymerized actin, we found that iNOS and polymerized actin colocalize at the cell periphery (Fig. 3A to C). When the cortical cytoskeleton was disrupted with cytochalasin B, cortical iNOS was completely disrupted, with iNOS distributed entirely within the cytoplasm (Fig. 3D to F). It is important to stress that following cytochalasin treatment, we were unable to detect any cell that showed cortical iNOS staining. These data suggest strongly that the peripheral iNOS is associated with the cortical actin cytoskeleton. To determine if any iNOS was exposed on the surface of cells, we immunostained macrophages for iNOS with no prior permeabilization step. Under these conditions, we observed no iNOS staining (data not shown). Taken together, these data suggest that iNOS associates with the cortical actin cytoskeleton at the cytoplasmic face of the plasma membrane.

View larger version (25K): [in this window] [in a new window]   FIG. 3.   iNOS associates with the cortical actin cytoskeleton. Primary peritoneal macrophages were stimulated with LPS and IFN- and immunostained for iNOS with the mouse monoclonal antibody in green (B and E) or polymerized actin in red (A and D). Cells were either untreated before staining (A to C) or treated with cytochalasin B (D to F). Panels C and F show the merged actin and iNOS images from panels A and B and panels D and E, respectively; areas of colocalization are shown in yellow. Magnification, ×600 for all panels.

Biochemical fractionation of iNOS within RAW 264 cells. In order to explore the subcellular distribution of iNOS further, we fractionated RAW 264 cells using biochemical techniques. We chose these cells for further study, as it was difficult to obtain enough material from primary peritoneal macrophages without sacrificing large numbers of animals. Particulate and soluble fractions were analyzed for the presence of iNOS by Western blotting. We found that 32.75% (±5.11%; n = 3) of total cellular iNOS was recovered in the particulate fraction as assayed by densitometry of immunoblots, in good agreement with other studies (30, 39). The activity of this particulate material was also measured and found to be 16.47% (±0.15%; n = 3) of total cellular iNOS activity. The smaller amount of iNOS activity in the particulate material, compared to the amount of iNOS protein, may reflect poor access of substrates and cofactors to the particulate enzyme or some denaturation on centrifugation, or it may truly reflect a difference in specific activity of iNOS in cytosolic and particulate locations.

View larger version (24K): [in this window] [in a new window]   FIG. 4.   Density gradient centrifugation analysis of the particulate fraction of IFN-- and LPS-treated RAW 264 cells. Each panel shows an assay performed on each of 12 fractions from the density gradient, with the lighter fractions to the left (top) and the denser ones to the right (bottom). The experiment was repeated with similar results. (A) iNOS protein, detected by Western blotting. The band shown is the major band detected, at 135 kDa. So that the bands in lanes 6 to 11 could be seen, the blot was overexposed for lanes 1 to 3. (B) iNOS activity, as assayed by arginine-to-citrulline conversion. (C) Surface proteins of the cell, labeled by surface biotinylation, identified by probing the Western blot with horseradish peroxidase-streptavidin. The gel shows proteins ranging in size from 200 kDa at the top of the panel to 29 kDa at the bottom. (D) -glucuronidase activity.

Patterns of iNOS localization following phagocytosis. Next, we examined the pattern of iNOS staining within macrophages following phagocytosis of a variety of particles. These included latex beads, zymosan, IgG-coated magnetic beads, and complement-coated zymosan particles. Representative results from experiments with primary macrophages are shown in Fig. 5. Panels A and B show iNOS staining (green) following phagocytosis of latex beads for a 30-min period. iNOS staining remained at the periphery of the cells and did not localize to phagosomes. Similar results were seen at much earlier time points (2 and 5 min of particle internalization; data not shown), with no localization of iNOS to phagosomes at any stage of bead uptake. Following internalization of latex beads, phagosomes were allowed to mature for a further 2 h and the pattern of iNOS distribution was analyzed (Fig. 5D to F). This showed that the majority of latex bead-containing phagosomes had no accumulation of iNOS at their periphery (Fig. 5D). There were occasional phagosomes that showed iNOS accumulation at their periphery (Fig. 5D). However, this was seen in only fewer than 1% of the cells. In contrast, after this period of maturation, most phagosomes showed accumulation of the lysosomal marker LAMP-1 (Fig. 5E and F) confirming the fusion of lysosomes with phagocytosed latex beads at this time after ingestion.

View larger version (63K): [in this window] [in a new window]   FIG. 5.   iNOS distribution following phagocytosis in mouse primary peritoneal macrophages. Cells treated with IFN- and LPS were used in all panels. (A and B) Uptake of latex beads at 30 min after addition. iNOS staining (mouse monoclonal) in green is shown alone (A) and also merged with the differential interference contrast (DIC) image of the same cell (B). (C) A cell, 2 min after addition of zymosan particles, stained for iNOS (mouse monoclonal) in green. Accumulation of iNOS at the tips of pseudopodia closing around zymosan just entering the cell is shown by arrows. (D to F) Cells, 2 h after being fed latex beads for 10 min, which were then removed by washing. The panels show the pattern of iNOS staining (mouse monoclonal) in green (D), LAMP-1 staining in red (E), and the merged images of panels D and E (F), where areas of colocalization are shown in yellow. Two areas showing apparent accumulation of iNOS around the outside of phagocytosed beads are indicated by arrows in panel D. (G and H) Uptake of IgG-coated magnetic beads at 5 min after addition. iNOS staining (rabbit polyclonal antibody) in green is shown alone (G) and merged with the DIC image of the same cell (H). (I) A cell, 10 min after the addition of complement-coated zymosan particles stained for iNOS (mouse monoclonal) in green. Magnification, ×2,000 for all panels.

iNOS localization following ingestion of S. enterica serovar Typhimurium. Using a virulent strain of S. enterica serovar Typhimurium labeled with green fluorescent protein, we analyzed the distribution of iNOS following phagocytosis of this microorganism (Fig. 6). In both primary cells (Fig. 6A) and RAW 264 macrophages (Fig. 6B), there was no localization of iNOS around the Salmonella vacuole at any stage of maturation (Fig. 6A and B and data not shown). Similar results were obtained using bacteria in either the stationary or logarithmic phase of growth. Serovar Typhimurium prevents the transport of NADPH oxidase to the Salmonella vacuole by a process that is dependent on a type III secretory system encoded by a pathogenicity island termed SPI-2 (32). We therefore examined the distribution of iNOS around phagosomes containing an SPI-2 mutant strain (Fig. 6C). At a time when NADPH oxidase had accumulated around phagocytosed bacteria of this strain (38), we did not observe any relocalization of iNOS (Fig. 6C). Furthermore, when we stained macrophages for nitrotyrosine, a marker of the formation of peroxynitrite anions, although this could be seen within cells, it did not localize around phagocytosed bacteria (Fig. 6D).

View larger version (29K): [in this window] [in a new window]   FIG. 6.   iNOS and nitrotyrosine in macrophages following phagocytosis of serovar Typhimurium. In all cases, the bacteria are labeled green. (A) iNOS (red; mouse monoclonal) in a primary macrophage 2 h after phagocytosis of wild-type stationary phase serovar Typhimurium. (B) iNOS (red; rabbit polyclonal) in a RAW 264 cell 90 min after phagocytosis of wild-type stationary phase serovar Typhimurium. (C) iNOS (red; rabbit polyclonal) in a RAW 264 cell 4 h after phagocytosis of a SPI-2 mutant stationary-phase serovar Typhimurium. (D) Nitrotyrosine staining (red) in a RAW 264 cell 4 h after phagocytosis of wild-type stationary-phase serovar Typhimurium. Magnification, ×1,000 (B and D) or ×2,000 (A and C).

NO effects on bacterial uptake by macrophages. The presence of iNOS at the plasma membrane is ideal for delivery of NO to potentially pathogenic microorganisms as they undergo phagocytosis. In a previous study of serovar Typhimurium survival within macrophages using IFN--primed macrophages, a delayed bacteriostatic effect of NO production on bacterial proliferation was observed (37). Therefore, a possible explanation of the lack of recruitment of iNOS to the Salmonella vacuole observed above is that we had not examined the cells at a point when NO was exerting its antibacterial effect. Therefore, we measured the time course of survival of Salmonella within RAW macrophages treated with (i) no additions, (ii) LPS and IFN-, (iii) LPS and IFN- and the NOS inhibitor L-NIL. Figure 7 shows that at 45 min after infection there was no significant difference in survival of intracellular Salmonella among the three groups of cells. However, at 4 h after infection there was a significant reduction in the numbers of intracellular Salmonella in the LPS- and IFN--treated cells compared to untreated cells, a difference that was abolished by the NOS inhibitor L-NIL (Fig. 7). Numbers of intracellular bacteria increased by 20 h after infection in all groups, although the difference between the LPS- and IFN--treated group and the others remained. Under these conditions, the NOS inhibitor L-NIL inhibited macrophage NO output by >95%, as detected by a Griess assay (data not shown). Thus, NO does exert an antibacterial effect in these cells, which is evident at 4 h after infectiona time when we observed no recruitment of iNOS to the Salmonella vacuole (Fig. 6C and data not shown).

View larger version (15K): [in this window] [in a new window]   FIG. 7.   Survival of serovar Typhimurium within RAW macrophages. Cells were infected at a multiplicity of infection of 10 with 107 bacteria at time zero. The graph shows the mean intracellular bacterial numbers found in RAW 264 cells either unstimulated (US), treated with LPS and IFN- (S), or treated with LPS and IFN- plus L-NIL (S+LNIL). Error bars, ± 1 standard deviation. A significant difference was found in intracellular Salmonella survival between cells treated with LPS and IFN- and cells treated with LPS, IFN-, and L-NIL at 4 and 20 h after infection. *, P < 0.05; ** P < 0.01 (two-tailed t test).

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

The work presented here reports the novel observation that a proportion of iNOS is associated with the cortical actin cytoskeleton in primary mouse macrophages and the murine macrophage cell line RAW 264. iNOS was also found in an intracellular vesicle population, within the Golgi apparatus, and diffusely within the cytoplasm. Following phagocytosis of a variety of particles, there was little evidence of recruitment of iNOS to phagosomes; neither was there relocation of iNOS around phagocytosed serovar Typhimurium. Although not recruited to the phagosome, cortical iNOS is ideally placed to deliver microbicidal NO to microorganisms in contact with the cell surface.

We also found iNOS diffusely within the cytoplasm, within cytoplasmic vesicles, and in a Golgi compartment (Fig. 1 and 2). The relative distribution of iNOS between these different subcellular sites is difficult to gauge from confocal microscopy, as pixel densities for each component would have to be calculated for multiple confocal sections through each cell to calculate the total distribution of iNOS. From the combined imaging, biochemical fractionation, and activity assays, we estimate that about 20% of iNOS is localized to the cortical region in macrophages following LPS and IFN- stimulation under the conditions used here.

What can we deduce about the functional role of the differentially distributed iNOS we have described? We originally hypothesized that iNOS would be targeted to phagosomes, in order to deliver a high concentration of NO to ingested microbes and limit possible damaging effects to the rest of the cell. Cortical iNOS is obviously well placed to deliver NO to incoming microorganisms that are being ingested. However, following phagocytosis of a variety of particles, cortical iNOS is not retained in or recruited to the phagosome (Fig. 5). Similarly, there was no association of iNOS with phagocytosed serovar Typhimurium (Fig. 6). These experiments utilized all the major molecular mechanisms for triggering phagocytosisthe Fc receptor, complement receptors, and the mannose receptor. It seems unlikely, therefore, that failure to observe recruitment of iNOS to the phagosome resulted from lack of signaling from cell surface receptors engaged during phagocytosis. Phagocytosis involves the internalization of a large proportion of the macrophage cell membrane, but only a very small proportion of plasma membrane proteins are removed as a result of this process, the majority being returned to their original locations (22). Integral membrane proteins such as Toll-like receptor 2 and natural resistance-associated macrophage protein 1 (N-RAMP1) are recruited to the phagosomal membrane (16, 36), although in the case of Toll-like receptor 2, this association can be transient. We analyzed iNOS localization around phagosomes at a range of times after formation, including very early stages at 2 min after particle internalization. We do not feel, therefore, that we missed a transient early recruitment of iNOS to the phagosome. The association of iNOS with the cortical actin cytoskeleton, on the cytoplasmic side of the cell membrane (Fig. 3), suggested that it might move with actin to the phagosomal membrane. However, not all actin-associated proteins show such a redistribution. For example, in phagocytosis of unopsonized zymosan, the actin-associated proteins vinculin and paxillin do not associate with phagosomes (1).

Despite the lack of recruitment of iNOS to phagosomes, NO clearly has important antimicrobial actions within macrophages, notably for serovar Typhimurium (37) (Fig. 7). In IFN--treated macrophages (37), this effect, although noticeable at 2 h after bacterial entry, was more marked 10 h after infection. We found that in LPS- and IFN--stimulated cells, the effect was somewhat earlier, with clear NO-dependent killing at 4 h after bacterial entry (Fig. 7). This difference may reflect the time taken for induction of iNOS in IFN--treated macrophages following bacterial ingestion. In LPS- and IFN--stimulated cells, high levels of iNOS are already present, so that the time course of Salmonella killing more accurately reflects the time of antimicrobial action of NO against the ingested microbe. However, in vivo, it may be more likely that macrophages are first activated with IFN- and then receive an LPS stimulus on phagocytosing Salmonella. Thus, the time course of Salmonella killing by macrophages in natural infection may be better reflected by cells activated with IFN- alone rather than LPS and IFN-.

iNOS association with the cortical actin cytoskeleton may be important in targeting NO to bacteria as they enter the cell, although the effects on phagocytosed serovar Typhimurium are not evident until 4 h after uptake of the bacteria into the cell. Alternatively, it may be that NO is capable of antimicrobial action without producing cytotoxicity, even if it is not delivered directly to the Salmonella vacuole. Unlike superoxide, NO can readily diffuse across membranes (10) and thus could enter the phagosome from the cytosol. This might account for the localization of the NADPH oxidase but not iNOS to the phagosomal membrane. In addition, the exact identity of the reactive nitrogen species responsible for anti-Salmonella activity is unknown. For example, NO may also produce potent antimicrobial compounds by reacting with cytosolic thiols to yield S-nitrosothiols. These reactions may be facilitated by cytosolic localization of NO production by iNOS. To determine the contribution of cortical actin-associated iNOS to Salmonella killing will require the construction of iNOS mutants that are specifically targeted to either plasma membrane or cytoplasm. Cells expressing these differently localized enzymes can then be assayed for their ability to affect intracellular Salmonella replication.

Serovar Typhimurium has evolved mechanisms which enable it to avoid contact with the NADPH oxidase within macrophages, as revealed by SPI-2 mutants (38). These mutants, however, also failed to show accumulation of iNOS around phagocytosed bacteria (Fig. 6C). Although Salmonella may have other genes which encode a factor able to prevent iNOS localization around phagosomes, we feel that this is very unlikely, since a variety of experimental particles also failed to show iNOS recruitment to the phagosome (Fig. 5). In addition, we did not observe any iNOS accumulation around phagocytosed E. coli, which enters but does not proliferate within macrophages (data not shown). It has been assumed that peroxynitrite, the product of NO and superoxide, is a major antimicrobial factor within macrophages (41). However, Vazquez Torres et al. (37) showed no localization of nitrotyrosine around intracellular Salmonella and found that the peroxynitrite scavenger uric acid potentiates rather than inhibits Salmonella killing. In addition, there was a clear temporal separation of the roles of NADPH oxidase and iNOS in Salmonella killing. Thus, it seems unlikely that peroxynitrite is responsible for macrophage killing of intracellular Salmonella. Although there was evidence of nitrotyrosine generation within macrophages in our experiments, we too found no localization around phagocytosed bacteria (Fig. 6D), unlike the extensive nitration around phagocytosed Staphylococcus aureus within neutrophils (13). It may be that bacterial peroxiredoxins in Salmonella are able to detoxify peroxynitrite within the vicinity of the bacterium, avoiding nitration (6). However, deletion of the gene for the small subunit of the peroxiredoxin alkylhydroperoxide reductase in serovar Typhimurium does not affect virulence, suggesting that such a mechanism is not critical for pathogenesis. An alternative explanation for the absence of nitration of intracellular Salmonella might be the abundance of periplasmic superoxide dismutase in the bacteria, limiting in situ formation of peroxynitrite from NO and superoxide (9, 14, 15).

One previous report analyzed the subcellular distribution of iNOS in primary macrophages using immunoelectron microscopy (39). These authors found that iNOS was both cytoplasmic and in a vesicle population, particularly at the trans face of the Golgi apparatus, and with some staining at the cell periphery. A notable feature of the cells showing the most marked membrane iNOS staining in the present study is that they had a rounded, nonactivated shape, even after LPS and IFN- stimulation. Thioglycolate-induced macrophages can show a more activated macrophage phenotype, with extension of multiple pseudopodia (12). Cells with this morphology tended not to show plasma membrane iNOS, suggesting that the signal involved in the generation of this phenotype leads to loss of iNOS from this site. Differences in macrophage activation state may thus account for differences in iNOS distribution.

Cortical iNOS might be involved not in microbial killing but in some other function. Recently, an essential role for NO derived from iNOS has been demonstrated in the signaling pathway of interleukin 12 (IL-12) and IFN-/ within NK cells (11). Plasma membrane-associated iNOS would be well placed to deliver NO locally to the IL-12 receptor signaling complex, maximizing its signaling effects while limiting toxicity within the cell. Macrophages have recently been shown to respond to combined IL-12 and IL-18 stimulation with an increased output of IFN- (24). Whether NO participates in the IL-12 signaling pathway in macrophages remains to be determined.

What mechanism underlies the association of iNOS with the cortical actin cytoskeleton? It has been found that in epithelial cells, iNOS is localized to the apical membrane of polarized cells (P. Glynne and T. J. Evans, submitted for publication). The interaction of iNOS with membranes from these epithelial cells shows that iNOS is a tightly bound peripheral protein, associating with the cortical actin cytoskeleton and requiring mixtures of salt and detergent for efficient solubilization. This suggests that similar molecular mechanisms are involved in tethering iNOS to the cortical region in all these cells. iNOS does not have an intrinsic membrane-spanning domain, nor does it have known lipid modifications, suggesting that the most likely mechanism is by a direct protein-protein interaction with a component of the cortical actin cytoskeleton. The Golgi association may thus reflect the trafficking of iNOS in association with this protein through the cell on its way to the cell cortex.

iNOS is an important part of the phagocyte's defensive capabilities, and the work described here sheds more light on the way in which it is controlled by the cell. Further work with additional pathogens will better define the precise role that membrane-associated iNOS has in microbial killing and may reveal additional functions of iNOS.