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Infection and Immunity, November 2001, p. 6970-6980, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.6970-6980.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

cDNA Array Analysis of cag Pathogenicity Island-Associated Helicobacter pylori Epithelial Cell Response Genes

Joanne M. Cox,1 Christopher L. Clayton,2 Toshihiko Tomita,1 Don M. Wallace,2 Philip A. Robinson,1 and Jean E. Crabtree1,*

Molecular Medicine Unit, St. James's University Hospital, Leeds LS9 7TF,1 and Genomics Unit, Glaxo Smith Kline Research and Development, Stevenage, Hertfordshire SG1 2NY,2 United Kingdom

Received 15 March 2001/Returned for modification 25 May 2001/Accepted 20 June 2001


    ABSTRACT
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

Helicobacter pylori strains containing the cag pathogenicity island (PAI) induce NF-kappa B activation and interleukin-8 secretion in gastric epithelial cells. The aim of this study was to investigate changes in epithelial gene expression induced by cag PAI-positive and -negative strains of H. pylori using high-density cDNA array hybridization technology. Radio-labeled cDNA prepared from H. pylori-infected Kato 3 gastric epithelial cells was hybridized to high-density cDNA arrays to identify changes in epithelial gene expression compared to noninfected controls. In vivo expression of selected, differentially expressed genes was examined by reverse transcription-PCR analysis of H. pylori-positive and -negative gastric mucosa. Screening of ca. 57,800 cDNAs identified 208 known genes and 48 novel genes and/or expressed sequence tags of unknown function to be differentially expressed in Kato 3 cells following H. pylori infection. Marked differences in gene expression profiles were observed following cag PAI-positive and cag PAI-negative infection with 15 novel cDNAs and 92 known genes being differentially expressed. H. pylori was found to change the expression of genes encoding growth factors and cytokine/chemokines and their receptors, apoptosis proteins, transcription factors and metalloprotease-disintegrin proteins (ADAMs), and tissue inhibitors of metalloproteinases. Gastric differential expression of selected known genes (amphiregulin and ADAM 10) and a novel gene (HPYR1) was confirmed in vivo in patients with H. pylori infection. Confirmation of the in vivo expression of selected genes demonstrates the usefulness of this approach for investigating pathogen-induced changes in host gene expression.


    INTRODUCTION
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

The gastric pathogen Helicobacter pylori, which adheres closely to the luminal surface of the human gastric epithelium in vivo (27), is the causative agent of active chronic gastritis and a predisposing factor for the development of both peptic ulcer disease and gastric cancer (14, 70). Molecular genetic analysis of H. pylori has shown that approximately 50 to 60% of strains have a 40-kb DNA segment called the cag pathogenicity island (PAI), which encodes a multicomponent type IV secretion system (2, 9). Whilst there is marked global variation in the frequency of cag+ H. pylori strains (71), in many populations, infection with cag+ strains has been associated with increased risk of severe gastritis (15, 52, 62, 76), peptic ulceration (12, 13, 15, 72, 76), atrophic gastritis (33, 71), and distal gastric cancer of the intestinal type (7, 51, 63, 67).

The enhanced inflammatory response induced by cag+ H. pylori strains is thought to have a key role in disease pathogenesis. In vivo gastric mucosal C-X-C chemokines are increased in those infected with cag+ strains (52, 62, 76, 77), and a major source of neutrophil chemoattractant chemokines such as interleukin-8 (IL-8) is the gastric epithelium (16). In vitro studies modelling bacterial-epithelial interactions have shown that cag+, but not strains lacking the cag PAI, induce IL-8 in gastric epithelial cells (17, 18, 60). Induction of this epithelial response, which involves mitogen-activated protein (MAP) kinase (32) and NF-kappa B activation (1, 31, 45, 61), is dependent on multiple genes throughout the cag PAI (29, 34). The cag PAI is also essential for the translocation of the bacterial protein CagA into gastric epithelial cells (4, 66) where it becomes tyrosine phosphorylated and induces cytoskeletal changes in epithelial cells (3, 4, 50, 59, 66).

The integral role of the epithelium in mucosal defense has become increasingly appreciated (30). Whilst several studies have focused on cag PAI-dependent differential expression of chemokines in gastric epithelial cells, the effects of H. pylori on the expression of other epithelial genes have not been investigated in detail. Secreted products such as chemokines, intracellular proteins, and immunologically relevant membrane proteins may all be differentially expressed in epithelial cells after microbial exposure (30). Recent studies show that even commensal intestinal bacterial flora modulate epithelial gene expression (8).

Several approaches have been adopted to detect differentially expressed genes, including subtractive hybridization (64), differential display (36, 65), serial analysis of gene expression (69), and, more recently, cDNA arrays (10, 20, 22, 26). The advantages of cDNA array technology are that it allows simultaneous expression analysis of thousands of genes to be monitored in parallel and permits identification of quantitative differences in expression of both genes of known function and novel genes and/or expressed sequence tags (ESTs) of unknown function. To date few studies have used this approach to investigate bacterially induced changes in host gene expression (21, 29). In this study the heterogeneity in gene expression profiles of a gastric cancer epithelial cell line infected with a wild-type cag PAI-positive H. pylori strain and a wild-type strain lacking the cag PAI has been examined by differential screening of cDNA arrays. The aims of the study were to identify differences in epithelial gene expression of possible relevance to the enhanced virulence of cag PAI-positive strains and to identify novel, differentially expressed genes of potential importance in the pathology of H. pylori-related disease.


    MATERIALS AND METHODS
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

Bacteria and cell culture. Wild-type H. pylori strains (cag PAI-positive NCTC 11637 and G50, which lacks the cag PAI [12]) were cultured on blood agar base number 2 (Oxoid, Basingstoke, Hampshire, United Kingdom) incorporating 7% fresh horse blood under microaerobic conditions at 37°C. Prior to use the bacteria were harvested on day 3 into RPMI 1640 medium (Gibco Life Technologies, Paisley, United Kingdom) supplemented with 10% heat-inactivated fetal calf serum (Sera Lab, Crawley, Surrey, United Kingdom) and 2 mM glutamine (Life Technologies) and were used immediately.

Kato 3 gastric epithelial cells (European Collection of Animal Cell Cultures) were routinely maintained in RPMI 1640 medium containing 10% fetal calf serum, 2 mM glutamine, and 40 µg of gentamicin/ml in 5% CO2 at 37°C. For coculture experiments Kato 3 cells were resuspended in antibiotic-free medium and cultured at 2 × 105/ml in 24-well tissue culture plates (Corning Costar, High Wycombe, Bucks, United Kingdom) with or without wild-type H. pylori strains NCTC 11637 (cag PAI positive) and G50, which lacks the cag PAI (12) at a bacterium-to-cell ratio of 50:1 as previously described (17, 18). In contrast to NCTC 11637, G50 does not stimulate IL-8 gene transcription (34) or IL-8 protein secretion (17, 18) in gastric epithelial cells. At 45 min, 3 h, and 24 h postculture, mRNA was immediately extracted from the epithelial cells using the direct mini message maker kit (R & D Systems, Abingdon, United Kingdom) and stored at -70°C. IL-8 concentrations in 24-h culture supernatants of control and H. pylori-infected cells were assayed by enzyme-linked immunosorbent assay as previously described (17, 18) to ensure the characteristic secretion of IL-8 in cells challenged with cag PAI-positive NCTC 11637. mRNA from seven independent experiments was pooled for probe preparation to minimize possible interexperimental variation.

cDNA arrays. cDNA arrays utilized were (i) a rearrayed Integrated Molecular Analysis of Genomes and Their Expression (I.M.A.G.E.) library (46,302 clones) obtained from the I.M.A.G.E. consortium (Washington University-Merck Pharm) in collaboration with the Human Genome Mapping Project, Hinxton, United Kingdom, (ii) an array containing oligodT)-primed standard spleen cDNA library clones (10, 752 clones) (Life Technologies), (iii) a custom array designed to represent 136 inflammatory genes (Glaxo Wellcome, Stevenage, Hertfordshire, United Kingdom), and (iv) a Clontech Atlas Human cDNA Expression Array 1 (588 clones). For preparation of the arrays (i to iii), each cDNA was amplified by PCR using vector-specific primers and arrayed in duplicate onto positively charged nylon membranes (Boehringer Mannheim, Lewes, East Sussex, United Kingdom) by use of a robot (Q bot) (Genetix Ltd., Christchurch, Dorset, United Kingdom). An array of control housekeeping genes, including glyceraldehyde-3-phosphate dehydrogenase (GAPDH), elongation factor 3, ribosomal L3, and beta -actin in a range of dilutions, was used as normalizing genes. The intensity of signal from the control gene arrays was used to measure the relative specific activity of probes.

The prepared cDNA arrays were initially screened with an oligonucleotide probe to assess the quantity of PCR product gridded at each position on the array. The M13F oligonucleotide dCGCCAGGGTTTTCCCAAGTCACGAC (Promega, Southampton, United Kingdom) was used for the rearrayed human I.M.A.G.E. library and inflammatory-gene array, and the SP6 oligonucleotide dTATTTAGGTGACACTATAG (Promega) was used to screen the human spleen library. The oligonucleotide annealing site vector-specfic sequence was situated within the PCR-priming sites of cDNA PCR products. The oligonucleotides were end labeled using 5 µl of 10× polynucleotide kinase buffer (700 mM Tris-HCl buffer, pH 7.6, containing 100 mM MgCl2 and 50 mM dithiothreitol) (New England Biolabs, Hitchin, Herts, United Kingdom), 1 µl of oligonucleotide (20 pmol/µl), 5 µl of [alpha -33P]dATP (3,000 Ci/mmol) (Amersham, Little Chalfont Bucks, United Kingdom), 2 µl of polynucleotide kinase (10 U/µl), and 37 µl of distilled water at 37°C for 1 h. Probes were purified using Sephadex G-25 columns, and the level of incorporation of label was measured as described above.

Prior to hybridization the arrays were preincubated in digoxigenin Easy Hyb (Boehringer Mannheim) at 45°C subsequent to hybridization for 16 h with the oligonucleotide probes (see above) at 45°C. Posthybridization arrays were washed with 6× SSC (0.9 M NaCl and 0.09 M sodium citrate containing 0.5% [wt/vol] sodium dodecyl sulfate) (1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate) at 45°C. The phosphorimages of the hybridized arrays were captured using a Storm scanner 860 (Molecular Dynamics, Chesham, Bucks, United Kingdom).

Epithelial probe preparation and hybridization. Epithelial mRNA was quantified using a UNICAM UV-visible light scanning spectrophotometer, and the integrity was assessed electrophoretically prior to radiolabeling. mRNA (1 to 2 µg) was incubated with 1 µl of d(T)15V(A, C, G)N(A, G, C, T) (1.44 µg/µl) at 70°C for 10 min prior to the addition of 4 µl of 5× reaction buffer (Life Technologies) 2 µl of 0.1 M dithiothreitol, 1 µl of deoxynucleoside triphosphate mix (1 mM dATP, dGTP, and dTTP and 0.01 mM dCTP), 1 µl of RNasin (40 U/µl) (Boehringer Mannheim), and 4 µl of [alpha -33P]dCTP (3,000 Ci/nmol) (Amersham). The mixture was incubated at 42°C for 1 min prior to addition of 2 µl of Superscript II (200 U/µl) (Life Technologies) and further incubation for 90 min. Probes were purified by passage through Sephadex G-50 columns (Pharmacia Biotech, St. Albans, Herts, United Kingdom). The incorporation rate of label was measured using a Bioscan QC-4000 (Bioscan, Washington, D.C.), and the size range of the probe was estimated electrophoretically using Tris-borate-EDTA-urea-6% polyacrylamide gel electrophoresis with radioactive size standards.

The arrays were prehybridized as described above for the oligonucleotide probe hybridizations. 33P-radiolabeled, single-stranded cDNA probes (50 µl) were equalized on counts (counts per minute) and were quenched with 5 µl of poly(A)80 (1 µg/µl), 10 µl of human Cot-1 DNA (1 µg/µl), and 435 µl of digoxigenin Easy Hyb (10 min, 100°C; and 90 min, 45°C) before hybridization to the cDNA arrays and control grids at 45°C for 3 days. Following stringent posthybridization washing in 0.1× SSC containing 0.1% Sodium dodecyl sulfate at 68°C, the phosphorimages of the hybridized arrays were scanned as done before.

Image analysis. Phosphorimages were edited using the ImageQuant package (Molecular Dynamics) and were processed using DGEnt PC software (Glaxo-Wellcome) whereby a specific intensity value was determined and assigned for each spot on an array and corresponding control genes. Each array was processed twice to provide data for PCR product loading (oligonucleotide hybridizations) and the epithelially derived sample probe hybridization for each spot. Comparisons of gene expression profiles were undertaken using the DGEnt PC software (Fig. 1). This package incorporates data from the edited ImageQuant images and allows comparison of two arrays probed with different epithelially derived samples. The comparison data were adjusted to compensate for differing probe-specific activity using control genes which included beta -actin, elongation factor 3, ribosomal L3, and GAPDH. The Clontech Atlas arrays included their own set of control genes, which were used to standardize the arrays relative to probe strength.


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  		FIG. 1.   Example of I.M.A.G.E. cDNA array hybridized with epithelial cell-derived probe from H. pylori-infected Kato 3 gastric epithelial cells (A) and a probe derived from uninfected Kato 3 cells (B). The circles highlight a differentially expressed gene.

Secondary hybridizations of cDNA putatively differentially expressed from initial screen. Clones from the I.M.A.G.E. and splenic libraries considered to be carrying potential differentially expressed cDNAs from the first hybridizations were repicked. Their inserts were amplified by PCR and arrayed as described above. The oligonucleotide and epithelial probe hybridizations were performed as before with the same batch of mRNA, and each cDNA was analyzed to verify the initial changes in gene expression. Repicked cDNA clones were sequenced using a BigDye Terminator sequencing kit (Applied Biosystems Inc., Warrington, Cheshire, United Kingdom) and analyzed on ABI 377 sequencers (Applied Biosystems Inc.).

Confirmation of differential expression of genes in vitro and in vivo. Further analysis of selected differentially expressed genes was undertaken in Kato 3 and AGS gastric epithelial cells (European Collection of Animal Cell Cultures) following stimulation with cag PAI+ G27 H. pylori and H12-5A, a cagM isogenic mutant strain (kindly provided by A. Covacci, Chiron Vaccines, Siena, Italy) which lacks the ability to induce IL-8 in Kato 3 cells (9). Total RNA was extracted at various times poststimulation using Catrimox-14 (VH Bio Ltd., Newcastle upon Tyne, United Kingdom). Following reverse transcription (RT), expression of genes of interest was analyzed by PCR as previously described (65).

The expression of genes of interest was examined in gastric biopsies of patients undergoing routine upper gastrointestinal endoscopy. All subjects provided informed consent, and the study was approved by the local research ethics committee. Total RNA was extracted from endoscopic gastric biopsies using Catrimox-14 (VH Bio Ltd.) and was treated with DNase I (Gibco Life Technologies). Purified RNA was reverse transcribed in 20 µl of solution using 0.5 µg of Random Primers (Promega) as previously described (28, 62). Expression of genes of interest was examined by semiquantitative RT-PCR using primer sequences described in Table 1. The quantity of PCR product for each gene examined was compared to the control gene GAPDH by densitometry using a UVP gel documentation system and GelBase software (GDS 5000; Ultra Violet Products, San Gabriel, Calif.) as previously described (25). PCR was also performed with a sample of the original RNA to confirm the absence of genomic DNA. The H. pylori status and cagA and ureA status of gastric biopsies were determined by biopsy urease test, histology, and RT-PCR as previously described (62, 69).

                              
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  		TABLE 1.   Primer sequences for RT-PCRs

Statistical analysis. Statistical analysis was undertaken using the two-tailed Fisher exact test and Mann-Whitney U test. A P of less than 0.05 was considered statistically significant.


    RESULTS
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

Quality control evaluation of high-density cDNA arrays. The cDNA arrays were screened initially with M13 and SP6 oligonucleotides in order to estimate the levels of individual PCR products loaded in each spot of the I.M.A.G.E. and spleen and inflammatory-cDNA arrays. A measure of the DNA loading of each spot on the array is essential prior to comparison of signals produced following hybridization with the epithelial cell-derived cDNA probes (see below).

Screening of cDNA arrays. cDNA probes prepared from mRNA extracted from Kato 3 epithelial cells following exposure to NCTC 11637 and G50 for 45 min, 3 h, and 24 h, and uninfected control Kato 3 cells (45 min, 3 h, and 24 h) were hybridized to three I.M.A.G.E. cDNA arrays, one splenic cDNA array, and one inflammatory-cDNA array, comprising 46,302, 10,752, and 136 cDNAs, respectively. A Clontech Atlas array (588 cDNAs) was also included for analysis using the 3-h and 24-h radiolabeled probes. In total, 57,778 cDNAs were screened. Following normalization against the housekeeping genes, the cDNA spot intensity values for each array were compared for the different time points. Each potential differentially expressed cDNA identified with a 1.1-fold alteration in intensity or higher in the initial screen was selected for rescreening using these criteria. Spleen clones (n = 466) and I.M.A.G.E. clones (n = 652) which potentially represented differentially expressed cDNAs were rearrayed and hybridized with cDNA probes prepared as for the first screen using the same mRNA batch. The rescreening was performed to minimize potential errors resulting from labeling, PCR, arraying, and data interpretation. Differential hybridization of 624 cDNAs was confirmed.

Characterization of differentially expressed genes found using cDNA arrays. The level of IL-8 expression in the inflammatory-cDNA arrays was examined initially, as IL-8 is known from previous studies (17, 18, 60) to be upregulated during H. pylori infection. Characteristically, IL-8 expression at 3 h was found to be 4.2-fold higher for Kato 3 cells infected with cag PAI-positive H. pylori than for cells infected with cag PAI-negative H. pylori. This confirmed that the array system and the manner in which the data had been normalized had produced the expected results for IL-8. We proceeded therefore to perform a large-scale analysis of the remainder of the differentially expressed genes.

The intrinsic redundancy of the splenic cDNA array also provided further confidence in the identification of differentially expressed genes, as several instances of detection of the same differentially hybridizing gene from independent cDNA spots were encountered (e.g., eukaryotic translation elongation factor 1 alpha 1 gene [EEF1A1] and tumor-associated protein [p23]). Other genes, such as ferritin light chain and alpha -tubulin, were identified as being differentially hybridized from both the I.M.A.G.E. and spleen libraries.

Analysis of all arrays demonstrated that the interaction of either H. pylori strain with Kato 3 epithelial cells in comparison to noninfected control cells resulted in the upregulation of 100 known genes and 34 genes of unknown function (including ESTs) and downregulation of 108 known and 12 novel genes in the epithelial cells. Furthermore, comparative analysis of gene expression between cag PAI-positive and cag PAI-negative infected Kato 3 epithelial cells indicated that 91 known genes and 15 novel cDNAs were differentially expressed by 1.3-fold or greater. Novel genes demonstrated differential expression levels ranging from 1.3 to 29. Following cag PAI-negative infection, a higher number of both known and novel cDNAs were found to be upregulated than following cag PAI-positive infection. Screening the I.M.A.G.E. (Table 2), splenic (Table 3), inflammatory- (Table 4), and Clontech Atlas (Table 5) cDNA arrays identified differences in expression of known genes following culture with cag PAI-positive and cag PAI-negative H. pylori ranging from 61.5 to 1.3. 

                              
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  		TABLE 2.   I.M.A.G.E. arraya


                              
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  		TABLE 3.   Spleen arraya


                              
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  		TABLE 4.   Inflammatory-cDNA arraya


                              
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  		TABLE 5.   Clontech Atlas arraya

Clontech Atlas and inflammatory-cDNA arrays. The Clontech Atlas arrays were included in the hybridizations with probes made from mRNA extracted from cells infected with cag PAI-positive and cag PAI-negative H. pylori at 3 and 24 h. The 3-h results revealed increased expression in 13 genes and decreased expression of 47 genes with cag PAI-positive-infected cells compared to results for cag PAI-negative-infected cells. At 24 h, 15 genes were found to be upregulated by the cag PAI-positive H. pylori strains compared to unstimulated control cells and 50 genes were found to be downregulated (data not shown).

The inflammatory-cDNA arrays which were included in every hybridization contained many cytokines, cytokine receptors, and inflammatory mediators. Examples of genes expressed in uninfected Kato 3 gastric epithelial cells and their relative intensity values are described in Table 6. Levels of expression ranged from 29 (oncostatin M) to 641 (tissue inhibitor of metalloproteinase 1 [TIMP-1]) relative grey level units. Probes made from H. pylori-infected Kato 3 cells hybridized to several other genes not expressed in unstimulated Kato 3 cells, including CD55, CD68, CD138, JAG1, IL-17, IL-3 alpha  and beta  receptors, IL-4 receptor, IL-9 receptor, IL-13 receptor, BAX, BCL2, VEGF, and several members of the ADAMs (a disintegrin and metalloprotease) family. Compared to uninfected controls, 32 genes were upregulated and 25 genes were downregulated by cag PAI-positive H. pylori infection over the time course. Similar analysis following cag PAI-negative H. pylori infection demonstrated that 24 genes were upregulated and that 17 genes were downregulated throughout the time course. Thirty-one genes were more highly expressed and 22 genes were less expressed by cag PAI-positive infection than by cag PAI-negative infection (Table 4).

                              
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  		TABLE 6.   Expressed inflammatory mediator genes in control uninfected Kato 3 cells after culture for 24 ha

In vivo verification of differentially expressed genes. The expression of three genes found to be upregulated in gastric epithelial cells following infection with H. pylori was further examined in gastric biopsies of patients with and without H. pylori infection by semiquantitative RT-PCR. In the antral mucosa, increased mRNA transcripts for ADAM 10 (Fig. 2A), amphiregulin (Fig. 2B), and a novel gene in the I.M.A.G.E. library upregulated at 0.75 h following cag PAI-positive infection (H. pylori responsive 1 gene HPYR1, accession no. AF200341) (Fig. 2C) were present in H. pylori-infected patients but were infrequently observed in H. pylori-negative patients. HPYR1 transcripts were observed in the antral mucosa of 42% of patients with cagA-positive infection (n = 24) but in only 15% of patients infected with cagA-negative strains (n = 13) and 16% of uninfected subjects (n = 19). In contrast, ADAM 10 mRNA expression was observed significantly (P < 0.05) more frequently in those with cagA-negative H. pylori infection (n = 14) than in uninfected patients (n = 19) or those with cagA-positive infection (n = 28) (Table 7). Amphiregulin mRNA expression in antral (n = 59) and corpus (n = 39) biopsies was significantly increased (P < 0.05) in the antrum but not in the corpus in H. pylori infection (Fig. 3). Levels of amphiregulin mRNA were similarly increased in both cagA-positive and cagA-negative infection.




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  		FIG. 2.   Representative RT-PCR with primers for ADAM 10 (A), amphiregulin (AR) (B), and HPYR1 (C) in gastric antral biopsy samples. G3PDH is the control gene GAPDH. Lane L, 100-bp ladder; lanes 1 to 3, H. pylori negative normal mucosa; lanes 4 to 6, mucosa from patients with cagA-negative H. pylori; lanes 7 to 9, mucosa from patients with cagA-positive H. pylori; lane 10, positive control (H. pylori-stimulated Kato 3 gastric epithelial cells); lane 11, negative control. Hp, H. pylori.

                              
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  		TABLE 7.   Number of patients demonstrating expression of ADAM 10 and HPYR1 transcripts in human gastric antral mucosaa



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  		FIG. 3.   Semiquantitative analysis of amphiregulin (AR) in antral and corpus mucosa of H. pylori-positive (Hp +ve) and -negative (Hp-ve) patients. Levels of amphiregulin relative to GAPDH (G3PDH) are indicated on the y axis. H. pylori-positive patients had significantly greater (P < 0.05) amphiregulin mRNA expression in the antrum than did H. pylori-negative patients with normal mucosa. NS, not statistically significant.

Expression kinetics and cag PAI specificity of HPYR1. As HPYR1 was confirmed to be upregulated in vivo in the antral mucosa of patients infected with cagA+ strains, further in vitro studies were undertaken to investigate the kinetics of expression of this gene in H. pylori-stimulated Kato 3 and AGS gastric epithelial cells. As wild-type strains with and without the cag PAI were used for the cDNA array hybridization experiments, the cag PAI specificity of the HPYR1 response was examined using a cagM isogenic mutant strain, H12-5A, and the cag PAI-positive parental strain G27. Increased HPYR1 mRNA expression was observed at 45 min postinfection with the cag PAI-positive G27 strain in both Kato 3 cells and AGS cells. At 3 and 6 h post-G27 infection, HPYR1 mRNA was evident in Kato 3 cells but was not detected at 24 h poststimulation (Fig. 4). No increase in HPYR1 mRNA expression relative to unstimulated control cells was observed in cells cultured with the cagM isogenic mutant strain H12-5A (Fig. 4), suggesting that upregulation of HPYR1 in gastric epithelial cells was cag PAI dependent. No amplification products were evident in PCR analysis of non-reverse transcribed controls, confirming the absence of DNA contamination.


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  		FIG. 4.   Representative RT-PCR for HPYR1 and GAPDH (G3PDH) in Kato 3 gastric epithelial cells following coculture with cag PAI-positive strain G27 and an isogenic Delta cagM mutant. Cells were harvested for RT-PCR analysis at 45 min and 3, 6, and 24 h postinfection. Lane L, 100-bp ladder; lane -ve, negative.


    DISCUSSION
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

High-density cDNA array technology permits the simultaneous, multiple screening of tens of thousands of genes to identify host gene expression patterns and identify new, previously uncharacterized, disease-associated genes. We have used this approach to examine in vitro the transcriptional response of gastric epithelial cells to H. pylori. The splenic and custom inflammatory-cDNA arrays used in this study have identified many previously unknown H. pylori-induced immune-response genes in epithelial cells. The I.M.A.G.E. library similarly has allowed identification of multiple known genes and novel ESTs. The Clontech Atlas array, which includes genes considered to be important in human disease, provided a further screen for candidate epithelial response genes.

One advantage of the I.M.A.G.E. and splenic arrays was that a large number of cDNAs (circa 58,000) could be screened simultaneously. On the primary screen, approximately 1.9% of genes were identified as differentially expressed. These results strongly support the earlier observations of Eckmann et al. (21) that the intestinal epithelial response to bacterial pathogens is very specific and narrow. Interestingly, in the latter study mRNA expression analysis of ca. 4,300 genes in intestinal epithelial cells following infection with Salmonella demonstrated that immune regulatory genes such as IL-17 and oncostatin M were upregulated in a manner similar to that observed in the present study. However, the study of Eckmann et al. (21) reported only on genes whose expression was upregulated. It is clear from the present study that infection of gastric epithelial cells with H. pylori results also in the downregulation of the expression of multiple host epithelial genes. In addition, many genes showed marked temporal changes in expression, being both upregulated and downregulated relative to uninfected control epithelial cells at various time points postinfection.

Several hundred differentially expressed genes were identified that exhibited changes in expression levels following infection with H. pylori over the three time points studied. Infection with both strains induced marked temporal changes in gene expression, emphasizing the importance of longitudinal studies in gene expression profiling using in vitro model systems. This approach has the additional advantage of allowing cluster and principal component analysis (22) of differentially expressed epithelial genes to identify coregulated genes.

As cag PAI-positive strains activate NF-kappa B (31, 45, 61) and MAP kinase pathways (32, 48), it is not surprising that the transcriptional response of gastric epithelial cells induced by cag PAI-positive H. pylori markedly differed from that induced by the cag PAI-negative strain. Understanding the differences in the transcriptional response of gastric epithelial cells to H. pylori strains with different virulence characteristics should shed light on bacterial pathogenicity. This study focused on differences in gene expression induced by the wild-type cag PAI-positive and -negative strains, as such differences may be potentially relevant to the more severe clinical outcome associated with infection with cag PAI-positive strains. Furthermore, a focused approach is essential, given the large number of data generated from screening ca. 58,000 arrayed cDNA sequences. Known genes demonstrating early (0.75 to 3 h) differential expression included several genes involved in cell signaling pathways, which were decreased in cag PAI-positive-infected epithelial cells such as MAP kinase kinase 3, tyrosine protein kinase cak, guanine nucleotide binding protein G alpha  subunit, and related transducin beta -2. In contrast, cag PAI-positive strains induced an early increase in expression of several genes involved in transcriptional regulation, such as MAD-3, the I-kappa beta inhibitor of NF-kappa B and TCS-22. At 24 h increased expression of TFIIIA was observed with cag PAI-positive-H. pylori-infected cells. This protein is involved in initiation of transcription of 5S ribosomal RNA genes. In contrast, a substantial decrease in expression of the transcription factor BTF3, which complexes with RNA polymerase II (81), was observed.

The activation of NF-kappa B has been linked with suppression of apoptosis via NF-kappa B-controlled antiapoptotic genes (5, 74). In H. pylori infection both gastric epithelial expression and activity of NF-kappa B (68) and apoptosis (44) are increased. Interestingly, infection with cag PAI-positive strains has been associated with a reduced apoptotic index and higher gastric epithelial cell proliferation than that found for cag PAI-negative infections (53). Expression of the cell regulatory protein cyclin D1 was decreased following infection with the cag PAI-positive strain, and differential expression of genes involved in apoptosis such as AAC-11 and Bclx and also of redox-related genes such as NADPH-cytochrome P450 reductase was observed. p53-induced apoptosis has recently been linked to early transcriptional induction of redox-related genes involved in oxidative damage to mitochondria (54). Infection of gastric epithelial cells with the cag PAI-positive strain induced a 12-fold increase in the apoptosis inhibitor AAC-11 at 45 min but decreased expression at 3 and 24 h. AAC-11 inhibits apoptosis in cervical cancer cells, and transfection studies demonstrate that AAC-11 expression is associated with loss of TIMP-2 expression (35). Interestingly, in the present study, changes in expression of TIMP-2 and TIMP-1 (Table 4) were inversely related to that of AAC-11 (Table 2). TIMP-1 and TIMP-2 are expressed in approximately 50% of gastric cancers (47). Further studies to analyze expression of AAC-11, TIMP-1, and TIMP-2 in vivo in relation to H. pylori infection and gastric cancer will be of interest.

Infection with cag PAI-positive strains was associated with early decreased expression of genes coding for cellular regulatory proteins, such as elongin B; genes coding for ribosomal proteins Sm of protein G and protein L28; and also genes involved in RNA processing, e.g., alpha -NAC and poly(A) binding protein. Elongin B, a ubiquitin-like protein, is part of the multifunctional regulatory elongin BC complex. This complex, when bound to the von Hippel-Lindau tumor suppressor gene, is thought to play an important role in negatively regulating hypoxia-inducible proteins by promoting degradation of HIF1alpha (58) and also the stability of the suppressor of cytokine signaling 1 (SOCS-1) proteins by promoting their degradation (80). As elongin B is a component of the von Hippel-Lindau ubiquitin-protein ligase (E3) complex, there is the potential that it competes for components of other E3s, such as Rbx1/ROC1, that are found in the I-kappa B SCFHOS complex, thereby reducing the activity of the latter. As recent studies demonstrate that nonvirulent Salmonella inhibits I-kappa B-alpha ubiquitination (49) and thus attenuates acute inflammatory responses, further investigation of the functional importance of regulatory proteins such as elongin B in epithelial responses to enteric bacteria is warranted.

At 24 h postinfection, several interesting genes were identified on the I.M.A.G.E. arrays, which demonstrated decreased expression following infection with the cag PAI-positive strain. One of these was thymosin beta 4, which has been reported to have multiple functions, including inhibition of actin polymerization (19) and promotion of wound healing and angiogenesis (38). The oxidized form of thymosin beta 4, which is known to be induced in monocytes by glucocorticoids, also acts as an anti-inflammatory agent attentuating neutrophil-associated inflammatory processes (79). The preferential expression of thymosin beta 4 and also of L apoferritin, a known antioxidant response gene (55), at 24 h following infection with the cag PAI-negative strain suggests that these strains may have the potential to attenuate gastric inflammatory responses and protect against mucosal damage. The upregulation of L apoferritin is likely to protect against oxidative damage by enhancing capacity for iron storage. Interestingly, serum ferritin levels are reduced in H. pylori infection, but the relation with bacterial cag PAI status has not been investigated (42). An additional gene of interest was the membrane protein E16, the expression of which was also increased at 24 h following cag PAI-negative infection. The encoded protein of this gene heterodimerizes with CD98 to function as a cationic amino acid transporter (39). Such transporters may regulate the availability of arginine in epithelial cells and thus regulate cellular nitric oxide production (43). Further investigation of the expression of these potentially protective host response genes in vivo in relation to H. pylori infection will be important.

Hybridization of the inflammatory-cDNA arrays with epithelially derived probes demonstrated that infection with the cag PAI-positive strain resulted in increased expression of several genes involved in immune regulation. Differential expression of transcripts encoding several cvtokines (e.g., IL-8, IL-7, IL-17, and oncostatin M), cytokine receptors (tumor necrosis factor receptors I and II, granulocyte colony-stimulating factor receptor, and alpha and beta interferon receptors), and members of the ADAMs family, which have an important function in cytokine, cytokine receptor, and growth factor shedding (75), was observed. Recent studies have confirmed that IL-17 is upregulated in the gastric mucosa in H. pylori infection (37). Analysis of expression of the identified genes in patients with H. pylori infection of defined cag PAI status is required to confirm differential expression in vivo.

In this study two known genes, amphiregulin and ADAM 10, and one gene of unknown function (HPYR1) were chosen for further analysis of expression in vivo in the gastric mucosa. Amphiregulin is a member of the epidermal growth factor family, which has a mitogenic effect on epithelial cells. It is present in parietal cells (46) and is overexpressed in gastric carcinomas (11). Previous studies have indicated that soluble products of H. pylori induce amphiregulin in MKN28 gastric epithelial cells (56). Our in vivo results confirm that H. pylori infection is associated with upregulation of amphiregulin mRNA expression in the antral mucosa. Whilst a low level of differential expression of amphiregulin following infection with cag PAI-positive and -negative strains was observed in the cDNA arrays, in vivo similar expression levels were observed in patients infected with cag PAI-positive and -negative strains. This confirms earlier in vitro studies that induction of amphiregulin in gastric epithelial cells by H. pylori was independent of a functional cag PAI (56).

The inflammatory-cDNA arrays showed that H. pylori induced temporal changes in gene expression of four members of the ADAMs family of membrane proteins. This recently identified gene family has important functions in the release of cell surface molecules and cell-cell and cell-extracellular matrix interactions (6, 75). ADAM 10, which is expressed in a range of hematological malignancies (73) and in prostate cancer cell lines (40), has both collagenase type IV activity (41) and also cleaves pro-TNF alpha (TNF-alpha ) to the soluble form (57). Our in vivo results confirm that ADAM 10 mRNA expression is increased in H. pylori infection in the gastric mucosa; interestingly, expression was more frequent in patients infected with cag PAI-negative strains. Recent studies confirm that levels of transcripts coding for ADAM 17, also known as TNF-alpha converting enzyme, are also increased in gastric H. pylori infection (78). Further studies are required to assess the functional importance of ADAM proteins in H. pylori-induced gastric pathology.

Another advantage of screening the I.M.A.G.E. and spleen arrays is that, following comparison with nucleotide databases (GenBank and EMBL), new expression data for novel and previously uncharacterized genes, ESTs, have been obtained. Further analysis of these genes is beyond the scope of the present study. However, we investigated in vivo expression of one gene, HPYR1, which was upregulated at 0.75 h after exposure to cag PAI-positive strains. Our in vivo data confirm an association of HPYR1 expression in patients with cag PAI-positive H. pylori infection. Furthermore, our additional in vitro studies with an isogenic cagM mutant strain demonstrated that the induction of HPYR1 is dependent on a functional cag PAI and not on the presence or expression of genes at other loci. The sequence used to design the HPYR1 PCR oligonucleotide-specific primer pairs was from one long contiguous sequence of approximately 1.2 kb found on a BAC clone (543J1) from chromosome 8q24. This sequence may represent the 3' untranslated region of the transcript, as no open reading frame was identified and a MER68A repetitive sequence element was located 3' to the amplification region. Alternatively, it may represent a nontranslated RNA transcript (23, 24). It obviously represents a transcript sequence, as RT-negative controls consistently gave negative results, indicating an absence of contaminating genomic DNA.

Further investigation of the previously uncharacterized genes that indicate differential expression in vivo is in progress. As the initial screen used a gastric cancer epithelial cell line, some differentially expressed genes have been found to be tumor specific (R. Stephens et al., unpublished data). Further investigation of these ESTs could lead to the identification of new genes relevant not only to bacterially induced enteric disease but also to gastric neoplasia. The application of cluster analysis and principal component analysis (22) of the temporal data sets obtained in this study may also provide insight into the function of novel genes.


    ACKNOWLEDGMENTS

This study was undertaken with the financial support of Yorkshire Cancer Research and the European Commission (contract ICA4-CT-1999-10010).

We thank the staff of the Centre for Digestive Diseases at Leeds General Infirmary for their cooperation.


    FOOTNOTES

* Corresponding author. Mailing address: Level 7, Clinical Sciences Building, St. James's University Hospital, Leeds LS9 7TF, United Kingdom. Phone: 44-113-2065267. Fax: 44-113-2429722. E-mail: MSJJC{at}stjames.leeds.ac.uk.

Editor:   E. I. Tuomanen


    REFERENCES
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

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Infection and Immunity, November 2001, p. 6970-6980, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.6970-6980.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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