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Previous Article | Next Article 
Infection and Immunity, October 2002, p. 5346-5354, Vol. 70, No. 10
0019-9567/02/$04.00+0 DOI: 10.1128/IAI.70.10.5346-5354.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
Sir William Dunn School of Pathology, Oxford University, Oxford, OX1 3RE,1 Institute of Molecular Medicine and Department of Paediatrics, John Radcliffe Hospital, Oxford University, Oxford, OX3 9DU, United Kingdom,3 Department of Molecular Genetics, Maastricht University, 229ER, Maastricht, The Netherlands,2 Department of Molecular Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, Tokyo 153, Japan4
Received 28 January 2002/ Returned for modification 10 April 2002/ Accepted 20 June 2002
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ABSTRACT |
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) play a key role in the pathogenesis of invasive meningococcal infections. The roles of two pattern recognition molecules, the M scavenger receptor (SR-A) and Toll-like receptor 4 (TLR-4), have been investigated using bone marrow culture-derived M (BMM). Surprisingly, a comparison of BMM from wild-type and SR-A knockout (SR-A-/-) mice showed that nonopsonic phagocytosis of meningococci was mediated almost exclusively via SR-A. Previous studies have demonstrated only a partial involvement of the receptor in the uptake of other bacteria, such as Escherichia coli. Interestingly, we also show that lipopolysaccharide (LPS) was not the ligand for the receptor on these organisms. Further study of the downstream events of SR-A-mediated ingestion of Neisseria meningitidis demonstrated that SR-A was not required for cytokine production. To determine the bacterial and host factors required to stimulate M activation, we examined TLR-4-deficient M from C3H/HeJ mice and LPS-deficient meningococci. TLR-4-deficient cells elaborated reduced amounts of tumor necrosis factor alpha, interleukin-12 (IL-12), and IL-10, even though ingestion via SR-A was unaffected in these cells. Similarly, although there was no change in SR-A-mediated ingestion of LPS-deficient meningococci, the mutant failed to stimulate a M-dependent cytokine response. Thus, we show that M SR-A mediates opsonin-independent uptake of N. meningitidis independently of lipid A and that this activity is uncoupled from the M secretion of proinflammatory cytokines, which provides a basis for further investigation of the role of this receptor in meningococcal disease in humans. |
INTRODUCTION |
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Many aspects of the host response to meningococcal infection remain obscure. Most studies have focused on the protective (bactericidal) activity mediated by antibody and complement (26, 45) and the inflammatory responses that result from circulating endotoxin, a correlate of disease severity (1). Large numbers of neutrophils are recruited to sites of acute infection, and epithelial cells in the nasopharynx and choroid plexus may provide barriers to invasion. Bacterial pili and Opa/Opc proteins are required for attachment to these cells (40, 42), and meningococci, sensitive to uptake by neutrophils, are potent activators of leukocytes in vitro (14, 32). However, we know little of factors governing the initial host-microbial interactions which result in clearance, colonization, or spread from the nasopharynx and why some individuals progress to fulminant septicemia.
The potential role of macrophages (M) has hitherto received little attention aside from their proinflammatory response following exposure to meningococcal endotoxin (2, 21, 28). Monocytes are readily mobilized to sites of infection for local activation (8), and resident M are present in the nasopharynx and systemically, including at the blood-cerebrospinal fluid (CSF) interface (9). Meningococci were first observed within M in CSF from patients (3, 18) and have been detected in M-like cells following experimental infection of explanted human rhinopharyngeal mucosa (30). Thus, M may be important during the initial and subsequent interactions between host and meningococci and during dissemination, as well as in the generation of acquired immunity.
M express several surface molecules that aid in recognition of and response to microorganisms, including the pattern recognition molecules (PRM), such as CD14, the toll-like receptors (TLR), and the scavenger receptors (SR), which are able to recognize conserved motifs on pathogen surfaces directly (5, 20, 24). N. meningitidis binds to M in the absence of serum, but the receptors have not been identified (31). CD14 and the class A M SR (SR-A) (24), a trimeric transmembrane glycoprotein, are candidate receptors, as they bind lipopolysaccharide (LPS), either directly or through LPS-binding protein. CD14 is highly expressed on monocytes and down-regulated on tissue M (20, 47), whereas SR-A, present on resident M, is absent on monocytes (10, 16).
In vivo studies have shown that both TLR-4 and SR-A function in defense against pathogens and endotoxic shock, though their roles are contrasting. CD14- and TLR-4-deficient animals are resistant to endotoxic shock and infection by gram-negative organisms (13), supporting their roles in the generation of inflammation. Conversely, SR-A-/- animals have increased susceptibility to gram-positive infection in vivo (34, 37), and Mycobacterium bovis BCG-primed SR-A-/- mice are more sensitive to LPS (12), suggesting a role for SR-A in limiting endotoxemia. Using SR-A-/- mice, it was shown that the direct binding and uptake of various gram-positive and gram-negative organisms are mediated in part by SR-A+/+ M in vitro (25). Therefore, we examined the role of SR-A in nonopsonic phagocytosis of N. meningitidis and cytokine secretion following bacterial contact with M.
We show that N. meningitidis binds to bone marrow culture-derived M (BMM) almost exclusively through SR-A. Although meningococcal entry into M resulted in bacterial killing and cytokine production, we were unable to demonstrate any SR-A-mediated contribution to the induction of proinflammatory cytokines. Conversely, TLR-4 mutant M were unable to signal cytokine release, but phagocytosis was unaltered in these cells. Examination of bacterial components required for M cytokine secretion showed that the ligand for SR-A-mediated phagocytosis was not lipid A, although it was required for M activation. Thus, we demonstrate in vitro that ingestion of meningococci by M, mediated by SR-A, can be dissociated from the production of inflammatory cytokines. These findings provide a critical insight upon which to pursue subsequent in vivo studies of the complex interplay of meningococci and host cells in the pathogenesis of invasive N. meningitidis disease.
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MATERIALS AND METHODS |
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Animals. SR-A-/- mice were produced as described previously (35). These cells do not express any of the SR-A isforms. C3H/HeN and C3H/HeJ mice were purchased from Harlan-Olac. SR-A-/-, 129/ICR (wild-type [WT] controls), and C3H/HeN and C3H/HeJ animals of the same sex were used at 8 to 12 weeks of age. All animals used were handled in accordance with guidelines issued by the Home Office, United Kingdom.
Cell isolation and culture.
M were obtained as described previously (25). BMM were cultured in RPMI supplemented with 10% fetal calf serum (FCS) (Sigma, Reading, United Kingdom) and 15% L-cell conditioned medium; human cells were cultured in X-VIVO with 2% autologous human serum. CHO K1 cells (a nonphagocytic hamster ovary cell line) were routinely cultured in Ham's F-12 medium supplemented with 10% FCS. CHO cells stably transfected with SR-AII were produced as described previously (10) and maintained in Ham's F-12 medium containing 3% lipoprotein-deficient FCS, 250 µmol of mevalonate/liter, 40 µmol of mevastatin/liter, and 3 µg of acetylated low-density lipoprotein/ml (7). OPTIMEM, a serumless medium, was used for all assays.
Bacterial culture and labeling.
N. meningitidis MC58 (40), 44/76 (15), capsule mutants (MC58 
3) (44), 44/76lpxA (38), and the Centers for Disease Control and Prevention reference strains, F8238 and C11, were treated as described previously (40). For fluorescent labeling, N. meningitidis cells were fixed in 70% ethanol and labeled with RdGnX according to the manufacturer's protocol.
Association of ethanol-fixed bacteria with M.
For flow cytometry, M were incubated for 2 h at 37°C with RdGnX-labeled bacteria as described previously (25). As required, cells were preincubated for 30 min with inhibitor, which was retained throughout the assay. Poly(I) and poly(C) were used at 50 µg/ml, cytochalasin D was used at 2 µM, and 2F8 and CAMPATH IG were used at 15 µg/ml. Cells for flow cytometry were washed and harvested with phosphate-buffered saline (PBS) containing 10 mM EDTA and 4 mg of lidocaine-HCl/ml before fixation with 4% formaldehyde. Fluorescence was analyzed on a FACScan (Becton Dickinson, Mountain View, Calif.) using the FL-1 or FL-2 photomultiplier where appropriate, and the results were analyzed with CellQuest software. The mean fluorescence of unloaded control cells was subtracted from the mean fluorescence of each assay condition, and the average was determined. The results are representative of at least three independent experiments. The statistical significance of the results was determined using the paired Student t test, and significance was tested at the 95% confidence level (P 
0.05). The abovementioned assay measures the total association or uptake of bacteria to cells and does not distinguish between binding and ingestion. Attempts to modify the flow cytometric assay to distinguish between binding and internalization have been described elsewhere (25). Cells for fluorescence microscopy were not harvested, but PBS was added to the M and the cells were observed using a Zeiss Axiovert 25 CTL microscope equipped with a 50-W mercury vapor lamp fitted with standard filter sets for fluorescein isothiocyanate and rhodamine fluorescence. The fields chosen for photography were representative of the whole well.
ELISAs and intracellular cytokine assays.
M were incubated with N. meningitidis, and supernatants or cells were tested using cytokine ELISAs or intracellular tumor necrosis factor alpha (TNF-
) assays according to the manufacturer's protocol. One microgram of LPS per milliliter was used as a positive control. Each condition was examined in quadruplicate for cytokine ELISA.
Uptake of live N. meningitidis by M.
Assays were performed as described previously (40, 41). Briefly, bacteria were harvested after 12 to 18 h of growth on brain heart infusion agar plates and resuspended in PBS. To remove large aggregates, the bacteria were centrifuged at 85 x g for 1 min and then counted before addition to M cultures at the required concentration for 2 h at 37°C. M were washed to remove unbound organisms, and the total number of CFU was estimated after addition of saponin (1% final concentration). The number of cell-associated bacteria was determined after correction for attachment to exposed bacteriological plastic, as described previously (40, 41). All bacteria were equally resistant to treatment with 1% saponin. To determine the number of internalized bacteria and to examine their killing by M over time, M were incubated with bacteria as described above and then washed, and fresh culture medium containing 200 µg of gentamicin/ml was added to each well for 1.5 h to eliminate extracellular bacteria. A control for gentamicin killing was included in each assay by incubating bacteria alone with the antibiotic, which killed all the organisms. Following gentamicin treatment, the M were washed and lysed with saponin and the number of CFU was estimated as described above when examining intracellular bacteria only, or the cells were washed and placed in culture medium, and at each time point of a time course they were lysed and the number of bacteria was determined. Each variable was assayed in triplicate, and each experiment was repeated at least three times. The statistical significance of the results was determined using the paired Student t test, and significance was tested at the 95% confidence level (P 0.05).
EM.
M were plated on 35-mm-diameter bacteriological-plastic petri dishes and incubated with live MC58 bacteria. At various times, cells were fixed for electron microscopy (EM) by standard protocols. Sections were examined on a Zeiss Omega 912 electron microscope (LEO Electron Microscope Ltd., Oberkochen, Germany), and digital images were captured with the integrated Soft Imaging Software (Munster, Germany) image analysis package.
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RESULTS |
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deficient in SR-A display markedly reduced ingestion of ethanol-fixed N. meningitidis.
To assess the roles of M and SR-A in interaction with meningococci, we compared bacterial phagocytosis by WT and SR-A-/- BMM. Using this method, SR-A-deficient M had an approximately 30 to 55% reduction in the ingestion of E. coli (25) but were still able to internalize organisms through other receptors. WT and SR-A-/- M were incubated with 20 ethanol-fixed RdGnX-labeled N. meningitidis serogroup A (strain F8238) bacteria per cell for 2 h in the absence of serum. Fluorescence microscopy (Fig. 1) and flow cytometry (Fig. 2a) demonstrated that uptake of F8238 by WT M was heterogeneous, but most cells ingested bacteria. In contrast, surprisingly, the binding of meningococci to SR-A-/- BMM was minimal. Comparison of fluorescence showed that SR-A-/- M associated with 88 to 94% fewer N. meningitidis bacteria than WT cells (Fig. 2b), even over a range of bacterial doses (not shown). Poly(I) (Fig. 2b), a general SR inhibitor, and 2F8 (not shown), an anti-SR-A monoclonal antibody, blocked N. meningitidis association to WT M, confirming specific binding through SR-A, whereas mannan, an inhibitor of another PRM, the mannose receptor (Fig. 2b), had no effect. The presence of opsonizing serum increased uptake via SR-A-/- M but not to WT levels, and uptake by WT cells was still partially blocked by 2F8 (not shown). EM confirmed the presence of intracellular bacteria within phagosomal membranes (Fig. 3).
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recognition of N. meningitidis via SR-A using laboratory-derived or natural mutants showed that the presence or absence of pili, capsule, or Opa or Opc protein did not alter SR-A-mediated binding (not shown). Also, binding was conserved over the genus Neisseria, as Neisseria gonorrhoeae, Neisseria lactamica, Neisseria mucosa, and Neisseria sicca all showed dramatic reductions (85 to 90%) in their associations with SR-A-/- M (not shown) compared with WT cells. We used N. meningitidis strain MC58 in further studies because of the public health importance of serogroup B infections.
We were unable to test the binding of N. meningitidis to human SR-A expressed on M, as specific inhibitors for human SR-A are not available. However, when we examined MC58 binding by all polyanion-sensitive SR in human M, the uptake of MC58 by primary human monocyte-derived M in the presence of poly(I) was blocked 69% ± 9% (not shown) whereas poly(C), a cognate non-SR ligand, had no effect. Also, CHO cells stably transfected with human SR-A associated with significantly more ethanol-fixed RdGnX-labeled MC58 (RdGnX-MC58) bacteria than control untransfected CHO K1 cells. Therefore, SR are also dominant receptors in nonopsonic meningococcal uptake by human M, although they do express other molecules that may bind N. meningitidis, and human SR-A is able to bind to N. meningitidis.
M ingest live N. meningitidis bacteria through SR-A.
To confirm that this specific interaction was not an artifact of bacterial fixation, BMM were incubated with 50 live MC58 bacteria per cell for 2 h, and bacterial association was determined by colony counting (Fig. 2c). SR-A-/- M consistently associated with 90% fewer bacteria, including F8238 and C11 (not shown), than did WT M. Poly(I) and 2F8 inhibited binding to WT M, but an isotype-matched control, CAMPATH IG, had no effect (Fig. 2c). Gentamicin was used in assays to measure bacterial internalization (36), and the difference in uptake between WT and SR-A M was maintained (not shown).
N. meningitidis inside phagosomes is killed by M.
Our system provides a good model for the examination of the downstream cellular events following SR-A ligation. It also can provide further insight into the role of M in meningococcal disease, and for this reason, the uptake of live N. meningitidis bacteria by BMM from SR-A-/- and WT mice was followed over time using EM. BMM were incubated with 150 live MC58 bacteria per cell for various times before fixation. As observed by flow cytometry and fluorescence microscopy, most SR-A-/- M contained no bacteria, although some organisms were detected close to the cell surface as classical diplococci with numerous membrane blebs (Fig. 3, upper left). By 30 min, WT M contained many organisms closely apposed to the phagosome membrane (Fig. 3, upper right), confirming that the organisms were ingested by the cells. Following the ingested bacteria over time, EM revealed that the phagosomes became more spacious with time (Fig. 3, lower left) and by 2 h were filled with amorphous material likely of bacterial origin. Intracellular organisms lacked membrane blebs and now appeared as single bacteria. After 5 h, only bacterial remnants remained in the phagosomes (Fig. 3, lower right), showing that the M are able to kill the ingested MC58. Colony assays confirmed that SR-A-/- M took up fewer living organisms (Table 1).
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cytokine production is independent of SR-A.
Cytokines commonly produced by M and previously detected in the circulation or CSF of patients infected with N. meningitidis (1) were assayed. TNF- was detected within 2 h of infection in vitro, peaking at 8 to 16 h (Fig. 4a). Secretion of interleukin-6 (IL-6), IL-12, and IL-10 coincided, or closely followed, that of TNF- (Fig. 4a). We tested whether one or more factors present in the bacterial culture supernatant were responsible for the induction of cytokines. The bacterial suspension was filtered and centrifuged to remove all particulate matter before incubation with the M for 24 h. Some secretion was observed; however, the amount of cytokines secreted was less than that of cells treated with whole bacteria (not shown).
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(Fig. 4a), nor did they correlate with uptake. We therefore examined cytokine production by individual cells using TNF- as a model cytokine. M were incubated with MC58 in the presence of brefeldin A for 5 h (Fig. 4b). SR-A-/- M did not ingest many bacteria, but most of the cell population produced levels of TNF- comparable to that of WT cells (Fig. 4b). Similarly, when the BMM were incubated with live MC58 bacteria for 5 h, only a small decrease in TNF- expression by SR-A-/- M was detected after exposure to the live bacteria (not shown). Taken together, these data confirm that to a considerable extent, ingestion via SR-A can be dissociated from the M response to N. meningitidis.
Lipid A is not the ligand for SR-A on N. meningitidis but is required for cytokine induction.
The data described above suggest that ingestion of meningococci via SR-A is not necessary for M activation and that it requires other receptors. We wanted to determine the components required for the induction of the M response to N. meningitidis, both on the cell and on the bacteria. Lipid A is a known activator of M, and we therefore examined a well-characterized mutant strain of N. meningitidis totally lacking LPS (44/76lpxA) (33). Also, the lipid A moiety has been shown to bind SR-A (11) and was the best candidate ligand for the specific binding we observed, especially as the presence or absence of capsule, pili, or Opa and Opc proteins did not alter SR-A-mediated binding of meningococci (see above). Surprisingly, as with the WT strain of N. meningitidis (44/76), SR-A-/- M incubated with 44/76lpxA associated with 96% fewer bacteria than WT M (Fig. 5a), showing that LPS was not responsible for specific SR-A binding in this system. Compared with the control strain of N. meningitidis, more 44/76lpxA organisms were taken up by both M populations and 2F8 blocked most bacterial binding by WT M, while CAMPATH IG had no effect (not shown). Thus, these data show that another ligand(s) exists for SR-A on the bacterial cell surface.
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were incubated with ethanol-fixed 44/76 or 44/76lpxA bacteria for 24 h before the culture medium was analyzed by ELISA (Fig. 5b). Similar to MC58, 44/76 bacteria induced TNF-, IL-6, and IL-12 production by WT and SR-A-/- cells. However, the induction of proinflammatory cytokines by the lipid A-deficient mutant, 44/76lpxA, was reduced (Fig. 5b) even over a range of bacterial doses (not shown). TNF- was decreased by 76 to 87%, and IL-12 was decreased by 89 to 95%. The IL-6 concentration in the culture supernatant was also reduced, although only by 41 to 59%, implying a greater contribution from other surface molecules in IL-6 release.
M require TLR-4 for activation following exposure to N. meningitidis.
To determine what cellular factors were required for cytokine production following uptake via SR-A, we examined TLR-4-deficient M. It has been shown that TLR-4 is required for proinflammatory cytokine release in response to LPS (23). We followed cytokine production after uptake of N. meningitidis through SR-A by M isolated from C3H/HeN and C3H/HeJ animals (Fig. 6). Analysis of N. meningitidis uptake by C3H/HeN and C3H/HeJ BMM using flow cytometry showed that C3H/HeN M associated with more N. meningitidis bacteria than C3H/HeJ cells, but the difference was not significant (Fig. 6a) (P > 0.05). Both 2F8 and poly(I) blocked 80 to 90% of bacterial uptake by both types of M, confirming that the contribution of SR-A to N. meningitidis uptake was maintained in these strains.
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was examined when the cells were challenged for 24 h with E. coli LPS, as a positive control, or ethanol-fixed MC58. The secretion of TNF-, IL-10, and IL-12 was measured by ELISA (Fig. 6b). C3H/HeJ M had a greatly reduced response to E. coli LPS. The low levels of TNF- detected (Fig. 6b) may be due to contamination of the LPS with other bacterial products, such as peptidoglycan, that elicit cytokine responses via other TLRs. Following incubation with MC58, both C3H/HeN and C3H/HeJ M were able to secrete TNF-, IL-10, and IL-12, but the concentrations produced by C3H/HeJ cells were markedly reduced (Fig. 6b). Incubation with 2F8 to reduce ingestion via SR-A had no effect on cytokine release (not shown). These data show that TLR-4 is a major receptor for cytokine release by M and that ingestion of the bacteria via SR-A is not required for M activation. |
DISCUSSION |
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PRM, TLR-4 and SR-A, using M obtained from animals selectively deficient for either receptor function. The main findings are that SR-A is a major PRM for N. meningitidis uptake but is not required for cytokine release. Conversely, TLR-4 was not required for meningococcal entry into M, but cells lacking the receptor were unable to secrete high levels of cytokine following exposure to N. meningitidis. Although LPS was required for M cytokine production, it was not the ligand for SR-A. Thus, uptake of meningococci is not essential for cytokine responses, which are, therefore, to a considerable extent independent of SR-A. Our in vitro data provide a firm foundation for further investigation of M in vivo and suggest that M take up N. meningitidis directly via SR-A and may be critical in the innate host immune response to meningococci.
SR-A was the predominant receptor on BMM for nonopsonic uptake of meningococci. It was shown previously that the contribution of SR-A to uptake of bacteria varied depending on the strain (25). This suggested that some bacteria might use SR-A almost exclusively while others would be entirely SR-A independent, e.g., various strains of Haemophilus influenzae (L. Peiser and S. Gordon, unpublished data). As our culture system, which includes L-cell conditioned medium containing M colony-stimulating factor, results in up-regulated SR-A expression, it is likely that in other M populations SR-A is not an exclusive receptor for the organism. Indeed, uncharacterized SR in human M contributed approximately 60% of uptake. Other candidate receptors for uptake include molecules reported previously to bind N. meningitidis that are also expressed on M, such as CD46 and CD66 (19, 43). MARCO and CD36 are other candidate SR; however, CD36 does not bind bacteria (24), and MARCO, which binds E. coli and Staphylococcus aureus (4), is normally expressed on only a small subset of M.
The microbial ligand for direct binding of N. meningitidis to SR-A is presently unknown but appears to be remarkably specific for SR-A. Furthermore, the particular requirement for SR-A was conserved over the whole genus Neisseria. Candidate ligands included the negatively charged capsule, pili, LPS, and other outer membrane proteins, but we have found that the presence or absence of capsule, Opa, Opc, or pili did not affect SR-A-dependent uptake. Soluble lipid A moiety has been implicated previously as an SR-A ligand (11) and was the best candidate molecule for SR-A recognition. Lipid A is the active component of endotoxin, and binding to M results in activation and endotoxic shock (29). In intact bacteria, the availability for SR-A binding is unclear, as the buried lipid A may be sterically hindered. However, N. meningitidis has a large number of membrane blebs and outer membrane vesicles that contain outer membrane and lipid A (27), which may also allow access to SR-A for binding. The lpxA mutant of 44/76 engineered by Steeghs and colleagues completely lacks LPS (33), and it was intriguing to find that SR-A recognition of this mutant was unaltered. Preliminary studies of the lpxA mutant surface composition have shown that most major membrane molecules are present but there is increased expression of an Opa protein (33). While further studies are needed to define the actual ligand for SR-A, we can conclude that it is not LPS or a molecule that requires the presence of LPS in the membrane for appropriate expression.
In these studies, the M secretory response to N. meningitidis was uncoupled from cellular ingestion. Survival assays and EM showed that WT M killed most bacteria at early stages of ingestion, and although the level of uptake was dramatically reduced, SR-A-/- M showed a similar pattern of bacterial clearance. The M used here resembled resident, immunologically nonactivated M (8), and although the mode of killing in this system is still unknown, intraphagosomal destruction of N. meningitidis was observed by EM. Similar results were obtained in the presence or absence (not shown) of gentamicin, confirming that the clearance of N. meningitidis was not due to slow killing by the antibiotic, as evidence exists that aminoglycoside antibiotics may not be totally excluded from entering phagocytic cells (22). M possess a number of intra- and extracellular mechanisms capable of destroying pathogens, including the respiratory burst, acid hydrolases, and lysozyme, and further study is required to test their involvement in M-mediated killing of meningococci.
High levels of proinflammatory cytokines can be correlated with meningococcal-disease severity (1, 46), and following contact with N. meningitidis, WT and SR-A-/- cells rapidly produced cytokines. Again, these responses were largely SR-A independent. Intracellular-cytokine assays showed that most WT and SR-A-/- M produced TNF- irrespective of uptake. SR-A-/- M generally secreted slightly less cytokine, but the difference was variable even over a range of MC58 doses and, in WT M, was not blocked by 2F8 (not shown). Transient surface contacts or shedding of the LPS from the bacterial surface may be responsible for this activation of M signaling. Soluble products in the bacterial preparation were able to elicit cytokines to some extent, and few bacteria were observed within SR-A-/- M, although some extracellular organisms were observed close to the plasma membrane.
We demonstrated that M required the presence of TLR-4 to mediate cytokine secretion. C3H/HeJ M were unable to generate high levels of cytokine following N. meningitidis challenge compared with C3H/HeN controls, but ingestion via the receptor was unaffected. Also, although LPS was not the meningococcal ligand for SR-A, it was required for the induction of proinflammatory cytokines by M. N. meningitidis bacteria lacking LPS were unable to activate WT and SR-A-/- cells, even though ingestion via SR-A was normal. Some TLR-4- and lipid A-independent induction of cytokines was observed, but in most cases it was minor. Interestingly, IL-6 production appeared to have the greatest lipid A-independent component. Previous studies with N. meningitidis lpxA mutants showed that these organisms stimulated proinflammatory cytokine release via TLR-2 (28). The membrane molecules responsible for TLR-4-independent activation have not been identified (17), but candidate molecules include the outer membrane proteins, Opa/Opc and porin, peptidoglycan, and other lipoproteins.
The hallmark of PRM molecules is the ability to recognize conserved motifs on pathogen cell surfaces directly. Although, it is difficult to determine what role these nonopsonic receptors would play in vivo when competing with the opsonic receptors and serum opsonins, animal knockout studies have clearly shown that these receptors, specifically SR-A and TLR-4, are important in other bacterial infections. The significance of our findings remains to be tested in vivo to determine if SR-A is specifically targeted by N. meningitidis to assist in the initial stages of infection before the bacteria become opsonized by complement or antibodies or if this is a M clearance mechanism. Monocytes (as well as neutrophils) lack SR-A, but this receptor is present on most mature tissue M populations (16). Resident M are widely distributed throughout the body, including the nasopharynx, but are also present in the choroid plexus, the liver, and the adrenal gland, where they are in direct contact with CSF and blood (9). They are therefore well placed for clearance and localization of circulating organisms and LPS via SR-A. The survival of bacteria following uptake by resident M may facilitate their dissemination. Once M become primed by immune activation, SR-A may protect the host from LPS-induced septic shock, mediated by SR-A-independent M receptors (12). However, the pathogenesis of meningococcal infection cannot be readily studied in murine models, as N. meningitidis is not a natural pathogen of mice and is quickly cleared when mice are infected without the presence of large doses of iron. We showed that human SR-A transfected in CHO cells was able to bind N. meningitidis, and thus examination of the relative roles of SR-A, other SR, and non-SR in human subjects may provide clues to the variation in susceptibility to the disease and its serious complications.
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ACKNOWLEDGMENTS |
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L.P. is supported by a Goodger Scholarship, and work in the laboratories of S.G. and E.R.M. is supported by grants from the Medical Research Council, United Kingdom, and the Wellcome Trust. Facilities for microscopy were provided by a grant from the Wellcome Trust.
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FOOTNOTES |
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