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Identification of New Antigens in Visceral Leishmaniasis by Expression Cloning and Immunoblotting with Sera of Kala-Azar Patients from Bihar, India
Infect. Immun. Theinert et al. 73: 7018

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Files in this Data Supplement:

  • Supplemental file 1 - Table S1. Summary of kala-azar patients from Bihar, India, and their seronegativity. Table S2. Primers used for cloning identified antigens. Fig. S1. Serological reactivity to L. donovani promastigotes in sera of VL patients and healthy controls. Fig. S2. Identification and purification of serologically detected LD [lowercase Greek lambda] phage expression clones. Fig. S3. PCR validation of the monoclonality of purified recombinant [lowercase Greek lambda] phages. Fig. S4. Expression of leishmania antigens in E. coli BL21(DE3).




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