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Infection and Immunity, January 2006, p. 769-772, Vol. 74, No. 1
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.1.769-772.2006

A {sigma}28-Regulated Nonflagella Gene Contributes to Virulence of Campylobacter jejuni 81-176

Scarlett Goon,1 Cheryl P. Ewing,1 Maria Lorenzo,2 Dawn Pattarini,1 Gary Majam,1 and Patricia Guerry1*

Enteric Diseases Department, Naval Medial Research Center, Silver Spring, Maryland 20910,1 Food and Drug Administration MOD1, Beltsville, Maryland2

Received 9 August 2005/ Returned for modification 19 September 2005/ Accepted 25 October 2005


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ABSTRACT
 
A Campylobacter jejuni 81-176 mutant in Cj0977 was fully motile but reduced >3 logs compared to the parent in invasion of intestinal epithelial cells in vitro. The mutant was also attenuated in a ferret diarrheal disease model. Expression of Cj0977 protein was dependent on a minimal flagella structure.


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TEXT
 
The motility imparted by the single polar flagella of Campylobacter jejuni is critical for intestinal colonization and for invasion into intestinal epithelial cells (IEC) in vitro (2, 4, 10, 22, 24, 26, 32, 34, 36). C. jejuni strains appear to use the flagella structure as a type III secretory organelle in the absence of specialized secretion systems for virulence factors. Thus, C. jejuni secretes a set of so-called Cia proteins upon coculture with IECs (19, 20, 29). Mutation of ciaB resulted in loss of secretion of all Cia proteins and reduced invasion of IECs (19, 20, 29). Although the Cia proteins are synthesized in nonmotile mutants, secretion required a minimum flagella filament structure (20). A second protein, FlaC, which shows sequence similarity to flagellin but which is not required for motility or flagella biogenesis, is also secreted through the flagella apparatus (31). FlaC, which is expressed and secreted independently of CiaB, binds to HEp2 cells in vitro and modulates invasion (31).

A recent microarray study by Carillo et al. (5) reported up-regulation of several {sigma}28 and {sigma}54 nonflagella genes in a more virulent variant of the genome strain NCTC 11168 (28). These same genes were also down-regulated in two flagella mutants of NCTC 11168. We have confirmed that many of these same genes are also down-regulated in flagella mutants in C. jejuni 81-176 (S. Goon, C. P. Ewing, and P. Guerry, unpublished data), and here we further characterize one of these {sigma}28-regulated genes, Cj0977.

The Cj0977 proteins (Mr, 21.2 kDa; pI 4.8) from 81-176 and NCTC 11168 were 98% identical. The Cj0977 protein lacks a leader sequence or transmembrane domains and is predicted to be cytoplasmic. The protein is conserved within the Proteobacteria and shares some similarity with initiation factor eIF-2B (GenBank accession no. NP_214448). A Cj0977::cat mutant in C. jejuni 81-176 was fully motile (Fig. 1A) and produced a normal flagella filament (Fig. 1B). Purified flagellin from the mutant displayed an isoelectric focusing (IEF) pattern identical to that of 81-176 (Fig. 1C), suggesting that Cj0977 is not involved in flagellin glycosylation (7, 9, 23, 33). There was no difference in growth kinetics of the mutant and the parent 81-176 in Mueller-Hinton (MH) broth, nor were there any changes in either capsular polysaccharide by immunoblot or lipooligosaccharide mobility on silver-stained gels (data not shown).



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  		FIG. 1. Characterization of the Cj0977 mutant. A. Motility agar plates. Wild-type 81-176 and the Cj0977 mutant were inoculated into 0.4% MH agar plates and grown for 48 h at 37°C. B. Electron micrographs of the wild type and the Cj0977 mutant. C. Isoelectric focusing gel of purified flagellins (7). Flagellin from a pseA mutant that lacks an acetamidino form of pseudaminic acid (33) is shown for comparison. The position of pI standards is indicated on the left.

The Cj0977 mutant and a complement in which the wild-type allele linked to a Kmr marker was inserted into the astA gene of the mutant (37) were compared to 81-176 for their ability to invade INT407 cells. The mutant invaded at <0.0015% of the input inoculum (>1,000-fold reduced compared to the wild type), as shown in Fig. 2A, and invasion was restored in the complement. For comparison, mutations were constructed in 81-176 in ciaB and flaC. As seen in Fig. 2B, both mutants were reduced in invasion compared to 81-176 but much less so than the Cj0977 mutant and less than the corresponding mutations in other strains (19, 20, 29, 31).



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  		FIG. 2. Invasion of C. jejuni strains into INT407 cells. The data are presented as the percentage of the input inoculum that survived gentamicin killing (1, 27, 36). A. Invasion of 81-176, the Cj0977 mutant, and the complement (Cj0977-C). The complement was constructed as follows. A wild-type copy of Cj0977 with its promoter was PCR amplified with primers that introduced HindIII and EcoRI sites at either end, and the amplicon was cloned into pBluescript. After sequence analysis to confirm that no mutations had been introduced by PCR, a kanamycin resistance cassette from pILL600 (21) was cloned into the BamHI site in the multiple cloning site to generate pSG977-Km7. Plasmid pSG977-Km7 was digested with XhoI and XbaI to release a fragment of approximately 2.1 kb. This fragment was gel purified, blunted with Klenow, and cloned into a unique EcoRV site within the arylsulfatase (astA) gene of 81-176 as cloned into pYG660 (40), and this plasmid was used to electroporate the Cj0977 mutant to Kmr. A double crossover was confirmed by PCR. B. Invasion of 81-176 and isogenic mutants of ciaB and flaC. These mutants were constructed by transposition using an in vitro Tn5-based transposition system (Epicentre, Madison, WI) with a Campylobacter chloramphenicol resistance (cat) cassette into Escherichia coli clones of the 81-176 genes, as described previously (11, 12). The position of the inserts was determined by DNA sequence analysis prior to electroporation into 81-176. The position of the cat insert into flaC was at base pair 167 of the 759-bp open reading frame; the position of the insertion into ciaB was at base pair 1227 of the 1,833-bp open reading frame.

We examined expression of the Cj0977 protein in 10 mutants in the flagella regulon of 81-176 as shown in Table 1. All of the mutants in Table 1 were nonmotile, except for the {Delta}fliA mutant, which has a truncated flagella filament that confers reduced motility (13). Electron microscopic examination indicated that the remaining mutants were bald, except for the flaA flaB mutant and the fliD mutant, both of which produced hook structures. Representative electron micrographs are shown in Fig. 3. The Cj0977 protein could be observed in immunoblots of whole-cell preparations of wild-type 81-176 and the complement but was missing in the mutant, as seen in Fig. 4B. Figure 4 also shows that no Cj0977 protein could be detected in the fliA mutant, and Cj0977 protein levels were significantly reduced in mutants in flgR, flgS, rpoN, flgE, flhA, flhB, and fliR. The level of expression appeared to be slightly reduced in a flaA flaB double mutant, and expression appeared to be equivalent to or perhaps increased in the fliD mutant. The same preparations were also immunoblotted with anti-Omp50 (3) antibody to confirm equal loading (Fig. 4A and C).


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  		TABLE 1. Flagella mutants of C. jejuni 81-176 used in this study



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  		FIG. 3. Electron micrographs of selected flagella mutants. A. fliR; B. flgE; C. flaA flaB; D. fliD. The fliR and flgE mutants were bald, but the flaA flaB double mutant and the fliD mutant produced hook structures, visible at both poles. An image of wild-type 81-176 is shown in Fig. 1. The flaA flaB mutant was constructed as follows. A 446-bp PCR product was generated using primers within Cj1341, the gene adjacent to flaA, and the 5' end of flaA; these primers introduced SacII and BamHI sites at either end. This amplicon was cloned into pBluescript to generate plasmid pCE101. A 310-bp PCR product was generated using primers within the 3' end of flaB and the adjacent Cj1337 gene that introduced XhoI and BamHI sites, respectively, and the amplicon was cloned into pCE101. After DNA sequence analysis of the insert in the resulting plasmid (pCE102) insert, a chloramphenicol cassette from pRY109 (35) was cloned into the unique BamHI site to generate pCE103, and this plasmid was used to electroporate 81-176 to Cm resistance. All other mutants were generated using an in vitro Tn5-based transposition system, as described previously and in the legend to Fig. 2 (11, 12).



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  		FIG. 4. Coregulation of Cj0977 with the flagella regulon. Whole-cell preparations were electrophoresed on sodium dodecyl sulfate-12.5% polyacrylamide gel electrophoresis gels, transferred to nitrocellulose, and immunoblotted with anti-Omp50 antiserum (A and C) or anti-Cj0977 antiserum (B and D). Both antisera were generated in rabbits (Harlan, Madison, WI) against recombinant forms of the proteins. In panels A and B, "m" refers to the Cj0977 mutant and "c" refers to the complement. The bands in panels A and C were run with a 50-kDa protein marker, and the bands in panels B and D were between a 20- and 25-kDa protein marker. wt, wild type.

The Cj0977 mutant and the complemented strain were compared to wild-type 81-176 in the ferret diarrhea model (1). The Cj0977 mutant was attenuated compared to both the parent and the complement, as shown in Fig. 5. Thus, fewer animals developed diarrhea over the course of the experiment when fed the mutant (3 of 16 ferrets) compared to the wild type (12 out of 16; P = 0.0038) or complement (11 out of 16; P = 0.0113), and those animals that did develop diarrhea following feeding of the mutant displayed symptoms later than those fed the wild type or complement.



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  		FIG. 5. Cj0977 mutant is attenuated in the ferret diarrheal disease model. The cumulative percentages of the total animals that developed diarrhea over 4 days postinfection are shown. The total number of animals per group was 16.

Although we have not been able to demonstrate secretion of Cj0977 into the supernatant (data not shown), the invasion defect in the Cj0977 mutant of 81-176 was greater than mutation of either ciaB or flaC in the same strain. In TGH9011, the flaC mutant invaded at about 14% of the level of the wild type compared to 43% of the level of the wild type in 81-176. Similarly, while the ciaB mutant in F38011 invaded at levels that were about 50-fold lower than those of the parent, the attenuation in 81-176 was 72% of the wild type. The discrepancy may be due, in part, to technical differences in invasion assays and cell lines used among different laboratories. However, the observed variations may reflect inherent differences in microtubule and microfilament uptake mechanisms among strains (6, 16-18, 25, 27, 30).

While much additional work remains to be done to understand the mechanism by which Cj0977 contributes to virulence of 81-176 at the molecular and cellular levels, these preliminary data establish a role for this protein in pathogenesis. This is the first report of coregulation of a virulence determinant with the C. jejuni flagella regulon, and it is similar to reports of {sigma}28-regulated virulence genes in other pathogens (8, 14, 15). The data also provide an additional explanation of the role of flagella in pathogenesis of C. jejuni, namely that this virulence gene, and perhaps others that are also {sigma}28 or {sigma}54 regulated (5 and Goon et al., unpublished), form an integral part of the flagella regulon.


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ACKNOWLEDGMENTS
 
We thank Dave Hendrixson and Vic DiRita for the rpoN and fliA deletion mutants of 81-176, Lindsay Holder and Anahita Kiavand for technical assistance, and Robert Williams for electron microscopy.

This work was supported by RO1 AI043559 and NMRC work unit 6000.RAD1.DA3.A0308 from the Military Infectious Diseases Research Program.


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FOOTNOTES
 
* Corresponding author. Mailing address: Enteric Diseases Dept., Naval Medical Research Center, 503 Robert Grant Ave., Silver Spring, MD 20910. Phone: (301) 319-7662. Fax: (301) 319-7679. E-mail: guerryp{at}nmrc.navy.mil. Back

Editor: J. T. Barbieri


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Infection and Immunity, January 2006, p. 769-772, Vol. 74, No. 1
0019-9567/06/$08.00+0     doi:10.1128/IAI.74.1.769-772.2006




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