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Infection and Immunity, October 2006, p. 5955-5963, Vol. 74, No. 10
0019-9567/06/$08.00+0     doi:10.1128/IAI.00481-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Immunogenicity of Duffy Binding-Like Domains That Bind Chondroitin Sulfate A and Protection against Pregnancy-Associated Malaria

Nivedita Bir,1 Syed Shams Yazdani,1 Marion Avril,2 Corinne Layez,2 Jürg Gysin,2 and Chetan E. Chitnis1*

Malaria Group, International Centre for Genetic Engineering, and Biotechnology (ICGEB), New Delhi 110067, India,1 Unité de Parasitologie Expérimentale, Faculté de Médecine, Université de la Méditerranée, Marseille, France2

Received 24 March 2006/ Returned for modification 4 June 2006/ Accepted 3 July 2006


   ABSTRACT
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 References
 
Sequestration of Plasmodium falciparum-infected erythrocytes in the placenta is implicated in pathological outcomes of pregnancy-associated malaria (PAM). P. falciparum isolates that sequester in the placenta primarily bind chondroitin sulfate A (CSA). Following exposure to malaria during pregnancy, women in areas of endemicity develop immunity, and so multigravid women are less susceptible to PAM than primigravidae. Protective immunity to PAM is associated with the development of antibodies that recognize diverse CSA-binding, placental P. falciparum isolates. The epitopes recognized by such protective antibodies have not been identified but are likely to lie in conserved Duffy binding-like (DBL) domains, encoded by var genes, that bind CSA. Immunization of mice with the CSA-binding DBL3{gamma} domain encoded by var1CSA elicits cross-reactive antibodies that recognize diverse CSA-binding P. falciparum isolates and block their binding to placental cryosections under flow. However, CSA-binding isolates primarily express var2CSA, which does not encode any DBL{gamma} domains. Here, we demonstrate that antibodies raised against DBL3{gamma} encoded by var1CSA cross-react with one of the CSA-binding domains, DBL3X, encoded by var2CSA. This explains the paradoxical observation made here and earlier that anti-rDBL3{gamma} sera recognize CSA-binding isolates and provides evidence for the presence of conserved, cross-reactive epitopes in diverse CSA-binding DBL domains. Such cross-reactive epitopes within CSA-binding DBL domains can form the basis for a vaccine that provides protection against PAM.


   INTRODUCTION
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 References
 
Following repeated exposure to Plasmodium falciparum infections, adults in areas of endemicity develop immunity to clinical malaria (42, 46). However, women in areas of endemicity are uniquely susceptible to P. falciparum malaria during pregnancy (7, 36). Infection with P. falciparum is an important cause of maternal anemia and increases the risk of abortion, premature delivery, low birth weight, neonatal mortality, and infant anemia, especially in primigravidae (8, 28, 31, 35, 52). P. falciparum infections during pregnancy are frequently characterized by the sequestration of infected erythrocytes (IEs) in placental blood spaces (35), which can lead to inflammatory responses (54), deposition of fibrinoid material (57), and reduced blood flow to the fetus (18).

There has been considerable interest in understanding the molecular mechanisms that mediate placental sequestration of IEs and the reasons for the apparent lack of immunity to P. falciparum malaria in primigravidae residing in areas of endemicity. Multigravid women appear to be protected against the deleterious effects of P. falciparum infection during pregnancy (20, 36), suggesting that strain-transcending immunity develops rapidly following exposure to placental P. falciparum isolates. The mechanisms that mediate protective immunity against pregnancy-associated malaria (PAM) are not completely understood.

Adhesion studies have revealed that IEs derived from placentas predominantly bind chondroitin sulfate A (CSA) (1, 16, 24, 43). Binding to hyaluronic acid and normal immunoglobulins (Igs) may also play a minor role in placental sequestration (5, 6, 16, 23). In contrast, IEs derived from peripheral blood of P. falciparum-infected pregnant women or from that of nonpregnant donors, including children and adult men, commonly bind other endothelial receptors, such as CD36 (24). These findings suggest the possibility that the placenta may select for rare CSA-binding P. falciparum variants that are not commonly found in infected children or nonpregnant adults.

The cytoadherence of IEs to the host endothelium is mediated by variant surface proteins that belong to the P. falciparum erythrocyte membrane protein-1 (PfEMP-1) family (13). The P. falciparum genome contains ~60 var genes that encode diverse PfEMP-1 variants (3, 48, 49, 53). Expression of PfEMP-1 undergoes antigenic variation due to the switching of var gene expression during blood-stage growth (48). Immune adults residing in areas of endemicity acquire antibodies that recognize diverse PfEMP-1 variants and agglutinate diverse P. falciparum isolates (33). Antibodies directed against PfEMP-1 are thought to be important components of naturally acquired immunity to P. falciparum malaria (10). While sera from immune adult men and primigravid women residing in areas of endemicity recognize a wide range of peripheral P. falciparum isolates, they exhibit poor recognition of placental P. falciparum isolates (4, 25) and CSA-binding laboratory strains (41, 51). Following P. falciparum infection during pregnancy, women develop antibodies that show improved recognition of a wide range of placental isolates and CSA-binding laboratory strains (4, 25, 41, 51). The levels of antibodies recognizing placental isolates or CSA-binding laboratory strains are significantly correlated with parity (4, 25, 41, 51). This indicates the development of antibodies that recognize conserved epitopes on the IE surfaces of diverse placental and CSA-binding isolates. The identity of such conserved epitopes has not yet been defined, but they are likely to lie within PfEMP-1 variants that mediate adhesion to CSA. The PfEMP-1 variants that were initially implicated in CSA binding include var1CSA from P. falciparum FCR3CSA (9) and CS2var from P. falciparum CS2 (39, 40). Adhesion to CSA is mediated by the DBL3{gamma} domain of var1CSA (9) and CS2var (39, 40). Monoclonal antibodies raised against CHO cells expressing DBL3{gamma} of var1CSA and antisera raised against recombinant DBL3{gamma} expressed in insect cells recognize a wide range of placental P. falciparum isolates, suggesting that DBL3{gamma} contains conserved, cross-reactive epitopes shared by diverse CSA-binding placental isolates (15, 32). However, although var1CSA was initially implicated as the var gene responsible for CSA binding in P. falciparum FCR3CSA, subsequent studies demonstrated that the expression of another var gene, var2CSA, and not that of var1CSA, is upregulated in diverse CSA-binding parasite lines and placental isolates (19, 21, 30, 44, 56). The var2CSA gene implicated in CSA binding does not encode any DBL{gamma} domains. The reported reactivity of anti-rDBL3{gamma} sera with placental CSA-binding P. falciparum isolates is thus paradoxical.

Here, we have produced recombinant DBL3{gamma} (rDBL3{gamma}) of var1CSA in its functional form and examined its immunogenicity. We demonstrate that immunization with rDBL3{gamma} does elicit sera that cross-react with a wide range of placental isolates and block their binding to placental cryosections under static as well as physiologically relevant flow conditions. Importantly, we show that anti-rDBL3{gamma} sera cross-react with one of two CSA-binding DBL domains, namely, the DBL3X domain of var2CSA. This observation suggests that the CSA-binding DBL domains DBL3{gamma} and DBL3X share conserved B-cell epitopes and explains the paradoxical observations that anti-rDBL3{gamma} sera recognize CSA-binding P. falciparum isolates even though these parasites express var2CSA that does not encode any DBL{gamma} domains. Such conserved epitopes within diverse CSA-binding DBL domains may provide the basis for the development of vaccines that elicit cross-reactive antibodies against CSA-binding isolates and protect against PAM.


   MATERIALS AND METHODS
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 References
 
Production and characterization of functional rDBL3{gamma}. DNA encoding the DBL3{gamma} domain of var1CSA from P. falciparum FCR3 fused to a C-terminal six-histidine tag was amplified by PCR using primers 5' ACT TGC CCA TGG GAA AAC GAT GGA AAG AAA C 3' and 5' ACG AGT GCG GCC GCT CAG TGA TGG TGA TGG TGA TGC CTG TTC AAG TAA TCT GTT G 3' and a cloned fragment of the FCR3 var1CSA gene (kindly provided by Artur Scherf, Institut Pasteur, Paris, France) (9) as the template. The PCR product was cloned as a NcoI-NotI fragment downstream of the T7 promoter in expression vector pET28a+ (Novagen) and transformed into Escherichia coli BL21(DE3) (Novagen). Expression of rDBL3{gamma} with a C-terminal six-histidine tag was induced by the addition of 1 mM isopropyl-1-thio-ß-D-galactopyranoside (IPTG) to cultures of E. coli BL21(DE3)(pET28a+DBL3{gamma}) grown to mid-logarithmic phase. Induced cultures were harvested after 4 h of growth at 37°C and lysed by sonication. Inclusion bodies were collected by centrifugation and solubilized in 6 M guanidine-hydrochloride. His-tagged rDBL3{gamma} was purified under denaturing conditions by metal affinity chromatography using a Ni-nitrilotriacetic acid column (QIAGEN), refolded by the method of rapid dilution, and purified further to homogeneity by ion-exchange chromatography using SP-Sepharose (Pharmacia) and gel filtration chromatography using Superdex 75 as described earlier for other DBL domains (37, 47).

Refolded and purified DBL3{gamma} was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) before and after reduction with 5 mM dithiothreitol and detected by silver staining. The homogeneity of rDBL3{gamma} was analyzed by reverse-phase chromatography on a C8 column as previously described (37, 47). The presence of free thiols was detected by Ellman's method with 5',5'-dithiobis(2-nitrobenzoic acid) (DTNB) (22, 37, 47).

Assessment of binding of rDBL3{gamma} to biotinylated CSA immobilized on streptavidin-coated wells by ELISA. CSA, CSB, and CSC (Calbiochem) were biotinylated using a biotinylation kit (Pierce) as described by the manufacturer. Biotinylated CSA (Bio-CSA), Bio-CSB, and Bio-CSC (100 µl of 5 µg ml–1) were immobilized in streptavidin-coated wells (Streptawell-96; Roche). Residual free sites were saturated with 3% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) with 0.05% Tween 20 (PBST) for 1 h at 37°C. rDBL3{gamma} (5 µg ml–1) was added to wells and allowed to bind overnight at 4°C. Unbound rDBL3{gamma} was removed by washing with PBST four times. A mouse monoclonal antibody against penta-histidine (penta-His) (QIAGEN) was added to each well at a 1:5,000 dilution and incubated at 37°C for 1 h to detect bound rDBL3{gamma}. Bound mouse anti-penta-His monoclonal antibodies were detected with goat anti-mouse IgG peroxidase conjugate (Sigma) (1: 5,000 dilution) and O-phenylenediamine (OPD) (Sigma). The optical density at 490 nm was measured using an enzyme-linked immunosorbent assay (ELISA) plate reader (Molecular Devices) to estimate relative binding efficiencies. Biotinylated hyaluronic acid (Bio-HA) and the recombinant receptor for complement component C1q, also known as hyaluronic acid-binding protein 1 (gC1qR/HABP1) (17) (kindly provided by Anup Biswas and Kasturi Datta, Jawaharlal Nehru University, New Delhi, India) were used as controls.

Immunization of BALB/c mice. BALB/c mice were immunized intraperitoneally with 25 µg of rDBL3{gamma} formulated in 250 µl Freund's complete adjuvant and subsequently given three booster immunizations with 25 µg of rDBL3{gamma} formulated with Freund's incomplete adjuvant on days 21, 42, and 73. Sera were collected on days 14, 35, 56, 70, and 87 and stored at –80°C until use.

Inhibition of binding of CSA to rDBL3{gamma} by anti-rDBL3{gamma} sera raised in mice. rDBL3{gamma} (100 µl of 5 µg ml–1 per well) was coated in 96-well polystyrene microtiter plates (Nunc) by incubation overnight at 4°C followed by blocking with 3% BSA. Microtiter plate wells were incubated for 1 h at 37°C with different dilutions of mouse sera raised against rDBL3{gamma} or human sera from areas of endemicity. Pooled preimmune mice sera were used as controls. One hundred microliters of Bio-CSA (5 µg ml–1) was added to each well and allowed to bind for 1 h at 37°C. Following four washes with PBST, bound Bio-CSA was detected with Extravidin peroxidase and OPD (Sigma). The optical densities at 490 nm were used to estimate the relative binding efficiencies to CSA in the presence of test sera compared to those of controls.

Recognition of P. falciparum IEs by anti-rDBL3{gamma} mouse sera using FACS. Recognition of P. falciparum IEs by mouse anti-rDBL3{gamma} sera was evaluated by fluorescence-assisted cell sorting (FACS) as described earlier (50). Briefly, 5 x 106 erythrocytes from asynchronous P. falciparum cultures were washed twice with RPMI medium, pH 6.8, and incubated for 30 min at 37°C with ethidium bromide (5 µg ml–1). After two washes, cells were incubated with anti-rDBL3{gamma} mouse sera diluted 1:20 for 30 min at 4°C. Preimmune mouse serum was used as a negative control. Cells were washed two times with RPMI medium, pH 6.8, and incubated with anti-mouse IgG (heavy plus light chains) chicken at a dilution of 1:200 for 30 min on ice. Erythrocytes were washed twice with RPMI medium and incubated with a goat anti-chicken IgY conjugated to Alexa-488 (Molecular Probes) at a dilution of 1:200 for 30 min on ice. The recognition of intact IEs by antibodies was quantified with a Coulter EPICS flow cytometer and expressed as the mean Alexafluor intensity of ethidium bromide-gated IEs. Mouse sera raised against rDBL3{gamma} were tested at a 1:20 dilution by both FACS and liquid immunofluorescence assay (L-IFA). Preimmune mouse sera were used as controls.

Inhibition of cytoadhesion of IE under static conditions by anti-rDBL3{gamma} sera. Static cytoadherence assays were performed as described previously (26). Briefly, Saimiri monkey brain endothelial C2 (SBEC C2) cells were grown to confluence on 12-well slides (Bio-Rad). Trophozoite- and schizont-stage IEs (20 µl of 107 IEs ml–1) were added to each well to allow binding. Heat-inactivated (56°C) anti-rDBL3{gamma} mouse sera (dilution of 1:20) and soluble CSA (100 µg ml–1) (Calbiochem) were tested for inhibition of binding. Cytoadherence medium and mouse preimmune serum served as controls. In another experiment, SBEC C2 cells were preincubated with rDBL3{gamma} (200 µg ml–1) prior to the addition of IEs. Adherent IEs were detected by Giemsa staining and scored by light microscopy. Results were expressed as percent inhibition of binding with respect to the appropriate controls.

Inhibition of cytoadherence of IEs to placental cryosections with anti-rDBL3{gamma} mouse sera under flow conditions. The cytoadherence of IEs on placental cryosections was tested under flow conditions as described earlier (2, 26). Briefly, slides of four cryosections from each of two different placentas were mounted in a cell adhesion flow chamber (CAF-10; Immunetics) held in place by vacuum. The system was connected to an infusion/withdrawal pump (model 210P; KD Scientific) to control the flow of IE suspension or cell-free medium through the perfusion chamber. Reservoirs containing the IE suspension or cytoadhesion medium (RPMI medium, pH 6.8) were connected to the outlet of the perfusion chamber through a three-way valve. The cryosections were perfused with IEs at 5% parasitemia (mature stages) and 25% hematocrit in RPMI medium for 10 min at a shear stress of 0.05 Pa. The chamber was then flushed with RPMI medium to remove nonadherent IEs. Adherent IEs were observed with an inverted microscope (Eclipse TE 200; Nikon) with a PlanFluor 40/0.60 objective (Nikon). Bound IEs were counted on 10 randomly distributed fields with an area of 0.081 mm2. Assays were performed in the presence of anti-rDBL3{gamma} mouse serum (1:20 dilution) to test the ability of the IEs to block adhesion. Results were expressed as percent inhibition of binding with respect to binding in presence of preimmune sera.

Parasites and cells. P. falciparum laboratory strains as well as field isolates were cultured in O+ erythrocytes in RPMI 1640 (Sigma, France) in candle jars as described previously (55). P. falciparum laboratory strains FCR3CSA (9) and BC-1-CSA (kindly provided by Artur Scherf, Institut Pasteur, Paris, France) and P. falciparum placental isolates 24-CSA, 42-CSA, 42DJ-CSA, 193-CSA, 938-CSA, and 939-CSA (29), all of which bind CSA, were used for the study. In addition, P. falciparum strains FCR3CD36 (9), D10, and T996 (kindly provided by David Walliker, University of Edinburgh) and peripheral isolates RAJ-68, RAJ-104, and JDP8 (collected from regions of India where malaria is endemic and kindly provided by C. R. Pillai, Malaria Research Centre, Delhi, India), which do not bind CSA, were used as controls. The CSA-binding ability of placental isolates and CSA-binding laboratory strains was maintained by periodic panning on Sc17 cells (29).

Placental cryosections. Four cryosections from each of two different normal placentas collected in France were mounted sideways in the centers of 76- by 25-mm superfrost glass slides (Menzel-Glaser, Germany) for cytoadherence analysis under flow conditions. Placental cryosections (7 µm) were cut with a cryotome (CM 3050; Leica, Germany), air dried, fixed in 2.5% paraformaldehyde in PBS, pH 7.2, for 30 min, washed with PBS, dried, and preserved in an airtight box at –80°C until use. Before being used in adhesion assays, the slides were air dried quickly to prevent the condensation of water. Informed consent was obtained from all the participants. Biological material was sampled in strict accordance with the Mattei Law 666-8.

Identification of CSA-binding DBL domains of var2CSA by expression on the surface of mammalian cells. Plasmids were constructed to express the DBL domains (DBL1X to DBL6{varepsilon}) of var2CSA of P. falciparum 3D7 (PFL0030c) on the surface of mammalian cells as described previously for other DBL domains (12, 34, 38). Amino acid boundaries of each DBL domain construct were as follows: for DBL1X, amino acids (aa) 46 to 343; for DBL2X, aa 535 to 934; for DBL3X, aa 1214 to 1562; for DBL4{varepsilon}, aa 1581 to 1931; for DBL5{varepsilon}, aa 1983 to 2291; and for DBL6{varepsilon}, aa 2333 to 2617. Briefly, the DBL domains were fused to the signal sequence and transmembrane domain of herpes simplex virus glycoprotein D (HSV gD) to allow targeting to the mammalian cell surface as described previously (12, 34, 38). The plasmid pRE4 (kindly provided by Roselyn Eisenberg and Gary Cohen), which contains the gene for HSV gD (14), was digested with PvuII and ApaI to excise the central region encoding amino acids 33 to 248 of HSV gD. DNA fragments encoding DBL domains of 3D7 var2CSA were amplified by PCR using Pyrococcus furiosus DNA polymerase (Stratagene) by use of var2CSA-specific primers and cloned into PvuII and ApaI sites in the vector pRE4 as previously described (12) to yield plasmids pRE4-DBL1X, pRE4-DBL2X, pRE4-DBL3X, pRE4-DBL4{varepsilon}, pRE4-DBL5{varepsilon}, and pRE4-DB6{varepsilon}. The DNA sequence of the insert in each expression plasmid was confirmed by sequencing with an ABI310 automated DNA sequencer. The sequence of each insert was identical to that reported for 3D7 var2CSA (PFL0030c; GenBank accession no. NP_701371). Mammalian 293T cells were transfected as described below to express the DBL domains (DBL1X to DBL6{varepsilon}) on the surface. Transfected cells were tested for binding to CSA as described previously (34). CSA-binding assays were performed in the presence of anti-rDBL3{gamma} mouse sera and preimmune sera to test the abilities of sera to block binding.

Mammalian cell culture, transfection, and immunofluorescence assays. 293T cells were cultured in Dulbecco's modified Eagle's medium (Invitrogen) with 10% heat-inactivated fetal calf serum in a humidified CO2 (5%) incubator at 37°C. Fresh monolayers of 40 to 60% confluent 293T cells growing in 35-mm-diameter wells were transfected with 2 µg of plasmid DNA using Lipofectamine Plus reagent (Invitrogen) as indicated by the manufacturer. Transfected cells were used for immunofluorescence and binding assays 36 to 40 h after transfection (34). Immunofluorescence assays using mouse monoclonal antibody DL6 (kindly provided by Roselyn Eisenberg and Gary Cohen), which reacts with amino acids 272 to 279 (14) of HSV gD, or anti-rDBL3{gamma} mouse sera were performed as described earlier (12) to detect the expression of the fusion proteins. The binding of Bio-CSA to transfected 293T cells was tested as previously described (34).

Binding of refolded and purified rDBL3{gamma} to biotinylated CSA immobilized on streptavidin-coated microwells by ELISA. One hundred microliters of Bio-CSA, Bio-CSB, or Bio-CSC was immobilized on each well of streptavidin-coated microwell plate (Stretawell-96; Roche Applied Science) as indicated by the manufacturer. Five hundred nanograms of refolded and purified rDBL3{gamma} was incubated overnight at 4°C, and unbound rDBL3{gamma} was removed by washing four times with PBST. Residual free sites in the wells were saturated with 3% BSA in PBST for 1 h at 37°C, and the wells were washed four times with PBST. One hundred microliters of anti-penta-His antibody (Sigma) diluted to 1:5,000 was added to each well, and the wells were incubated at 37°C for 1 h, followed by four washes with PBST. One hundred microliters of Extravidin peroxidase (Sigma) (1:5,000 dilution) was incubated in each well for 1 h at 37°C. Bound immunocomplexes were detected with OPD (Sigma). Absorbance was measured at 490 nm using an ELISA reader (Molecular Devices).

Inhibition of binding of rDBL3{gamma}-CSA binding by anti-rDBL3{gamma} sera raised in mice. Anti-rDBL3{gamma} mouse sera were tested for inhibition of the rDBL3{gamma}-CSA interaction. Microtiter plate wells were coated with 100 µl of rDBL3{gamma} (5 µg ml–1). Plates were incubated at 4°C overnight and blocked with 3% BSA as described above. Microtiter plate wells were incubated with different dilutions of mouse antibodies raised against rDBL3{gamma}. Preimmune sera were used as controls. Five hundred nanograms of Bio-CSA was incubated in each well at 37°C for 1 h. After being washed with PBST four times, the well was incubated with Extravidin peroxidase (Sigma) for 1 h at 37°C. Results were expressed as the percentages of binding observed with respect to the values for controls.


   RESULTS
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 References
 
Purity, homogeneity, and functional activity of rDBL3{gamma}. The DBL3{gamma} domain of var1CSA derived from P. falciparum FCR3 was expressed in E. coli, purified from inclusion bodies under denaturing conditions by metal affinity chromatography, refolded by rapid dilution, and purified to homogeneity by ion-exchange and gel filtration chromatography. Refolded and purified rDBL3{gamma} was analyzed for purity, homogeneity, and functional activity. A single band of the expected size (~38 kDa) was detected on silver-stained SDS-PAGE gels (Fig. 1). rDBL3{gamma} migrated slower on SDS-PAGE gels after reduction with dithiothreitol, indicating that the refolded protein contained disulfide linkages (Fig. 1). The free thiol content in rDBL3{gamma}, which contains 10 cysteines, was measured using Ellman's method (22). The detection limit for free thiols by this assay was 30 µM. No free thiols were detected in rDBL3{gamma} at a protein concentration of 50 µM, indicating that >94% of cysteines were disulfide linked. The homogeneity of refolded rDBL3{gamma} was analyzed by reverse-phase chromatography. rDBL3{gamma} eluted from a C8 column as a single symmetric peak, suggesting that refolded rDBL3{gamma} was conformationally homogeneous (Fig. 1).


Figure 1
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  		FIG. 1. Characterization of refolded and purified DBL3{gamma} domain (rDBL3{gamma}) of FCR3 var1CSA. (A) Mobility of rDBL{gamma} as determined by SDS-PAGE. Refolded rDBL{gamma} has lower mobility in SDS-PAGE after reduction with dithiothreitol (+DTT), indicating the presence of disulfide linkages. Molecular mass markers (M) in kDa are shown. (B) Reverse-phase chromatography profile of rDBL3{gamma}. Refolded DBL3{gamma} elutes as a single, symmetric peak upon reverse-phase chromatography on a C8 column, indicating that it is conformationally homogenous. AU, absorbance units (280 nm).

 
The functional activity of rDBL3{gamma} was examined by testing the binding of rDBL3{gamma} with its receptor, CSA. Biotinylated receptors, namely, Bio-CSA, Bio-CSB, Bio-CSC, and Bio-HA, were immobilized on streptavidin-coated ELISA plate wells and incubated with refolded rDBL3{gamma} to allow binding. Bound rDBL3{gamma} was detected using a mouse monoclonal antibody against the C-terminal penta-histidine tag. Binding of rDBL3{gamma} was observed only for wells coated with Bio-CSA (Fig. 2). The lack of binding to CSB, CSC, and HA indicates that rDBL3{gamma} bound CSA with specificity. The human endothelial receptor gC1qR/HABP1, which binds the globular head of complement component C1q as well as HA (17), and the receptor-binding domain, region II (PvRII), of P. vivax Duffy binding protein (12), were used as control ligands. PvRII did not bind CSA, CSB, CSC, or HA (Fig. 2). Recombinant gC1qR/HABP1 bound HA as expected but not CSA, CSB, or CSC (Fig. 2).


Figure 2
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  		FIG. 2. Binding of rDBL3{gamma} to CSA. Binding of rDBL3{gamma} to Bio-CSA, Bio-CSB, Bio-CSC, and Bio-HA immobilized on streptavidin-coated microwells was detected using a mouse monoclonal antibody against penta-His. Recombinant PvRII, the binding domain of PvDBP, and recombinant gC1qR/HABP1, which binds HA, were used as control ligands. rDBL3{gamma} binds CSA but not CSB, CSC, or HA. gC1qR/HABP1 binds HA but not CSA, CSB, or CSC. PvRII does not bind any of the receptors tested. OD, optical density.

 
Mouse sera raised against rDBL3{gamma} recognize diverse CSA-binding P. falciparum laboratory strains and placental field isolates. Pooled sera from five mice immunized with rDBL3{gamma} detected rDBL3{gamma} by ELISA up to a dilution of 1:100,000 and blocked the binding of rDBL3{gamma} to CSA in an ELISA-based binding assay with 50% inhibition at a dilution of 1:500. Anti-rDBL3{gamma} mouse sera were tested for recognition of diverse P. falciparum laboratory strains and field isolates by FACS and L-IFA. The P. falciparum laboratory strains and field isolates were first tested for binding to SBEC C2 cells, which display CSA on their surface (26). P. falciparum placental isolates bound SBEC C2 cells (Table 1) . The addition of CSA (100 µg ml–1) inhibited the binding of placental isolates and CSA-binding laboratory strains to SBEC C2 cells, confirming that these parasites used CSA as a receptor for adhesion to SBEC C2 cells (Table 1). Preincubation of SBEC C2 cells with rDBL3{gamma} also inhibited the adhesion of IEs (Table 1). Anti-rDBL3{gamma} mouse sera recognized CSA-binding P. falciparum laboratory strains and placental isolates by FACS as well as L-IFA (Fig. 3 and 4; Table 1). In contrast, anti-rDBL3{gamma} mouse sera did not recognize P. falciparum laboratory strains or field isolates that did not bind CSA (Fig. 3; Table 1). In the FACS studies, specific shifts characteristic of Alexafluor-stained IEs were observed in the cases of CSA-binding laboratory strains, such as FCR3CSA, and placental isolates, such as 193-CSA (Fig. 3). The fraction of erythrocytes with specific shifts was higher for CSA-binding isolates than for laboratory isolates such as FCR3CD36 and T996 and peripheral isolates RAJ68, RAJ104, and JDP8, which did not bind CSA (Fig. 3; Table 1). The pattern of recognition of CSA-binding IEs by anti-rDBL3{gamma} sera by L-IFA clearly indicated reactivity with the surface of IEs (Fig. 4). Anti-rBL3{gamma} sera thus recognized common conserved epitopes that are shared by diverse CSA-binding P. falciparum isolates. Preimmune sera collected from mice prior to immunization with rDBL3{gamma} showed no reactivity with any of the parasites when tested by FACS or L-IFA. While reactivity with FCR3CSA was observed by FACS when anti-mouse IgG goat serum coupled to fluorescein isothiocyanate was used for secondary staining with anti-rBL3{gamma} mouse sera for primary staining, no reactivity was observed when fluorescein isothiocyanate-coupled anti-mouse IgM goat serum was used for secondary staining (data not shown). This rules out the possibility that the reactivity of anti-rBL3{gamma} mouse sera with CSA-binding isolates was due to nonspecific binding of mouse IgM.


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  		TABLE 1. Recognition of P. falciparum IEs by anti-DBL3{gamma} mouse sera by L-IFA and FACS and inhibition of adhesion of P. falciparum IEs to SBEC C2 cells by CSA and recombinant DBL3{gamma}

 

Figure 3
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  		FIG. 3. Recognition of diverse P. falciparum isolates by mouse sera raised against rDBL3{gamma} of FCR3 var1CSA by use of flow cytometry. Mouse sera raised against rDBL3{gamma} were tested for recognition of P. falciparum FCR3CD36 (A) and T996 (B), which do not bind CSA, and of FCR3CSA (C) and 193 (D), which bind CSA. Gray areas represent signals from preimmune sera.

 

Figure 4
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  		FIG. 4. Recognition of surfaces of P. falciparum IEs by anti-rDBL3{gamma} mouse sera using L-IFA. Mouse sera raised against rDBL3{gamma} of FCR3 var1CSA react with the IE surface of P. falciparum laboratory strain FCR3CSA and that of placental isolate 193CSA, which bind CSA, but not with that of P. falciparum laboratory strain FCR3CD36 or that of peripheral field isolate JDP8, which do not bind CSA. Parasites were stained with DNA-intercalating dye DAPI (blue) to identify IEs and with anti-rDBL3{gamma} mouse sera followed by Alexafluor 488-conjugated chicken anti-mouse IgG (green).

 
Mouse sera raised against rDBL3{gamma} block adhesion of diverse CSA-binding P. falciparum laboratory strains and field isolates to Saimiri monkey brain endothelial cells and placental cryosections. Anti-rDBL3{gamma} mouse sera and preimmune mouse sera were tested for inhibition of binding of IEs to SBEC C2 cells and placental cryosections. Anti-rDBL3{gamma} sera inhibited the binding of diverse CSA-binding P. falciparum laboratory strains and placental isolates to SBEC C2 cells under static conditions (Table 2). Anti-rDBL3{gamma} sera also blocked the adhesion of diverse CSA-binding parasite isolates to placental cryosections under flow conditions (Table 2). rDBL3{gamma} was thus immunogenic and elicited cross-reactive antibodies that blocked the cytoadherence of diverse CSA-binding P. falciparum laboratory strains and placental isolates under both static and flow conditions.


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  		TABLE 2. Inhibition of adhesion of P. falciparum isolates to SBEC C2 cells under static conditions and to placental cryosections under flow conditions by anti-rDBL3{gamma} mouse sera

 
Anti-rDBL3{gamma} mouse sera recognize DBL3X of var2CSA and block its binding to CSA. Initial studies indicated that var1CSA is responsible for the CSA-binding phenotype of FCR3CSA (9). However, subsequent studies demonstrated that transcription of another var gene, var2CSA, is upregulated in CSA-binding parasites (42, 56). Here, we have expressed the DBL domains of var2CSA from P. falciparum 3D7 (DBL1X, DBL2X, DBL3X, DBL4{varepsilon}, DBL5{varepsilon}, and DBL6{varepsilon}) on the surface of mammalian 293T cells and tested them for binding to Bio-CSA. In control experiments, the DBL domains were tested for binding to Bio-CSB and Bio-CSC. The DBL3{gamma} domain of var1CSA was used as a positive control in the binding studies. The DBL2X and DBL3X domains of var2CSA specifically bound Bio-CSA in this study (Table 3). Soluble CSA, but not CSB or CSC, inhibited the binding of DBL2X, DBL3X, and DBL3{gamma} to Bio-CSA, confirming that these domains bound CSA with specificity (Table 4) . Mouse sera raised against DBL3{gamma} of var1CSA recognized DBL3{gamma} as well as DBL3X expressed on the surface of 293T cells (Table 3). Importantly, anti-rDBL3{gamma} mouse sera completely blocked the binding of both DBL3{gamma} and DBL3X domains with CSA at a dilution of 1:20 (Table 4). The DBL3{gamma} domain of var1CSA thus shares cross-reactive epitopes with the DBL3X domain of var2CSA. Antisera elicited against DBL3{gamma} did not recognize DBL2X of var2CSA and did not block its binding to CSA (Tables 3 and 4). Anti-rBL3{gamma} sera also did not recognize any of the other DBL domains of var2CSA (Table 3).


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  		TABLE 3. Binding of DBL domains of 3D7 var2CSA to CSA and recognition by mouse sera raised against DBL3{gamma} of FCR3 var1CSA

 

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  		TABLE 4. Inhibition of binding of CSA to DBL3{gamma} domain of FCR3 var1CSA and DBLX domains of 3D7 var2CSA with mouse sera raised against rDBL3{gamma}

 

   DISCUSSION
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
References
 
The epidemiology of naturally acquired immunity against placental malaria suggests that multigravid women who have experienced placental malaria rapidly develop antibodies that cross-react with a wide range of placental isolates. This suggests that placental isolates may use a limited number of CSA-binding domains that share common B-cell epitopes. The first parasite-derived domain shown to bind CSA was the DBL3{gamma} domain of var1CSA from the CSA-binding laboratory strain FCR3CSA (9). Later studies indicated that the expression of var1CSA is not restricted to placental isolates and that the expression of another gene, var2CSA, is upregulated in FCR3CSA and other CSA-binding placental and laboratory isolates (44, 45, 56). However, antibodies raised against rDBL3{gamma} of var1CSA were shown to cross-react with a wide range of CSA-binding placental and laboratory isolates (15, 32). This result was paradoxical, given that CSA-binding parasites primarily express var2CSA, which does not contain any DBL{gamma} domains. Here, we have produced recombinant DBL3{gamma} from FCR3 var1CSA in its functional form and reexamined its immunogenicity. Characterization of rDBL3{gamma} confirmed that it was pure, homogenous, and functional in that it bound CSA with specificity. We demonstrate here that the immunization of mice with rDBL3{gamma} of FCR3 var1CSA elicited antibodies that recognize the homologous parasite FCR3CSA as well as a wide range of heterologous P. falciparum laboratory strains and placental field isolates that bind CSA (Fig. 3 and 4; Table 1). In contrast, anti-rBL3{gamma} sera did not recognize the IE surfaces of P. falciparum peripheral isolates and strains that do not bind CSA (Fig. 3 and Table 1). Given the questions raised about the role of var1CSA in CSA binding and placental malaria, our confirmation of previous results demonstrating the cross-reactivity of anti-rBL3{gamma} sera with placental isolates is important and reassuring.

However, how does one explain the observed cross-reactivity of anti-rBL3{gamma} sera with CSA-binding placental isolates and laboratory strains, given that they primarily express var2CSA, which does not contain any DBL{gamma} domains? In order to investigate this paradox, we expressed the DBL domains of var2CSA derived from P. falciparum 3D7 on the surface of 293T cells and tested them for binding to CSA and recognition by anti-rBL3{gamma} sera. The DBL2X and DBL3X domains of var2CSA bound CSA in these assays. DNA sequencing confirmed that the sequences of the inserts in the different var2CSA expression constructs used here were identical to the published 3D7 var2CSA sequence. A previous study has reported that the DBL2X and DBL6{varepsilon} domains of 3D7 var2CSA have CSA-binding activity (27). The differences observed between the CSA-binding activity of DBL domains of var2CSA here and that found in the previous study may be due to differences in the domain boundaries of the DBL constructs or differences in the expression systems and cells used for transfection in the two studies. Importantly, we found that anti-rBL3{gamma} sera cross-reacted with the DBL3X domain of var2CSA and blocked the binding of DBL3X with CSA. Anti-rDBL3{gamma} sera did not recognize any of the other DBL domains of var2CSA. The recognition of DBL3X by anti-rDBL3{gamma} sera indicates the presence of common cross-reactive B-cell epitopes in these two diverse CSA-binding DBL domains and explains the paradoxical observations made here and earlier (11, 15, 32) that anti-rDBL3{gamma} sera recognize CSA-binding parasites. We have demonstrated that in addition to recognizing a wide range of placental parasites, anti-rDBL3{gamma} sera block adhesion of IEs to placental cryosections under physiologically relevant flow conditions. This observation indicates that it may be possible to develop prophylactic strategies that block placental sequestration of malaria parasites.

The B-cell epitopes that are recognized by protective antibodies from sera of multigravid women have not yet been identified. Following the identification of var2CSA as the expressed var gene in CSA-binding laboratory strains and placental isolates, recent efforts to identify targets of antibodies that protect against PAM have focused on CSA-binding DBL domains of var2CSA. Here, we have demonstrated that the CSA-binding DBL domains of var1CSA and var2CSA, namely, DBL3{gamma} and DBL3X, respectively, share common B-cell epitopes. Such conserved epitopes that are shared by diverse CSA-binding DBL domains may serve as targets for protective antibodies and form the basis for development of a vaccine against PAM.


   ACKNOWLEDGMENTS
 
We thank Artur Scherf, Institut Pasteur, Paris, France, for providing a plasmid with FCR3 var1CSA gene; Gary Cohen and Roselyn Eisenberg, University of Pennsylvania, Philadelphia, Pennsylvania, for providing plasmid pRE4 and monoclonal antibody DL6; Anup Biswas and Kasturi Datta for providing recombinant gC1qR/HABP1; C. R. Pillai for providing P. falciparum field isolates RAJ68, RAJ104, and JDP8; Iréne Juhan-Vague, Laboratoire d'Hématologie, Marseille, France, for access to FACS; and Catherine Lepolard and Bruno Pouvelle, Université de la Méditerranée, for parasite cultures.

This work was supported by an International Senior Research Fellowship to C.E.C. from the Wellcome Trust, United Kingdom, and by grants to J.G. from BIOMALPAR program, an FP6 funded network of excellence; PAL +2002 of the MENRT; ACI program, INSERM MIC no. 0318; and the Malaria Antigen Discovery Program (MADP) Malaria in Pregnancy Initiative, Bill and Melinda Gates Foundation, no. 29202.


   FOOTNOTES
 
* Corresponding author. Mailing address: Malaria Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi 110067, India. Phone and fax: 91 11 2618 7695. E-mail: cchitnis{at}icgeb.res.in. Back

Editor: J. L. Flynn


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Infection and Immunity, October 2006, p. 5955-5963, Vol. 74, No. 10
0019-9567/06/$08.00+0     doi:10.1128/IAI.00481-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.




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