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Immunization of Pigs To Prevent Disease in Humans: Construction and Protective Efficacy of a Salmonella enterica Serovar Typhimurium Live Negative-Marker Vaccine
Infect. Immun. Selke et al. 75: 2476

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Fig. S1. Coomassie blue-stained 2D-PAGE and Western blot analyses of outer membrane-associated proteins of S. enterica serovar Typhimurium. Fig. S2. Peptide spot array of the OmpD protein and locations of predicted and experimentally determined domains. Fig. S3. Construction of the truncated ompD gene. Fig. S4. Genomic characterization of S. enterica serovar Typhimurium Salmoporc δompD. Fig. S5. Serological responses of pigs in the Salmotype PigScreen ELISA. Table S1. Mass spectrometry results for S. enterica serovar Typhimurium proteins recognized by three porcine sera. Table S2. Antigenic epitopes of the OmpD protein predicted by the HUSAR program ANTIGENIC. Table S3. HMM-predicted structure of the OmpD protein categorized in internal, external, and transmembrane domains. Table S4. Results of the challenge experiment with BALB/c mice. Table S5. Timeline of the vaccination trial in pigs.
    PDF document, 2.3MB.




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