
Soluble CD14 and CD83 from Human Neonatal Antigen-Presenting Cells Are Inducible by Commensal Bacteria and Suppress Allergen-Induced Human Neonatal Th2 Differentiation
Infect. Immun. Lundell et al.
75: 4097
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
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Fig. S1. The release of sCD14 by monocytes and cord blood DC in response to bacterial stimulation was measured.
Zipped PowerPoint presentation, 23K.
- Supplemental file 2
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Fig. S2. Cord blood or monocytes were stimulated with UV-killed gram-positive or gram-negative bacteria for 24 or 48 h, and sCD83 levels in the supernatant were analyzed by ELISA.
Zipped PowerPoint presentation, 37K.
- Supplemental file 3
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Fig. S3. Cord blood DC were stimulated with UV-killed gram-positive or gram-negative bacteria for 24 or 48 h, and the levels of CD14 and CD83 expression on the cell surface were analyzed by flow cytometry.
Zipped PowerPoint presentation, 49K.
- Supplemental file 4
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Fig. S4. LPS or PGN does not interfere with the sCD14 ELISA.
Zipped PowerPoint presentation, 9K.
- Supplemental file 5
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Fig. S5. CD14 is not expressed intracellularly in cord blood DC.
Zipped PowerPoint presentation, 24K.
- Supplemental file 6
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Fig. S6. Production levels of IL-5 and IFN-γ were analyzed by ELISA.
Zipped PowerPoint presentation, 14K.
- Supplemental file 7
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Table S1. Percentages of CD3-positive T cells expressing IL-13 intracellularly were measured.
MS Word document, 31K.
- Supplemental file 8
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Legends.doc. Legends for Fig. S1 to S6.
MS Word document, 29K.