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Soluble CD14 and CD83 from Human Neonatal Antigen-Presenting Cells Are Inducible by Commensal Bacteria and Suppress Allergen-Induced Human Neonatal Th2 Differentiation
Infect. Immun. Lundell et al. 75: 4097

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Fig. S1. The release of sCD14 by monocytes and cord blood DC in response to bacterial stimulation was measured.
    Zipped PowerPoint presentation, 23K.
  • Supplemental file 2 - Fig. S2. Cord blood or monocytes were stimulated with UV-killed gram-positive or gram-negative bacteria for 24 or 48 h, and sCD83 levels in the supernatant were analyzed by ELISA.
    Zipped PowerPoint presentation, 37K.
  • Supplemental file 3 - Fig. S3. Cord blood DC were stimulated with UV-killed gram-positive or gram-negative bacteria for 24 or 48 h, and the levels of CD14 and CD83 expression on the cell surface were analyzed by flow cytometry.
    Zipped PowerPoint presentation, 49K.
  • Supplemental file 4 - Fig. S4. LPS or PGN does not interfere with the sCD14 ELISA.
    Zipped PowerPoint presentation, 9K.
  • Supplemental file 5 - Fig. S5. CD14 is not expressed intracellularly in cord blood DC.
    Zipped PowerPoint presentation, 24K.
  • Supplemental file 6 - Fig. S6. Production levels of IL-5 and IFN-γ were analyzed by ELISA.
    Zipped PowerPoint presentation, 14K.
  • Supplemental file 7 - Table S1. Percentages of CD3-positive T cells expressing IL-13 intracellularly were measured.
    MS Word document, 31K.
  • Supplemental file 8 - Legends.doc. Legends for Fig. S1 to S6.
    MS Word document, 29K.




This Article
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