In Vitro Analysis of Tobramycin-Treated Pseudomonas aeruginosa Biofilms on Cystic Fibrosis-Derived Airway Epithelial Cells
Infect. Immun. Anderson et al.
76: 1423
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
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Table S1. Strains and plasmids used in the study.
Zipped MS Word document 10kb
- Supplemental file 2
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Table S2. Primers used in the study.
Zipped MS Word document 8kb
- Supplemental file 3
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Table S3. Genes upregulated in CFBE-grown biofilms 30 min after TB treatment.
Zipped MS Excel document 21kb
- Supplemental file 4
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Table S4. Genes downregulated in CFBE-grown biofilms 30 min after TB treatment.
Zipped MS Excel document 34kb
- Supplemental file 5
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Table S5. Genes upregulated in planktonically grown bacteria 30 min after TB treatment.
Zipped MS Excel document 5kb
- Supplemental file 6
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Table S6. Genes downregulated in planktonically grown bacteria 30 min after TB treatment.
Zipped MS Excel document 4kb
- Supplemental file 7
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Table S7. Comparison of tobramycin-induced transcription in CFBE biofilm, abiotic biofilm, and planktonic microarrays.
Zipped MS Word document 4kb
- Supplemental file 8
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Table S8. Phenotypes of mutant genes identified in microarray studies.
Zipped MS Word document 5kb
- Supplemental file 9
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Fig. S1. Venn analysis of microarray data from planktonic cultures, CFBE-PA14 biofilms, and abiotic biofilms treated with tobramycin.
Fig. S2. qRT-PCR analysis of selected genes in P. aeruginosa grown as abiotic biofilms and biofilms on CFBE cells.
Fig. S3. Cytotoxicity assay controls.
Zipped PPT document 80kb
- Supplemental file 10
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Legends for Fig. S1 to S3.
Zipped MS Word document 4kb
