IAI FigSearch
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jenkins, E. M.
Right arrow Articles by Watson, B. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jenkins, E. M.
Right arrow Articles by Watson, B. B.

 Previous Article  |  Next Article 

Infect Immun. 1971 April; 3(4): 589-594
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Extracellular Antigens from Listeria monocytogenes I. Purification and Resolution of Hemolytic and Lipolytic Antigens from Culture Filtrates of Listeria monocytogenes

E. M. Jenkins and B. B. Watson

Department of Microbiology, School of Veterinary Medicine, Tuskegee Institute, Alabama 36088

ABSTRACT

Two antigens were purified from culture filtrates of Listeria monocytogenes 7973 by the following procedure: (i) acid precipitation with 4 N HCl at pH 3.7, (ii) Sephadex G-75 column fractionation, (iii) diethylaminoethyl-Sephadex A50 batchwise adsorption, and (iv) rechromatography on Sephadex G-75. This procedure resulted in the resolution of two distinct antigens. One antigen, designated a hemolytic antigen because of its ability to lyse erythrocytes from a variety of species, had a specific activity of 25,000 units/mg of protein and an estimated molecular weight of at least 171,000. The other antigen, designated a lipolytic antigen because of its ability to hydrolyze egg yolk saline substrate, had a specific activity of 400 units/mg of protein and an estimated molecular weight of 52,500.

Infect Immun. 1971 April; 3(4): 589-594
Copyright © 1971 American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 1971 by the American Society for Microbiology. All rights reserved.