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Infect Immun. 1991 October; 59(10): 3407-3410

Contribution of the B oligomer to the protective activity of genetically attenuated pertussis toxin.

Division of Bacterial Products, Center for Biologics Evaluation and Research, Bethesda, Maryland 20892.

ABSTRACT

An enzymatically deficient recombinant S1 subunit, in which Arg-9 was replaced by Lys, was combined with native B oligomer to form a mutant holotoxin molecule. This molecule exhibited decreased leukocytosis-promoting and histamine-sensitizing activities compared with those of the native toxin, supporting the view that the B oligomer is not responsible for these activities. The protective activity of this genetically attenuated pertussis toxin was compared with that of B oligomer alone. The mutant pertussis toxin and B oligomer were similarly capable of protecting mice against a respiratory infection with Bordetella pertussis, suggesting that the B oligomer makes a significant contribution to the protection afforded by the genetically attenuated holotoxin.

This article has been cited by other articles:

• Hausman, S. Z., Burns, D. L. (2000). Use of Pertussis Toxin Encoded by ptx Genes from Bordetella bronchiseptica To Model the Effects of Antigenic Drift of Pertussis Toxin on Antibody Neutralization. Infect. Immun. 68: 3763-3767 [Abstract] [Full Text]