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Infect Immun. 1991 May; 59(5): 1627-1632

Synthesis of tetanus toxin fragment C in insect cells by use of a baculovirus expression system.

Department of Molecular Biology, Wellcome Research Laboratories, Beckenham, Kent, England.

ABSTRACT

The baculovirus expression vector p36C was used to express in cells of the insect Spodoptera frugiperda fragment C of tetanus toxin under the control of the strong polyhedrin promoter. Fragment C was expressed intracellularly at a high level and was soluble, allowing it to be purified by affinity chromatography with monoclonal antibody TT08. Purified fragment C from baculovirus was used to immunize mice and was shown to successfully prevent the symptoms of tetanus following a toxin challenge. The ganglioside-binding properties of baculovirus-derived fragment C were compared with those of intact tetanus toxin and native fragment C and were found to be dissimilar.

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