This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kreft, B Articles by Wirth, R Search for Related Content PubMed PubMed Citation Articles by Kreft, B Articles by Wirth, R

 Previous Article  |  Next Article 

Infect Immun. 1992 January; 60(1): 25-30

Aggregation substance of Enterococcus faecalis mediates adhesion to cultured renal tubular cells.

Klinik für Innere Medizin, Medizinische Universität zu Lübeck, Germany.

ABSTRACT

The sex pheromone system of Enterococcus faecalis is a unique, highly efficient plasmid collection mechanism for this species. A crucial role in this system is played by an adhesin called aggregation substance which enables the cell-cell contact between donor and recipient strains. The existence of the amino acid motif Arg-Gly-Asp-Ser in the adhesin prompted us to look for a possible binding of E. faecalis cells expressing aggregation substance to eucaryotic cells. We were able to show that the adhesin mediated binding to cultured renal tubular cells (porcine cell line LLC-PK1) via light microscopic, electron microscopic, and enzyme-linked immunosorbent assay-based studies. Synthesis of the adhesin was induced by some component(s) of serum. These data are interpreted to mean that aggregation substance is an adhesin mediating not only cell-cell contact between different E. faecalis strains but also binding of E. faecalis to eucaryotic cells, and therefore it might contribute to virulence.

This article has been cited by other articles:

• Tsigrelis, C., Singh, K. V., Coutinho, T. D., Murray, B. E., Baddour, L. M. (2007). Vancomycin-Resistant Enterococcus faecalis Endocarditis: Linezolid Failure and Strain Characterization of Virulence Factors. J. Clin. Microbiol. 45: 631-635 [Abstract] [Full Text]  
• Nallapareddy, S. R., Murray, B. E. (2006). Ligand-Signaled Upregulation of Enterococcus faecalis ace Transcription, a Mechanism for Modulating Host-E. faecalis Interaction. Infect. Immun. 74: 4982-4989 [Abstract] [Full Text]  
• Chandler, J. R., Hirt, H., Dunny, G. M. (2005). A paracrine peptide sex pheromone also acts as an autocrine signal to induce plasmid transfer and virulence factor expression in vivo. Proc. Natl. Acad. Sci. USA 102: 15617-15622 [Abstract] [Full Text]  
• Hirt, H., Manias, D. A., Bryan, E. M., Klein, J. R., Marklund, J. K., Staddon, J. H., Paustian, M. L., Kapur, V., Dunny, G. M. (2005). Characterization of the Pheromone Response of the Enterococcus faecalis Conjugative Plasmid pCF10: Complete Sequence and Comparative Analysis of the Transcriptional and Phenotypic Responses of pCF10-Containing Cells to Pheromone Induction. J. Bacteriol. 187: 1044-1054 [Abstract] [Full Text]  
• Tomita, H., Ike, Y. (2004). Tissue-Specific Adherent Enterococcus faecalis Strains That Show Highly Efficient Adhesion to Human Bladder Carcinoma T24 Cells Also Adhere to Extracellular Matrix Proteins. Infect. Immun. 72: 5877-5885 [Abstract] [Full Text]  
• Vankerckhoven, V., Van Autgaerden, T., Vael, C., Lammens, C., Chapelle, S., Rossi, R., Jabes, D., Goossens, H. (2004). Development of a Multiplex PCR for the Detection of asa1, gelE, cylA, esp, and hyl Genes in Enterococci and Survey for Virulence Determinants among European Hospital Isolates of Enterococcus faecium. J. Clin. Microbiol. 42: 4473-4479 [Abstract] [Full Text]  
• Kayaoglu, G., Orstavik, D. (2004). VIRULENCE FACTORS OF ENTEROCOCCUS FAECALIS: RELATIONSHIP TO ENDODONTIC DISEASE. Crit. Rev. Oral Biol. Med. 15: 308-320 [Abstract] [Full Text]  
• Donelli, G., Paoletti, C., Baldassarri, L., Guaglianone, E., Di Rosa, R., Magi, G., Spinaci, C., Facinelli, B. (2004). Sex Pheromone Response, Clumping, and Slime Production in Enterococcal Strains Isolated from Occluded Biliary Stents. J. Clin. Microbiol. 42: 3419-3427 [Abstract] [Full Text]  
• Stentz, R., Jury, K., Eaton, T., Parker, M., Narbad, A., Gasson, M., Shearman, C. (2004). Controlled expression of CluA in Lactococcus lactis and its role in conjugation. Microbiology 150: 2503-2512 [Abstract] [Full Text]  
• Johnson, J. R., Clabots, C., Hirt, H., Waters, C., Dunny, G. (2004). Enterococcal Aggregation Substance and Binding Substance Are Not Major Contributors to Urinary Tract Colonization by Enterococcus faecalis in a Mouse Model of Ascending Unobstructed Urinary Tract Infection. Infect. Immun. 72: 2445-2448 [Abstract] [Full Text]  
• Waters, C. M., Wells, C. L., Dunny, G. M. (2003). The Aggregation Domain of Aggregation Substance, Not the RGD Motifs, Is Critical for Efficient Internalization by HT-29 Enterocytes. Infect. Immun. 71: 5682-5689 [Abstract] [Full Text]  
• Teng, F., Kawalec, M., Weinstock, G. M., Hryniewicz, W., Murray, B. E. (2003). An Enterococcus faecium Secreted Antigen, SagA, Exhibits Broad-Spectrum Binding to Extracellular Matrix Proteins and Appears Essential for E. faecium Growth. Infect. Immun. 71: 5033-5041 [Abstract] [Full Text]  
• Dupre, I., Zanetti, S., Schito, A. M., Fadda, G., Sechi, L. A. (2003). Incidence of virulence determinants in clinical Enterococcus faecium and Enterococcus faecalis isolates collected in Sardinia (Italy). J Med Microbiol 52: 491-498 [Abstract] [Full Text]  
• Magi, G., Capretti, R., Paoletti, C., Pietrella, M., Ferrante, L., Biavasco, F., Varaldo, P. E., Facinelli, B. (2003). Presence of a vanA-Carrying Pheromone Response Plasmid (pBRG1) in a Clinical Isolate of Enterococcus faecium. Antimicrob. Agents Chemother. 47: 1571-1576 [Abstract] [Full Text]  
• Gentry-Weeks, C., Estay, M., Loui, C., Baker, D. (2003). Intravenous Mouse Infection Model for Studying the Pathology of Enterococcus faecalis Infections. Infect. Immun. 71: 1434-1441 [Abstract] [Full Text]  
• Shepard, B. D., Gilmore, M. S. (2002). Differential Expression of Virulence-Related Genes in Enterococcus faecalis in Response to Biological Cues in Serum and Urine. Infect. Immun. 70: 4344-4352 [Abstract] [Full Text]  
• Waar, K., van der Mei, H. C., Harmsen, H. J. M., Degener, J. E., Busscher, H. J. (2002). Enterococcus faecalis surface proteins determine its adhesion mechanism to bile drain materials. Microbiology 148: 1863-1870 [Abstract] [Full Text]  
• An, F. Y., Clewell, D. B. (2002). Identification of the cAD1 Sex Pheromone Precursor in Enterococcus faecalis. J. Bacteriol. 184: 1880-1887 [Abstract] [Full Text]  
• Hirt, H., Schlievert, P. M., Dunny, G. M. (2002). In Vivo Induction of Virulence and Antibiotic Resistance Transfer in Enterococcus faecalis Mediated by the Sex Pheromone-Sensing System of pCF10. Infect. Immun. 70: 716-723 [Abstract] [Full Text]  
• Waters, C. M., Dunny, G. M. (2001). Analysis of Functional Domains of the Enterococcus faecalis Pheromone-Induced Surface Protein Aggregation Substance. J. Bacteriol. 183: 5659-5667 [Abstract] [Full Text]  
• Garsin, D. A., Sifri, C. D., Mylonakis, E., Qin, X., Singh, K. V., Murray, B. E., Calderwood, S. B., Ausubel, F. M. (2001). A simple model host for identifying Gram-positive virulence factors. Proc. Natl. Acad. Sci. USA 10.1073/pnas.191378698v1 [Abstract] [Full Text]  
• Shankar, N., Lockatell, C. V., Baghdayan, A. S., Drachenberg, C., Gilmore, M. S., Johnson, D. E. (2001). Role of Enterococcus faecalis Surface Protein Esp in the Pathogenesis of Ascending Urinary Tract Infection. Infect. Immun. 69: 4366-4372 [Abstract] [Full Text]  
• McCormick, J. K., Hirt, H., Waters, C. M., Tripp, T. J., Dunny, G. M., Schlievert, P. M. (2001). Antibodies to a Surface-Exposed, N-terminal Domain of Aggregation Substance Are Not Protective in the Rabbit Model of Enterococcus faecalis Infective Endocarditis. Infect. Immun. 69: 3305-3314 [Abstract] [Full Text]  
• Eaton, T. J., Gasson, M. J. (2001). Molecular Screening of Enterococcus Virulence Determinants and Potential for Genetic Exchange between Food and Medical Isolates. Appl. Environ. Microbiol. 67: 1628-1635 [Abstract] [Full Text]  
• Wells, C. L., Moore, E. A., Hoag, J. A., Hirt, H., Dunny, G. M., Erlandsen, S. L. (2000). Inducible Expression of Enterococcus faecalis Aggregation Substance Surface Protein Facilitates Bacterial Internalization by Cultured Enterocytes. Infect. Immun. 68: 7190-7194 [Abstract] [Full Text]  
• Mundy, L. M., Sahm, D. F., Gilmore, M. (2000). Relationships between Enterococcal Virulence and Antimicrobial Resistance. Clin. Microbiol. Rev. 13: 513-522 [Abstract] [Full Text]  
• Sartingen, S., Rozdzinski, E., Muscholl-Silberhorn, A., Marre, R. (2000). Aggregation Substance Increases Adherence and Internalization, but Not Translocation, of Enterococcus faecalis through Different Intestinal Epithelial Cells In Vitro. Infect. Immun. 68: 6044-6047 [Abstract] [Full Text]  
• Su{beta}muth, S. D., Muscholl-Silberhorn, A., Wirth, R., Susa, M., Marre, R., Rozdzinski, E. (2000). Aggregation Substance Promotes Adherence, Phagocytosis, and Intracellular Survival of Enterococcus faecalis within Human Macrophages and Suppresses Respiratory Burst. Infect. Immun. 68: 4900-4906 [Abstract] [Full Text]  
• Huebner, J., Quaas, A., Krueger, W. A., Goldmann, D. A., Pier, G. B. (2000). Prophylactic and Therapeutic Efficacy of Antibodies to a Capsular Polysaccharide Shared among Vancomycin-Sensitive and -Resistant Enterococci. Infect. Immun. 68: 4631-4636 [Abstract] [Full Text]  
• Muscholl-Silberhorn, A. B. (2000). Pheromone-Regulated Expression of Sex Pheromone Plasmid pAD1-Encoded Aggregation Substance Depends on at Least Six Upstream Genes and a cis-Acting, Orientation-Dependent Factor. J. Bacteriol. 182: 3816-3825 [Abstract] [Full Text]  
• Hirt, H., Erlandsen, S. L., Dunny, G. M. (2000). Heterologous Inducible Expression of Enterococcus faecalis pCF10 Aggregation Substance Asc10 in Lactococcus lactis and Streptococcus gordonii Contributes to Cell Hydrophobicity and Adhesion to Fibrin. J. Bacteriol. 182: 2299-2306 [Abstract] [Full Text]  
• Rakita, R. M., Vanek, N. N., Jacques-Palaz, K., Mee, M., Mariscalco, M. M., Dunny, G. M., Snuggs, M., Van Winkle, W. B., Simon, S. I. (1999). Enterococcus faecalis Bearing Aggregation Substance Is Resistant to Killing by Human Neutrophils despite Phagocytosis and Neutrophil Activation. Infect. Immun. 67: 6067-6075 [Abstract] [Full Text]  
• Gentry-Weeks, C. R., Karkhoff-Schweizer, R., Pikis, A., Estay, M., Keith, J. M. (1999). Survival of Enterococcus faecalis in Mouse Peritoneal Macrophages. Infect. Immun. 67: 2160-2165 [Abstract] [Full Text]  
• Shepard, B. D., Gilmore, M. S. (1999). Identification of Aerobically and Anaerobically Induced Genes in Enterococcus faecalis by Random Arbitrarily Primed PCR. Appl. Environ. Microbiol. 65: 1470-1476 [Abstract] [Full Text]  
• Shiono, A., Ike, Y. (1999). Isolation of Enterococcus faecalis Clinical Isolates That Efficiently Adhere to Human Bladder Carcinoma T24 Cells and Inhibition of Adhesion by Fibronectin and Trypsin Treatment. Infect. Immun. 67: 1585-1592 [Abstract] [Full Text]  
• Shankar, V., Baghdayan, A. S., Huycke, M. M., Lindahl, G., Gilmore, M. S. (1999). Infection-Derived Enterococcus faecalis Strains Are Enriched in esp, a Gene Encoding a Novel Surface Protein. Infect. Immun. 67: 193-200 [Abstract] [Full Text]  
• Holmes, A. R., Gilbert, C., Wells, J. M., Jenkinson, H. F. (1998). Binding Properties of Streptococcus gordonii SspA and SspB (Antigen I/II Family) Polypeptides Expressed on the Cell Surface of Lactococcus lactis MG1363. Infect. Immun. 66: 4633-4639 [Abstract] [Full Text]  
• Dupont, H., Montravers, P., Mohler, J., Carbon, C. (1998). Disparate Findings on the Role of Virulence Factors of Enterococcus faecalis in Mouse and Rat Models of Peritonitis. Infect. Immun. 66: 2570-2575 [Abstract] [Full Text]  
• Fujimoto, S., Clewell, D. B. (1998). Regulation of the pAD1 sex pheromone response of Enterococcus faecalis by direct interaction between the cAD1 peptide mating signal and the negatively regulating, DNA-binding TraA protein. Proc. Natl. Acad. Sci. USA 95: 6430-6435 [Abstract] [Full Text]  
• Jett, B. D., Atkuri, R. V., Gilmore, M. S. (1998). Enterococcus faecalis Localization in Experimental Endophthalmitis: Role of Plasmid-Encoded Aggregation Substance. Infect. Immun. 66: 843-848 [Abstract] [Full Text]  
• Schlievert, P. M., Gahr, P. J., Assimacopoulos, A. P., Dinges, M. M., Stoehr, J. A., Harmala, J. W., Hirt, H., Dunny, G. M. (1998). Aggregation and Binding Substances Enhance Pathogenicity in Rabbit Models of Enterococcus faecalis Endocarditis. Infect. Immun. 66: 218-223 [Abstract] [Full Text]  
• Garsin, D. A., Sifri, C. D., Mylonakis, E., Qin, X., Singh, K. V., Murray, B. E., Calderwood, S. B., Ausubel, F. M. (2001). A simple model host for identifying Gram-positive virulence factors. Proc. Natl. Acad. Sci. USA 98: 10892-10897 [Abstract] [Full Text]