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Infect Immun. 1992 January; 60(1): 278-283

Immunochemical studies and complete amino acid sequence of the streptokinase from Streptococcus pyogenes (group A) M type 12 strain A374.

H Ohkuni, Y Todome, H Suzuki, M Mizuse, N Kotani, K Horiuchi, N Shikama, A Tsugita and K H Johnston

Division of Immunology, Nippon Medical School, Kanagawa, Japan.

ABSTRACT

The complete amino acid sequence of the streptokinase (SKase) of Streptococcus pyogenes M type 12 strain A374, isolated from a patient with poststreptococcal glomerulonephritis (PSGN), was determined. The epitope domain for the monoclonal antibody N-59, which cross-reacts with SKases of both the PSGN-associated strain and S. equisimilis H46A (a non-PSGN-associated strain), was predicted to be localized in residues 370 to 374. The epitope domain specific for monoclonal antibody RU-1, which reacts only with the PSGN-associated SKase, was localized to residues 164 to 236.

Infect Immun. 1992 January; 60(1): 278-283




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