This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gentschev, I Articles by Goebel, W Search for Related Content PubMed PubMed Citation Articles by Gentschev, I Articles by Goebel, W

 Previous Article  |  Next Article 

Infect Immun. 1992 December; 60(12): 5091-5098

Identification of p60 antibodies in human sera and presentation of this listerial antigen on the surface of attenuated salmonellae by the HlyB-HlyD secretion system.

Lehrstuhl für Mikrobiologie, Theodor-Boveri-Institut für Biowissenschaften (Biozentrum), Universität Würzburg, Germany.

ABSTRACT

Antibodies directed against the major secreted protein of Listeria monocytogenes, termed p60, were found more frequently than antilisteriolysin antibodies in sera of listeriosis patients. Anti-p60 antibodies were also identified in all tested sera from healthy individuals. To test whether p60 provides protection against L. monocytogenes, we constructed an attenuated Salmonella typhimurium aroA strain which secretes p60 via the Escherichia coli hemolysin secretion pathway. Application of this Salmonella strain to BALB/c mice prior to an L. monocytogenes infection induced p60 antibodies in these mice and led to a significantly reduced number of viable bacteria in the spleen compared with that in control animals which were primed with the S. typhimurium aroA strain alone.

This article has been cited by other articles:

• Yu, W. L., Dan, H., Lin, M. (2007). Novel protein targets of the humoral immune response to Listeria monocytogenes infection in rabbits. J Med Microbiol 56: 888-895 [Abstract] [Full Text]  
• Yu, K.-Y., Noh, Y., Chung, M., Park, H.-J., Lee, N., Youn, M., Jung, B. Y., Youn, B.-S. (2004). Use of Monoclonal Antibodies That Recognize p60 for Identification of Listeria monocytogenes. CVI 11: 446-451 [Abstract] [Full Text]  
• Drevets, D. A., Leenen, P. J. M., Greenfield, R. A. (2004). Invasion of the Central Nervous System by Intracellular Bacteria. Clin. Microbiol. Rev. 17: 323-347 [Abstract] [Full Text]  
• Pilgrim, S., Kolb-Maurer, A., Gentschev, I., Goebel, W., Kuhn, M. (2003). Deletion of the Gene Encoding p60 in Listeria monocytogenes Leads to Abnormal Cell Division and Loss of Actin-Based Motility. Infect. Immun. 71: 3473-3484 [Abstract] [Full Text]  
• Hertzig, T., Weber, M., Greiffenberg, L., Holthausen, B. S., Goebel, W., Kim, K. S., Kuhn, M. (2003). Antibodies Present in Normal Human Serum Inhibit Invasion of Human Brain Microvascular Endothelial Cells by Listeria monocytogenes. Infect. Immun. 71: 95-100 [Abstract] [Full Text]  
• Manohar, M., Baumann, D. O., Bos, N. A., Cebra, J. J. (2001). Gut Colonization of Mice with actA-Negative Mutant of Listeria monocytogenes Can Stimulate a Humoral Mucosal Immune Response. Infect. Immun. 69: 3542-3549 [Abstract] [Full Text]  
• Kolb-Maurer, A., Pilgrim, S., Kampgen, E., McLellan, A. D., Brocker, E.-B., Goebel, W., Gentschev, I. (2001). Antibodies against Listerial Protein 60 Act as an Opsonin for Phagocytosis of Listeria monocytogenes by Human Dendritic Cells. Infect. Immun. 69: 3100-3109 [Abstract] [Full Text]  
• Vijh, S., Pilip, I. M., Pamer, E. G. (1999). Noncompetitive Expansion of Cytotoxic T Lymphocytes Specific for Different Antigens during Bacterial Infection. Infect. Immun. 67: 1303-1309 [Abstract] [Full Text]  
• Gentschev, I., Glaser, I., Goebel, W., McKeever, D. J., Musoke, A., Heussler, V. T. (1998). Delivery of the p67 Sporozoite Antigen of Theileria parva by Using Recombinant Salmonella dublin: Secretion of the Product Enhances Specific Antibody Responses in Cattle. Infect. Immun. 66: 2060-2064 [Abstract] [Full Text]