This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rubens, C E Articles by van Belle, G Search for Related Content PubMed PubMed Citation Articles by Rubens, C E Articles by van Belle, G

 Previous Article  |  Next Article 

Infect Immun. 1992 December; 60(12): 5157-5163

Respiratory epithelial cell invasion by group B streptococci.

Department of Pediatrics, Children's Hospital and Medical Center, University of Washington, Seattle 98105.

ABSTRACT

Group B streptococci (GBS) are the most common cause of pneumonia and sepsis during the neonatal period; however, the pathogenesis of this infection is poorly understood. We investigated the ability of GBS to enter epithelial cells in culture. Two strains of GBS were capable of invading immortalized respiratory epithelial cell lines in vitro at different levels, suggesting strain differences in invasiveness. Intracellular replication was not observed. Invasion required actin microfilaments but not microtubular cytoskeletal elements. Active bacterial protein, DNA, and RNA syntheses were required for invasion. These findings are consistent with our previous observation of intracellular GBS in the lungs of infected primates. We hypothesize that this organism may access the bloodstream by direct invasion of the epithelial cell barrier.

This article has been cited by other articles:

• Burnham, C.-A. D., Shokoples, S. E., Tyrrell, G. J. (2007). Invasion of HeLa cells by group B streptococcus requires the phosphoinositide-3-kinase signalling pathway and modulates phosphorylation of host-cell Akt and glycogen synthase kinase-3. Microbiology 153: 4240-4252 [Abstract] [Full Text]  
• Fettucciari, K., Fetriconi, I., Mannucci, R., Nicoletti, I., Bartoli, A., Coaccioli, S., Marconi, P. (2006). Group B streptococcus induces macrophage apoptosis by calpain activation.. J. Immunol. 176: 7542-7556 [Abstract] [Full Text]  
• Tenenbaum, T., Bloier, C., Adam, R., Reinscheid, D. J., Schroten, H. (2005). Adherence to and Invasion of Human Brain Microvascular Endothelial Cells Are Promoted by Fibrinogen-Binding Protein FbsA of Streptococcus agalactiae. Infect. Immun. 73: 4404-4409 [Abstract] [Full Text]  
• Lalioui, L., Pellegrini, E., Dramsi, S., Baptista, M., Bourgeois, N., Doucet-Populaire, F., Rusniok, C., Zouine, M., Glaser, P., Kunst, F., Poyart, C., Trieu-Cuot, P. (2005). The SrtA Sortase of Streptococcus agalactiae Is Required for Cell Wall Anchoring of Proteins Containing the LPXTG Motif, for Adhesion to Epithelial Cells, and for Colonization of the Mouse Intestine. Infect. Immun. 73: 3342-3350 [Abstract] [Full Text]  
• Auperin, T. C., Bolduc, G. R., Baron, M. J., Heroux, A., Filman, D. J., Madoff, L. C., Hogle, J. M. (2005). Crystal Structure of the N-terminal Domain of the Group B Streptococcus Alpha C Protein. J. Biol. Chem. 280: 18245-18252 [Abstract] [Full Text]  
• Hakansson, A., Bentley, C. C., Shakhnovic, E. A., Wessels, M. R. (2005). Cytolysin-dependent evasion of lysosomal killing. Proc. Natl. Acad. Sci. USA 102: 5192-5197 [Abstract] [Full Text]  
• Seepersaud, R., Hanniffy, S. B., Mayne, P., Sizer, P., Le Page, R., Wells, J. M. (2005). Characterization of a Novel Leucine-Rich Repeat Protein Antigen from Group B Streptococci That Elicits Protective Immunity. Infect. Immun. 73: 1671-1683 [Abstract] [Full Text]  
• Sukhnanand, S., Dogan, B., Ayodele, M. O., Zadoks, R. N., Craver, M. P. J., Dumas, N. B., Schukken, Y. H., Boor, K. J., Wiedmann, M. (2005). Molecular Subtyping and Characterization of Bovine and Human Streptococcus agalactiae Isolates. J. Clin. Microbiol. 43: 1177-1186 [Abstract] [Full Text]  
• Lindahl, G., Stalhammar-Carlemalm, M., Areschoug, T. (2005). Surface Proteins of Streptococcus agalactiae and Related Proteins in Other Bacterial Pathogens. Clin. Microbiol. Rev. 18: 102-127 [Abstract] [Full Text]  
• Mikamo, H., Johri, A. K., Paoletti, L. C., Madoff, L. C., Onderdonk, A. B. (2004). Adherence to, Invasion by, and Cytokine Production in Response to Serotype VIII Group B Streptococci. Infect. Immun. 72: 4716-4722 [Abstract] [Full Text]  
• Baron, M. J., Bolduc, G. R., Goldberg, M. B., Auperin, T. C., Madoff, L. C. (2004). Alpha C Protein of Group B Streptococcus Binds Host Cell Surface Glycosaminoglycan and Enters Cells by an Actin-dependent Mechanism. J. Biol. Chem. 279: 24714-24723 [Abstract] [Full Text]  
• Gutekunst, H., Eikmanns, B. J., Reinscheid, D. J. (2004). The Novel Fibrinogen-Binding Protein FbsB Promotes Streptococcus agalactiae Invasion into Epithelial Cells. Infect. Immun. 72: 3495-3504 [Abstract] [Full Text]  
• Johri, A. K., Padilla, J., Malin, G., Paoletti, L. C. (2003). Oxygen Regulates Invasiveness and Virulence of Group B Streptococcus. Infect. Immun. 71: 6707-6711 [Abstract] [Full Text]  
• Adderson, E. E., Takahashi, S., Wang, Y., Armstrong, J., Miller, D. V., Bohnsack, J. F. (2003). Subtractive Hybridization Identifies a Novel Predicted Protein Mediating Epithelial Cell Invasion by Virulent Serotype III Group B Streptococcus agalactiae. Infect. Immun. 71: 6857-6863 [Abstract] [Full Text]  
• Gutekunst, H., Eikmanns, B. J., Reinscheid, D. J. (2003). Analysis of RogB-Controlled Virulence Mechanisms and Gene Expression in Streptococcus agalactiae. Infect. Immun. 71: 5056-5064 [Abstract] [Full Text]  
• Zlotkin, A., Chilmonczyk, S., Eyngor, M., Hurvitz, A., Ghittino, C., Eldar, A. (2003). Trojan Horse Effect: Phagocyte-Mediated Streptococcus iniae Infection of Fish. Infect. Immun. 71: 2318-2325 [Abstract] [Full Text]  
• Stinson, M. W., Alder, S., Kumar, S. (2003). Invasion and Killing of Human Endothelial Cells by Viridans Group Streptococci. Infect. Immun. 71: 2365-2372 [Abstract] [Full Text]  
• Tyrrell, G. J., Kennedy, A., Shokoples, S. E., Sherburne, R. K. (2002). Binding and invasion of HeLa and MRC-5 cells by Streptococcus agalactiae. Microbiology 148: 3921-3931 [Abstract] [Full Text]  
• Tamura, G. S., Nittayajarn, A., Schoentag, D. L. (2002). A Glutamine Transport Gene, glnQ, Is Required for Fibronectin Adherence and Virulence of Group B Streptococci. Infect. Immun. 70: 2877-2885 [Abstract] [Full Text]  
• Cheng, Q., Stafslien, D., Purushothaman, S. S., Cleary, P. (2002). The Group B Streptococcal C5a Peptidase Is Both a Specific Protease and an Invasin. Infect. Immun. 70: 2408-2413 [Abstract] [Full Text]  
• Cortes, G., Alvarez, D., Saus, C., Alberti, S. (2002). Role of Lung Epithelial Cells in Defense against Klebsiella pneumoniae Pneumonia. Infect. Immun. 70: 1075-1080 [Abstract] [Full Text]  
• Goodrum, K. J., Poulson-Dunlap, J. (2002). Cytokine Responses to Group B Streptococci Induce Nitric Oxide Production in Respiratory Epithelial Cells. Infect. Immun. 70: 49-54 [Abstract] [Full Text]  
• Poyart, C., Lamy, M. C., Boumaila, C., Fiedler, F., Trieu-Cuot, P. (2001). Regulation of D-Alanyl-Lipoteichoic Acid Biosynthesis in Streptococcus agalactiae Involves a Novel Two-Component Regulatory System. J. Bacteriol. 183: 6324-6334 [Abstract] [Full Text]  
• Malin, G., Paoletti, L. C. (2001). Use of a dynamic in vitro attachment and invasion system (DIVAS) to determine influence of growth rate on invasion of respiratory epithelial cells by group B Streptococcus. Proc. Natl. Acad. Sci. USA 10.1073/pnas.241079098v1 [Abstract] [Full Text]  
• Fuller, J. D., Bast, D. J., Nizet, V., Low, D. E., de Azavedo, J. C. S. (2001). Streptococcus iniae Virulence Is Associated with a Distinct Genetic Profile. Infect. Immun. 69: 1994-2000 [Abstract] [Full Text]  
• Stearns, R. C., Paulauskis, J. D., Godleski, J. J. (2001). Endocytosis of Ultrafine Particles by A549 Cells. Am. J. Respir. Cell Mol. Bio. 24: 108-115 [Abstract] [Full Text]  
• Orihuela, C. J., Janssen, R., Robb, C. W., Watson, D. A., Niesel, D. W. (2000). Peritoneal Culture Alters Streptococcus pneumoniae Protein Profiles and Virulence Properties. Infect. Immun. 68: 6082-6086 [Abstract] [Full Text]  
• Fettucciari, K., Rosati, E., Scaringi, L., Cornacchione, P., Migliorati, G., Sabatini, R., Fetriconi, I., Rossi, R., Marconi, P. (2000). Group B Streptococcus Induces Apoptosis in Macrophages. J. Immunol. 165: 3923-3933 [Abstract] [Full Text]  
• Kahl, B. C., Goulian, M., van Wamel, W., Herrmann, M., Simon, S. M., Kaplan, G., Peters, G., Cheung, A. L. (2000). Staphylococcus aureus RN6390 Replicates and Induces Apoptosis in a Pulmonary Epithelial Cell Line. Infect. Immun. 68: 5385-5392 [Abstract] [Full Text]  
• Lalonde, M., Segura, M., Lacouture, S., Gottschalk, M. (2000). Interactions between Streptococcus suis serotype 2 and different epithelial cell lines. Microbiology 146: 1913-1921 [Abstract] [Full Text]  
• Tamura, G. S., Nittayajarn, A. (2000). Group B Streptococci and Other Gram-Positive Cocci Bind to Cytokeratin 8. Infect. Immun. 68: 2129-2134 [Abstract] [Full Text]  
• López-Dóriga, M. V., Barnes, A. C., dos Santos, N. M. S., Ellis, A. E. (2000). Invasion of fish epithelial cells by Photobacterium damselae subsp. piscicida: evidence for receptor specificity, and effect of capsule and serum. Microbiology 146: 21-30 [Abstract] [Full Text]  
• Winram, S. B., Jonas, M., Chi, E., Rubens, C. E. (1998). Characterization of Group B Streptococcal Invasion of Human Chorion and Amnion Epithelial Cells In Vitro. Infect. Immun. 66: 4932-4941 [Abstract] [Full Text]  
• Tsai, P.-J., Kuo, C.-F., Lin, K.-Y., Lin, Y.-S., Lei, H.-Y., Chen, F.-F., Wang, J.-R., Wu, J.-J. (1998). Effect of Group A Streptococcal Cysteine Protease on Invasion of Epithelial Cells. Infect. Immun. 66: 1460-1466 [Abstract] [Full Text]  
• Fluckiger, U., Jones, K. F., Fischetti, V. A. (1998). Immunoglobulins to Group A Streptococcal Surface Molecules Decrease Adherence to and Invasion of Human Pharyngeal Cells. Infect. Immun. 66: 974-979 [Abstract] [Full Text]  
• Adamou, J. E., Wizemann, T. M., Barren, P., Langermann, S. (1998). Adherence of Streptococcus pneumoniae to Human Bronchial Epithelial Cells (BEAS-2B). Infect. Immun. 66: 820-822 [Abstract] [Full Text]  
• Malin, G., Paoletti, L. C. (2001). Use of a dynamic in vitro attachment and invasion system (DIVAS) to determine influence of growth rate on invasion of respiratory epithelial cells by group B Streptococcus. Proc. Natl. Acad. Sci. USA 98: 13335-13340 [Abstract] [Full Text]