This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Henricks, P A Articles by Nijkamp, F P Search for Related Content PubMed PubMed Citation Articles by Henricks, P A Articles by Nijkamp, F P

 Previous Article  |  Next Article 

Infect Immun. 1992 August; 60(8): 3238-3243

Pasteurella haemolytica leukotoxin enhances production of leukotriene B4 and 5-hydroxyeicosatetraenoic acid by bovine polymorphonuclear leukocytes.

Department of Pharmacology, Faculty of Pharmacy, University of Utrecht, The Netherlands.

ABSTRACT

The influence of the leukotoxin of Pasteurella haemolytica on the generation of arachidonic acid metabolites by bovine polymorphonuclear leukocytes (PMNs) was investigated. PMNs released 5-, 12-, and 15-hydroxyeicosatetraenoic acids (5-, 12-, and 15-HETE) and leukotriene B4 (LTB4) upon stimulation with arachidonic acid. The leukotoxin preparations dose dependently enhanced the release of the 5-lipoxygenase products 5-HETE and LTB4 in arachidonic acid-stimulated PMNs, whereas the release of 12- and 15-HETE was not affected. The enhanced release of LTB4 and 5-HETE was not due to a decreased cellular retention of the 5-lipoxygenase products. In addition, leukotoxin preparations by themselves were also able to induce LTB4 and 5-HETE production in the absence of exogenous arachidonic acid. Generation of 5-lipoxygenase products by PMNs stimulated by leukotoxin may represent an important cellular event that occurs during infections with P. haemolytica.

This article has been cited by other articles:

• Leite, F., O'Brien, S., Sylte, M. J., Page, T., Atapattu, D., Czuprynski, C. J. (2002). Inflammatory Cytokines Enhance the Interaction of Mannheimia haemolytica Leukotoxin with Bovine Peripheral Blood Neutrophils In Vitro. Infect. Immun. 70: 4336-4343 [Abstract] [Full Text]  
• Leite, F., Brown, J. F., Sylte, M. J., Briggs, R. E., Czuprynski, C. J. (2000). Recombinant Bovine Interleukin-1beta Amplifies the Effects of Partially Purified Pasteurella haemolytica Leukotoxin on Bovine Neutrophils in a beta 2-Integrin-Dependent Manner. Infect. Immun. 68: 5581-5586 [Abstract] [Full Text]  
• Chin, A. C., Lee, W. D., Murrin, K. A., Morck, D. W., Merrill, J. K., Dick, P., Buret, A. G. (2000). Tilmicosin Induces Apoptosis in Bovine Peripheral Neutrophils in the Presence or in the Absence of Pasteurella haemolytica and Promotes Neutrophil Phagocytosis by Macrophages. Antimicrob. Agents Chemother. 44: 2465-2470 [Abstract] [Full Text]  
• Hsuan, S. L., Kannan, M. S., Jeyaseelan, S., Prakash, Y. S., Sieck, G. C., Maheswaran, S. K. (1998). Pasteurella haemolytica A1-Derived Leukotoxin and Endotoxin Induce Intracellular Calcium Elevation in Bovine Alveolar Macrophages by Different Signaling Pathways. Infect. Immun. 66: 2836-2844 [Abstract] [Full Text]  
• Stanley, P., Koronakis, V., Hughes, C. (1998). Acylation of Escherichia coli Hemolysin: A Unique Protein Lipidation Mechanism Underlying Toxin Function. Microbiol. Mol. Biol. Rev. 62: 309-333 [Abstract] [Full Text]  
• Wang, Z., Clarke, C., Clinkenbeard, K. (1998). Pasteurella haemolytica Leukotoxin-Induced Increase in Phospholipase A2 Activity in Bovine Neutrophils. Infect. Immun. 66: 1885-1890 [Abstract] [Full Text]