This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gerlach, G F Articles by Willson, P J Search for Related Content PubMed PubMed Citation Articles by Gerlach, G F Articles by Willson, P J

 Previous Article  |  Next Article 

Infect Immun. 1992 August; 60(8): 3253-3261

Characterization of two genes encoding distinct transferrin-binding proteins in different Actinobacillus pleuropneumoniae isolates.

Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada.

ABSTRACT

The gene encoding the Actinobacillus pleuropneumoniae serotype 1 transferrin-binding protein (tfbA) was cloned, and the carboxy-terminal 70% of the protein was expressed as an aggregate protein in Escherichia coli. The nucleotide sequences of the tfbA genes from A. pleuropneumoniae serotypes 7 (G.-F. Gerlach, C. Anderson, A. A. Potter, S. Klashinsky, and P. J. Willson, Infect. Immun. 60:892-898, 1992) and 1 were determined, and a comparison revealed that they had 65% sequence identity. The deduced amino acid sequences showed a sequence agreement of 55%, and both proteins possessed a lipoprotein-like signal sequence. The serotype 1 TfbA protein had a predicted molecular mass of 65 kDa, compared with 60 kDa for the serotype 7 TfbA protein, and both proteins were immunologically distinct as assessed in a competitive enzyme-linked immunosorbent assay. Southern hybridization and Western blot (immunoblot) analysis of the 13 A. pleuropneumoniae type strains revealed that serotypes 2, 3, 4, 8, 9, 10, and 11 encode and express a TfbA protein highly homologous to that of A. pleuropneumoniae serotype 7 whereas the TfbA proteins and the encoding genes of serotypes 6 and 12 were highly homologous to that found in A. pleuropneumoniae serotype 1. The tfbA genes of A. pleuropneumoniae serotypes 5A and 5B were recognized, under medium-stringency hybridization conditions, by the A. pleuropneumoniae serotype 1-derived tfbA probe, and the respective proteins were weakly reactive with the antibody raised against the A. pleuropneumoniae serotype 7 TfbA protein.

This article has been cited by other articles:

• Maas, A., Jacobsen, I. D., Meens, J., Gerlach, G.-F. (2006). Use of an Actinobacillus pleuropneumoniae Multiple Mutant as a Vaccine That Allows Differentiation of Vaccinated and Infected Animals. Infect. Immun. 74: 4124-4132 [Abstract] [Full Text]  
• Nielsen, K. K., Boye, M. (2005). Real-Time Quantitative Reverse Transcription-PCR Analysis of Expression Stability of Actinobacillus pleuropneumoniae Housekeeping Genes during In Vitro Growth under Iron-Depleted Conditions. Appl. Environ. Microbiol. 71: 2949-2954 [Abstract] [Full Text]  
• Oakhill, J. S., Sutton, B. J., Gorringe, A. R., Evans, R. W. (2005). Homology modelling of transferrin-binding protein A from Neisseria meningitidis. Protein Eng Des Sel 18: 221-228 [Abstract] [Full Text]  
• Baltes, N., Hennig-Pauka, I., Jacobsen, I., Gruber, A. D., Gerlach, G. F. (2003). Identification of Dimethyl Sulfoxide Reductase in Actinobacillus pleuropneumoniae and Its Role in Infection. Infect. Immun. 71: 6784-6792 [Abstract] [Full Text]  
• Mikael, L. G., Pawelek, P. D., Labrie, J., Sirois, M., Coulton, J. W., Jacques, M. (2002). Molecular cloning and characterization of the ferric hydroxamate uptake (fhu) operon in Actinobacillus pleuropneumoniae. Microbiology 148: 2869-2882 [Abstract] [Full Text]  
• Subramaniam, S., Huang, B., Loh, H., Kwang, J., Tan, H.-M., Chua, K.-L., Frey, J. (2000). Characterization of a Predominant Immunogenic Outer Membrane Protein of Riemerella anatipestifer. CVI 7: 168-174 [Abstract] [Full Text]  
• Tonpitak, W., Thiede, S., Oswald, W., Baltes, N., Gerlach, G.-F. (2000). Actinobacillus pleuropneumoniae Iron Transport: a Set of exbBD Genes Is Transcriptionally Linked to the tbpB Gene and Required for Utilization of Transferrin-Bound Iron. Infect. Immun. 68: 1164-1170 [Abstract] [Full Text]  
• Oswald, W., Konine, D. V., Rohde, J., Gerlach, G.-F. (1999). First Chromosomal Restriction Map of Actinobacillus pleuropneumoniae and Localization of Putative Virulence-Associated Genes. J. Bacteriol. 181: 4161-4169 [Abstract] [Full Text]  
• Retzer, M. D., Kabani, A., Button, L. L., Yu, R.-h., Schryvers, A. B. (1996). Production and Characterization of Chimeric Transferrins for the Determination of the Binding Domains for Bacterial Transferrin Receptors. J. Biol. Chem. 271: 1166-1173 [Abstract] [Full Text]