This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chia, J S Articles by Yang, C S Search for Related Content PubMed PubMed Citation Articles by Chia, J S Articles by Yang, C S

 Previous Article  |  Next Article 

Infect Immun. 1993 November; 61(11): 4689-4695

Inhibition of glucosyltransferase activities of Streptococcus mutans by a monoclonal antibody to a subsequence peptide.

Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Republic of China.

ABSTRACT

Preliminary analysis indicated that a 19-amino-acid peptide sequence (435 to 453 of GtfC) within a highly conserved region of the glucosyltransferases of the cariogenic streptococci might be functionally important (J.-S. Chia, S.-W. Lin, T.-Y. Hsu, J.-Y. Chen, H.-W. Kwan, and C.-S. Yang, Infect. Immun. 61:1563-1566, 1993). To obtain antipeptide monoclonal antibodies (MAbs), the 19-amino-acid peptide was conjugated to bovine serum albumin and used as an antigen in BALB/c mice. Six immunoglobulin G-secreting hybridoma clones, CJSm18-S1 to -S6, specifically reacted with this peptide and with purified GtfC and GtfD but not with bovine serum albumin in an enzyme-linked immunosorbent assay. The concentrated hybridoma supernatant of all six MAbs inhibited GtfC enzymatic activity but failed to inhibit GtfD, although GtfD contains the same peptide sequence. Further analysis of a purified immunoglobulin G2b MAb from one of the clones, CJSm18-S3, confirmed that this MAb specifically inhibited GtfC enzymatic activity for insoluble-glucan synthesis in a dose-dependent manner. CJSm18-S3, even at high concentrations, had no effect on GtfD, which synthesizes water-soluble glucan exclusively. Furthermore, the in vitro sucrose-dependent adherence of Streptococcus mutans was also inhibited by CJSm18-S3 in a dose-dependent manner. Our results indicate that the peptide containing the N-terminal conserved region of glucosyltransferases is functionally important for both enzymatic activity and bacterial adherence.

This article has been cited by other articles:

• Culshaw, S., LaRosa, K., Tolani, H., Han, X., Eastcott, J. W., Smith, D. J., Taubman, M. A. (2007). Immunogenic and Protective Potential of Mutans Streptococcal Glucosyltransferase Peptide Constructs Selected by Major Histocompatibility Complex Class II Allele Binding. Infect. Immun. 75: 915-923 [Abstract] [Full Text]  
• van Hijum, S. A. F. T., Kralj, S., Ozimek, L. K., Dijkhuizen, L., van Geel-Schutten, I. G. H. (2006). Structure-Function Relationships of Glucansucrase and Fructansucrase Enzymes from Lactic Acid Bacteria. Microbiol. Mol. Biol. Rev. 70: 157-176 [Abstract] [Full Text]  
• Yeh, C.-Y., Chen, J.-Y., Chia, J.-S. (2006). Glucosyltransferases of Viridans Group Streptococci Modulate Interleukin-6 and Adhesion Molecule Expression in Endothelial Cells and Augment Monocytic Cell Adherence. Infect. Immun. 74: 1273-1283 [Abstract] [Full Text]  
• Guo, J.H., Jia, R., Fan, M.W., Bian, Z., Chen, Z., Peng, B. (2004). Construction and Immunogenic Characterization of a Fusion Anti-caries DNA Vaccine against PAc and Glucosyltransferase I of Streptococcus mutans. J. Dent. Res. 83: 266-270 [Abstract] [Full Text]  
• Smith, D.J. (2002). DENTAL CARIES VACCINES: PROSPECTS AND CONCERNS. Crit. Rev. Oral Biol. Med. 13: 335-349 [Abstract] [Full Text]  
• Jespersgaard, C., Zhang, P., Hajishengallis, G., Russell, M. W., Michalek, S. M. (2001). Effect of Attenuated Salmonella enterica Serovar Typhimurium Expressing a Streptococcus mutans Antigen on Secondary Responses to the Cloned Protein. Infect. Immun. 69: 6604-6611 [Abstract] [Full Text]  
• Smith, D. J., King, W. F., Barnes, L. A., Trantolo, D., Wise, D. L., Taubman, M. A. (2001). Facilitated Intranasal Induction of Mucosal and Systemic Immunity to Mutans Streptococcal Glucosyltransferase Peptide Vaccines. Infect. Immun. 69: 4767-4773 [Abstract] [Full Text]  
• Chia, J.-S., You, C.-M., Hu, C.-Y., Chiang, B.-L., Chen, J.-Y. (2001). Human T-Cell Responses to the Glucosyltransferases of Streptococcus mutans. CVI 8: 441-445 [Abstract] [Full Text]  
• Chia, J.-S., Yeh, C.-Y., Chen, J.-Y. (2000). Identification of a Fibronectin Binding Protein from Streptococcus mutans. Infect. Immun. 68: 1864-1870 [Abstract] [Full Text]  
• Jespersgaard, C., Hajishengallis, G., Huang, Y., Russell, M. W., Smith, D. J., Michalek, S. M. (1999). Protective Immunity against Streptococcus mutans Infection in Mice after Intranasal Immunization with the Glucan-Binding Region of S. mutans Glucosyltransferase. Infect. Immun. 67: 6543-6549 [Abstract] [Full Text]  
• Smith, D. J., Heschel, R. L., King, W. F., Taubman, M. A. (1999). Antibody to Glucosyltransferase Induced by Synthetic Peptides Associated with Catalytic Regions of alpha -Amylases. Infect. Immun. 67: 2638-2642 [Abstract] [Full Text]  
• Jespersgaard, C., Hajishengallis, G., Greenway, T. E., Smith, D. J., Russell, M. W., Michalek, S. M. (1999). Functional and Immunogenic Characterization of Two Cloned Regions of Streptococcus mutans Glucosyltransferase I. Infect. Immun. 67: 810-816 [Abstract] [Full Text]