This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wessels, M R Articles by Kasper, D L Search for Related Content PubMed PubMed Citation Articles by Wessels, M R Articles by Kasper, D L

 Previous Article  |  Next Article 

Infect Immun. 1993 November; 61(11): 4760-4766

Stimulation of protective antibodies against type Ia and Ib group B streptococci by a type Ia polysaccharide-tetanus toxoid conjugate vaccine.

Division of Infectious Diseases, Beth Israel Hospital, Boston, Massachusetts.

ABSTRACT

Antisera elicited by type Ia group B streptococci (GBS) contain antibodies that react with both type Ia and type Ib strains. Previous studies suggested that antibodies elicited by type Ia organisms recognized a carbohydrate antigen or epitope common to Ia and Ib strains. We now report the synthesis and immunogenicity testing of a type Ia polysaccharide-tetanus toxoid (Ia-TT) conjugate vaccine. Ia-TT elicited type Ia polysaccharide-specific immunoglobulin G antibodies in all three of the rabbits inoculated. In competitive enzyme-linked immunosorbent assay, these antibodies reacted with high affinity to type Ia polysaccharide and with lower affinity to the structurally related GBS type Ib polysaccharide. Despite the lower binding affinity of the Ia-TT-induced antibodies for the type Ib polysaccharide, Ia-TT antiserum opsonized not only type Ia GBS but also type Ib GBS for killing by human blood leukocytes. Ia-TT antiserum was also evaluated in a mouse model designed to test the efficacy of maternal antibodies in protecting neonates against GBS infection. Pups born to dams that had received Ia-TT antiserum were protected against lethal challenge with either type Ia or Ib GBS. These studies using a polysaccharide-protein conjugate as an immunogen support the view that the carbohydrate immunodeterminant recognized on Ib strains by Ia antisera is a common epitope contained within the structurally related Ia and Ib capsular polysaccharides. Although antibodies elicited by Ia-TT had protective activity against both Ia and Ib strains, these antibodies reacted with lower affinity to Ib than to Ia polysaccharide.

This article has been cited by other articles:

• Jiang, S.-M., Ishmael, N., Hotopp, J. D., Puliti, M., Tissi, L., Kumar, N., Cieslewicz, M. J., Tettelin, H., Wessels, M. R. (2008). Variation in the Group B Streptococcus CsrRS Regulon and Effects on Pathogenicity. J. Bacteriol. 190: 1956-1965 [Abstract] [Full Text]  
• Tettelin, H., Masignani, V., Cieslewicz, M. J., Donati, C., Medini, D., Ward, N. L., Angiuoli, S. V., Crabtree, J., Jones, A. L., Durkin, A. S., DeBoy, R. T., Davidsen, T. M., Mora, M., Scarselli, M., Margarit y Ros, I., Peterson, J. D., Hauser, C. R., Sundaram, J. P., Nelson, W. C., Madupu, R., Brinkac, L. M., Dodson, R. J., Rosovitz, M. J., Sullivan, S. A., Daugherty, S. C., Haft, D. H., Selengut, J., Gwinn, M. L., Zhou, L., Zafar, N., Khouri, H., Radune, D., Dimitrov, G., Watkins, K., O'Connor, K. J. B., Smith, S., Utterback, T. R., White, O., Rubens, C. E., Grandi, G., Madoff, L. C., Kasper, D. L., Telford, J. L., Wessels, M. R., Rappuoli, R., Fraser, C. M. (2005). Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: Implications for the microbial "pan-genome". Proc. Natl. Acad. Sci. USA 102: 13950-13955 [Abstract] [Full Text]  
• Jiang, S.-M., Cieslewicz, M. J., Kasper, D. L., Wessels, M. R. (2005). Regulation of Virulence by a Two-Component System in Group B Streptococcus. J. Bacteriol. 187: 1105-1113 [Abstract] [Full Text]  
• Martin, D., Rioux, S., Gagnon, E., Boyer, M., Hamel, J., Charland, N., Brodeur, B. R. (2002). Protection from Group B Streptococcal Infection in Neonatal Mice by Maternal Immunization with Recombinant Sip Protein. Infect. Immun. 70: 4897-4901 [Abstract] [Full Text]  
• Rioux, S., Martin, D., Ackermann, H.-W., Dumont, J., Hamel, J., Brodeur, B. R. (2001). Localization of Surface Immunogenic Protein on Group B Streptococcus. Infect. Immun. 69: 5162-5165 [Abstract] [Full Text]  
• Brodeur, B. R., Boyer, M., Charlebois, I., Hamel, J., Couture, F., Rioux, C. R., Martin, D. (2000). Identification of Group B Streptococcal Sip Protein, Which Elicits Cross-Protective Immunity. Infect. Immun. 68: 5610-5618 [Abstract] [Full Text]  
• Paoletti, L. J., Bradford, J., Paoletti, L. C. (1999). A Serotype VIII Strain among Colonizing Group B Streptococcal Isolates in Boston, Massachusetts. J. Clin. Microbiol. 37: 3759-3760 [Abstract] [Full Text]  
• Ross, R. A., Madoff, L. C., Paoletti, L. C. (1999). Regulation of Cell Component Production by Growth Rate in the Group B Streptococcus. J. Bacteriol. 181: 5389-5394 [Abstract] [Full Text]  
• Wessels, M. R., Paoletti, L. C., Guttormsen, H.-K., Michon, F., D'Ambra, A. J., Kasper, D. L. (1998). Structural Properties of Group B Streptococcal Type III Polysaccharide Conjugate Vaccines That Influence Immunogenicity and Efficacy. Infect. Immun. 66: 2186-2192 [Abstract] [Full Text]  
• Li, J., Kasper, D. L., Ausubel, F. M., Rosner, B., Michel, J. L. (1997). Inactivation of the alpha  C protein antigen gene, bca, by a novel shuttle/suicide vector results in attenuation of virulence and immunity in group B Streptococcus. Proc. Natl. Acad. Sci. USA 94: 13251-13256 [Abstract] [Full Text]  
• Kogan, G., Uhrín, D., Brisson, J.-R., Paoletti, L. C., Blodgett, A. E., Kasper, D. L., Jennings, H. J. (1996). Structural and Immunochemical Characterization of the Type VIII Group B Streptococcus Capsular Polysaccharide. J. Biol. Chem. 271: 8786-8790 [Abstract] [Full Text]  
• Cieslewicz, M. J., Kasper, D. L., Wang, Y., Wessels, M. R. (2001). Functional Analysis in Type Ia Group B Streptococcus of a Cluster of Genes Involved in Extracellular Polysaccharide Production by Diverse Species of Streptococci. J. Biol. Chem. 276: 139-146 [Abstract] [Full Text]