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Infect Immun. 1993 August; 61(8): 3313-3317

Characterization of epitopes recognized by Candida factor 1 and 9 antisera by use of Saccharomyces cerevisiae mnn mutants.

H Ataoglu, J Zueco and R Sentandreu

Departamento de Microbiología, Facultat de Farmàcia, Universitat de València, Burjassot, Spain.

ABSTRACT

The use of Saccharomyces cerevisiae mnn mutants has facilitated the study of the epitopes recognized by antisera against several antigenic factors of the genus Candida (Candida Check; Iatron Laboratories, Tokyo, Japan). We have taken advantage of the very well characterized structure of the mannans of the different mnn mutants to compare their reactivities with the factor antisera used in the identification of different species of the genus Candida. The results of this study provide evidence that one of the antigenic determinants recognized by factor 1 antisera is the O-linked mannose chains of the cell wall mannoproteins, while that recognized by factor 9 antiserum is the alpha 1-6-linked mannose backbone of the outer chain of the N-linked oligosaccharide.

Infect Immun. 1993 August; 61(8): 3313-3317




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