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Infection and Immunity, September 1994, p. 3901-3906, Vol. 62, No. 9
0019-9567/1994/$04.00+0     DOI:

research-article

Differential flagellin expression in a flaA flaB+ mutant of Campylobacter jejuni.

T M Wassenaar, N M Bleumink-Pluym, D G Newell, P J Nuijten, and B A van der Zeijst

Department of Bacteriology, School of Veterinary Medicine, University of Utrecht, The Netherlands.

ABSTRACT

Campylobacter jejuni 81116 has two genes coding for flagellin, flaA and flaB. Fully motile wild-type C. jejuni bacteria express the flaA gene, with no flaB message being detected. A nonmotile flaA flaB+ mutant, R1, produced detectable levels of flagellin B which was incorporated into truncated flagella. After R1 had invaded INT-407 cells, a variant with increased motility, R1-V2, was isolated. R1-V2 produced full-length flagella and an increased amount of flagellin B. Transcriptional analysis showed that R1-V2 contained more flaB mRNA than its parental strain, R1. The flaB gene promoter sequence and primer extension experiments confirmed that transcription of the flaB gene is initiated from a sigma 54 promoter. Neither the promoter sequence nor the coding sequence of flaB had changed in R1-V2. In contrast to R1, R1-V2 no longer produced (truncated) flaA mRNA. The sigma 28 flaA promoter sequence was not changed in R1-V2. We propose that expression of the two flagellin genes in C. jejuni 81116 is regulated at the transcriptional level, in such a way that predominantly one gene at a time is transcribed. We compared the levels of invasiveness of the wild-type strain, R1, and R1-V2 for INT-407 cells. The shift in expression from flaA to flaB occurred not only during invasion assays but also under different conditions in the absence of eukaryotic cells.

Infection and Immunity, September 1994, p. 3901-3906, Vol. 62, No. 9
0019-9567/1994/$04.00+0     DOI:




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