This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vila, L. M. Articles by Holoshitz, J. Search for Related Content PubMed PubMed Citation Articles by Vila, L. M. Articles by Holoshitz, J.

 Previous Article  |  Next Article 

Infect. Immun., 04 1995, 1211-1217, Vol 63, No. 4
Copyright © 1995, American Society for Microbiology

Expansion of mycobacterium-reactive gamma delta T cells by a subset of memory helper T cells

LM Vila, HM Haftel, HS Park, MS Lin, NC Romzek, SM Hanash and J Holoshitz
University of Michigan Specialized Center of Research in Rheumatoid Arthritis, Department of Internal Medicine, Ann Arbor.

Human gamma delta T cells expressing the V gamma 9/V delta 2 T-cell receptor have been previously found to proliferate in response to certain microorganisms and to expand throughout life, presumably because of extrathymic activation by foreign antigens. In vitro expansion of V gamma 9/V delta 2 cells by mycobacteria has been previously shown to be dependent on accessory cells. In order to gain an insight into the mechanisms involved in the expansion of these cells, we have undertaken to identify the peripheral blood subset of cells on which proliferation of V gamma 9/V delta 2 cells in response to mycobacteria is dependent. Contrary to their role in antigen presentation to alpha beta T cells, professional antigen-presenting cells, such as monocytes, B cells, and dendritic cells, were unable to provide the cellular support for the expansion of V gamma 9/V delta 2 cells. Selective depletion of T-cell subsets, as well as the use of highly purified T-cell populations, indicated that the only subset of peripheral blood cells that could expand V gamma 9/V delta 2 cells were CD4+ CD45RO+ CD7- alpha beta T cells. These cells underwent distinct intracellular signaling events after stimulation with the mycobacterial antigen. Expansion of V gamma 9/V delta 2 cells by alpha beta T cells was dependent on cell-cell contact. This is the first evidence that a small subset of the memory helper T-cell population is exclusively responsible for the peripheral expansion of V gamma 9/V delta 2 cells. These data illustrate a unique aspect of antigen recognition by gamma delta T cells and provide new means to study their immune defense role.

This article has been cited by other articles:

• Penido, C., Vieira-de-Abreu, A., Bozza, M. T., Castro-Faria-Neto, H. C., Bozza, P. T. (2003). Role of Monocyte Chemotactic Protein-1/CC Chemokine Ligand 2 on {gamma}{delta} T Lymphocyte Trafficking during Inflammation Induced by Lipopolysaccharide or Mycobacterium bovis Bacille Calmette-Guerin. J. Immunol. 171: 6788-6794 [Abstract] [Full Text]  
• Van Rhijn, I., Van den Berg, L. H., Ang, C. W., Admiraal, J., Logtenberg, T. (2003). Expansion of human {gamma}{delta} T cells after in vitro stimulation with Campylobacter jejuni. Int Immunol 15: 373-382 [Abstract] [Full Text]  
• Vincent, M. S., Roessner, K., Sellati, T., Huston, C. D., Sigal, L. H., Behar, S. M., Radolf, J. D., Budd, R. C. (1998). Lyme Arthritis Synovial {gamma}{delta} T Cells Respond to Borrelia burgdorferi Lipoproteins and Lipidated Hexapeptides. J. Immunol. 161: 5762-5771 [Abstract] [Full Text]  
• Hoft, D. F., Brown, R. M., Roodman, S. T. (1998). Bacille Calmette-Guerin Vaccination Enhances Human {gamma}{delta} T Cell Responsiveness to Mycobacteria Suggestive of a Memory-Like Phenotype. J. Immunol. 161: 1045-1054 [Abstract] [Full Text]