This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Darveau, R. P. Articles by Aruffo, A. Search for Related Content PubMed PubMed Citation Articles by Darveau, R. P. Articles by Aruffo, A.

 Previous Article  |  Next Article 

Infect. Immun., Apr 1995, 1311-1317, Vol 63, No. 4
Copyright © 1995, American Society for Microbiology

Ability of bacteria associated with chronic inflammatory disease to stimulate E-selectin expression and promote neutrophil adhesion

RP Darveau, MD Cunningham, T Bailey, C Seachord, K Ratcliffe, B Bainbridge, M Dietsch, RC Page and A Aruffo
Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, WA 98121.

Porphyromonas gingivalis, Pseudomonas aeruginosa, and Helicobacter pylori have been shown to be associated with adult periodontal disease, chronic lung infections, and peptic ulcers, respectively. The ability of these bacteria to stimulate E-selectin expression and promote neutrophil adhesion, two components necessary for the recruitment of leukocytes in response to infection, was investigated. Little or no stimulation of E-selectin expression was observed with either P. gingivalis or H. pylori when whole cells, lipopolysaccharide (LPS), or cell wall preparations added to human umbilical cord vein endothelial cells were examined. P. aeruginosa was able to induce E-selectin to near-maximal levels; however, it required approximately 100 to 1,000 times more whole cells or LPS than that required by E. coli. Neutrophil- binding assays revealed that LPS and cell wall preparations obtained from these bacteria did not promote endothelial cell adhesiveness by E- selectin-independent mechanisms. In addition, P. gingivalis LPS blocked E-selectin expression by LPS obtained from other bacteria. We propose that lack of E-selectin stimulation and the inability to promote endothelial cell adhesiveness are two additional indications of low biologically reactive LPS. We suggest that this property of LPS may contribute to host tissue colonization. In addition, the ability of P. gingivalis to inhibit E-selectin expression may represent a new virulence factor for this organism.

This article has been cited by other articles:

• Bainbridge, B. W., Karimi-Naser, L., Reife, R., Blethen, F., Ernst, R. K., Darveau, R. P. (2008). Acyl Chain Specificity of the Acyltransferases LpxA and LpxD and Substrate Availability Contribute to Lipid A Fatty Acid Heterogeneity in Porphyromonas gingivalis. J. Bacteriol. 190: 4549-4558 [Abstract] [Full Text]  
• Erridge, C., Spickett, C. M., Webb, D. J. (2007). Non-enterobacterial endotoxins stimulate human coronary artery but not venous endothelial cell activation via Toll-like receptor 2. Cardiovasc Res 73: 181-189 [Abstract] [Full Text]  
• d'Empaire, G., Baer, M. T., Gibson, F. C. III (2006). The K1 Serotype Capsular Polysaccharide of Porphyromonas gingivalis Elicits Chemokine Production from Murine Macrophages That Facilitates Cell Migration. Infect. Immun. 74: 6236-6243 [Abstract] [Full Text]  
• Yun, P. L. W., DeCarlo, A. A., Hunter, N. (2006). Gingipains of Porphyromonas gingivalis Modulate Leukocyte Adhesion Molecule Expression Induced in Human Endothelial Cells by Ligation of CD99. Infect. Immun. 74: 1661-1672 [Abstract] [Full Text]  
• Ernst, R. K., Adams, K. N., Moskowitz, S. M., Kraig, G. M., Kawasaki, K., Stead, C. M., Trent, M. S., Miller, S. I. (2006). The Pseudomonas aeruginosa Lipid A Deacylase: Selection for Expression and Loss within the Cystic Fibrosis Airway. J. Bacteriol. 188: 191-201 [Abstract] [Full Text]  
• Coats, S. R., Pham, T.-T. T., Bainbridge, B. W., Reife, R. A., Darveau, R. P. (2005). MD-2 Mediates the Ability of Tetra-Acylated and Penta-Acylated Lipopolysaccharides to Antagonize Escherichia coli Lipopolysaccharide at the TLR4 Signaling Complex. J. Immunol. 175: 4490-4498 [Abstract] [Full Text]  
• Dixon, D.R., Darveau, R.P. (2005). Lipopolysaccharide Heterogeneity: Innate Host Responses to Bacterial Modification of Lipid A Structure. J. Dent. Res. 84: 584-595 [Abstract] [Full Text]  
• Zhou, Q., Desta, T., Fenton, M., Graves, D. T., Amar, S. (2005). Cytokine Profiling of Macrophages Exposed to Porphyromonas gingivalis, Its Lipopolysaccharide, or Its FimA Protein. Infect. Immun. 73: 935-943 [Abstract] [Full Text]  
• Walter, C., Zahlten, J., Schmeck, B., Schaudinn, C., Hippenstiel, S., Frisch, E., Hocke, A. C., Pischon, N., Kuramitsu, H. K., Bernimoulin, J.-P., Suttorp, N., Krull, M. (2004). Porphyromonas gingivalis Strain-Dependent Activation of Human Endothelial Cells. Infect. Immun. 72: 5910-5918 [Abstract] [Full Text]  
• Darveau, R. P., Pham, T.-T. T., Lemley, K., Reife, R. A., Bainbridge, B. W., Coats, S. R., Howald, W. N., Way, S. S., Hajjar, A. M. (2004). Porphyromonas gingivalis Lipopolysaccharide Contains Multiple Lipid A Species That Functionally Interact with Both Toll-Like Receptors 2 and 4. Infect. Immun. 72: 5041-5051 [Abstract] [Full Text]  
• Cohen, N., Morisset, J., Emilie, D. (2004). Induction of Tolerance by Porphyromonas gingivalis on APCs: a Mechanism Implicated in Periodontal Infection. J. Dent. Res. 83: 429-433 [Abstract] [Full Text]  
• Coats, S. R., Reife, R. A., Bainbridge, B. W., Pham, T.-T. T., Darveau, R. P. (2003). Porphyromonas gingivalis Lipopolysaccharide Antagonizes Escherichia coli Lipopolysaccharide at Toll-Like Receptor 4 in Human Endothelial Cells. Infect. Immun. 71: 6799-6807 [Abstract] [Full Text]  
• Ellerbroek, P. M., Hoepelman, A. I. M., Wolbers, F., Zwaginga, J. J., Coenjaerts, F. E. J. (2002). Cryptococcal Glucuronoxylomannan Inhibits Adhesion of Neutrophils to Stimulated Endothelium In Vitro by Affecting Both Neutrophils and Endothelial Cells. Infect. Immun. 70: 4762-4771 [Abstract] [Full Text]  
• Darveau, R. P., Arbabi, S., Garcia, I., Bainbridge, B., Maier, R. V. (2002). Porphyromonas gingivalis Lipopolysaccharide Is Both Agonist and Antagonist for p38 Mitogen-Activated Protein Kinase Activation. Infect. Immun. 70: 1867-1873 [Abstract] [Full Text]  
• Yoshimura, A., Kaneko, T., Kato, Y., Golenbock, D. T., Hara, Y. (2002). Lipopolysaccharides from Periodontopathic Bacteria Porphyromonas gingivalis and Capnocytophaga ochracea Are Antagonists for Human Toll-Like Receptor 4. Infect. Immun. 70: 218-225 [Abstract] [Full Text]  
• Khlgatian, M., Nassar, H., Chou, H.-H., Gibson, F. C. III, Genco, C. A. (2002). Fimbria-Dependent Activation of Cell Adhesion Molecule Expression in Porphyromonas gingivalis-Infected Endothelial Cells. Infect. Immun. 70: 257-267 [Abstract] [Full Text]  
• Gemmell, E., Yamazaki, K., Seymour, G.J. (2002). DESTRUCTIVE PERIODONTITIS LESIONS ARE DETERMINED BY THE NATURE OF THE LYMPHOCYTIC RESPONSE. Crit. Rev. Oral Biol. Med. 13: 17-34 [Abstract] [Full Text]  
• Gunn, J. S., Ernst, R. K., McCoy, A. J., Miller, S. I. (2000). Constitutive Mutations of the Salmonella enterica Serovar Typhimurium Transcriptional Virulence Regulator phoP. Infect. Immun. 68: 3758-3762 [Abstract] [Full Text]  
• Cunningham, M. D., Shapiro, R. A., Seachord, C., Ratcliffe, K., Cassiano, L., Darveau, R. P. (2000). CD14 Employs Hydrophilic Regions to ""Capture"" Lipopolysaccharides. J. Immunol. 164: 3255-3263 [Abstract] [Full Text]  
• Lush, C. W., Cepinskas, G., Kvietys, P. R. (2000). LPS tolerance in human endothelial cells: reduced PMN adhesion, E-selectin expression, and NF-kappa B mobilization. Am. J. Physiol. Heart Circ. Physiol. 278: H853-H861 [Abstract] [Full Text]  
• Giese, M. J., Shum, D. C., Rayner, S. A., Mondino, B. J., Berliner, J. A. (2000). Adhesion Molecule Expression in a Rat Model of Staphylococcus aureus Endophthalmitis. IOVS 41: 145-153 [Abstract] [Full Text]  
• Somerville, J. E. Jr., Cassiano, L., Darveau, R. P. (1999). Escherichia coli msbB Gene as a Virulence Factor and a Therapeutic Target. Infect. Immun. 67: 6583-6590 [Abstract] [Full Text]  
• Ernst, R. K., Yi, E. C., Guo, L., Lim, K. B., Burns, J. L., Hackett, M., Miller, S. I. (1999). Specific Lipopolysaccharide Found in Cystic Fibrosis Airway Pseudomonas aeruginosa. Science 286: 1561-1565 [Abstract] [Full Text]  
• Neviere, R. R., Cepinskas, G., Madorin, W. S., Hoque, N., Karmazyn, M., Sibbald, W. J., Kvietys, P. R. (1999). LPS pretreatment ameliorates peritonitis-induced myocardial inflammation and dysfunction: role of myocytes. Am. J. Physiol. Heart Circ. Physiol. 277: H885-H892 [Abstract] [Full Text]  
• Percival, R. S., Marsh, P. D., Devine, D. A., Rangarajan, M., Aduse-Opoku, J., Shepherd, P., Curtis, M. A. (1999). Effect of Temperature on Growth, Hemagglutination, and Protease Activity of Porphyromonas gingivalis. Infect. Immun. 67: 1917-1921 [Abstract] [Full Text]  
• Lamont, R. J., Jenkinson, H. F. (1998). Life Below the Gum Line: Pathogenic Mechanisms of Porphyromonas gingivalis. Microbiol. Mol. Biol. Rev. 62: 1244-1263 [Abstract] [Full Text]  
• Deshpande, R. G., Khan, M. B., Attardo Genco, C. (1998). Invasion of Aortic and Heart Endothelial Cells by Porphyromonas gingivalis. Infect. Immun. 66: 5337-5343 [Abstract] [Full Text]  
• Bliss, C. M. Jr., Golenbock, D. T., Keates, S., Linevsky, J. K., Kelly, C. P. (1998). Helicobacter pylori Lipopolysaccharide Binds to CD14 and Stimulates Release of Interleukin-8, Epithelial Neutrophil-Activating Peptide 78, and Monocyte Chemotactic Protein 1 by Human Monocytes. Infect. Immun. 66: 5357-5363 [Abstract] [Full Text]  
• Shapira, L., Champagne, C., Van Dyke, T. E., Amar, S. (1998). Strain-Dependent Activation of Monocytes and Inflammatory Macrophages by Lipopolysaccharide of Porphyromonas gingivalis. Infect. Immun. 66: 2736-2742 [Abstract] [Full Text]  
• Darveau, R. P., Belton, C. M., Reife, R. A., Lamont, R. J. (1998). Local Chemokine Paralysis, a Novel Pathogenic Mechanism for Porphyromonas gingivalis. Infect. Immun. 66: 1660-1665 [Abstract] [Full Text]  
• Guo, L., Lim, K. B., Gunn, J. S., Bainbridge, B., Darveau, R. P., Hackett, M., Miller, S. I. (1997). Regulation of Lipid A Modifications by Salmonella typhimurium Virulence Genes phoP-phoQ. Science 276: 250-253 [Abstract] [Full Text]