This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Marsh, J. W. Articles by Taylor, R. K. Search for Related Content PubMed PubMed Citation Articles by Marsh, J. W. Articles by Taylor, R. K.

 Previous Article  |  Next Article 

Infect. Immun., 02 1996, 460-465, Vol 64, No. 2
Copyright © 1996, American Society for Microbiology

Physical linkage of the Vibrio cholerae mannose-sensitive hemagglutinin secretory and structural subunit gene loci: identification of the mshG coding sequence

JW Marsh, D Sun and RK Taylor
Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.

Vibrio cholerae O1 expresses a variety of cell surface factors which mediate bacterial adherence and colonization at the intestinal epithelium. The mannose-sensitive hemagglutinin (MSHA), a type IV pilus, is a potential attachment factor of the V. cholerae El Tor biotype. We describe a TnphoA mutant that is defective in its ability to hemagglutinate mouse erythrocytes. The TnphoA insertion maps to a recently identified genetic locus that encodes products that are predicted to be essential for assembly and export of the MSHA pilus. Insertional disruption at this locus in a mshA-phoA reporter strain provides evidence for a role of this locus in the latter stages of pilus assembly and/or export. These constructs have provided physical markers by which we have established close physical linkage of this secretion locus to a set of genes that includes the mshA structural gene. Sequence analysis of the intervening region between these two loci has revealed the presence of an open reading frame with homology to pilus biogenesis genes of several gram-negative bacteria. This genetic organization suggests an entire operon encoding the MSHA pilus and the components necessary for its assembly and secretion to the bacterial cell surface. The nomenclature of the MSHA structural and secretory locus has been redefined accordingly.

This article has been cited by other articles:

• Talyzina, N. M., Ingvarsson, P. K., Zhu, J., Wai, S. N., Andersson, A. (2009). Molecular Diversification in the Quorum-Sensing System of Vibrio cholerae: Role of Natural Selection in the Emergence of Pandemic Strains. Appl. Environ. Microbiol. 75: 3808-3812 [Abstract] [Full Text]  
• Fong, J. C. N., Yildiz, F. H. (2008). Interplay between Cyclic AMP-Cyclic AMP Receptor Protein and Cyclic di-GMP Signaling in Vibrio cholerae Biofilm Formation. J. Bacteriol. 190: 6646-6659 [Abstract] [Full Text]  
• Stabb, E. V., Ruby, E. G. (2003). Contribution of pilA to Competitive Colonization of the Squid Euprymna scolopes by Vibrio fischeri. Appl. Environ. Microbiol. 69: 820-826 [Abstract] [Full Text]  
• Chiavelli, D. A., Marsh, J. W., Taylor, R. K. (2001). The Mannose-Sensitive Hemagglutinin of Vibrio cholerae Promotes Adherence to Zooplankton. Appl. Environ. Microbiol. 67: 3220-3225 [Abstract] [Full Text]  
• Sandkvist, M. (2001). Type II Secretion and Pathogenesis. Infect. Immun. 69: 3523-3535 [Full Text]  
• Marsh, J. W., Taylor, R. K. (1999). Genetic and Transcriptional Analyses of the Vibrio cholerae Mannose-Sensitive Hemagglutinin Type 4 Pilus Gene Locus. J. Bacteriol. 181: 1110-1117 [Abstract] [Full Text]  
• Trucksis, M., Michalski, J., Deng, Y. K., Kaper, J. B. (1998). The Vibrio cholerae genome contains two unique circular chromosomes. Proc. Natl. Acad. Sci. USA 95: 14464-14469 [Abstract] [Full Text]  
• Jouravleva, E. A., McDonald, G. A., Marsh, J. W., Taylor, R. K., Boesman-Finkelstein, M., Finkelstein, R. A. (1998). The Vibrio cholerae Mannose-Sensitive Hemagglutinin Is the Receptor for a Filamentous Bacteriophage from V. cholerae O139. Infect. Immun. 66: 2535-2539 [Abstract] [Full Text]