This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wiktor-Jedrzejczak, W. Articles by Szwech, P. Search for Related Content PubMed PubMed Citation Articles by Wiktor-Jedrzejczak, W. Articles by Szwech, P.

 Previous Article  |  Next Article 

Infect. Immun., 05 1996, 1577-1581, Vol 64, No. 5
Copyright © 1996, American Society for Microbiology

Colony-stimulating factor 1-dependent resident macrophages play a regulatory role in fighting Escherichia coli fecal peritonitis

W Wiktor-Jedrzejczak, B Dzwigala, M Szperl, M Maruszynski, E Urbanowska and P Szwech
Department of Immunology, Central Clinical Hospital, Military School of Medicine, Warsaw, Poland.

Osteopetrotic op/op mice have less than 5% of the normal number of macrophages in the peritoneal cavity (W. Wiktor-Jedrzejczak, A. Ahmed, C. Szczylik, and R.R. Skelly, J. Exp. Med. 156:1516-1527, 1982). Fecal peritonitis was induced by intraperitoneal injection of 0.5 ml of 5% autoclaved feces in saline along with Escherichia coli grown from feces of mice of the same colony and added in doses ranging between 10 and 10(6) CFU. Such infection led to a septic shock and either was lethal within 24 h or became cured without additional treatment of the mice. The op/op mice survived administration of 30-times-smaller doses of bacteria compared with their normal littermates. Analysis of the kinetics of cellular changes in the peritoneal cavity associated with such infection revealed that this increased susceptibility of macrophage-deficient mice cannot be explained by a direct role of macrophages in combating the infection. Instead, it appeared that the increased susceptibility to fatal fecal peritonitis was most likely due to delayed and impaired recruitment of neutrophils to the site of infection in mutant mice. The increased susceptibility of the op/op mice to E. coli fecal peritonitis was not due to their possible increased sensitivity to endotoxin, since the mutant mice tolerated lipopolysaccharide doses more than twice those tolerated by control littermates. On the other hand, their susceptibility to exogenous tumor necrosis factor alpha and interleukin-1 alpha was increased. Both mutant op/op and control mice were able to survive secondary challenge with 10(6) E. coli (administered along with feces) lethal for both types of mice on primary challenge. These data suggest that colony- stimulating factor 1-dependent resident peritoneal macrophages play a role in controlling primary infection by recruiting neutrophils and are not required for efficient response to secondary infection.

This article has been cited by other articles:

• Conway, J. G., Pink, H., Bergquist, M. L., Han, B., Depee, S., Tadepalli, S., Lin, P., Crumrine, R. C., Binz, J., Clark, R. L., Selph, J. L., Stimpson, S. A., Hutchins, J. T., Chamberlain, S. D., Brodie, T. A. (2008). Effects of the cFMS Kinase Inhibitor 5-(3-Methoxy-4-((4-methoxybenzyl)oxy)benzyl)pyrimidine-2,4-diamine (GW2580) in Normal and Arthritic Rats. J. Pharmacol. Exp. Ther. 326: 41-50 [Abstract] [Full Text]  
• Wehner, S., Behrendt, F. F, Lyutenski, B. N, Lysson, M., Bauer, A. J, Hirner, A., Kalff, J. C (2007). Inhibition of macrophage function prevents intestinal inflammation and postoperative ileus in rodents. Gut 56: 176-185 [Abstract] [Full Text]  
• Albee, L., Shi, B., Perlman, H. (2007). Aspartic protease and caspase 3/7 activation are central for macrophage apoptosis following infection with Escherichia coli. J. Leukoc. Biol. 81: 229-237 [Abstract] [Full Text]  
• Schonlau, F., Schlesiger, C., Ehrehen, J., Grabbe, S., Sorg, C., Sunderkotter, C. (2003). Monocyte and macrophage functions in M-CSF-deficient op/op mice during experimental leishmaniasis. J. Leukoc. Biol. 73: 564-573 [Abstract] [Full Text]  
• Jin, Y., Dons, L., Kristensson, K., Rottenberg, M. E. (2002). Colony-Stimulating Factor 1-Dependent Cells Protect against Systemic Infection with Listeria monocytogenes but Facilitate Neuroinvasion. Infect. Immun. 70: 4682-4686 [Abstract] [Full Text]  
• Guleria, I., Pollard, J. W. (2001). Aberrant Macrophage and Neutrophil Population Dynamics and Impaired Th1 Response to Listeria monocytogenes in Colony-Stimulating Factor 1-Deficient Mice. Infect. Immun. 69: 1795-1807 [Abstract] [Full Text]