This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Landmann, R. Articles by Zimmerli, W. Search for Related Content PubMed PubMed Citation Articles by Landmann, R. Articles by Zimmerli, W.

 Previous Article  |  Next Article 

Infect. Immun., 05 1996, 1762-1769, Vol 64, No. 5
Copyright © 1996, American Society for Microbiology

Human monocyte CD14 is upregulated by lipopolysaccharide

R Landmann, HP Knopf, S Link, S Sansano, R Schumann and W Zimmerli
Department of Research and Internal Medicine, University Hospital, Basel, Switzerland.

Membrane CD14 is involved in lipopolysaccharide (LPS)-induced monocyte activation; it binds LPS, and antibodies against CD14 block the effects of low-dose LPS. It is unknown how LPS regulates its own receptor CD14 in vitro. Therefore, we investigated the effects of LPS on CD14 mRNA and membrane and soluble CD14 (mCD14 and sCD14, respectively) in human monocytes and macrophages. No changes were observed during the first 3 h of LPS stimulation. After 6 to 15 h, LPS weakly reduced CD14 mRNA and mCD14 and transiently enhanced sCD14 release. A 2-day incubation with LPS caused increases in the levels of CD14 mRNA (2-fold), mCD14 (2- fold), sCD14 (1.5-fold), and LPS-fluorescein isothiocyanate binding (1.5-fold); a 5-h incubation with LPS was sufficient to induce the late effects on mCD14 and sCD14. The maximal effect on mCD14 and sCD14 was reached with > or = 1 ng of LPS per ml; the proportional distribution of the two sCD14 isoforms was not modified by LPS. Besides rough and smooth LPS, lipid A, heat-killed Escherichia coli, lipoteichoic acid, and Staphylococcus aureus cell wall extract (10 micrograms/ml) caused similar increases of mCD14. The LPS effect was blocked by polymyxin B but not by anti-tumor necrosis factor alpha, anti-interleukin-6, anti- gamma interferon, and anti-LPS-binding protein. LPS-induced tumor necrosis factor alpha production was abolished after a second 4-h challenge. In contrast, the LPS-induced increases CD14 mRNA, mCD14, and sCD14 were stronger and appeared earlier after a second LPS challenge. In conclusion, CD14 is transcriptionally upregulated by LPS and other bacterial cell wall constituents.

This article has been cited by other articles:

• Poulain-Godefroy, O., Lecoeur, C., Pattou, F., Fruhbeck, G., Froguel, P. (2008). Inflammation is associated with a decrease of lipogenic factors in omental fat in women. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295: R1-R7 [Abstract] [Full Text]  
• Brun, P., Castagliuolo, I., Leo, V. D., Buda, A., Pinzani, M., Palu, G., Martines, D. (2007). Increased intestinal permeability in obese mice: new evidence in the pathogenesis of nonalcoholic steatohepatitis. Am. J. Physiol. Gastrointest. Liver Physiol. 292: G518-G525 [Abstract] [Full Text]  
• Venet, F., Pachot, A., Debard, A.-L., Bohe, J., Bienvenu, J., Lepape, A., Powell, W. S., Monneret, G. (2006). Human CD4+CD25+ Regulatory T Lymphocytes Inhibit Lipopolysaccharide-Induced Monocyte Survival through a Fas/Fas Ligand-Dependent Mechanism. J. Immunol. 177: 6540-6547 [Abstract] [Full Text]  
• Brun, P., Castagliuolo, I., Pinzani, M., Palu, G., Martines, D. (2005). Exposure to bacterial cell wall products triggers an inflammatory phenotype in hepatic stellate cells. Am. J. Physiol. Gastrointest. Liver Physiol. 289: G571-G578 [Abstract] [Full Text]  
• Lin, S.-M., Frevert, C. W., Kajikawa, O., Wurfel, M. M., Ballman, K., Mongovin, S., Wong, V. A., Selk, A., Martin, T. R. (2004). Differential Regulation of Membrane CD14 Expression and Endotoxin-Tolerance in Alveolar Macrophages. Am. J. Respir. Cell Mol. Bio. 31: 162-170 [Abstract] [Full Text]  
• Lee, J.-H., Del Sorbo, L., Uhlig, S., Porro, G. A., Whitehead, T., Voglis, S., Liu, M., Slutsky, A. S., Zhang, H. (2004). Intercellular Adhesion Molecule-1 Mediates Cellular Cross-Talk between Parenchymal and Immune Cells after Lipopolysaccharide Neutralization. J. Immunol. 172: 608-616 [Abstract] [Full Text]  
• Yamazaki, K., Ueki-Maruyama, K., Oda, T., Tabeta, K., Shimada, Y., Tai, H., Nakajima, T., Yoshie, H., Herawati, D., Seymour, G.J. (2003). Single-nucleotide Polymorphism in the CD14 Promoter and Periodontal Disease Expression in a Japanese Population. J. Dent. Res. 82: 612-616 [Abstract] [Full Text]  
• Creery, D., Angel, J. B., Aucoin, S., Weiss, W., Cameron, W. D., Diaz-Mitoma, F., Kumar, A. (2002). Nef Protein of Human Immunodeficiency Virus and Lipopolysaccharide Induce Expression of CD14 on Human Monocytes through Differential Utilization of Interleukin-10. CVI 9: 1212-1221 [Abstract] [Full Text]  
• LeVan, T. D., Bloom, J. W., Bailey, T. J., Karp, C. L., Halonen, M., Martinez, F. D., Vercelli, D. (2001). A Common Single Nucleotide Polymorphism in the CD14 Promoter Decreases the Affinity of Sp Protein Binding and Enhances Transcriptional Activity. J. Immunol. 167: 5838-5844 [Abstract] [Full Text]  
• Alexander, C., Rietschel, E. Th. (2001). Invited review: Bacterial lipopolysaccharides and innate immunity. Innate Immunity 7: 167-202 [Abstract]  
• Du, X., Low, M. G. (2001). Down-Regulation of Glycosylphosphatidylinositol-Specific Phospholipase D Induced by Lipopolysaccharide and Oxidative Stress in the Murine Monocyte- Macrophage Cell Line RAW 264.7. Infect. Immun. 69: 3214-3223 [Abstract] [Full Text]  
• NOWACK, R., SCHWALBE, K., FLORES-SUÁREZ, L.-F., YARD, B., VAN DER WOUDE, F. J. (2000). Upregulation of CD14 and CD18 on Monocytes In Vitro by Antineutrophil Cytoplasmic Autoantibodies. J. Am. Soc. Nephrol. 11: 1639-1646 [Abstract] [Full Text]  
• Hess, C., Sadallah, S., Hefti, A., Landmann, R., Schifferli, J.-A. (1999). Ectosomes Released by Human Neutrophils Are Specialized Functional Units. J. Immunol. 163: 4564-4573 [Abstract] [Full Text]  
• Wyant, T. L., Tanner, M. K., Sztein, M. B. (1999). Salmonella typhi Flagella Are Potent Inducers of Proinflammatory Cytokine Secretion by Human Monocytes. Infect. Immun. 67: 3619-3624 [Abstract] [Full Text]  
• Lukkari, T. A., Jarvelainen, H. A., Oinonen, T., Kettunen, E., Lindros, K. O. (1999). SHORT-TERM ETHANOL EXPOSURE INCREASES THE EXPRESSION OF KUPFFER CELL CD14 RECEPTOR AND LIPOPOLYSACCHARIDE BINDING PROTEIN IN RAT LIVER. Alcohol Alcohol 34: 311-319 [Abstract] [Full Text]  
• Cauwels, A., Frei, K., Sansano, S., Fearns, C., Ulevitch, R., Zimmerli, W., Landmann, R. (1999). The Origin and Function of Soluble CD14 in Experimental Bacterial Meningitis. J. Immunol. 162: 4762-4772 [Abstract] [Full Text]  
• Hayashi, J., Masaka, T., Ishikawa, I. (1999). Increased Levels of Soluble CD14 in Sera of Periodontitis Patients. Infect. Immun. 67: 417-420 [Abstract] [Full Text]  
• Yu, W., Soprana, E., Cosentino, G., Volta, M., Lichenstein, H. S., Viale, G., Vercelli, D. (1998). Soluble CD141-152 Confers Responsiveness to Both Lipoarabinomannan and Lipopolysaccharide in a Novel HL-60 Cell Bioassay. J. Immunol. 161: 4244-4251 [Abstract] [Full Text]  
• Lien, E., Aukrust, P., Sundan, A., Muller, F., Froland, S. S., Espevik, T. (1998). Elevated Levels of Serum-Soluble CD14 in Human Immunodeficiency Virus Type 1 (HIV-1) Infection: Correlation to Disease Progression and Clinical Events. Blood 92: 2084-2092 [Abstract] [Full Text]  
• Sugawara, S., Sugiyama, A., Nemoto, E., Rikiishi, H., Takada, H. (1998). Heterogeneous Expression and Release of CD14 by Human Gingival Fibroblasts: Characterization and CD14-Mediated Interleukin-8 Secretion in Response to Lipopolysaccharide. Infect. Immun. 66: 3043-3049 [Abstract] [Full Text]  
• Heinzelmann, M., Mercer-Jones, M. A., Flodgaard, H., Miller, F. N. (1998). Heparin-Binding Protein (CAP37) Is Internalized in Monocytes and Increases LPS-Induced Monocyte Activation. J. Immunol. 160: 5530-5536 [Abstract] [Full Text]  
• Fingerle-Rowson, G., Auers, J., Kreuzer, E., Labeta, M., Schmidt, B., Samtleben, W., Ziegler-Heitbrock, H. W. L., Blumenstein, M. (1998). Down-regulation of surface monocyte lipopolysaccharide-receptor cd14in patients on cardiopulmonary bypass undergoing aorta-coronary bypass operation. J. Thorac. Cardiovasc. Surg. 115: 1172-1178 [Abstract] [Full Text]