This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Spinola, S. M. Articles by Shanks, K. L. Search for Related Content PubMed PubMed Citation Articles by Spinola, S. M. Articles by Shanks, K. L.

 Previous Article  |  Next Article 

Infect. Immun., Jun 1996, 1950-1955, Vol 64, No. 6
Copyright © 1996, American Society for Microbiology

The conserved 18,000-molecular-weight outer membrane protein of Haemophilus ducreyi has homology to PAL

SM Spinola, TJ Hiltke, K Fortney and KL Shanks
Department of Medicine, School of Medicine, Indiana University, Indianapolis 46202, USA.

Haemophilus ducreyi expresses an 18,000-molecular-weight outer membrane protein that contains a conserved surface-exposed epitope recognized by monoclonal antibody 3B9. Monoclonal antibody 3B9 cross-reacts with proteins of similar molecular weight found in many Haemophilus sp. strains, including P6, a candidate vaccine for Haemophilus influenzae. The gene encoding the 18,000-molecular-weight outer membrane protein was identified by screening a lambdagt11 genomic library with 3B9. The coding sequence of the gene was localized to a 471-bp open reading frame, designated pal (peptidoglycan-associated lipoprotein). Translation of pal predicted a mature polypeptide with a molecular weight of 15,000 that had extensive homology with P6 and Escherichia coli PAL. The predicted signal peptide had features characteristic of a prokaryotic lipoprotein, and processing of PAL was sensitive to globomycin in H. ducreyi. The sequences encoding mature H. ducreyi PAL were subcloned into the vector pRSET B and expressed as a polyhistidine- containing fusion protein that bound 3B9. In Western blot (immunoblot) analysis, serum samples obtained from healthy subjects and patients with chancroid or other genital ulcer diseases contained antibodies to purified PAL. Antibodies that bound to PAL were removed by absorption with a lysate of Haemophilus sp. antigens, suggesting that patients with chancroid do not develop an H. ducreyi-specific antibody response to PAL.

This article has been cited by other articles:

• Cascales, E., Buchanan, S. K., Duche, D., Kleanthous, C., Lloubes, R., Postle, K., Riley, M., Slatin, S., Cavard, D. (2007). Colicin Biology. Microbiol. Mol. Biol. Rev. 71: 158-229 [Abstract] [Full Text]  
• Paul-Satyaseela, M., Karched, M., Bian, Z., Ihalin, R., Boren, T., Arnqvist, A., Chen, C., Asikainen, S. (2006). Immunoproteomics of Actinobacillus actinomycetemcomitans outer-membrane proteins reveal a highly immunoreactive peptidoglycan-associated lipoprotein.. J Med Microbiol 55: 931-942 [Abstract] [Full Text]  
• Dubuisson, J.-F., Vianney, A., Hugouvieux-Cotte-Pattat, N., Lazzaroni, J. C. (2005). Tol-Pal proteins are critical cell envelope components of Erwinia chrysanthemi affecting cell morphology and virulence. Microbiology 151: 3337-3347 [Abstract] [Full Text]  
• White, C. D., Leduc, I., Olsen, B., Jeter, C., Harris, C., Elkins, C. (2005). Haemophilus ducreyi Outer Membrane Determinants, Including DsrA, Define Two Clonal Populations. Infect. Immun. 73: 2387-2399 [Abstract] [Full Text]  
• Leduc, I., Richards, P., Davis, C., Schilling, B., Elkins, C. (2004). A Novel Lectin, DltA, Is Required for Expression of a Full Serum Resistance Phenotype in Haemophilus ducreyi. Infect. Immun. 72: 3418-3428 [Abstract] [Full Text]  
• Ward, C. K., Mock, J. R., Hansen, E. J. (2004). The LspB Protein Is Involved in the Secretion of the LspA1 and LspA2 Proteins by Haemophilus ducreyi. Infect. Immun. 72: 1874-1884 [Abstract] [Full Text]  
• Vakevainen, M., Greenberg, S., Hansen, E. J. (2003). Inhibition of Phagocytosis by Haemophilus ducreyi Requires Expression of the LspA1 and LspA2 Proteins. Infect. Immun. 71: 5994-6003 [Abstract] [Full Text]  
• Ward, C. K., Latimer, J. L., Nika, J., Vakevainen, M., Mock, J. R., Deng, K., Blick, R. J., Hansen, E. J. (2003). Mutations in the lspA1 and lspA2 Genes of Haemophilus ducreyi Affect the Virulence of This Pathogen in an Animal Model System. Infect. Immun. 71: 2478-2486 [Abstract] [Full Text]  
• Nika, J. R., Latimer, J. L., Ward, C. K., Blick, R. J., Wagner, N. J., Cope, L. D., Mahairas, G. G., Munson, R. S. Jr., Hansen, E. J. (2002). Haemophilus ducreyi Requires the flp Gene Cluster for Microcolony Formation In Vitro. Infect. Immun. 70: 2965-2975 [Abstract] [Full Text]  
• Thomas, K. L., Leduc, I., Olsen, B., Thomas, C. E., Cameron, D. W., Elkins, C. (2001). Cloning, Overexpression, Purification, and Immunobiology of an 85-Kilodalton Outer Membrane Protein from Haemophilus ducreyi. Infect. Immun. 69: 4438-4446 [Abstract] [Full Text]  
• Throm, R. E., Spinola, S. M. (2001). Transcription of Candidate Virulence Genes of Haemophilus ducreyi during Infection of Human Volunteers. Infect. Immun. 69: 1483-1487 [Abstract] [Full Text]  
• Fortney, K. R., Young, R. S., Bauer, M. E., Katz, B. P., Hood, A. F., Munson, R. S. Jr., Spinola, S. M. (2000). Expression of Peptidoglycan-Associated Lipoprotein Is Required for Virulence in the Human Model of Haemophilus ducreyi Infection. Infect. Immun. 68: 6441-6448 [Abstract] [Full Text]  
• Llamas, M. A., Ramos, J. L., Rodríguez-Herva, J. J. (2000). Mutations in Each of the tol Genes of Pseudomonas putida Reveal that They Are Critical for Maintenance of Outer Membrane Stability. J. Bacteriol. 182: 4764-4772 [Abstract] [Full Text]  
• Elkins, C., Yi, K., Olsen, B., Thomas, C., Thomas, K., Morse, S. (2000). Development of a Serological Test for Haemophilus ducreyi for Seroprevalence Studies. J. Clin. Microbiol. 38: 1520-1526 [Abstract] [Full Text]  
• Ward, C. K., Lumbley, S. R., Latimer, J. L., Cope, L. D., Hansen, E. J. (1998). Haemophilus ducreyi Secretes a Filamentous Hemagglutinin-Like Protein. J. Bacteriol. 180: 6013-6022 [Abstract] [Full Text]  
• Cope, L. D., Lumbley, S., Latimer, J. L., Klesney-Tait, J., Stevens, M. K., Johnson, L. S., Purven, M., Munson, R. S. Jr., Lagergard, T., Radolf, J. D., Hansen, E. J. (1997). A diffusible cytotoxin of Haemophilus ducreyi. Proc. Natl. Acad. Sci. USA 94: 4056-4061 [Abstract] [Full Text]