This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schulte, R. Articles by Cornelis, G. R. Search for Related Content PubMed PubMed Citation Articles by Schulte, R. Articles by Cornelis, G. R.

 Previous Article  |  Next Article 

Infect. Immun., Jun 1996, 2106-2113, Vol 64, No. 6
Copyright © 1996, American Society for Microbiology

Differential secretion of interleukin-8 by human epithelial cell lines upon entry of virulent or nonvirulent Yersinia enterocolitica

R Schulte, P Wattiau, EL Hartland, RM Robins-Browne and GR Cornelis
Microbial Pathogenesis Unit, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.

Epithelial cells of the intestinal mucosa are among the first cells encountered by invasive pathogens. Bacterial invasion of the mucosa gives rise to an inflammatory response, characterized by the influx of polymorphonuclear leukocytes. The chemotactic stimulus responsible for this accumulation is unknown, but several in vitro studies have demonstrated that epithelial cells secrete the chemokine interleukin-8 (IL-8), a potent chemoattractant of polymorphonuclear leukocytes, upon bacterial entry. In this study we analyzed the secretion of IL-8 by human intestinal (T84) and cervical (HeLa) epithelial cell lines in response to infection with the enteric pathogen Yersinia enterocolitica. IL-8 was secreted by T84 and HeLa cells in response to invasion by Y. enterocolitica. Virulent Y. enterocolitica induced a significantly lower level of IL-8 secretion than nonvirulent Y. enterocolitica. Subsequent analysis employing a mutant defective in Yop secretion and various yop mutants showed that the reduced secretion of IL-8 is due to the presence of Yop proteins. Our data suggest that YopB and YopD are required for the suppressive effect.

This article has been cited by other articles:

• Pearson, M. M., Mobley, H. L. T. (2007). The type III secretion system of Proteus mirabilis HI4320 does not contribute to virulence in the mouse model of ascending urinary tract infection. J Med Microbiol 56: 1277-1283 [Abstract] [Full Text]  
• McNally, A., Dalton, T., Ragione, R. M. L., Stapleton, K., Manning, G., Newell, D. G. (2006). Yersinia enterocolitica isolates of differing biotypes from humans and animals are adherent, invasive and persist in macrophages, but differ in cytokine secretion profiles in vitro.. J Med Microbiol 55: 1725-1734 [Abstract] [Full Text]  
• Sharma, R., Tesfay, S., Tomson, F. L., Kanteti, R. P., Viswanathan, V. K., Hecht, G. (2006). Balance of bacterial pro- and anti-inflammatory mediators dictates net effect of enteropathogenic Escherichia coli on intestinal epithelial cells. Am. J. Physiol. Gastrointest. Liver Physiol. 290: G685-G694 [Abstract] [Full Text]  
• Schmid, Y., Grassl, G. A., Buhler, O. T., Skurnik, M., Autenrieth, I. B., Bohn, E. (2004). Yersinia enterocolitica Adhesin A Induces Production of Interleukin-8 in Epithelial Cells. Infect. Immun. 72: 6780-6789 [Abstract] [Full Text]  
• Erfurth, S. E., Grobner, S., Kramer, U., Gunst, D. S. J., Soldanova, I., Schaller, M., Autenrieth, I. B., Borgmann, S. (2004). Yersinia enterocolitica Induces Apoptosis and Inhibits Surface Molecule Expression and Cytokine Production in Murine Dendritic Cells. Infect. Immun. 72: 7045-7054 [Abstract] [Full Text]  
• Huang, D. B., DuPont, H. L., Jiang, Z.-D., Carlin, L., Okhuysen, P. C. (2004). Interleukin-8 Response in an Intestinal HCT-8 Cell Line Infected with Enteroaggregative and Enterotoxigenic Escherichia coli. CVI 11: 548-551 [Abstract] [Full Text]  
• Colmegna, I., Cuchacovich, R., Espinoza, L. R. (2004). HLA-B27-Associated Reactive Arthritis: Pathogenetic and Clinical Considerations. Clin. Microbiol. Rev. 17: 348-369 [Abstract] [Full Text]  
• Haller, D., Russo, M. P., Sartor, R. B., Jobin, C. (2002). IKKbeta and Phosphatidylinositol 3-Kinase/Akt Participate in Non-pathogenic Gram-negative Enteric Bacteria-induced RelA Phosphorylation and NF-kappa B Activation in Both Primary and Intestinal Epithelial Cell Lines. J. Biol. Chem. 277: 38168-38178 [Abstract] [Full Text]  
• Denecker, G., Totemeyer, S., Mota, L. J., Troisfontaines, P., Lambermont, I., Youta, C., Stainier, I., Ackermann, M., Cornelis, G. R. (2002). Effect of Low- and High-Virulence Yersinia enterocolitica Strains on the Inflammatory Response of Human Umbilical Vein Endothelial Cells. Infect. Immun. 70: 3510-3520 [Abstract] [Full Text]  
• Cornelis, G. R. (2000). Molecular and cell biology aspects of plague. Proc. Natl. Acad. Sci. USA 97: 8778-8783 [Abstract] [Full Text]  
• SCHULTE, R., GRASSL, G. A., PREGER, S., FESSELE, S., JACOBI, C. A., SCHALLER, M., NELSON, P. J., AUTENRIETH, I. B. (2000). Yersinia enterocolitica invasin protein triggers IL-8 production in epithelial cells via activation of Rel p65-p65 homodimers. FASEB J. 14: 1471-1484 [Abstract] [Full Text]  
• Haller, D, Bode, C, Hammes, W P, Pfeifer, A M A, Schiffrin, E J, Blum, S (2000). Non-pathogenic bacteria elicit a differential cytokine response by intestinal epithelial cell/leucocyte co-cultures. Gut 47: 79-87 [Abstract] [Full Text]  
• Kampik, D., Schulte, R., Autenrieth, I. B. (2000). Yersinia enterocolitica Invasin Protein Triggers Differential Production of Interleukin-1, Interleukin-8, Monocyte Chemoattractant Protein 1, Granulocyte-Macrophage Colony-Stimulating Factor, and Tumor Necrosis Factor Alpha in Epithelial Cells: Implications for Understanding the Early Cytokine Network in Yersinia Infections. Infect. Immun. 68: 2484-2492 [Abstract] [Full Text]  
• Wang, B., Kraig, E., Kolodrubetz, D. (2000). Use of Defined Mutants To Assess the Role of the Campylobacter rectus S-Layer in Bacterium-Epithelial Cell Interactions. Infect. Immun. 68: 1465-1473 [Abstract] [Full Text]  
• Cornelis, G. R., Boland, A., Boyd, A. P., Geuijen, C., Iriarte, M., Neyt, C., Sory, M.-P., Stainier, I. (1998). The Virulence Plasmid of Yersinia, an Antihost Genome. Microbiol. Mol. Biol. Rev. 62: 1315-1352 [Abstract] [Full Text]  
• Schmiel, D. H., Wagar, E., Karamanou, L., Weeks, D., Miller, V. L. (1998). Phospholipase A of Yersinia enterocolitica Contributes to Pathogenesis in a Mouse Model. Infect. Immun. 66: 3941-3951 [Abstract] [Full Text]  
• Wilson, M., Seymour, R., Henderson, B. (1998). Bacterial Perturbation of Cytokine Networks. Infect. Immun. 66: 2401-2409 [Full Text]  
• Sarker, M. R., Sory, M.-P., Boyd, A. P., Iriarte, M., Cornelis, G. R. (1998). LcrG is Required for Efficient Translocation of Yersinia Yop Effector Proteins into Eukaryotic Cells. Infect. Immun. 66: 2976-2979 [Abstract] [Full Text]  
• Hueck, C. J. (1998). Type III Protein Secretion Systems in Bacterial Pathogens of Animals and Plants. Microbiol. Mol. Biol. Rev. 62: 379-433 [Abstract] [Full Text]  
• Boland, A., Cornelis, G. R. (1998). Role of YopP in Suppression of Tumor Necrosis Factor Alpha Release by Macrophages during Yersinia Infection. Infect. Immun. 66: 1878-1884 [Abstract] [Full Text]  
• Schulte, R., Autenrieth, I. B. (1998). Yersinia enterocolitica-Induced Interleukin-8 Secretion by Human Intestinal Epithelial Cells Depends on Cell Differentiation. Infect. Immun. 66: 1216-1224 [Abstract] [Full Text]  
• Ruckdeschel, K., Machold, J., Roggenkamp, A., Schubert, S., Pierre, J., Zumbihl, R., Liautard, J.-P., Heesemann, J., Rouot, B. (1997). Yersinia enterocolitica Promotes Deactivation of Macrophage Mitogen-activated Protein Kinases Extracellular Signal-regulated Kinase-1/2, p38, and c-Jun NH2-terminal Kinase. CORRELATION WITH ITS INHIBITORY EFFECT ON TUMOR NECROSIS FACTOR-alpha PRODUCTION. J. Biol. Chem. 272: 15920-15927 [Abstract] [Full Text]  
• Dukuzumuremyi, J.-M., Rosqvist, R., Hallberg, B., Akerstrom, B., Wolf-Watz, H., Schesser, K. (2000). The Yersinia Protein Kinase A Is a Host Factor Inducible RhoA/Rac-binding Virulence Factor. J. Biol. Chem. 275: 35281-35290 [Abstract] [Full Text]