IAI FigSearch
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eaton, K. A.
Right arrow Articles by Krakowka, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eaton, K. A.
Right arrow Articles by Krakowka, S.

 Previous Article  |  Next Article 

Infect. Immun., 07 1996, 2445-2448, Vol 64, No. 7
Copyright © 1996, American Society for Microbiology

Colonization of gnotobiotic piglets by Helicobacter pylori deficient in two flagellin genes

KA Eaton, S Suerbaum, C Josenhans and S Krakowka
Ohio State University, Columbus 43210, USA.

Helicobacterpylori possesses two flagellin molecules, MA, the major species, and FlaB, which is expressed in minor amounts. This study sought to determine if one or both flagellin species are necessary for colonization or persistence by H. pylon. Thirty-six gnotobiotic piglets from six litters were given one of four isogenic strains of H. pylon orally. The bacterial strains used were strain N6, the wild type, which produced both FlaA and FlaB and was fully motile; N6flaB::km, which produced FlaA but not FlaB and was weakly motile; N6flaA::km, which expressed FlaB but not FlaA and was nonmotile; and N6flaA::cat/flaB::km, which produced neither flagellin and was nonmotile. Strain N6 colonized all piglets and persisted for 2, 4, and 10 days after inoculation. Both N6flaA::km and N6flaB::km colonized for 2 and 4 but not 10 days, and colonization was weak. N6flaA::cat/flaB:: km colonized for 2 days but did not persist for 4 or 10 days after inoculation. These findings demonstrate that both flagellin species are necessary for full colonization by H. pylon. Colonization for up to 4 days is possible in the absence of either flagellin species but not both.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 1996 by the American Society for Microbiology. All rights reserved.