This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Valvatne, H. Articles by Grewal, H. M. Search for Related Content PubMed PubMed Citation Articles by Valvatne, H. Articles by Grewal, H. M.

 Previous Article  |  Next Article 

Infect. Immun., 07 1996, 2635-2642, Vol 64, No. 7
Copyright © 1996, American Society for Microbiology

Identification and characterization of CS20, a new putative colonization factor of enterotoxigenic Escherichia coli

H Valvatne, H Sommerfelt, W Gaastra, MK Bhan and HM Grewal
Centre for Internation Health, University of Bergen, Norway. Havard. Valvatne@bio.uib.no

An enterotoxigenic Escherichia coli (ETEC) strain producing a previously undescribed putative colonization factor was isolated from a child with diarrhea in India. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of bacterial heat extracts revealed a polypeptide band of 20.8 kDa when the bacteria were grown at 37 degrees C which was absent after growth at 22 degrees C. A specific rabbit antiserum raised against the purified 20.8-kDa protein bound specifically to the fimbriae, as shown by immunoelectron microscopy, and inhibited bacterial adhesion to tissue-cultured Caco-2 cells. Transformation with a recombinant plasmid harboring the cfaD gene, which encodes a positive regulator for several ETEC fimbriae, induced hyperexpression of the 20.8-kDa fimbrial subunit and a substantial increase in the proportion of bacterial cells that were fimbriated. The N-terminal amino acid sequence of the polypeptide showed 65 and 60% identity to the PCFO20 and 987P fimbriae of human and porcine ETEC, respectively. We propose the term CS20 for this new putative colonization factor of human ETEC.

This article has been cited by other articles:

• Zhu, G., Chen, H., Choi, B.-K., Del Piero, F., Schifferli, D. M. (2005). Histone H1 Proteins Act As Receptors for the 987P Fimbriae of Enterotoxigenic Escherichia coli. J. Biol. Chem. 280: 23057-23065 [Abstract] [Full Text]  
• Khalil, S. B., Cassels, F. J., Shaheen, H. I., Pannell, L. K., El-Ghorab, N., Kamal, K., Mansour, M., Savarino, S. J., Peruski, L. F. Jr. (1999). Characterization of an Enterotoxigenic Escherichia coli Strain from Africa Expressing a Putative Colonization Factor. Infect. Immun. 67: 4019-4026 [Abstract] [Full Text]  
• Nataro, J. P., Kaper, J. B. (1998). Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11: 142-201 [Abstract] [Full Text]