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Infect. Immun., Oct 1997, 4337-4340, Vol 65, No. 10
Copyright © 1997, American Society for Microbiology

Survival of a bacterioferritin deletion mutant of Brucella melitensis 16M in human monocyte-derived macrophages

PA Denoel, RM Crawford, MS Zygmunt, A Tibor, VE Weynants, F Godfroid, DL Hoover and JJ Letesson
U. R. Biologie Moleculaire, Laboratoire de Microbiologie et d'Immunologie, Facultes Universitaires Notre Dame de la Paix, Namur, Belgium. Philippe.Denoel@fundp.ac.be

A bacterioferritin (BFR) deletion mutant of Brucella melitensis 16M was generated by gene replacement. The deletion was complemented with a broad-host-range vector carrying the wild-type bfr gene, pBBR-bfr. The survival and growth of the mutant, B. melitensis PAD 2-78, were similar to those of its parental strain in human monocyte-derived macrophages (MDM). These results suggest that BFR is not essential for the intracellular survival of B. melitensis in human MDM.

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