This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Saha, P. K. Articles by Nair, G. B. Search for Related Content PubMed PubMed Citation Articles by Saha, P. K. Articles by Nair, G. B.

 Previous Article  |  Next Article 

Infect. Immun., Feb 1997, 801-805, Vol 65, No. 2
Copyright © 1997, American Society for Microbiology

Production of monoclonal antibodies to the non-membrane-damaging cytotoxin (NMDCY) purified from Vibrio cholerae O26 and distribution of NMDCY among strains of Vibrio cholerae and other enteric bacteria determined by monoclonal-polyclonal sandwich enzyme-linked immunosorbent assay

PK Saha and GB Nair
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.

The distribution of a newly described secretogenic non-membrane- damaging cytotoxin (NMDCY) among strains of Vibrio cholerae and other enteric bacteria was determined. To accomplish this, monoclonal antibodies against NMDCY were prepared and a sandwich monoclonal- polyclonal enzyme-linked immunosorbent assay (ELISA) was developed. By the sandwich ELISA, it was determined that 55.6% of the 412 strains of V. cholerae examined produced NMDCY at varying concentrations while 76, 37.9, and 15.6% of the clinical strains of Vibrio parahaemolyticus, Aeromonas spp., and Shigella spp., respectively, produced NMDCY. Because of its enterotoxigenic potential and based on its widespread distribution among strains of V. cholerae, we believe that NMDCY may constitute an important virulence determinant in the cascade of events which enable the organism to precipitate the disease.

This article has been cited by other articles:

• Sharma, C., Thungapathra, M., Ghosh, A., Mukhopadhyay, A. K., Basu, A., Mitra, R., Basu, I., Bhattacharya, S. K., Shimada, T., Ramamurthy, T., Takeda, T., Yamasaki, S., Takeda, Y., Nair, G. B. (1998). Molecular Analysis of Non-O1, Non-O139 Vibrio cholerae Associated with an Unusual Upsurge in the Incidence of Cholera-Like Disease in Calcutta, India. J. Clin. Microbiol. 36: 756-763 [Abstract] [Full Text]