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Infect. Immun., 04 1997, 1546-1549, Vol 65, No. 4
Copyright © 1997, American Society for Microbiology

Binding of the type 3 fimbriae of Klebsiella pneumoniae to human endothelial and urinary bladder cells

AM Tarkkanen, R Virkola, S Clegg and TK Korhonen
Department of Biosciences, FIN-00014 University of Helsinki, Finland.

Binding of the two identified type 3 fimbrial variants of Klebsiella pneumoniae to human endothelial EA-hy926 and bladder T24 cells was assessed. The recombinant Escherichia coli strain LE392(pFK12), expressing plasmid-encoded type 3 fimbriae of K. pneumoniae, adhered to both cell lines, and the fimbriae purified from the strain bound to both cell lines in a dose-dependent manner. Adhesiveness to both cell lines of chromosomally encoded type 3 fimbriae from K. pneumoniae IApc35 was lower. No binding was detected with type 1 fimbriae of K. pneumoniae. Both type 3 fimbrial variants exhibited a significantly lower affinity for the cell lines than did S fimbriae of meningitis- associated E. coli.

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