This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Soares, I. S. Articles by Rodrigues, M. M. Search for Related Content PubMed PubMed Citation Articles by Soares, I. S. Articles by Rodrigues, M. M.

 Previous Article  |  Next Article 

Infect. Immun., 05 1997, 1606-1614, Vol 65, No. 5
Copyright © 1997, American Society for Microbiology

Acquired immune responses to the N- and C-terminal regions of Plasmodium vivax merozoite surface protein 1 in individuals exposed to malaria

IS Soares, G Levitus, JM Souza, HA Del Portillo and MM Rodrigues
Departamento de Patologia, Centro de Ciencias Biologicas, Universidade Federal do Para, Brazil.

In this study, we evaluated the naturally acquired immune response to Plasmodium vivax merozoite surface protein 1 (PvMSP1) in individuals with recent clinical episodes of malaria from the state of Para, Brazil. Ten recombinant proteins representing the first 682 amino acids (aa) of the N-terminal region and one representing the final 111 aa of the C-terminal region were expressed in Escherichia coli as glutathione S-transferase fusion proteins. Both of these regions have been suggested as candidates for development of a vaccine against Plasmodium sp. The total frequencies of individuals with antibodies and cellular immune responses to PvMSP1 were high (83.8 and 75%, respectively). The recombinant proteins representing the N- and C-terminal regions were recognized by 51.4 and 64.1% of sera, respectively. The frequency of responders to the C-terminal region increased according to the number of previous malaria episodes, reaching 83.3% after four episodes. Cellular immune response was measured by in vitro proliferation and gamma interferon production. Peripheral blood mononuclear cells of 75 and 47.2% of individuals proliferated in response to stimulation by the N- and C-terminal regions, respectively. Also, we found that one protein representing the N terminus and a second representing the C terminus of PvMSP1 stimulated 54.5% of individuals to secrete gamma interferon. We concluded that PvMSP1 is immunogenic to a large proportion of individuals exposed to malaria. Our results also suggested that the C-terminal region of PvMSP1 containing the two epidermal growth factor-like domains is particularly immunogenic to antibodies and T cells during natural infection in humans.

This article has been cited by other articles:

• Zeyrek, F. Y., Babaoglu, A., Demirel, S., Erdogan, D. D., Ak, M., Korkmaz, M., Coban, C. (2008). Analysis of Naturally Acquired Antibody Responses to the 19-kd C-Terminal Region of Merozoite Surface Protein-1 of Plasmodium vivax from Individuals in Sanliurfa, Turkey. Am J Trop Med Hyg 78: 729-732 [Abstract] [Full Text]  
• Bastos, M. S., da Silva-Nunes, M., Malafronte, R. S., Hoffmann, E. H. E., Wunderlich, G., Moraes, S. L., Ferreira, M. U. (2007). Antigenic Polymorphism and Naturally Acquired Antibodies to Plasmodium vivax Merozoite Surface Protein 1 in Rural Amazonians. CVI 14: 1249-1259 [Abstract] [Full Text]  
• Nogueira, P. A., Piovesan Alves, F., Fernandez-Becerra, C., Pein, O., Rodrigues Santos, N., Pereira da Silva, L. H., Plessman Camargo, E., del Portillo, H. A. (2006). A Reduced Risk of Infection with Plasmodium vivax and Clinical Protection against Malaria Are Associated with Antibodies against the N Terminus but Not the C Terminus of Merozoite Surface Protein 1.. Infect. Immun. 74: 2726-2733 [Abstract] [Full Text]  
• VALDERRAMA-AGUIRRE, A., QUINTERO, G., GOMEZ, A., CASTELLANOS, A., PEREZ, Y., MENDEZ, F., AREVALO-HERRERA, M., HERRERA, S. (2005). ANTIGENICITY, IMMUNOGENICITY, AND PROTECTIVE EFFICACY OF PLASMODIUM VIVAX MSP1 PV200L: A POTENTIAL MALARIA VACCINE SUBUNIT. Am J Trop Med Hyg 73: 16-24 [Abstract] [Full Text]  
• Caro-Aguilar, I., Rodriguez, A., Calvo-Calle, J. M., Guzman, F., De la Vega, P., Elkin Patarroyo, M., Galinski, M. R., Moreno, A. (2002). Plasmodium vivax Promiscuous T-Helper Epitopes Defined and Evaluated as Linear Peptide Chimera Immunogens. Infect. Immun. 70: 3479-3492 [Abstract] [Full Text]  
• Dutta, S., Ware, L. A., Barbosa, A., Ockenhouse, C. F., Lanar, D. E. (2001). Purification, Characterization, and Immunogenicity of a Disulfide Cross-Linked Plasmodium vivax Vaccine Candidate Antigen, Merozoite Surface Protein 1, Expressed in Escherichia coli. Infect. Immun. 69: 5464-5470 [Abstract] [Full Text]  
• Quin, S. J., Langhorne, J. (2001). Different Regions of the Malaria Merozoite Surface Protein 1 of Plasmodium chabaudi Elicit Distinct T-Cell and Antibody Isotype Responses. Infect. Immun. 69: 2245-2251 [Abstract] [Full Text]  
• Park, J.-W., Moon, S.-H., Yeom, J.-S., Lim, K.-J., Sohn, M.-J., Jung, W.-C., Cho, Y.-J., Jeon, K.-W., Ju, W., Ki, C.-S., Oh, M.-D., Choe, K. (2001). Naturally Acquired Antibody Responses to the C-Terminal Region of Merozoite Surface Protein 1 of Plasmodium vivax in Korea. CVI 8: 14-20 [Abstract] [Full Text]  
• Matsumoto, S., Yukitake, H., Kanbara, H., Yamada, T. (1998). Recombinant Mycobacterium bovis Bacillus Calmette-Guerin Secreting Merozoite Surface Protein 1 (MSP1) Induces Protection against Rodent Malaria Parasite Infection Depending on MSP1-stimulated Interferon gamma  and Parasite-specific Antibodies. J. Exp. Med. 188: 845-854 [Abstract] [Full Text]