This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dean-Nystrom, E. A. Articles by Moon, H. W. Search for Related Content PubMed PubMed Citation Articles by Dean-Nystrom, E. A. Articles by Moon, H. W.

 Previous Article  |  Next Article 

Infect. Immun., May 1997, 1842-1848, Vol 65, No. 5
Copyright © 1997, American Society for Microbiology

Pathogenicity of Escherichia coli O157:H7 in the intestines of neonatal calves

EA Dean-Nystrom, BT Bosworth, WC Cray Jr and HW Moon
Enteric Diseases and Food Safety Research Unit, National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa 50010, USA. enystrom@nadc.ars.usda.gov

Cattle are an important reservoir of Shiga toxin-producing enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains, foodborne pathogens that cause hemorrhagic colitis and hemolytic uremic syndrome in humans. EHEC O157:H7 strains are not pathogenic in calves >3 weeks old. Our objective was to determine if EHEC O157:H7 strains are pathogenic in neonatal calves. Calves <36 h old inoculated with EHEC O157:H7 developed diarrhea and enterocolitis with attaching and effacing (A/E) lesions in both the large and small intestines by 18 h postinoculation. The severity of diarrhea and inflammation, and also the frequency and extent of A/E lesions, increased by 3 days postinoculation. We conclude that EHEC O157:H7 strains are pathogenic in neonatal calves. The neonatal calf model is relevant for studying the pathogenesis of EHEC O157:H7 infections in cattle. It should also be useful for identifying ways to reduce EHEC O157:H7 infections in cattle and thus reduce the risk of EHEC O157:H7 disease in humans.

This article has been cited by other articles:

• Standley, T., Paterson, J., Skinner, K., Rainey, B., Roberts, A., Geary, T., Smith, G., White, R. (2008). The Use of an Experimental Vaccine in Gestating Beef Cows to Reduce the Shedding of Escherichia coli O157:H7 in the Newborn Calf. Professional Animal Scientist 24: 260-263 [Abstract]  
• Coombes, B. K., Wickham, M. E., Mascarenhas, M., Gruenheid, S., Finlay, B. B., Karmali, M. A. (2008). Molecular Analysis as an Aid To Assess the Public Health Risk of Non-O157 Shiga Toxin-Producing Escherichia coli Strains. Appl. Environ. Microbiol. 74: 2153-2160 [Abstract] [Full Text]  
• Aktan, I., La Ragione, R. M., Woodward, M. J. (2007). Colonization, Persistence, and Tissue Tropism of Escherichia coli O26 in Conventionally Reared Weaned Lambs. Appl. Environ. Microbiol. 73: 691-698 [Abstract] [Full Text]  
• Hoffman, M. A., Menge, C., Casey, T. A., Laegreid, W., Bosworth, B. T., Dean-Nystrom, E. A. (2006). Bovine Immune Response to Shiga-Toxigenic Escherichia coli O157:H7. CVI 13: 1322-1327 [Abstract] [Full Text]  
• Rashid, R. A., Tabata, T. A., Oatley, M. J., Besser, T. E., Tarr, P. I., Moseley, S. L. (2006). Expression of Putative Virulence Factors of Escherichia coli O157:H7 Differs in Bovine and Human Infections. Infect. Immun. 74: 4142-4148 [Abstract] [Full Text]  
• Low, A. S., Dziva, F., Torres, A. G., Martinez, J. L., Rosser, T., Naylor, S., Spears, K., Holden, N., Mahajan, A., Findlay, J., Sales, J., Smith, D. G. E., Low, J. C., Stevens, M. P., Gally, D. L. (2006). Cloning, Expression, and Characterization of Fimbrial Operon F9 from Enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 74: 2233-2244 [Abstract] [Full Text]  
• Wang, J.-Y., Wang, S.-S., Yin, P.-Z. (2006). Haemolytic-uraemic syndrome caused by a non-O157 : H7 Escherichia coli strain in experimentally inoculated dogs. J Med Microbiol 55: 23-29 [Abstract] [Full Text]  
• Naylor, S. W., Roe, A. J., Nart, P., Spears, K., Smith, David. G. E., Low, J. C., Gally, D. L. (2005). Escherichia coli O157 : H7 forms attaching and effacing lesions at the terminal rectum of cattle and colonization requires the LEE4 operon. Microbiology 151: 2773-2781 [Abstract] [Full Text]  
• van Diemen, P. M., Dziva, F., Stevens, M. P., Wallis, T. S. (2005). Identification of Enterohemorrhagic Escherichia coli O26:H- Genes Required for Intestinal Colonization in Calves. Infect. Immun. 73: 1735-1743 [Abstract] [Full Text]  
• Best, A., La Ragione, R. M., Sayers, A. R., Woodward, M. J. (2005). Role for Flagella but Not Intimin in the Persistent Infection of the Gastrointestinal Tissues of Specific-Pathogen-Free Chicks by Shiga Toxin-Negative Escherichia coli O157:H7. Infect. Immun. 73: 1836-1846 [Abstract] [Full Text]  
• Jordan, D. M., Booher, S. L., Moon, H. W. (2005). Escherichia coli O157:H7 Does Not Require Intimin To Persist in Pigs. Infect. Immun. 73: 1865-1867 [Full Text]  
• Jordan, D. M., Sperandio, V., Kaper, J. B., Dean-Nystrom, E. A., Moon, H. W. (2005). Colonization of Gnotobiotic Piglets by a luxS Mutant Strain of Escherichia coli O157:H7. Infect. Immun. 73: 1214-1216 [Abstract] [Full Text]  
• Hussein, H. S., Sakuma, T. (2005). Invited Review: Prevalence of Shiga Toxin-Producing Escherichia coli in Dairy Cattle and Their Products. J DAIRY SCI 88: 450-465 [Abstract] [Full Text]  
• Pohlenz, J. F., Winter, K. R., Dean-Nystrom, E. A. (2005). Shiga-Toxigenic Escherichia coli-Inoculated Neonatal Piglets Develop Kidney Lesions That Are Comparable to Those in Humans with Hemolytic-Uremic Syndrome. Infect. Immun. 73: 612-616 [Abstract] [Full Text]  
• Dziva, F., van Diemen, P. M., Stevens, M. P., Smith, A. J., Wallis, T. S. (2004). Identification of Escherichia coli O157 : H7 genes influencing colonization of the bovine gastrointestinal tract using signature-tagged mutagenesis. Microbiology 150: 3631-3645 [Abstract] [Full Text]  
• Jordan, D. M., Cornick, N., Torres, A. G., Dean-Nystrom, E. A., Kaper, J. B., Moon, H. W. (2004). Long Polar Fimbriae Contribute to Colonization by Escherichia coli O157:H7 In Vivo. Infect. Immun. 72: 6168-6171 [Abstract] [Full Text]  
• Cornick, N. A., Helgerson, A. F. (2004). Transmission and Infectious Dose of Escherichia coli O157:H7 in Swine. Appl. Environ. Microbiol. 70: 5331-5335 [Abstract] [Full Text]  
• Stevens, M. P., Roe, A. J., Vlisidou, I., van Diemen, P. M., La Ragione, R. M., Best, A., Woodward, M. J., Gally, D. L., Wallis, T. S. (2004). Mutation of toxB and a Truncated Version of the efa-1 Gene in Escherichia coli O157:H7 Influences the Expression and Secretion of Locus of Enterocyte Effacement-Encoded Proteins but not Intestinal Colonization in Calves or Sheep. Infect. Immun. 72: 5402-5411 [Abstract] [Full Text]  
• Menge, C., Stamm, I., van Diemen, P. M., Sopp, P., Baljer, G., Wallis, T. S., Stevens, M. P. (2004). Phenotypic and functional characterization of intraepithelial lymphocytes in a bovine ligated intestinal loop model of enterohaemorrhagic Escherichia coli infection. J Med Microbiol 53: 573-579 [Abstract] [Full Text]  
• Miranda, R. L., Conway, T., Leatham, M. P., Chang, D. E., Norris, W. E., Allen, J. H., Stevenson, S. J., Laux, D. C., Cohen, P. S. (2004). Glycolytic and Gluconeogenic Growth of Escherichia coli O157:H7 (EDL933) and E. coli K-12 (MG1655) in the Mouse Intestine. Infect. Immun. 72: 1666-1676 [Abstract] [Full Text]  
• Dean-Nystrom, E. A., Melton-Celsa, A. R., Pohlenz, J. F. L., Moon, H. W., O'Brien, A. D. (2003). Comparative Pathogenicity of Escherichia coli O157 and Intimin-Negative Non-O157 Shiga Toxin-Producing E. coli Strains in Neonatal Pigs. Infect. Immun. 71: 6526-6533 [Abstract] [Full Text]  
• Naylor, S. W., Low, J. C., Besser, T. E., Mahajan, A., Gunn, G. J., Pearce, M. C., McKendrick, I. J., Smith, D. G. E., Gally, D. L. (2003). Lymphoid Follicle-Dense Mucosa at the Terminal Rectum Is the Principal Site of Colonization of Enterohemorrhagic Escherichia coli O157:H7 in the Bovine Host. Infect. Immun. 71: 1505-1512 [Abstract] [Full Text]  
• Mizoguchi, K., Morita, M., Fischer, C. R., Yoichi, M., Tanji, Y., Unno, H. (2003). Coevolution of Bacteriophage PP01 and Escherichia coli O157:H7 in Continuous Culture. Appl. Environ. Microbiol. 69: 170-176 [Abstract] [Full Text]  
• Stevens, M. P., van Diemen, P. M., Dziva, F., Jones, P. W., Wallis, T. S. (2002). Options for the control of enterohaemorrhagic Escherichia coli in ruminants. Microbiology 148: 3767-3778 [Full Text]  
• COOKSON, A. L., WALES, A. D., ROE, J. M., HAYES, C. M., PEARSON, G. R., WOODWARD, M. J. (2002). Variation in the persistence of Escherichia coli O157:H7 in experimentally inoculated 6-week-old conventional lambs. J Med Microbiol 51: 1032-1040 [Abstract] [Full Text]  
• Stevens, M. P., van Diemen, P. M., Frankel, G., Phillips, A. D., Wallis, T. S. (2002). Efa1 Influences Colonization of the Bovine Intestine by Shiga Toxin-Producing Escherichia coli Serotypes O5 and O111. Infect. Immun. 70: 5158-5166 [Abstract] [Full Text]  
• WALES, A. D., CLIFTON-HADLEY, F. A., COOKSON, A. L., DIBB-FULLER, M. P., LARAGIONE, R. M., PEARSON, G. R., WOODWARD, M. J. (2002). Production of attaching-effacing lesions in ligated large intestine loops of 6-month-old sheep by Escherichia coli O157:H7. J Med Microbiol 51: 755-763 [Abstract] [Full Text]  
• Dean-Nystrom, E. A., Gansheroff, L. J., Mills, M., Moon, H. W., O'Brien, A. D. (2002). Vaccination of Pregnant Dams with IntiminO157 Protects Suckling Piglets from Escherichia coli O157:H7 Infection. Infect. Immun. 70: 2414-2418 [Abstract] [Full Text]  
• Cornick, N. A., Booher, S. L., Moon, H. W. (2002). Intimin Facilitates Colonization by Escherichia coli O157:H7 in Adult Ruminants. Infect. Immun. 70: 2704-2707 [Abstract] [Full Text]  
• Stevens, M. P., Marches, O., Campbell, J., Huter, V., Frankel, G., Phillips, A. D., Oswald, E., Wallis, T. S. (2002). Intimin, Tir, and Shiga Toxin 1 Do Not Influence Enteropathogenic Responses to Shiga Toxin-Producing Escherichia coli in Bovine Ligated Intestinal Loops. Infect. Immun. 70: 945-952 [Abstract] [Full Text]  
• WALES, A. D., PEARSON, G. R., SKUSE, A. M., ROE, J. M., HAYES, C. M., COOKSON, A. L., WOODWARD, M. J. (2001). Attaching and effacing lesions caused by Escherichia coli O157:H7 in experimentally inoculated neonatal lambs. J Med Microbiol 50: 752-758 [Abstract] [Full Text]  
• DIBB-FULLER, M.P., BEST, A., STAGG, D.A., COOLEY, W.A., WOODWARD, M.J. (2001). An in-vitro model for studying the interaction of Escherichia coli O157:H7 and other enteropathogens with bovine primary cell cultures. J Med Microbiol 50: 759-769 [Abstract] [Full Text]  
• Fischer, J. R., Zhao, T., Doyle, M. P., Goldberg, M. R., Brown, C. A., Sewell, C. T., Kavanaugh, D. M., Bauman, C. D. (2001). Experimental and Field Studies of Escherichia coli O157:H7 in White-Tailed Deer. Appl. Environ. Microbiol. 67: 1218-1224 [Abstract] [Full Text]  
• Cornick, N. A., Booher, S. L., Casey, T. A., Moon, H. W. (2000). Persistent Colonization of Sheep by Escherichia coli O157:H7 and Other E. coli Pathotypes. Appl. Environ. Microbiol. 66: 4926-4934 [Abstract] [Full Text]  
• Pruimboom-Brees, I. M., Morgan, T. W., Ackermann, M. R., Nystrom, E. D., Samuel, J. E., Cornick, N. A., Moon, H. W. (2000). Cattle lack vascular receptors for Escherichia coli O157:H7 Shiga toxins. Proc. Natl. Acad. Sci. USA 10.1073/pnas.190329997v1 [Abstract] [Full Text]  
• Magnuson, B. A., Davis, M., Hubele, S., Austin, P. R., Kudva, I. T., Williams, C. J., Hunt, C. W., Hovde, C. J. (2000). Ruminant Gastrointestinal Cell Proliferation and Clearance of Escherichia coli O157:H7. Infect. Immun. 68: 3808-3814 [Abstract] [Full Text]  
• Dean-Nystrom, E. A., Pohlenz, J. F. L., Moon, H. W., O'Brien, A. D. (2000). Escherichia coli O157:H7 Causes More-Severe Systemic Disease in Suckling Piglets than in Colostrum-Deprived Neonatal Piglets. Infect. Immun. 68: 2356-2358 [Abstract] [Full Text]  
• Price, S. B., Cheng, C.-M., Kaspar, C. W., Wright, J. C., DeGraves, F. J., Penfound, T. A., Castanie-Cornet, M.-P., Foster, J. W. (2000). Role of rpoS in Acid Resistance and Fecal Shedding of Escherichia coli O157:H7. Appl. Environ. Microbiol. 66: 632-637 [Abstract] [Full Text]  
• Oswald, E., Schmidt, H., Morabito, S., Karch, H., Marches, O., Caprioli, A. (2000). Typing of Intimin Genes in Human and Animal Enterohemorrhagic and Enteropathogenic Escherichia coli: Characterization of a New Intimin Variant. Infect. Immun. 68: 64-71 [Abstract] [Full Text]  
• Menge, C., Wieler, L. H., Schlapp, T., Baljer, G. (1999). Shiga Toxin 1 from Escherichia coli Blocks Activation and Proliferation of Bovine Lymphocyte Subpopulations In Vitro. Infect. Immun. 67: 2209-2217 [Abstract] [Full Text]  
• Dean-Nystrom, E. A., Bosworth, B. T., Moon, H. W., O'Brien, A. D. (1998). Escherichia coli O157:H7 Requires Intimin for Enteropathogenicity in Calves. Infect. Immun. 66: 4560-4563 [Abstract] [Full Text]  
• Paton, J. C., Paton, A. W. (1998). Pathogenesis and Diagnosis of Shiga Toxin-Producing Escherichia coli Infections. Clin. Microbiol. Rev. 11: 450-479 [Abstract] [Full Text]  
• Wieler, L. H., Schwanitz, A., Vieler, E., Busse, B., Steinrück, H., Kaper, J. B., Baljer, G. (1998). Virulence Properties of Shiga Toxin-Producing Escherichia coli (STEC) Strains of Serogroup O118, a Major Group of STEC Pathogens in Calves. J. Clin. Microbiol. 36: 1604-1607 [Abstract] [Full Text]  
• Shere, J. A., Bartlett, K. J., Kaspar, C. W. (1998). Longitudinal Study of Escherichia coli O157:H7 Dissemination on Four Dairy Farms in Wisconsin. Appl. Environ. Microbiol. 64: 1390-1399 [Abstract] [Full Text]  
• Pruimboom-Brees, I. M., Morgan, T. W., Ackermann, M. R., Nystrom, E. D., Samuel, J. E., Cornick, N. A., Moon, H. W. (2000). Cattle lack vascular receptors for Escherichia coli O157:H7 Shiga toxins. Proc. Natl. Acad. Sci. USA 97: 10325-10329 [Abstract] [Full Text]