This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fraser, T. Articles by Adams, J. H. Search for Related Content PubMed PubMed Citation Articles by Fraser, T. Articles by Adams, J. H.

 Previous Article  |  Next Article 

Infect. Immun., Jul 1997, 2772-2777, Vol 65, No. 7
Copyright © 1997, American Society for Microbiology

Expression and serologic activity of a soluble recombinant Plasmodium vivax Duffy binding protein

T Fraser, P Michon, JW Barnwell, AR Noe, F Al-Yaman, DC Kaslow and JH Adams
Department of Biological Sciences, University of Notre Dame, Indiana 46556, USA.

Plasmodium vivax Duffy binding protein (DBP) is a conserved functionally important protein. P. vivax DBP is an asexual blood-stage malaria vaccine candidate because adhesion of P. vivax DBP to its erythrocyte receptor is essential for the parasite to continue development in human blood. We developed a soluble recombinant protein of P. vivax DBP (rDBP) and examined serologic activity to it in residents of a region of high endemicity. This soluble rDBP product contained the cysteine-rich ligand domain and most of the contiguous proline-rich hydrophilic region. rDBP was expressed as a glutathione S- transferase (GST) fusion protein and was isolated from GST by thrombin treatment of the purified fusion protein bound on glutathione agarose beads. P. vivax rDBP was immunogenic in rabbits and induced antibodies that reacted with P. vivax and Plasmodium knowlesi merozoites. Human sera from adult residents of a region of Papua New Guinea where malaria is highly endemic or P. vivax-infected North American residents reacted with rDBP in an immunoblot and an enzyme-linked immunosorbent assay. The reactivity to reduced, denatured P. vivax rDBP and the cross- reactivity with P. knowlesi indicated the presence of immunogenic conserved linear B-cell epitopes. A more extensive serologic survey of Papua New Guinea residents showed that antibody response to P. vivax DBP is common and increases with age, suggesting a possible boosting of the antibody response in some by repeated exposure to P. vivax. A positive humoral response to P. vivax DBP correlated with a significantly higher response to P. vivax MSP-1(19). The natural immunogenicity of this DBP should strengthen its usefulness as a vaccine.

This article has been cited by other articles:

• AREVALO-HERRERA, M., CASTELLANOS, A., YAZDANI, S. S., SHAKRI, A. R., CHITNIS, C. E., DOMINIK, R., HERRERA, S. (2005). IMMUNOGENICITY AND PROTECTIVE EFFICACY OF RECOMBINANT VACCINE BASED ON THE RECEPTOR-BINDING DOMAIN OF THE PLASMODIUM VIVAX DUFFY BINDING PROTEIN IN AOTUS MONKEYS. Am J Trop Med Hyg 73: 25-31 [Abstract] [Full Text]  
• TRAN, T. M., OLIVEIRA-FERREIRA, J., MORENO, A., SANTOS, F., YAZDANI, S. S., CHITNIS, C. E., ALTMAN, J. D., MEYER, E. V-S., BARNWELL, J. W., GALINSKI, M. R. (2005). COMPARISON OF IgG REACTIVITIES TO PLASMODIUM VIVAX MEROZOITE INVASION ANTIGENS IN A BRAZILIAN AMAZON POPULATION. Am J Trop Med Hyg 73: 244-255 [Abstract] [Full Text]  
• CERAVOLO, I. P., BRUNA-ROMERO, O., BRAGA, E. M., FONTES, C. J. F., BRITO, C. F. A., SOUZA, J. M., KRETTLI, A. U., ADAMS, J. H., CARVALHO, L. H. (2005). ANTI-PLASMODIUM VIVAX DUFFY BINDING PROTEIN ANTIBODIES MEASURE EXPOSURE TO MALARIA IN THE BRAZILIAN AMAZON. Am J Trop Med Hyg 72: 675-681 [Abstract] [Full Text]  
• Xainli, J., Cole-Tobian, J. L., Baisor, M., Kastens, W., Bockarie, M., Yazdani, S. S., Chitnis, C. E., Adams, J. H., King, C. L. (2003). Epitope-Specific Humoral Immunity to Plasmodium vivax Duffy Binding Protein. Infect. Immun. 71: 2508-2515 [Abstract] [Full Text]  
• Xainli, J., Baisor, M., Kastens, W., Bockarie, M., Adams, J. H., King, C. L. (2002). Age-Dependent Cellular Immune Responses to Plasmodium vivax Duffy Binding Protein in Humans. J. Immunol. 169: 3200-3207 [Abstract] [Full Text]  
• Michon, P., Fraser, T., Adams, J. H. (2000). Naturally Acquired and Vaccine-Elicited Antibodies Block Erythrocyte Cytoadherence of the Plasmodium vivax Duffy Binding Protein. Infect. Immun. 68: 3164-3171 [Abstract] [Full Text]  
• Singh, S., Pandey, K., Chattopadhayay, R., Yazdani, S. S., Lynn, A., Bharadwaj, A., Ranjan, A., Chitnis, C. (2001). Biochemical, Biophysical, and Functional Characterization of Bacterially Expressed and Refolded Receptor Binding Domain of Plasmodium vivax Duffy-binding Protein. J. Biol. Chem. 276: 17111-17116 [Abstract] [Full Text]