This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Telford, G.
Right arrow Articles by Pritchard, D. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Telford, G.
Right arrow Articles by Pritchard, D. I.

 Previous Article  |  Next Article 

Infect. Immun., Jan 1998, 36-42, Vol 66, No. 1
Copyright © 1998, American Society for Microbiology

The Pseudomonas aeruginosa quorum-sensing signal molecule N-(3- oxododecanoyl)-L-homoserine lactone has immunomodulatory activity

G Telford, D Wheeler, P Williams, PT Tomkins, P Appleby, H Sewell, GS Stewart, BW Bycroft and DI Pritchard
Department of Life Science, University of Nottingham, University Park, United Kingdom.

Diverse gram-negative bacterial cells communicate with each other by using diffusible N-acyl homoserine lactone (AHL) signal molecules to coordinate gene expression with cell population density. Accumulation of AHLs above a threshold concentration renders the population "quorate," and the appropriate target gene is activated. In pathogenic bacteria, such as Pseudomonas aeruginosa, AHL-mediated quorum sensing is involved in the regulation of multiple virulence determinants. We therefore sought to determine whether the immune system is capable of responding to these bacterial signal molecules. Consequently the immunomodulatory properties of the AHLs N-(3-oxododecanoyl)-L- homoserine lactone (OdDHL) and N-(3-oxohexanoyl)-L-homoserine lactone (OHHL) were evaluated in murine and human leukocyte immunoassays in vitro. OdDHL, but not OHHL, inhibited lymphocyte proliferation and tumor necrosis factor alpha production by lipopolysaccharide-stimulated macrophages. Furthermore, OdDHL simultaneously and potently down- regulated the production of IL-12, a Th-1-supportive cytokine. At high concentrations (>7 x 10(-5) M) OdDHL inhibited antibody production by keyhole limpet hemocyanin-stimulated spleen cells, but at lower concentrations (<7 x 10(-5) M), antibody production was stimulated, apparently by increasing the proportion of the immunoglobulin G1 (IgG1) isotype. OdDHL also promoted IgE production by interleukin-4-stimulated human peripheral blood mononuclear cells. These data indicate that OdDHL may influence the Th-1-Th-2 balance in the infected host and suggest that, in addition to regulating the expression of virulence determinants, OdDHL may contribute to the pathogenesis of P. aeruginosa infections by functioning as a virulence determinant per se.


This article has been cited by other articles:

  • Rennemeier, C., Frambach, T., Hennicke, F., Dietl, J., Staib, P. (2009). Microbial Quorum-Sensing Molecules Induce Acrosome Loss and Cell Death in Human Spermatozoa. Infect. Immun. 77: 4990-4997 [Abstract] [Full Text]  
  • Yang, L., Rybtke, M. T., Jakobsen, T. H., Hentzer, M., Bjarnsholt, T., Givskov, M., Tolker-Nielsen, T. (2009). Computer-Aided Identification of Recognized Drugs as Pseudomonas aeruginosa Quorum-Sensing Inhibitors. Antimicrob. Agents Chemother. 53: 2432-2443 [Abstract] [Full Text]  
  • Kimura, S., Tateda, K., Ishii, Y., Horikawa, M., Miyairi, S., Gotoh, N., Ishiguro, M., Yamaguchi, K. (2009). Pseudomonas aeruginosa Las quorum sensing autoinducer suppresses growth and biofilm production in Legionella species. Microbiology 155: 1934-1939 [Abstract] [Full Text]  
  • Skindersoe, M. E., Alhede, M., Phipps, R., Yang, L., Jensen, P. O., Rasmussen, T. B., Bjarnsholt, T., Tolker-Nielsen, T., Hoiby, N., Givskov, M. (2008). Effects of Antibiotics on Quorum Sensing in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 52: 3648-3663 [Abstract] [Full Text]  
  • Popat, R., Crusz, S. A., Diggle, S. P. (2008). The social behaviours of bacterial pathogens. Br Med Bull 87: 63-75 [Abstract] [Full Text]  
  • Jahoor, A., Patel, R., Bryan, A., Do, C., Krier, J., Watters, C., Wahli, W., Li, G., Williams, S. C., Rumbaugh, K. P. (2008). Peroxisome Proliferator-Activated Receptors Mediate Host Cell Proinflammatory Responses to Pseudomonas aeruginosa Autoinducer. J. Bacteriol. 190: 4408-4415 [Abstract] [Full Text]  
  • Murphy, B. S., Sundareshan, V., Cory, T. J., Hayes, D. Jr, Anstead, M. I., Feola, D. J. (2008). Azithromycin alters macrophage phenotype. J Antimicrob Chemother 61: 554-560 [Abstract] [Full Text]  
  • Epaulard, O., Derouazi, M., Margerit, C., Marlu, R., Filopon, D., Polack, B., Toussaint, B. (2008). Optimization of a Type III Secretion System-Based Pseudomonas aeruginosa Live Vector for Antigen Delivery. CVI 15: 308-313 [Abstract] [Full Text]  
  • Williams, P. (2007). Quorum sensing, communication and cross-kingdom signalling in the bacterial world. Microbiology 153: 3923-3938 [Abstract] [Full Text]  
  • Hoffmann, N., Lee, B., Hentzer, M., Rasmussen, T. B., Song, Z., Johansen, H. K., Givskov, M., Hoiby, N. (2007). Azithromycin Blocks Quorum Sensing and Alginate Polymer Formation and Increases the Sensitivity to Serum and Stationary-Growth-Phase Killing of Pseudomonas aeruginosa and Attenuates Chronic P. aeruginosa Lung Infection in Cftr / Mice. Antimicrob. Agents Chemother. 51: 3677-3687 [Abstract] [Full Text]  
  • Williams, P., Winzer, K., Chan, W. C, Camara, M. (2007). Look who's talking: communication and quorum sensing in the bacterial world. Phil Trans R Soc B 362: 1119-1134 [Abstract] [Full Text]  
  • Dong, Y.-H., Wang, L.-H., Zhang, L.-H. (2007). Quorum-quenching microbial infections: mechanisms and implications. Phil Trans R Soc B 362: 1201-1211 [Abstract] [Full Text]  
  • Manefield, M., Whiteley, A. S (2007). Acylated homoserine lactones in the environment: chameleons of bioactivity. Phil Trans R Soc B 362: 1235-1240 [Abstract] [Full Text]  
  • Diggle, S. P, Gardner, A., West, S. A, Griffin, A. S (2007). Evolutionary theory of bacterial quorum sensing: when is a signal not a signal?. Phil Trans R Soc B 362: 1241-1249 [Abstract] [Full Text]  
  • Christensen, L. D., Moser, C., Jensen, P. O., Rasmussen, T. B., Christophersen, L., Kjelleberg, S., Kumar, N., Hoiby, N., Givskov, M., Bjarnsholt, T. (2007). Impact of Pseudomonas aeruginosa quorum sensing on biofilm persistence in an in vivo intraperitoneal foreign-body infection model. Microbiology 153: 2312-2320 [Abstract] [Full Text]  
  • Zimmermann, S., Wagner, C., Muller, W., Brenner-Weiss, G., Hug, F., Prior, B., Obst, U., Hansch, G. M. (2006). Induction of neutrophil chemotaxis by the quorum-sensing molecule N-(3-oxododecanoyl)-L-homoserine lactone.. Infect. Immun. 74: 5687-5692 [Abstract] [Full Text]  
  • Miyairi, S., Tateda, K., Fuse, E. T., Ueda, C., Saito, H., Takabatake, T., Ishii, Y., Horikawa, M., Ishiguro, M., Standiford, T. J., Yamaguchi, K. (2006). Immunization with 3-oxododecanoyl-L-homoserine lactone-protein conjugate protects mice from lethal Pseudomonas aeruginosa lung infection.. J Med Microbiol 55: 1381-1387 [Abstract] [Full Text]  
  • Kravchenko, V. V., Kaufmann, G. F., Mathison, J. C., Scott, D. A., Katz, A. Z., Wood, M. R., Brogan, A. P., Lehmann, M., Mee, J. M., Iwata, K., Pan, Q., Fearns, C., Knaus, U. G., Meijler, M. M., Janda, K. D., Ulevitch, R. J. (2006). N-(3-Oxo-acyl)homoserine Lactones Signal Cell Activation through a Mechanism distinct from the Canonical Pathogen-associated Molecular Pattern Recognition Receptor Pathways. J. Biol. Chem. 281: 28822-28830 [Abstract] [Full Text]  
  • Atkinson, S., Chang, C.-Y., Sockett, R. E., Camara, M., Williams, P. (2006). Quorum Sensing in Yersinia enterocolitica Controls Swimming and Swarming Motility. J. Bacteriol. 188: 1451-1461 [Abstract] [Full Text]  
  • Qazi, S., Middleton, B., Muharram, S. H., Cockayne, A., Hill, P., O'Shea, P., Chhabra, S. R., Camara, M., Williams, P. (2006). N-Acylhomoserine Lactones Antagonize Virulence Gene Expression and Quorum Sensing in Staphylococcus aureus. Infect. Immun. 74: 910-919 [Abstract] [Full Text]  
  • Uroz, S., Chhabra, S. R., Camara, M., Williams, P., Oger, P., Dessaux, Y. (2005). N-Acylhomoserine lactone quorum-sensing molecules are modified and degraded by Rhodococcus erythropolis W2 by both amidolytic and novel oxidoreductase activities. Microbiology 151: 3313-3322 [Abstract] [Full Text]  
  • Visick, K. L., Fuqua, C. (2005). Decoding Microbial Chatter: Cell-Cell Communication in Bacteria. J. Bacteriol. 187: 5507-5519 [Full Text]  
  • Rasch, M., Andersen, J. B., Nielsen, K. F., Flodgaard, L. R., Christensen, H., Givskov, M., Gram, L. (2005). Involvement of Bacterial Quorum-Sensing Signals in Spoilage of Bean Sprouts. Appl. Environ. Microbiol. 71: 3321-3330 [Abstract] [Full Text]  
  • Kaper, J. B., Sperandio, V. (2005). Bacterial Cell-to-Cell Signaling in the Gastrointestinal Tract. Infect. Immun. 73: 3197-3209 [Full Text]  
  • Sadikot, R. T., Blackwell, T. S., Christman, J. W., Prince, A. S. (2005). Pathogen-Host Interactions in Pseudomonas aeruginosa Pneumonia. Am. J. Respir. Crit. Care Med. 171: 1209-1223 [Abstract] [Full Text]  
  • Ritchie, A. J., Jansson, A., Stallberg, J., Nilsson, P., Lysaght, P., Cooley, M. A. (2005). The Pseudomonas aeruginosa Quorum-Sensing Molecule N-3-(Oxododecanoyl)-L-Homoserine Lactone Inhibits T-Cell Differentiation and Cytokine Production by a Mechanism Involving an Early Step in T-Cell Activation. Infect. Immun. 73: 1648-1655 [Abstract] [Full Text]  
  • Rasmussen, T. B., Bjarnsholt, T., Skindersoe, M. E., Hentzer, M., Kristoffersen, P., Kote, M., Nielsen, J., Eberl, L., Givskov, M. (2005). Screening for Quorum-Sensing Inhibitors (QSI) by Use of a Novel Genetic System, the QSI Selector. J. Bacteriol. 187: 1799-1814 [Abstract] [Full Text]  
  • Bjarnsholt, T., Jensen, P. O., Burmolle, M., Hentzer, M., Haagensen, J. A. J., Hougen, H. P., Calum, H., Madsen, K. G., Moser, C., Molin, S., Hoiby, N., Givskov, M. (2005). Pseudomonas aeruginosa tolerance to tobramycin, hydrogen peroxide and polymorphonuclear leukocytes is quorum-sensing dependent. Microbiology 151: 373-383 [Abstract] [Full Text]  
  • Hooi, D. S. W., Bycroft, B. W., Chhabra, S. R., Williams, P., Pritchard, D. I. (2004). Differential Immune Modulatory Activity of Pseudomonas aeruginosa Quorum-Sensing Signal Molecules. Infect. Immun. 72: 6463-6470 [Abstract] [Full Text]  
  • Imamura, Y., Yanagihara, K., Mizuta, Y., Seki, M., Ohno, H., Higashiyama, Y., Miyazaki, Y., Tsukamoto, K., Hirakata, Y., Tomono, K., Kadota, J.-i., Kohno, S. (2004). Azithromycin Inhibits MUC5AC Production Induced by the Pseudomonas aeruginosa Autoinducer N-(3-Oxododecanoyl) Homoserine Lactone in NCI-H292 Cells. Antimicrob. Agents Chemother. 48: 3457-3461 [Abstract] [Full Text]  
  • Wu, H., Song, Z., Hentzer, M., Andersen, J. B., Molin, S., Givskov, M., Hoiby, N. (2004). Synthetic furanones inhibit quorum-sensing and enhance bacterial clearance in Pseudomonas aeruginosa lung infection in mice. J Antimicrob Chemother 53: 1054-1061 [Abstract] [Full Text]  
  • Williams, S. C., Patterson, E. K., Carty, N. L., Griswold, J. A., Hamood, A. N., Rumbaugh, K. P. (2004). Pseudomonas aeruginosa Autoinducer Enters and Functions in Mammalian Cells. J. Bacteriol. 186: 2281-2287 [Abstract] [Full Text]  
  • Teplitski, M., Chen, H., Rajamani, S., Gao, M., Merighi, M., Sayre, R. T., Robinson, J. B., Rolfe, B. G., Bauer, W. D. (2004). Chlamydomonas reinhardtii Secretes Compounds That Mimic Bacterial Signals and Interfere with Quorum Sensing Regulation in Bacteria. Plant Physiol. 134: 137-146 [Abstract] [Full Text]  
  • Sokol, P. A., Sajjan, U., Visser, M. B., Gingues, S., Forstner, J., Kooi, C. (2003). The CepIR quorum-sensing system contributes to the virulence of Burkholderia cenocepacia respiratory infections. Microbiology 149: 3649-3658 [Abstract] [Full Text]  
  • Jacobi, C. A., Bach, A., Eberl, L., Steidle, A., Heesemann, J. (2003). Detection of N-(3-Oxohexanoyl)-L-Homoserine Lactone in Mice Infected with Yersinia enterocolitica Serotype O8. Infect. Immun. 71: 6624-6626 [Abstract] [Full Text]  
  • Tateda, K., Ishii, Y., Horikawa, M., Matsumoto, T., Miyairi, S., Pechere, J. C., Standiford, T. J., Ishiguro, M., Yamaguchi, K. (2003). The Pseudomonas aeruginosa Autoinducer N-3-Oxododecanoyl Homoserine Lactone Accelerates Apoptosis in Macrophages and Neutrophils. Infect. Immun. 71: 5785-5793 [Abstract] [Full Text]  
  • Ritchie, A. J., Yam, A. O. W., Tanabe, K. M., Rice, S. A., Cooley, M. A. (2003). Modification of In Vivo and In Vitro T- and B-Cell-Mediated Immune Responses by the Pseudomonas aeruginosa Quorum-Sensing Molecule N-(3-Oxododecanoyl)-L-Homoserine Lactone. Infect. Immun. 71: 4421-4431 [Abstract] [Full Text]  
  • Sperandio, V., Torres, A. G., Jarvis, B., Nataro, J. P., Kaper, J. B. (2003). Bacteria-host communication: The language of hormones. Proc. Natl. Acad. Sci. USA 100: 8951-8956 [Abstract] [Full Text]  
  • Lesprit, P., Faurisson, F., Join-Lambert, O., Roudot-Thoraval, F., Foglino, M., Vissuzaine, C., Carbon, C. (2003). Role of the Quorum-sensing System in Experimental Pneumonia due to Pseudomonas aeruginosa in Rats. Am. J. Respir. Crit. Care Med. 167: 1478-1482 [Abstract] [Full Text]  
  • Ward, C, Camara, M, Forrest, I, Rutherford, R, Pritchard, G, Daykin, M, Hardman, A, de Soyza, A, Fisher, A J, Williams, P, Corris, P A (2003). Preliminary findings of quorum signal molecules in clinically stable lung allograft recipients. Thorax 58: 444-446 [Abstract] [Full Text]  
  • Mathesius, U., Mulders, S., Gao, M., Teplitski, M., Caetano-Anolles, G., Rolfe, B. G., Bauer, W. D. (2003). From the Cover: Extensive and specific responses of a eukaryote to bacterial quorum-sensing signals. Proc. Natl. Acad. Sci. USA 100: 1444-1449 [Abstract] [Full Text]  
  • Yates, E. A., Philipp, B., Buckley, C., Atkinson, S., Chhabra, S. R., Sockett, R. E., Goldner, M., Dessaux, Y., Camara, M., Smith, H., Williams, P. (2002). N-Acylhomoserine Lactones Undergo Lactonolysis in a pH-, Temperature-, and Acyl Chain Length-Dependent Manner during Growth of Yersinia pseudotuberculosis and Pseudomonas aeruginosa. Infect. Immun. 70: 5635-5646 [Abstract] [Full Text]  
  • Smith, R. S., Kelly, R., Iglewski, B. H., Phipps, R. P. (2002). The Pseudomonas Autoinducer N-(3-Oxododecanoyl) Homoserine Lactone Induces Cyclooxygenase-2 and Prostaglandin E2 Production in Human Lung Fibroblasts: Implications for Inflammation. J. Immunol. 169: 2636-2642 [Abstract] [Full Text]  
  • Taminiau, B., Daykin, M., Swift, S., Boschiroli, M.-L., Tibor, A., Lestrate, P., De Bolle, X., O'Callaghan, D., Williams, P., Letesson, J.-J. (2002). Identification of a Quorum-Sensing Signal Molecule in the Facultative Intracellular Pathogen Brucella melitensis. Infect. Immun. 70: 3004-3011 [Abstract] [Full Text]  
  • Laux, D. C., Corson, J. M., Givskov, M., Hentzer, M., Moller, A., Wosencroft, K. A., Olson, J. C., Krogfelt, K. A., Goldberg, J. B., Cohen, P. S. (2002). Lysophosphatidic acid inhibition of the accumulation of Pseudomonas aeruginosa PAO1 alginate, pyoverdin, elastase and LasA. Microbiology 148: 1709-1723 [Abstract] [Full Text]  
  • Schembri, M. A., Givskov, M., Klemm, P. (2002). An Attractive Surface: Gram-Negative Bacterial Biofilms. Sci Signal 2002: re6-re6 [Abstract] [Full Text]  
  • Erickson, D. L., Endersby, R., Kirkham, A., Stuber, K., Vollman, D. D., Rabin, H. R., Mitchell, I., Storey, D. G. (2002). Pseudomonas aeruginosa Quorum-Sensing Systems May Control Virulence Factor Expression in the Lungs of Patients with Cystic Fibrosis. Infect. Immun. 70: 1783-1790 [Abstract] [Full Text]  
  • Winzer, K., Hardie, K. R., Burgess, N., Doherty, N., Kirke, D., Holden, M. T. G., Linforth, R., Cornell, K. A., Taylor, A. J., Hill, P. J., Williams, P. (2002). LuxS: its role in central metabolism and the in vitro synthesis of 4-hydroxy-5-methyl-3(2H)-furanone. Microbiology 148: 909-922 [Abstract] [Full Text]  
  • Schneider, R., Lockatell, C. V., Johnson, D., Belas, R. (2002). Detection and mutation of a luxS-encoded autoinducer in Proteus mirabilis. Microbiology 148: 773-782 [Abstract] [Full Text]  
  • FRAY, R. G. (2002). Altering Plant-Microbe Interaction Through Artificially Manipulating Bacterial Quorum Sensing. ANN BOT (LOND) 89: 245-253 [Abstract] [Full Text]  
  • Smith, R. S., Harris, S. G., Phipps, R., Iglewski, B. (2002). The Pseudomonas aeruginosa Quorum-Sensing Molecule N-(3-Oxododecanoyl)Homoserine Lactone Contributes to Virulence and Induces Inflammation In Vivo. J. Bacteriol. 184: 1132-1139 [Abstract] [Full Text]  
  • Daniels, R., De Vos, D. E., Desair, J., Raedschelders, G., Luyten, E., Rosemeyer, V., Verreth, C., Schoeters, E., Vanderleyden, J., Michiels, J. (2002). The cin Quorum Sensing Locus of Rhizobium etli CNPAF512 Affects Growth and Symbiotic Nitrogen Fixation. J. Biol. Chem. 277: 462-468 [Abstract] [Full Text]  
  • Tateda, K., Comte, R., Pechere, J.-C., Köhler, T., Yamaguchi, K., Van Delden, C. (2001). Azithromycin Inhibits Quorum Sensing in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 45: 1930-1933 [Abstract] [Full Text]  
  • Wu, H., Song, Z., Givskov, M., Doring, G., Worlitzsch, D., Mathee, K., Rygaard, J., Høiby, N. (2001). Pseudomonas aeruginosa mutations in lasI and rhlI quorum sensing systems result in milder chronic lung infection. Microbiology 147: 1105-1113 [Abstract] [Full Text]  
  • Andersen, J. B., Heydorn, A., Hentzer, M., Eberl, L., Geisenberger, O., Christensen, B. B., Molin, S., Givskov, M. (2001). gfp-Based N-Acyl Homoserine-Lactone Sensor Systems for Detection of Bacterial Communication. Appl. Environ. Microbiol. 67: 575-585 [Abstract] [Full Text]  
  • Rasmussen, T. B., Manefield, M., Andersen, J. B., Eberl, L., Anthoni, U., Christophersen, C., Steinberg, P., Kjelleberg, S., Givskov, M. (2000). How Delisea pulchra furanones affect quorum sensing and swarming motility in Serratia liquefaciens MG1. Microbiology 146: 3237-3244 [Abstract] [Full Text]  
  • Wu, H., Song, Z., Hentzer, M., Andersen, J. B., Heydorn, A., Mathee, K., Moser, C., Eberl, L., Molin, S., Høiby, N., Givskov, M. (2000). Detection of N-acylhomoserine lactones in lung tissues of mice infected with Pseudomonas aeruginosa. Microbiology 146: 2481-2493 [Abstract] [Full Text]  
  • de Kievit, T. R., Iglewski, B. H. (2000). Bacterial Quorum Sensing in Pathogenic Relationships. Infect. Immun. 68: 4839-4849 [Full Text]  
  • Forsyth, M. H., Cover, T. L. (2000). Intercellular Communication in Helicobacter pylori: luxS Is Essential for the Production of an Extracellular Signaling Molecule. Infect. Immun. 68: 3193-3199 [Abstract] [Full Text]  
  • Saleh, A., Figarella, C., Kammouni, W., Marchand-Pinatel, S., Lazdunski, A., Tubul, A., Brun, P., Merten, M. D. (1999). Pseudomonas aeruginosa Quorum-Sensing Signal Molecule N-(3-Oxododecanoyl)-L-Homoserine Lactone Inhibits Expression of P2Y Receptors in Cystic Fibrosis Tracheal Gland Cells. Infect. Immun. 67: 5076-5082 [Abstract] [Full Text]  
  • Wilson, M., Seymour, R., Henderson, B. (1998). Bacterial Perturbation of Cytokine Networks. Infect. Immun. 66: 2401-2409 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»