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Infection and Immunity, October 1998, p. 4624-4632, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Invasion by Salmonella typhimurium Induces Increased Expression of the LMP, MECL, and PA28 Proteasome Genes and Changes in the Peptide Repertoire of HLA-B27

Walter P. Maksymowych,1,* Takashi Ikawa,2 Akihiro Yamaguchi,2 Makoto Ikeda,2 Darrin McDonald,1 Leila Laouar,1 Riitta Lahesmaa,3 Naoto Tamura,2 Arranny Khuong,1 David T. Y. Yu,2 and Kevin P. Kane1

Departments of Medicine and Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada,1 and Department of Medicine, University of California, Los Angeles,2 and Roche Bioscience, Palo Alto,3 California

Received 10 November 1997/Returned for modification 26 January 1998/Accepted 23 June 1998

We have analyzed proteasomal adaptation and associated changes in the B27-bound peptide repertoire in response to cellular invasion with Salmonella. The peptide repertoire of HLA-B27 complexes was analyzed by two different methods: (i) high-pressure liquid chromatography (HPLC) profiles of newly synthesized peptides eluted from B27 following metabolic labeling with arginine and (ii) reactivities with two B27 monoclonal antibodies, Ye-2 and B27.M2, sensitive to peptide-induced conformational changes. LMP, MECL, and PA28 expression was analyzed by reverse transcription-PCR (RT-PCR) of mRNA and by Western blot analysis for LMP2. Invasion of HLA-B27-transfected HeLa cells by Salmonella typhimurium induced significant changes in the reactivities of HLA-B27 with these two antibodies, which was accompanied by significant quantitative and qualitative changes in the HPLC profile of peptides eluted from HLA-B27. We also observed increases in the RT-PCR values for the LMP2, LMP7, and MECL proteasome subunit genes, as well as the proteasomal activator PA28alpha and -beta genes, and increased expression of the LMP2 protein by Western blotting. Upregulation of LMP2, but not LMP7, gene expression showed a close correlation with the changes in antibody reactivities observed upon bacterial invasion. We observed similar changes in reactivity with the Ye-2 or the B27.M2 antibody of lymphoblastoid cells upon gamma interferon treatment, which significantly correlated with the increased RT-PCR values for the LMP2 gene. This was accompanied by consistent HPLC profile changes for eluted peptides. Thus, Salmonella invasion leads to serologically recognizable changes in the B27-bound peptide repertoire, which may include peptides of host origin potentially through modulation of proteasome LMP2 subunit expression and, as a consequence, proteasomal activities.

* Corresponding author. Mailing address: 562 Heritage Medical Research Center, University of Alberta, Edmonton, Alberta, Canada T6G 2S2. Phone: (403) 492-1964. Fax: (403) 492-6055. E-mail: maksymow{at}gpu.srv.ualberta.ca.

Infection and Immunity, October 1998, p. 4624-4632, Vol. 66, No. 10
0019-9567/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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